RchiOBHm_Chr5g0065511

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000624
Physical Location & Seq
Forward (+)
71460355 .. 71462131
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34137

Sequence Viewer

Length: 1647 bp
ATGGAATCAAAACCCTGTATGTTCCATGACCCTTTGGCTCTTCAAGCCACTGTGGAGTCTCTTCATGAGGTTATTGGAGGAGGGAAGTACCCAACTTCCCAAGACCTAAACCCTCTTTGTGTTGATCAAGATGTGTATGGAAATGGAAATGGGATGGCGGGTTCTTATAGGAGTGAGCTAATGTTTCTTCCTAGAGGTCAGTTGATTAGTGCAGAGGGCAACAAGCCGTATGCTGTGGCGGATAGAAAGGCTGGAGATGTGGTAGTCCCGACCCAGAAGAATGGGAGTGAGCATTTTCGTGTTGTTTCTAGAGGAAAGAAAAATCATCAAAGGGAAGATATAGATTTGGAGGATGGGAGGAGTAACAAGCAATCGACGGTTTATATGGATGGTGAGGAGGGTGAGCTGTGTGATATTTTTGACAAGGTGTTGCTCTCCAAGCCTATCAAGAGTAAAGACGATCAAGTCAGTCAGAATGGAGCAAACAATAGCCTGCAGCAGGATGGGCTATCTGTTGGAATTGGTAATGGGAAGGTTTGCGGCAAGAATCAAGGCAACAAGAAGGAAGTGATAGATTTGAGGGCTCTTCTGATTTCATGTGCAGTGGCTGTATCTGTTGATGACCGCAAGACTGCTAGTGAGCTACTAAAGCAGATTATGCAGCACTCTTCCCCATTTGGTGATTGTACTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTGAAGCGCGGTTGGCTGGCACAGGAACTCAAATATATACTGCTTTAACTACTAAACGAATGTCAGCTGCTGATTCGCTGAAGTTTTACCAAGCTTATATTGCAGTCTGCCCGTTCATGAAGTTGTTGATGATCTTTGCAAACGATATGATTTTAAAAGCTGCTGAGAAAGCAGAAACACTACATATAATAGATTTTGGTATCCGTTATGGTTTTCAATGGCCAGCTCTCATTCATTGCCTCTCAAGAAGACCTGGTGGACCTCCGAAATTACGCATTACAGGGTTAGAGTTTCCCTCAAGTGGTTTTCGACCAGAAGAAGGAGTCCAAGAGACAGGGCATCGCTTGGCAAAGTATTGTAGGGGATTTAATGTTCATTTTGAGTACCATGGCATAGCCAAAAAATGGGAAACTCTCCAAGTTGATGACCTTAAAGTTCAAAGAAATGAGGTGCTTGCTGTTAATTGTCTCAACCGATTCAAGTACCTCCTTGATGAGACTGTTGTGGTAAACAGTCCAAGGGATACTGTTCTAAATTTAATTAGGAATTTGAATCCTGATGTCTTTGTCCAAAGTGTTGTTAATGGATCCCATCATGCACCCTTCTTTGTTCCACGCTTCCGAGAGGCACTATTCCACTTCTCTGCATTGTTTGATATGTTGGATACCAATTTGCCTCGTGAAGATCAGATGAGATTGGGGTTTGAGAAAGAGCCAGAAACATACAAGCAGTGGCAGATTCGAAACATGAGGGCTGGGTTCAGACAGCTGCCATTGGACCGTGAAGTCATGAACAAAATAAAGGATAAGGTAAAAATGGGGTACCACCCTTCTTTTGTGGTTGATGAAGATGGCCGCTGGCTGCTGCATGAATGGAAGGGCAGAATTATGTATGCTTCTGCCTGTTGGGCACCATCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

548

Amino Acids

61.77

Weight (kDa)

8.32

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 193 - 543 1.9e-100 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 464, 1505
Acc65I GGTACC 1 cut(s) 1542
AccB1I GGYRCC 2 cut(s) 1542, 1630
AccB7I CCANNNNNTGG 1 cut(s) 1125
AccII CGCG 1 cut(s) 730
AciI CCGC 6 cut(s) 158, 239, 540, 625, 730, 1576
AclWI GGATC 2 cut(s) 1302, 1315
AcoI YGGCCR 2 cut(s) 941, 1573
AcsI RAATTY 2 cut(s) 1255, 1267
AcuI CTGAAG 1 cut(s) 821
AfaI GTAC 5 cut(s) 89, 688, 1106, 1205, 1544
AfiI CCNNNNNNNGG 4 cut(s) 499, 1022, 1040, 1125
AgsI TTSAA 6 cut(s) 44, 725, 938, 1160, 1201, 1273
AjnI CCWGG 1 cut(s) 973
AloI GAACNNNNNNTCC 4 cut(s) 145, 177, 1077, 1109
AluBI AGCT 8 cut(s) 178, 406, 643, 788, 815, 881, 947, 1489
AluI AGCT 8 cut(s) 178, 406, 643, 788, 815, 881, 947, 1489
Alw26I GTCTC 4 cut(s) 63, 1046, 1193, 1211
AlwI GGATC 2 cut(s) 1302, 1315
AlwNI CAGNNNCTG 2 cut(s) 608, 791
AoxI GGCC 2 cut(s) 941, 1573
ApeKI GCWGC 7 cut(s) 496, 661, 788, 881, 1489, 1582, 1585
ApoI RAATTY 2 cut(s) 1255, 1267
Asp718I GGTACC 1 cut(s) 1542
AspLEI GCGC 1 cut(s) 730
AspS9I GGNCC 2 cut(s) 980, 1498
AsuHPI GGTGA 3 cut(s) 404, 413, 692
AsuII TTCGAA 1 cut(s) 1462
AvaII GGWCC 2 cut(s) 980, 1498
BaeGI GKGCMC 1 cut(s) 1633
BalI TGGCCA 1 cut(s) 943
BamHI GGATCC 1 cut(s) 1307
BanI GGYRCC 2 cut(s) 1542, 1630
BanII GRGCYC 1 cut(s) 586
BauI CACGAG 1 cut(s) 1398
BbsI GAAGAC 1 cut(s) 976
BbvI GCAGC 7 cut(s) 508, 673, 775, 868, 1476, 1569, 1572
BccI CCATC 7 cut(s) 148, 347, 383, 497, 1320, 1565, 1642
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 1 cut(s) 975
BciVI GTATCC 3 cut(s) 932, 1237, 1378
BclI TGATCA 1 cut(s) 124
BcoDI GTCTC 4 cut(s) 63, 1046, 1193, 1211
BfaI CTAG 4 cut(s) 192, 309, 636, 1638
BfmI CTRYAG 1 cut(s) 494
BfuI GTATCC 3 cut(s) 932, 1237, 1378
BglI GCCNNNNNGGC 1 cut(s) 1628
BisI GCNGC 9 cut(s) 497, 541, 662, 789, 882, 1490, 1576, 1583, 1586
BlsI GCNGC 9 cut(s) 498, 542, 663, 790, 883, 1491, 1577, 1584, 1587
Bme1390I CCNGG 1 cut(s) 975
Bme18I GGWCC 2 cut(s) 980, 1498
BmgT120I GGNCC 2 cut(s) 980, 1498
BmiI GGNNCC 3 cut(s) 1309, 1544, 1632
BmrFI CCNGG 1 cut(s) 975
BmsI GCATC 1 cut(s) 1069
BpiI GAAGAC 1 cut(s) 976
BplI GAGNNNNNCTC 4 cut(s) 685, 717, 1001, 1033
BpmI CTGGAG 1 cut(s) 273
Bpu14I TTCGAA 1 cut(s) 1462
BpuEI CTTGAG 2 cut(s) 949, 1003
BsaJI CCNNGG 2 cut(s) 1108, 1238
BsaXI ACNNNNNCTCC 2 cut(s) 246, 276
Bsc4I CCNNNNNNNGG 4 cut(s) 499, 1022, 1040, 1125
Bse3DI GCAATG 2 cut(s) 703, 955
BseBI CCWGG 1 cut(s) 975
BseDI CCNNGG 2 cut(s) 1108, 1238
BseGI GGATG 4 cut(s) 159, 358, 394, 508
BseLI CCNNNNNNNGG 4 cut(s) 499, 1022, 1040, 1125
BseMI GCAATG 2 cut(s) 703, 955
BseMII CTCAG 2 cut(s) 704, 876
BseRI GAGGAG 3 cut(s) 93, 373, 410
BseSI GKGCMC 1 cut(s) 1633
BseXI GCAGC 7 cut(s) 508, 673, 775, 868, 1476, 1569, 1572
BseYI CCCAGC 1 cut(s) 1475
BsgI GTGCAG 2 cut(s) 231, 621
Bsh1236I CGCG 1 cut(s) 730
BshFI GGCC 2 cut(s) 943, 1575
BshNI GGYRCC 2 cut(s) 1542, 1630
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 4 cut(s) 499, 1022, 1040, 1125
BsmAI GTCTC 4 cut(s) 63, 1046, 1193, 1211
BsmFI GGGAC 1 cut(s) 251
BsnI GGCC 2 cut(s) 943, 1575
Bsp119I TTCGAA 1 cut(s) 1462
Bsp1286I GDGCHC 2 cut(s) 586, 1633
Bsp143I GATC 5 cut(s) 124, 460, 852, 1307, 1405
Bsp19I CCATGG 1 cut(s) 1108
BspACI CCGC 6 cut(s) 158, 239, 540, 625, 730, 1576
BspANI GGCC 2 cut(s) 943, 1575
BspCNI CTCAG 2 cut(s) 703, 877
BspFNI CGCG 1 cut(s) 730
BspHI TCATGA 3 cut(s) 64, 837, 1509
BspLI GGNNCC 3 cut(s) 1309, 1544, 1632
BspMAI CTGCAG 1 cut(s) 498
BspPI GGATC 2 cut(s) 1302, 1315
BspQI GCTCTTC 2 cut(s) 45, 591
BspT104I TTCGAA 1 cut(s) 1462
BspT107I GGYRCC 2 cut(s) 1542, 1630
BsrDI GCAATG 2 cut(s) 703, 955
BssECI CCNNGG 2 cut(s) 1108, 1238
BssMI GATC 5 cut(s) 124, 460, 852, 1307, 1405
BssSI CACGAG 1 cut(s) 1398
BssT1I CCWWGG 2 cut(s) 1108, 1238
Bst2BI CACGAG 1 cut(s) 1398
Bst2UI CCWGG 1 cut(s) 975
Bst4CI ACNGT 6 cut(s) 52, 379, 1222, 1235, 1249, 1502
Bst6I CTCTTC 4 cut(s) 45, 66, 591, 673
BstAPI GCANNNNNTGC 1 cut(s) 658
BstBI TTCGAA 1 cut(s) 1462
BstC8I GCNNGC 5 cut(s) 494, 739, 945, 1176, 1580
BstDEI CTNAG 2 cut(s) 690, 885
BstDSI CCRYGG 1 cut(s) 1108
BstENI CCTNNNNNAGG 1 cut(s) 497
BstF5I GGATG 4 cut(s) 159, 358, 394, 508
BstFNI CGCG 1 cut(s) 730
BstHHI GCGC 1 cut(s) 730
BstKTI GATC 5 cut(s) 127, 463, 855, 1310, 1408
BstMAI GTCTC 4 cut(s) 63, 1046, 1193, 1211
BstMBI GATC 5 cut(s) 124, 460, 852, 1307, 1405
BstNI CCWGG 1 cut(s) 975
BstSCI CCNGG 1 cut(s) 973
BstSFI CTRYAG 1 cut(s) 494
BstSLI GKGCMC 1 cut(s) 1633
BstUI CGCG 1 cut(s) 730
BstV1I GCAGC 7 cut(s) 508, 673, 775, 868, 1476, 1569, 1572
BstV2I GAAGAC 1 cut(s) 976
BstX2I RGATCY 1 cut(s) 1307
BstXI CCANNNNNNTGG 1 cut(s) 281
BstYI RGATCY 1 cut(s) 1307
BsuI GTATCC 3 cut(s) 932, 1237, 1378
BsuRI GGCC 2 cut(s) 943, 1575
BtgI CCRYGG 1 cut(s) 1108
BtgZI GCGATG 1 cut(s) 1046
BtsCI GGATG 4 cut(s) 159, 358, 394, 508
BtsI GCAGTG 2 cut(s) 609, 1457
BtsIMutI CAGTG 3 cut(s) 48, 609, 1457
Cac8I GCNNGC 5 cut(s) 494, 739, 945, 1176, 1580
CaiI CAGNNNCTG 2 cut(s) 608, 791
CciI TCATGA 3 cut(s) 64, 837, 1509
CfoI GCGC 1 cut(s) 730
Cfr13I GGNCC 2 cut(s) 980, 1498
CsiI ACCWGGT 1 cut(s) 973
Csp6I GTAC 5 cut(s) 88, 687, 1105, 1204, 1543
CviAII CATG 9 cut(s) 26, 65, 597, 838, 1109, 1316, 1468, 1510, 1589
CviQI GTAC 5 cut(s) 88, 687, 1105, 1204, 1543
DdeI CTNAG 2 cut(s) 690, 885
DpnI GATC 5 cut(s) 126, 462, 854, 1309, 1407
DpnII GATC 5 cut(s) 124, 460, 852, 1307, 1405
DraI TTTAAA 1 cut(s) 876
DrdI GACNNNNNNGTC 2 cut(s) 464, 1505
DseDI GACNNNNNNGTC 2 cut(s) 464, 1505
EaeI YGGCCR 2 cut(s) 941, 1573
Eam1104I CTCTTC 4 cut(s) 45, 66, 591, 673
EarI CTCTTC 4 cut(s) 45, 66, 591, 673
EciI GGCGGA 1 cut(s) 254
Eco130I CCWWGG 2 cut(s) 1108, 1238
Eco24I GRGCYC 1 cut(s) 586
Eco47I GGWCC 2 cut(s) 980, 1498
Eco57I CTGAAG 1 cut(s) 821
EcoNI CCTNNNNNAGG 1 cut(s) 497
EcoRII CCWGG 1 cut(s) 973
EcoT14I CCWWGG 2 cut(s) 1108, 1238
EcoT38I GRGCYC 1 cut(s) 586
ErhI CCWWGG 2 cut(s) 1108, 1238
FaeI CATG 9 cut(s) 29, 68, 600, 841, 1112, 1319, 1471, 1513, 1592
FaqI GGGAC 1 cut(s) 251
FatI CATG 9 cut(s) 25, 64, 596, 837, 1108, 1315, 1467, 1509, 1588
FauI CCCGC 1 cut(s) 151
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 9 cut(s) 497, 541, 662, 789, 882, 1490, 1576, 1583, 1586
FokI GGATG 4 cut(s) 166, 365, 401, 515
FriOI GRGCYC 1 cut(s) 586
Fsp4HI GCNGC 9 cut(s) 497, 541, 662, 789, 882, 1490, 1576, 1583, 1586
FspBI CTAG 4 cut(s) 192, 309, 636, 1638
GlaI GCGC 1 cut(s) 729
GluI GCNGC 9 cut(s) 497, 541, 662, 789, 882, 1490, 1576, 1583, 1586
GsaI CCCAGC 1 cut(s) 1479
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 2 cut(s) 943, 1575
HhaI GCGC 1 cut(s) 730
Hin1II CATG 9 cut(s) 29, 68, 600, 841, 1112, 1319, 1471, 1513, 1592
Hin6I GCGC 1 cut(s) 728
HinP1I GCGC 1 cut(s) 728
HindIII AAGCTT 1 cut(s) 813
HinfI GANTC 8 cut(s) 5, 56, 547, 794, 1044, 1197, 1273, 1459
HphI GGTGA 3 cut(s) 404, 413, 692
Hpy166II GTNNAC 2 cut(s) 980, 1231
Hpy188I TCNGA 7 cut(s) 474, 591, 693, 987, 1343, 1410, 1484
Hpy8I GTNNAC 2 cut(s) 980, 1231
Hpy99I CGWCG 1 cut(s) 379
HpyAV CCTTC 6 cut(s) 526, 556, 1034, 1333, 1560, 1591
HpyCH4III ACNGT 6 cut(s) 52, 379, 1222, 1235, 1249, 1502
HpyF3I CTNAG 2 cut(s) 690, 885
Hsp92II CATG 9 cut(s) 29, 68, 600, 841, 1112, 1319, 1471, 1513, 1592
HspAI GCGC 1 cut(s) 728
KpnI GGTACC 1 cut(s) 1546
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 5 cut(s) 124, 460, 852, 1307, 1405
LguI GCTCTTC 2 cut(s) 45, 591
LmnI GCTCC 1 cut(s) 479
Lsp1109I GCAGC 7 cut(s) 508, 673, 775, 868, 1476, 1569, 1572
LweI GCATC 1 cut(s) 1069
MabI ACCWGGT 1 cut(s) 973
MaeI CTAG 4 cut(s) 192, 309, 636, 1638
MaeIII GTNAC 1 cut(s) 362
MalI GATC 5 cut(s) 126, 462, 854, 1309, 1407
MboI GATC 5 cut(s) 124, 460, 852, 1307, 1405
MflI RGATCY 1 cut(s) 1307
MhlI GDGCHC 2 cut(s) 586, 1633
MlsI TGGCCA 1 cut(s) 943
MluCI AATT 8 cut(s) 519, 989, 1183, 1255, 1260, 1267, 1390, 1605
MluNI TGGCCA 1 cut(s) 943
MlyI GAGTC 2 cut(s) 65, 1053
MmeI TCCRAC 2 cut(s) 496, 1362
Mox20I TGGCCA 1 cut(s) 943
MscI TGGCCA 1 cut(s) 943
MseI TTAA 7 cut(s) 767, 875, 1089, 1152, 1182, 1259, 1302
MslI CAYNNNNRTG 1 cut(s) 297
Msp20I TGGCCA 1 cut(s) 943
MspA1I CMGCKG 3 cut(s) 788, 1489, 1578
MspR9I CCNGG 1 cut(s) 975
MvaI CCWGG 1 cut(s) 975
MvnI CGCG 1 cut(s) 730
NcoI CCATGG 1 cut(s) 1108
NdeII GATC 5 cut(s) 124, 460, 852, 1307, 1405
NlaIII CATG 9 cut(s) 29, 68, 600, 841, 1112, 1319, 1471, 1513, 1592
NlaIV GGNNCC 3 cut(s) 1309, 1544, 1632
NspV TTCGAA 1 cut(s) 1462
PagI TCATGA 3 cut(s) 64, 837, 1509
PciSI GCTCTTC 2 cut(s) 45, 591
PfeI GAWTC 6 cut(s) 5, 547, 794, 1197, 1273, 1459
PflMI CCANNNNNTGG 1 cut(s) 1125
PkrI GCNGC 9 cut(s) 498, 542, 663, 790, 883, 1491, 1577, 1584, 1587
PleI GAGTC 2 cut(s) 64, 1052
PpsI GAGTC 2 cut(s) 64, 1052
Psp6I CCWGG 1 cut(s) 973
PspFI CCCAGC 1 cut(s) 1475
PspGI CCWGG 1 cut(s) 973
PspN4I GGNNCC 3 cut(s) 1309, 1544, 1632
PspPI GGNCC 2 cut(s) 980, 1498
PstI CTGCAG 1 cut(s) 498
PstNI CAGNNNCTG 2 cut(s) 608, 791
PsuI RGATCY 1 cut(s) 1307
PvuII CAGCTG 2 cut(s) 788, 1489
RsaI GTAC 5 cut(s) 89, 688, 1106, 1205, 1544
RsaNI GTAC 5 cut(s) 88, 687, 1105, 1204, 1543
RseI CAYNNNNRTG 1 cut(s) 297
SapI GCTCTTC 2 cut(s) 45, 591
SaqAI TTAA 7 cut(s) 767, 875, 1089, 1152, 1182, 1259, 1302
SatI GCNGC 9 cut(s) 497, 541, 662, 789, 882, 1490, 1576, 1583, 1586
Sau3AI GATC 5 cut(s) 124, 460, 852, 1307, 1405
Sau96I GGNCC 2 cut(s) 980, 1498
SchI GAGTC 2 cut(s) 65, 1053
ScrFI CCNGG 1 cut(s) 975
SduI GDGCHC 2 cut(s) 586, 1633
SexAI ACCWGGT 1 cut(s) 973
SfaNI GCATC 1 cut(s) 1069
SfcI CTRYAG 1 cut(s) 494
SfuI TTCGAA 1 cut(s) 1462
SinI GGWCC 2 cut(s) 980, 1498
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 2 cut(s) 964, 1018
SmoI CTYRAG 2 cut(s) 964, 1018
Sse9I AATT 8 cut(s) 519, 989, 1183, 1255, 1260, 1267, 1390, 1605
SsiI CCGC 6 cut(s) 158, 239, 540, 625, 730, 1576
SspMI CTAG 4 cut(s) 192, 309, 636, 1638
StyD4I CCNGG 1 cut(s) 973
StyI CCWWGG 2 cut(s) 1108, 1238
TaaI ACNGT 6 cut(s) 52, 379, 1222, 1235, 1249, 1502
TaqI TCGA 3 cut(s) 374, 1030, 1462
TasI AATT 8 cut(s) 519, 989, 1183, 1255, 1260, 1267, 1390, 1605
TatI WGTACW 1 cut(s) 686
TauI GCSGC 2 cut(s) 543, 1578
TfiI GAWTC 6 cut(s) 5, 547, 794, 1197, 1273, 1459
Tru1I TTAA 7 cut(s) 767, 875, 1089, 1152, 1182, 1259, 1302
Tru9I TTAA 7 cut(s) 767, 875, 1089, 1152, 1182, 1259, 1302
TscAI CASTG 3 cut(s) 55, 609, 1457
TseI GCWGC 7 cut(s) 496, 661, 788, 881, 1489, 1582, 1585
TspDTI ATGAA 9 cut(s) 53, 585, 826, 854, 944, 1085, 1526, 1581, 1605
TspGWI ACGGA 1 cut(s) 914
TspRI CASTG 3 cut(s) 55, 609, 1457
Van91I CCANNNNNTGG 1 cut(s) 1125
VpaK11BI GGWCC 2 cut(s) 980, 1498
XagI CCTNNNNNAGG 1 cut(s) 497
XapI RAATTY 2 cut(s) 1255, 1267
XbaI TCTAGA 1 cut(s) 308
XspI CTAG 4 cut(s) 192, 309, 636, 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.