Rmu_co8409941.1_g000001

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8409941.1
OG0000624
Physical Location & Seq
Reverse (-)
274 .. 1323
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8409941.1_g000001.1.cds

Sequence Viewer

Length: 1050 bp
atgcagcactcttccccatttggtgattgtgctcagaggttggctcattgctttgcaaatgctcttgaagcgcggttagctggcactggaactcaaatatatactgctttatgtgctaaacgaatgtcagctgctgatatgctgaagttttatcaagcttatattgcagcctgcccattcatgaagatgtccatgatctttgcaaaccatatgattttaaaagcagctgagaaagcagaaacacttcatataatagattttggtatcctttatggttttcagtggcctgctctcattcattgcctttcaagaagacctggtggaccgcccaaattacgtattacaggggtagagctccccttaagtggttttcgaccagaagaaagagtccaagagacagggcatcgcttggctaagtattgtaagcgatttaatgttccttttgagtactttgccatagccaaaaagtgggaaactgtccaatttgatgacctcaaagttcaaagaaatgaggtgcttgctgttaattgtctctgccgatttaagaacctccttgatgagaccgttgtggtaaaaagtccaagggatactgttctaaatttaattaggaagttaaatcctgatgtgtttgtccaaagtgttgttaatggatcctatcatgcacccttctttgtttcacgcttccgagaggcactattccacttctctgcattgtttgatatgtttgataaaaatttgcctcgtgaagatccgatgagattagggtttgagaaagagtttattggacgtgaagttgtgaatatagtagcttgtgagggctcagaaagaactgtgaggccagaaacatataagcagtggcaggttcggaacataagggctgggttcagacagctgcccttggactgtgaagtcatgaacaaaataaaggataaagtaaaaacggggtacagccctgattttgtggttgatgaggatggccgctggatgctgcagggatggaagggcagaattatgtttggttccgcctgttgggtaccgtctaggacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

349

Amino Acids

40.14

Weight (kDa)

9.2

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 908
Acc36I ACCTGC 1 cut(s) 850
Acc65I GGTACC 1 cut(s) 1033
AccB1I GGYRCC 1 cut(s) 1033
AccB7I CCANNNNNTGG 1 cut(s) 468
AccII CGCG 1 cut(s) 73
AciI CCGC 4 cut(s) 73, 326, 979, 1023
AclWI GGATC 3 cut(s) 645, 658, 743
AcoI YGGCCR 1 cut(s) 976
AcsI RAATTY 2 cut(s) 598, 733
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 3 cut(s) 449, 947, 1035
AfiI CCNNNNNNNGG 3 cut(s) 365, 468, 1029
AflII CTTAAG 1 cut(s) 361
AgsI TTSAA 3 cut(s) 68, 309, 503
AjiI CACGTC 1 cut(s) 788
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 7 cut(s) 80, 131, 158, 227, 355, 809, 892
AluI AGCT 7 cut(s) 80, 131, 158, 227, 355, 809, 892
Alw21I GWGCWC 2 cut(s) 34, 357
Alw26I GTCTC 3 cut(s) 389, 536, 554
AlwI GGATC 3 cut(s) 645, 658, 743
AlwNI CAGNNNCTG 1 cut(s) 134
AoxI GGCC 3 cut(s) 284, 836, 976
ApeKI GCWGC 6 cut(s) 4, 131, 167, 224, 892, 988
ApoI RAATTY 2 cut(s) 598, 733
Asp700I GAANNNNTTC 1 cut(s) 243
Asp718I GGTACC 1 cut(s) 1033
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 35
AvaII GGWCC 1 cut(s) 323
BamHI GGATCC 1 cut(s) 650
BanI GGYRCC 1 cut(s) 1033
BanII GRGCYC 2 cut(s) 357, 821
BauI CACGAG 1 cut(s) 741
BbsI GAAGAC 1 cut(s) 319
Bbv12I GWGCWC 2 cut(s) 34, 357
BbvI GCAGC 6 cut(s) 16, 118, 179, 236, 879, 975
BccI CCATC 2 cut(s) 968, 990
BciT130I CCWGG 1 cut(s) 318
BciVI GTATCC 2 cut(s) 275, 580
BcoDI GTCTC 3 cut(s) 389, 536, 554
BfaI CTAG 1 cut(s) 1041
BfmI CTRYAG 1 cut(s) 989
BfrI CTTAAG 1 cut(s) 361
BfuAI ACCTGC 1 cut(s) 850
BfuI GTATCC 2 cut(s) 275, 580
BisI GCNGC 7 cut(s) 5, 132, 168, 225, 893, 979, 989
BlsI GCNGC 7 cut(s) 6, 133, 169, 226, 894, 980, 990
BmcAI AGTACT 1 cut(s) 449
Bme1390I CCNGG 1 cut(s) 318
Bme18I GGWCC 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 788
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 3 cut(s) 652, 1021, 1035
BmrFI CCNGG 1 cut(s) 318
BmsI GCATC 2 cut(s) 412, 975
BpiI GAAGAC 1 cut(s) 319
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BsaAI YACGTR 1 cut(s) 338
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 2 cut(s) 581, 897
Bsc4I CCNNNNNNNGG 3 cut(s) 365, 468, 1029
Bse1I ACTGG 1 cut(s) 91
Bse3DI GCAATG 2 cut(s) 46, 298
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 2 cut(s) 581, 897
BseGI GGATG 3 cut(s) 979, 990, 1001
BseLI CCNNNNNNNGG 3 cut(s) 365, 468, 1029
BseMI GCAATG 2 cut(s) 46, 298
BseMII CTCAG 3 cut(s) 47, 219, 834
BseNI ACTGG 1 cut(s) 91
BseXI GCAGC 6 cut(s) 16, 118, 179, 236, 879, 975
BseYI CCCAGC 1 cut(s) 878
Bsh1236I CGCG 1 cut(s) 73
BshFI GGCC 3 cut(s) 286, 838, 978
BshNI GGYRCC 1 cut(s) 1033
BsiHKAI GWGCWC 2 cut(s) 34, 357
BslI CCNNNNNNNGG 3 cut(s) 365, 468, 1029
BsmAI GTCTC 3 cut(s) 389, 536, 554
BsnI GGCC 3 cut(s) 286, 838, 978
Bso31I GGTCTC 1 cut(s) 554
Bsp1286I GDGCHC 3 cut(s) 34, 357, 821
Bsp143I GATC 3 cut(s) 195, 650, 748
BspACI CCGC 4 cut(s) 73, 326, 979, 1023
BspANI GGCC 3 cut(s) 286, 838, 978
BspCNI CTCAG 3 cut(s) 46, 220, 833
BspFNI CGCG 1 cut(s) 73
BspHI TCATGA 2 cut(s) 180, 912
BspLI GGNNCC 3 cut(s) 652, 1021, 1035
BspMAI CTGCAG 1 cut(s) 993
BspMI ACCTGC 1 cut(s) 850
BspPI GGATC 3 cut(s) 645, 658, 743
BspT107I GGYRCC 1 cut(s) 1033
BspTI CTTAAG 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 554
BsrDI GCAATG 2 cut(s) 46, 298
BsrI ACTGG 1 cut(s) 91
BssECI CCNNGG 2 cut(s) 581, 897
BssMI GATC 3 cut(s) 195, 650, 748
BssSI CACGAG 1 cut(s) 741
BssT1I CCWWGG 2 cut(s) 581, 897
Bst2BI CACGAG 1 cut(s) 741
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 6 cut(s) 478, 565, 592, 832, 905, 1038
Bst6I CTCTTC 1 cut(s) 16
BstAFI CTTAAG 1 cut(s) 361
BstBAI YACGTR 1 cut(s) 338
BstC8I GCNNGC 4 cut(s) 82, 172, 288, 519
BstDEI CTNAG 4 cut(s) 33, 228, 414, 820
BstF5I GGATG 3 cut(s) 979, 990, 1001
BstFNI CGCG 1 cut(s) 73
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 3 cut(s) 198, 653, 751
BstMAI GTCTC 3 cut(s) 389, 536, 554
BstMBI GATC 3 cut(s) 195, 650, 748
BstMWI GCNNNNNNNGC 5 cut(s) 68, 77, 113, 164, 233
BstNI CCWGG 1 cut(s) 318
BstSCI CCNGG 1 cut(s) 316
BstSFI CTRYAG 1 cut(s) 989
BstSNI TACGTA 1 cut(s) 338
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 6 cut(s) 16, 118, 179, 236, 879, 975
BstV2I GAAGAC 1 cut(s) 319
BstX2I RGATCY 2 cut(s) 650, 748
BstYI RGATCY 2 cut(s) 650, 748
BsuI GTATCC 2 cut(s) 275, 580
BsuRI GGCC 3 cut(s) 286, 838, 978
BtgZI GCGATG 1 cut(s) 389
BtrI CACGTC 1 cut(s) 788
BtsCI GGATG 3 cut(s) 979, 990, 1001
BtsI GCAGTG 1 cut(s) 860
BtsIMutI CAGTG 3 cut(s) 84, 287, 860
BveI ACCTGC 1 cut(s) 850
Cac8I GCNNGC 4 cut(s) 82, 172, 288, 519
CaiI CAGNNNCTG 1 cut(s) 134
CciI TCATGA 2 cut(s) 180, 912
CfoI GCGC 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 323
CsiI ACCWGGT 1 cut(s) 316
Csp6I GTAC 3 cut(s) 448, 946, 1034
CviAII CATG 4 cut(s) 181, 193, 659, 913
CviQI GTAC 3 cut(s) 448, 946, 1034
DdeI CTNAG 4 cut(s) 33, 228, 414, 820
DpnI GATC 3 cut(s) 197, 652, 750
DpnII GATC 3 cut(s) 195, 650, 748
DraI TTTAAA 1 cut(s) 219
DrdI GACNNNNNNGTC 1 cut(s) 908
DseDI GACNNNNNNGTC 1 cut(s) 908
EaeI YGGCCR 1 cut(s) 976
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
EciI GGCGGA 1 cut(s) 1012
Ecl136II GAGCTC 1 cut(s) 355
Eco105I TACGTA 1 cut(s) 338
Eco130I CCWWGG 2 cut(s) 581, 897
Eco24I GRGCYC 2 cut(s) 357, 821
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 323
Eco53kI GAGCTC 1 cut(s) 355
Eco57I CTGAAG 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 316
EcoT14I CCWWGG 2 cut(s) 581, 897
EcoT38I GRGCYC 2 cut(s) 357, 821
ErhI CCWWGG 2 cut(s) 581, 897
FaeI CATG 4 cut(s) 184, 196, 662, 916
FatI CATG 4 cut(s) 180, 192, 658, 912
FauNDI CATATG 1 cut(s) 210
Fnu4HI GCNGC 7 cut(s) 5, 132, 168, 225, 893, 979, 989
FokI GGATG 3 cut(s) 986, 997, 1008
FriOI GRGCYC 2 cut(s) 357, 821
Fsp4HI GCNGC 7 cut(s) 5, 132, 168, 225, 893, 979, 989
FspBI CTAG 1 cut(s) 1041
GlaI GCGC 1 cut(s) 72
GluI GCNGC 7 cut(s) 5, 132, 168, 225, 893, 979, 989
GsaI CCCAGC 1 cut(s) 882
HaeIII GGCC 3 cut(s) 286, 838, 978
HhaI GCGC 1 cut(s) 73
Hin1II CATG 4 cut(s) 184, 196, 662, 916
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HindIII AAGCTT 1 cut(s) 156
HinfI GANTC 1 cut(s) 387
HphI GGTGA 1 cut(s) 35
Hpy166II GTNNAC 1 cut(s) 323
Hpy188I TCNGA 6 cut(s) 36, 686, 753, 823, 867, 887
Hpy188III TCNNGA 6 cut(s) 65, 181, 309, 620, 743, 913
Hpy8I GTNNAC 1 cut(s) 323
HpyAV CCTTC 2 cut(s) 676, 994
HpyCH4III ACNGT 6 cut(s) 478, 565, 592, 832, 905, 1038
HpyCH4IV ACGT 2 cut(s) 337, 787
HpyCH4V TGCA 7 cut(s) 4, 56, 167, 203, 662, 710, 991
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 77, 113, 164, 233
HpyF3I CTNAG 4 cut(s) 33, 228, 414, 820
HpySE526I ACGT 2 cut(s) 337, 787
Hsp92II CATG 4 cut(s) 184, 196, 662, 916
HspAI GCGC 1 cut(s) 71
KpnI GGTACC 1 cut(s) 1037
Kzo9I GATC 3 cut(s) 195, 650, 748
LmnI GCTCC 1 cut(s) 360
Lsp1109I GCAGC 6 cut(s) 16, 118, 179, 236, 879, 975
LweI GCATC 2 cut(s) 412, 975
MabI ACCWGGT 1 cut(s) 316
MaeI CTAG 1 cut(s) 1041
MaeII ACGT 2 cut(s) 337, 787
MalI GATC 3 cut(s) 197, 652, 750
MboI GATC 3 cut(s) 195, 650, 748
MboII GAAGA 5 cut(s) 3, 196, 324, 392, 758
MflI RGATCY 2 cut(s) 650, 748
MhlI GDGCHC 3 cut(s) 34, 357, 821
MluCI AATT 7 cut(s) 332, 482, 526, 598, 603, 733, 1008
MlyI GAGTC 1 cut(s) 396
MnlI CCTC 9 cut(s) 30, 503, 505, 560, 682, 750, 808, 828, 964
MroXI GAANNNNTTC 1 cut(s) 243
MseI TTAA 8 cut(s) 218, 362, 432, 525, 543, 602, 614, 645
MslI CAYNNNNRTG 1 cut(s) 185
MspA1I CMGCKG 4 cut(s) 131, 227, 892, 981
MspCI CTTAAG 1 cut(s) 361
MspR9I CCNGG 1 cut(s) 318
MvaI CCWGG 1 cut(s) 318
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 5 cut(s) 68, 77, 113, 164, 233
NdeI CATATG 1 cut(s) 210
NdeII GATC 3 cut(s) 195, 650, 748
NlaIII CATG 4 cut(s) 184, 196, 662, 916
NlaIV GGNNCC 3 cut(s) 652, 1021, 1035
PagI TCATGA 2 cut(s) 180, 912
PdmI GAANNNNTTC 1 cut(s) 243
PflMI CCANNNNNTGG 1 cut(s) 468
PkrI GCNGC 7 cut(s) 6, 133, 169, 226, 894, 980, 990
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
Ppu21I YACGTR 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 316
PspFI CCCAGC 1 cut(s) 878
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 3 cut(s) 652, 1021, 1035
PspPI GGNCC 1 cut(s) 323
PstI CTGCAG 1 cut(s) 993
PstNI CAGNNNCTG 1 cut(s) 134
PsuI RGATCY 2 cut(s) 650, 748
PvuII CAGCTG 3 cut(s) 131, 227, 892
RsaI GTAC 3 cut(s) 449, 947, 1035
RsaNI GTAC 3 cut(s) 448, 946, 1034
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 1 cut(s) 357
SaqAI TTAA 8 cut(s) 218, 362, 432, 525, 543, 602, 614, 645
SatI GCNGC 7 cut(s) 5, 132, 168, 225, 893, 979, 989
Sau3AI GATC 3 cut(s) 195, 650, 748
Sau96I GGNCC 1 cut(s) 323
ScaI AGTACT 1 cut(s) 449
SchI GAGTC 1 cut(s) 396
ScrFI CCNGG 1 cut(s) 318
SduI GDGCHC 3 cut(s) 34, 357, 821
SexAI ACCWGGT 1 cut(s) 316
SfaNI GCATC 2 cut(s) 412, 975
SfcI CTRYAG 1 cut(s) 989
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
SnaBI TACGTA 1 cut(s) 338
Sse9I AATT 7 cut(s) 332, 482, 526, 598, 603, 733, 1008
SsiI CCGC 4 cut(s) 73, 326, 979, 1023
SspMI CTAG 1 cut(s) 1041
SstI GAGCTC 1 cut(s) 357
StyD4I CCNGG 1 cut(s) 316
StyI CCWWGG 2 cut(s) 581, 897
TaaI ACNGT 6 cut(s) 478, 565, 592, 832, 905, 1038
TaiI ACGT 2 cut(s) 340, 790
TaqI TCGA 1 cut(s) 373
TasI AATT 7 cut(s) 332, 482, 526, 598, 603, 733, 1008
TatI WGTACW 1 cut(s) 447
TauI GCSGC 1 cut(s) 981
Tru1I TTAA 8 cut(s) 218, 362, 432, 525, 543, 602, 614, 645
Tru9I TTAA 8 cut(s) 218, 362, 432, 525, 543, 602, 614, 645
TscAI CASTG 3 cut(s) 91, 287, 860
TseI GCWGC 6 cut(s) 4, 131, 167, 224, 892, 988
TspDTI ATGAA 5 cut(s) 169, 197, 236, 287, 929
TspRI CASTG 3 cut(s) 91, 287, 860
Van91I CCANNNNNTGG 1 cut(s) 468
Vha464I CTTAAG 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 2 cut(s) 598, 733
XmnI GAANNNNTTC 1 cut(s) 243
XspI CTAG 1 cut(s) 1041
ZrmI AGTACT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.