FvH4_5g17360

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000624
Physical Location & Seq
Forward (+)
9925844 .. 9927697
1854 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17360.t1

Sequence Viewer

Length: 1854 bp
ATGGATTCATCTTTCTCTGAATATCCCAATTTCGAAGATCAAACCCTTCTACTCGATATGTTGAACGAGTACCCAAGTACTGACTCTTTTGATGATCTTAGCTTTTTGGACCCAAACCCCATGAACTCTGCCCTGTCTTCGAGCTTTAGCCCTGTAGCAGATGATGGTGAGTTTTCTGATAGTGTGATCAAGCACATAAACAAGGTGCTTATGGAGGAGGACATGGAGCCAAAGCCGTGCATGTTCCATGACCCTTTGGTTCTTCAAGCCACTGAGGAGTCTCTTTATGAGGTTATTGAAGGAGGGAAGTGCTCAACTTCCCAAGACCAAAACCCTCATTGGGTTGATCAAAATGAGTATGGAAATGGAAATGGGATGGTGGGTTCTTATAGGAGTGAGCTTATGGTTCCTGATGCGGTTAGTAAGAACGAATTGTTAGTGCAATTTAACCGAGGGTTAGAGGATGGTTGTAAGTTTCTTCCTAGAGGTCAGTTGATTACTGCAGAGGGCAACAAGACATATGATGTGGCGAATAGTAAGGCAGGAAATGTGGTCGTTACGACCCAGAAGAATGACAGTGAGCATGTTCATGTTGGTTCAAGAGGAAAGAAGAGTCATCAGAGGGAGGAGATAGATTTGGAGGATGGGAGGATTAACAAGCAATCGGCGGTTTATATGGATGATGTGGAGGGTGAGCTGTCTGATAGTTTCGGGAAGGCGTTGCTCAGCGGTAAGGCTGTCGAGAGTAAAGAGGATCAAGTCTGTCAGAATGGAGCTAACAATGGCCTGCAGCAGGATGTGCTAGTCAGTCAGAAGGCTTGTGGCATGAAACGAGGCAACAAGAAGGAAGTGATAGATTTGAGGCGTCTTTTGATTTCATGTGCAGAAGCTGTTGCTGTTGATGATCGAGGGACTGCTAGTGAGCTACTGAAGCAAATTAGGCAGCACTCTTCCCCATTTGGTGATTATACTCAGAGGTTGGCTCATTGTTTTGCAAATGCTCTTGAAGCACGGTTGGCTGGCACTGGAAGTGAACTATATACTACTTTGGCTACTAAACGAGCGTCAGCTGCAGATATCTCAAAGTTTTACCGAGCTTATATTGCAGTCTGCCCGTTCATGAAGATGTTGATGATTTTTGCAAACCATATGATTATAAAAGCCGCTGAGAAAGCAGAAACACTTCATATATTAGATTTTGGTATCCGCCAAGGTTTTCAATGGCCTGCTCTCATTCATTGCCTCTCAAGAAGACCTGGTGGACCTCCTAAATTACGCATTACAGGAGTAGAGCTTCCCTTAAGTGGTTGTCGAGCAGAAGAAACAGTCCAAGAGACAGGGCATCGATTGGCAAAGTATTGTAATCGATTTAGTGTTCCTTTTAAGTATTATGCCATATCCAGAAAATGGGAAACTCTCCAACCTGATGACCTTAAAGTTAAAAGAAATGAGGTGCTTGCTGTTAATTGTCTCTGCCGATTTAAGTACCTCCTTGATGAGACAGTTATGGTAAACAGTCCAAGGGATACTGTTCTAAATTTGATTAGGAAGTTGAATCCTGATATTTTTGTCCATAGTATTGTTAATGGATCCCATGATGCCCCCTACTTTGTTCCACGCTTCCGAGAGGCACTATTCCACTTCTCTGCATTGTTTGATGTGTTGGATACTACTTTTCCTCTCGAAGATCAGATGAGATTGGGGTTTGAGAAAGAGTTTATTGGACGTGAAGTTCTGAATATAGTTGCTTGTGAGGGCACTAAAAGAATTGCGAGGCCCGAAACATATAAGCAGTGGCAGATTCGAAACAGGAGGCTGGTTTCAGACAGCTGCCATTGGACTATGAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

618

Amino Acids

69.56

Weight (kDa)

5.75

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 287 - 602 4.2e-102 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1157
AccB7I CCANNNNNTGG 1 cut(s) 1407
AciI CCGC 5 cut(s) 416, 668, 729, 1164, 1207
AclWI GGATC 3 cut(s) 762, 1584, 1597
AcsI RAATTY 1 cut(s) 1537
AcuI CTGAAG 1 cut(s) 950
AcyI GRCGYC 1 cut(s) 865
AfaI GTAC 3 cut(s) 71, 79, 1487
AfiI CCNNNNNNNGG 4 cut(s) 340, 793, 1304, 1407
AflII CTTAAG 1 cut(s) 1300
AgsI TTSAA 7 cut(s) 64, 266, 299, 600, 1007, 1220, 1555
AjiI CACGTC 1 cut(s) 1727
AjnI CCWGG 1 cut(s) 1255
AloI GAACNNNNNNTCC 4 cut(s) 367, 399, 1716, 1748
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 4 cut(s) 285, 1328, 1475, 1493
AlwI GGATC 3 cut(s) 762, 1584, 1597
AlwNI CAGNNNCTG 1 cut(s) 890
AoxI GGCC 3 cut(s) 784, 1223, 1775
ApeKI GCWGC 4 cut(s) 790, 943, 1070, 1830
ApoI RAATTY 1 cut(s) 1537
Asp700I GAANNNNTTC 1 cut(s) 1182
AspS9I GGNCC 3 cut(s) 109, 1262, 1776
AsuHPI GGTGA 3 cut(s) 179, 704, 974
AsuII TTCGAA 2 cut(s) 33, 1804
AvaII GGWCC 2 cut(s) 109, 1262
BaeGI GKGCMC 1 cut(s) 1760
BamHI GGATCC 1 cut(s) 1589
BbsI GAAGAC 2 cut(s) 129, 1258
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 4 cut(s) 802, 955, 1057, 1817
BccI CCATC 4 cut(s) 158, 370, 458, 638
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 1 cut(s) 1257
BciVI GTATCC 3 cut(s) 1214, 1519, 1660
BclI TGATCA 2 cut(s) 186, 346
BcoDI GTCTC 4 cut(s) 285, 1328, 1475, 1493
BfaI CTAG 3 cut(s) 483, 803, 918
BfmI CTRYAG 4 cut(s) 153, 501, 788, 1071
BfrI CTTAAG 1 cut(s) 1300
BfuI GTATCC 3 cut(s) 1214, 1519, 1660
BisI GCNGC 5 cut(s) 791, 944, 1071, 1164, 1831
BlpI GCTNAGC 1 cut(s) 725
BlsI GCNGC 5 cut(s) 792, 945, 1072, 1165, 1832
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 1257
Bme18I GGWCC 2 cut(s) 109, 1262
BmgBI CACGTC 1 cut(s) 1727
BmgT120I GGNCC 3 cut(s) 109, 1262, 1776
BmiI GGNNCC 4 cut(s) 111, 228, 408, 1591
BmrFI CCNGG 1 cut(s) 1257
BmsI GCATC 3 cut(s) 403, 1351, 1588
BpiI GAAGAC 2 cut(s) 129, 1258
BplI GAGNNNNNCTC 2 cut(s) 967, 999
Bpu1102I GCTNAGC 1 cut(s) 725
Bpu14I TTCGAA 2 cut(s) 33, 1804
BpuEI CTTGAG 1 cut(s) 1231
Bsa29I ATCGAT 2 cut(s) 1345, 1366
BsaHI GRCGYC 1 cut(s) 865
BsaJI CCNNGG 3 cut(s) 451, 1210, 1520
Bsc4I CCNNNNNNNGG 4 cut(s) 340, 793, 1304, 1407
Bse1I ACTGG 1 cut(s) 1030
Bse3DI GCAATG 1 cut(s) 1237
BseBI CCWGG 1 cut(s) 1257
BseCI ATCGAT 2 cut(s) 1345, 1366
BseDI CCNNGG 3 cut(s) 451, 1210, 1520
BseGI GGATG 5 cut(s) 381, 469, 649, 685, 802
BseLI CCNNNNNNNGG 4 cut(s) 340, 793, 1304, 1407
BseMI GCAATG 1 cut(s) 1237
BseMII CTCAG 4 cut(s) 264, 739, 986, 1158
BseNI ACTGG 1 cut(s) 1030
BseRI GAGGAG 3 cut(s) 230, 290, 641
BseSI GKGCMC 1 cut(s) 1760
BseXI GCAGC 4 cut(s) 802, 955, 1057, 1817
BsgI GTGCAG 1 cut(s) 903
BshFI GGCC 3 cut(s) 786, 1225, 1777
BshVI ATCGAT 2 cut(s) 1345, 1366
BsiHKAI GWGCWC 1 cut(s) 314
BslFI GGGAC 1 cut(s) 925
BslI CCNNNNNNNGG 4 cut(s) 340, 793, 1304, 1407
BsmAI GTCTC 4 cut(s) 285, 1328, 1475, 1493
BsmFI GGGAC 1 cut(s) 925
BsnI GGCC 3 cut(s) 786, 1225, 1777
Bsp119I TTCGAA 2 cut(s) 33, 1804
Bsp1286I GDGCHC 2 cut(s) 314, 1760
Bsp143I GATC 8 cut(s) 37, 94, 186, 346, 754, 904, 1589, 1687
Bsp1720I GCTNAGC 1 cut(s) 725
BspACI CCGC 5 cut(s) 416, 668, 729, 1164, 1207
BspANI GGCC 3 cut(s) 786, 1225, 1777
BspCNI CTCAG 4 cut(s) 265, 738, 985, 1159
BspDI ATCGAT 2 cut(s) 1345, 1366
BspHI TCATGA 1 cut(s) 1119
BspLI GGNNCC 4 cut(s) 111, 228, 408, 1591
BspMAI CTGCAG 3 cut(s) 505, 792, 1075
BspPI GGATC 3 cut(s) 762, 1584, 1597
BspT104I TTCGAA 2 cut(s) 33, 1804
BspTI CTTAAG 1 cut(s) 1300
BsrDI GCAATG 1 cut(s) 1237
BsrI ACTGG 1 cut(s) 1030
BssECI CCNNGG 3 cut(s) 451, 1210, 1520
BssMI GATC 8 cut(s) 37, 94, 186, 346, 754, 904, 1589, 1687
BssNI GRCGYC 1 cut(s) 865
BssT1I CCWWGG 2 cut(s) 1210, 1520
Bst2UI CCWGG 1 cut(s) 1257
Bst4CI ACNGT 6 cut(s) 578, 1014, 1327, 1504, 1517, 1531
Bst6I CTCTTC 2 cut(s) 605, 955
BstACI GRCGYC 1 cut(s) 865
BstAFI CTTAAG 1 cut(s) 1300
BstAPI GCANNNNNTGC 1 cut(s) 799
BstBI TTCGAA 2 cut(s) 33, 1804
BstC8I GCNNGC 4 cut(s) 788, 1021, 1227, 1458
BstDEI CTNAG 5 cut(s) 98, 273, 725, 972, 1167
BstENI CCTNNNNNAGG 1 cut(s) 791
BstF5I GGATG 5 cut(s) 381, 469, 649, 685, 802
BstKTI GATC 8 cut(s) 40, 97, 189, 349, 757, 907, 1592, 1690
BstMAI GTCTC 4 cut(s) 285, 1328, 1475, 1493
BstMBI GATC 8 cut(s) 37, 94, 186, 346, 754, 904, 1589, 1687
BstMWI GCNNNNNNNGC 8 cut(s) 799, 931, 940, 1007, 1016, 1070, 1103, 1172
BstNI CCWGG 1 cut(s) 1257
BstNSI RCATGY 2 cut(s) 244, 587
BstSCI CCNGG 1 cut(s) 1255
BstSFI CTRYAG 4 cut(s) 153, 501, 788, 1071
BstSLI GKGCMC 1 cut(s) 1760
BstV1I GCAGC 4 cut(s) 802, 955, 1057, 1817
BstV2I GAAGAC 2 cut(s) 129, 1258
BstX2I RGATCY 1 cut(s) 1589
BstYI RGATCY 1 cut(s) 1589
Bsu15I ATCGAT 2 cut(s) 1345, 1366
BsuI GTATCC 3 cut(s) 1214, 1519, 1660
BsuRI GGCC 3 cut(s) 786, 1225, 1777
BsuTUI ATCGAT 2 cut(s) 1345, 1366
BtrI CACGTC 1 cut(s) 1727
BtsCI GGATG 5 cut(s) 381, 469, 649, 685, 802
BtsI GCAGTG 1 cut(s) 1799
BtsIMutI CAGTG 4 cut(s) 270, 583, 1023, 1799
Cac8I GCNNGC 4 cut(s) 788, 1021, 1227, 1458
CaiI CAGNNNCTG 1 cut(s) 890
CciI TCATGA 1 cut(s) 1119
Cfr13I GGNCC 3 cut(s) 109, 1262, 1776
ClaI ATCGAT 2 cut(s) 1345, 1366
CseI GACGC 2 cut(s) 854, 1053
CsiI ACCWGGT 1 cut(s) 1255
Csp6I GTAC 3 cut(s) 70, 78, 1486
CviQI GTAC 3 cut(s) 70, 78, 1486
DdeI CTNAG 5 cut(s) 98, 273, 725, 972, 1167
DpnI GATC 8 cut(s) 39, 96, 188, 348, 756, 906, 1591, 1689
DpnII GATC 8 cut(s) 37, 94, 186, 346, 754, 904, 1589, 1687
Eam1104I CTCTTC 2 cut(s) 605, 955
EarI CTCTTC 2 cut(s) 605, 955
EciI GGCGGA 1 cut(s) 1196
Eco130I CCWWGG 2 cut(s) 1210, 1520
Eco32I GATATC 1 cut(s) 1078
Eco47I GGWCC 2 cut(s) 109, 1262
Eco57I CTGAAG 1 cut(s) 950
EcoNI CCTNNNNNAGG 1 cut(s) 791
EcoRII CCWGG 1 cut(s) 1255
EcoRV GATATC 1 cut(s) 1078
EcoT14I CCWWGG 2 cut(s) 1210, 1520
ErhI CCWWGG 2 cut(s) 1210, 1520
FaqI GGGAC 1 cut(s) 925
FauNDI CATATG 2 cut(s) 520, 1149
FbaI TGATCA 2 cut(s) 186, 346
Fnu4HI GCNGC 5 cut(s) 791, 944, 1071, 1164, 1831
FokI GGATG 5 cut(s) 388, 476, 656, 692, 809
Fsp4HI GCNGC 5 cut(s) 791, 944, 1071, 1164, 1831
FspBI CTAG 3 cut(s) 483, 803, 918
GluI GCNGC 5 cut(s) 791, 944, 1071, 1164, 1831
HaeIII GGCC 3 cut(s) 786, 1225, 1777
HgaI GACGC 2 cut(s) 854, 1053
Hin1I GRCGYC 1 cut(s) 865
HinfI GANTC 6 cut(s) 5, 83, 278, 613, 1555, 1801
HphI GGTGA 3 cut(s) 179, 704, 974
Hpy166II GTNNAC 3 cut(s) 1034, 1262, 1513
Hpy8I GTNNAC 3 cut(s) 1034, 1262, 1513
HpyAV CCTTC 5 cut(s) 56, 293, 709, 808, 838
HpyCH4III ACNGT 6 cut(s) 578, 1014, 1327, 1504, 1517, 1531
HpyCH4IV ACGT 1 cut(s) 1726
HpyF10VI GCNNNNNNNGC 8 cut(s) 799, 931, 940, 1007, 1016, 1070, 1103, 1172
HpyF3I CTNAG 5 cut(s) 98, 273, 725, 972, 1167
HpySE526I ACGT 1 cut(s) 1726
Hsp92I GRCGYC 1 cut(s) 865
Ksp22I TGATCA 2 cut(s) 186, 346
Kzo9I GATC 8 cut(s) 37, 94, 186, 346, 754, 904, 1589, 1687
LmnI GCTCC 2 cut(s) 226, 773
Lsp1109I GCAGC 4 cut(s) 802, 955, 1057, 1817
LweI GCATC 3 cut(s) 403, 1351, 1588
MabI ACCWGGT 1 cut(s) 1255
MaeI CTAG 3 cut(s) 483, 803, 918
MaeII ACGT 1 cut(s) 1726
MaeIII GTNAC 1 cut(s) 556
MalI GATC 8 cut(s) 39, 96, 188, 348, 756, 906, 1591, 1689
MboI GATC 8 cut(s) 37, 94, 186, 346, 754, 904, 1589, 1687
MflI RGATCY 1 cut(s) 1589
MhlI GDGCHC 2 cut(s) 314, 1760
MluCI AATT 8 cut(s) 28, 431, 443, 936, 1271, 1465, 1537, 1767
MlyI GAGTC 3 cut(s) 77, 287, 622
MmeI TCCRAC 2 cut(s) 1444, 1644
MroXI GAANNNNTTC 1 cut(s) 1182
MseI TTAA 9 cut(s) 447, 654, 1301, 1383, 1434, 1440, 1464, 1482, 1584
MslI CAYNNNNRTG 2 cut(s) 588, 1124
MspA1I CMGCKG 4 cut(s) 729, 1070, 1166, 1830
MspCI CTTAAG 1 cut(s) 1300
MspR9I CCNGG 1 cut(s) 1257
MvaI CCWGG 1 cut(s) 1257
MwoI GCNNNNNNNGC 8 cut(s) 799, 931, 940, 1007, 1016, 1070, 1103, 1172
NdeI CATATG 2 cut(s) 520, 1149
NdeII GATC 8 cut(s) 37, 94, 186, 346, 754, 904, 1589, 1687
NlaIV GGNNCC 4 cut(s) 111, 228, 408, 1591
NspI RCATGY 2 cut(s) 244, 587
NspV TTCGAA 2 cut(s) 33, 1804
PagI TCATGA 1 cut(s) 1119
PdmI GAANNNNTTC 1 cut(s) 1182
PfeI GAWTC 3 cut(s) 5, 1555, 1801
PflMI CCANNNNNTGG 1 cut(s) 1407
PkrI GCNGC 5 cut(s) 792, 945, 1072, 1165, 1832
PleI GAGTC 3 cut(s) 77, 286, 621
PpsI GAGTC 3 cut(s) 77, 286, 621
PsiI TTATAA 1 cut(s) 1157
Psp6I CCWGG 1 cut(s) 1255
PspGI CCWGG 1 cut(s) 1255
PspN4I GGNNCC 4 cut(s) 111, 228, 408, 1591
PspPI GGNCC 3 cut(s) 109, 1262, 1776
PstI CTGCAG 3 cut(s) 505, 792, 1075
PstNI CAGNNNCTG 1 cut(s) 890
PsuI RGATCY 1 cut(s) 1589
PvuII CAGCTG 2 cut(s) 1070, 1830
RsaI GTAC 3 cut(s) 71, 79, 1487
RsaNI GTAC 3 cut(s) 70, 78, 1486
RseI CAYNNNNRTG 2 cut(s) 588, 1124
SaqAI TTAA 9 cut(s) 447, 654, 1301, 1383, 1434, 1440, 1464, 1482, 1584
SatI GCNGC 5 cut(s) 791, 944, 1071, 1164, 1831
Sau3AI GATC 8 cut(s) 37, 94, 186, 346, 754, 904, 1589, 1687
Sau96I GGNCC 3 cut(s) 109, 1262, 1776
ScaI AGTACT 1 cut(s) 79
SchI GAGTC 3 cut(s) 77, 287, 622
ScrFI CCNGG 1 cut(s) 1257
SduI GDGCHC 2 cut(s) 314, 1760
SexAI ACCWGGT 1 cut(s) 1255
SfaNI GCATC 3 cut(s) 403, 1351, 1588
SfcI CTRYAG 4 cut(s) 153, 501, 788, 1071
SfuI TTCGAA 2 cut(s) 33, 1804
SinI GGWCC 2 cut(s) 109, 1262
SmiMI CAYNNNNRTG 2 cut(s) 588, 1124
SmlI CTYRAG 2 cut(s) 1246, 1300
SmoI CTYRAG 2 cut(s) 1246, 1300
Sse9I AATT 8 cut(s) 28, 431, 443, 936, 1271, 1465, 1537, 1767
SsiI CCGC 5 cut(s) 416, 668, 729, 1164, 1207
SspMI CTAG 3 cut(s) 483, 803, 918
StyD4I CCNGG 1 cut(s) 1255
StyI CCWWGG 2 cut(s) 1210, 1520
TaaI ACNGT 6 cut(s) 578, 1014, 1327, 1504, 1517, 1531
TaiI ACGT 1 cut(s) 1729
TasI AATT 8 cut(s) 28, 431, 443, 936, 1271, 1465, 1537, 1767
TatI WGTACW 1 cut(s) 77
TauI GCSGC 1 cut(s) 1166
TfiI GAWTC 3 cut(s) 5, 1555, 1801
Tru1I TTAA 9 cut(s) 447, 654, 1301, 1383, 1434, 1440, 1464, 1482, 1584
Tru9I TTAA 9 cut(s) 447, 654, 1301, 1383, 1434, 1440, 1464, 1482, 1584
TscAI CASTG 4 cut(s) 277, 583, 1030, 1799
TseI GCWGC 4 cut(s) 790, 943, 1070, 1830
TspDTI ATGAA 8 cut(s) 137, 578, 842, 867, 1108, 1136, 1175, 1226
TspRI CASTG 4 cut(s) 277, 583, 1030, 1799
Van91I CCANNNNNTGG 1 cut(s) 1407
Vha464I CTTAAG 1 cut(s) 1300
VpaK11BI GGWCC 2 cut(s) 109, 1262
XagI CCTNNNNNAGG 1 cut(s) 791
XapI RAATTY 1 cut(s) 1537
XceI RCATGY 2 cut(s) 244, 587
XmnI GAANNNNTTC 1 cut(s) 1182
XspI CTAG 3 cut(s) 483, 803, 918
ZrmI AGTACT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.