Rh5DG457900

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000624
Physical Location & Seq
Forward (+)
72805146 .. 72807592
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG457900.1

Sequence Viewer

Length: 1758 bp
ATGTTCCATGACCCTTTGGCTCTTCAAGCCACTGTGGAGTCTCTTCATGAGGTTATTGGAGGAGGGAAGTACCCTACTTCCCAAGACCTAAACCCTCTTTCTGTTGATCAAGATGTGTATGGAAATGGAAATGGGATGGCGGGTTCTTATAGGAGTGAGCTAATGGTTCCTGATGTGCTTAGTAAGAATGAATTGTTATTGCAATTTAACCTAGGGGCAGAGGAGGGTAGTAAGTTTCTTCCTAGAGGTCAGTTGATTAGTGCAGAGGGCAACAAGCCGTATGCTGTGGCGGATAGAAAGGCTGGAGATGTGGCAGTTACGACCCAGAAGAATGGGAGTGAGCGTTTTCGTGTTGGTTCTAGAGGAAAGAAAAATCATCAAAGGGAAGATATAGATTTGGAGGATGGGAGGAGTAACAAGCAATCGGCGGTTTATATGGATGGTGAGGAGGGTGAGCTGTCTGATATTTTTGACAAGGTGTTGCTCTCCAAGCCTATCAAGAGTAAAGACGATCAAGTCAGTCAGAATGGAGCAAACAATAGCCTGCAGCAGGATGGGCTATCGTTTGGAATCGGTAATGGGAAGGTTTGCGGCAAGAATCAAGGCAACAAGAAGGAAGTGATAGATTTGAGGGCTCTTCTGATTTCATGTGCAGAAGCTGTATCTGTTGATGACCGCGAGACTGCTAGTGAGCTACTAAAGCAGATTATGCAGCACTCTTCCCCATTTGGTGACTGTACTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTGAAGCGCGGTTGGCTGGCACAGGAACTCAAATATATACTGCTTTAACTACAAAACGAATGTCAGCTGCTGATTCGCTGAAGTTTTACCAAGCTTATATTGCAGTCTGCCCGTTCATGAAGCTGTTGATGATCTTTGCAAACAATATGATTTTAAAAGCTGCTGAGAAAGCAGAAACACTACATATAATAGATTTTGGTATCCGTTATGGTTTTCAATGGCCTGCTCTCATTCATTGCCTCTCAAGAAGACCTGGTGGACCTCCCAAATTACGCATTACAGGGTTAGAGTTTCCCTCAAGTGGTTTTCGACCAGAAGAAGGAGTCCAAGAGACAGGGCATCGCTTGGCAAAGTATTGTAGGCGATTTAATGTTCATTTTGAGTACCATGGCATAGCCAAAAAATGGGAAACTCTCCAAGTTGATGACCTTAAAGTTCAAAGAAATGAGGTGCTTGCAGTTAATTGTCTCAACCGATTCAAGAACCTCGTTGATGAGACTGTTGTGGTAAACAGTCCAAGGGATACTGTTCTAAATTTAATTAGGAATTTGAATCCTGATGTCTTTGTCCAAAGTGTTGTTAATGGATGCCATCATGCACCCTTCTTTGTTCCACGCTTCCAAGAGGCACTATTCCACTTCTCTGCATTGTTTGATATGTTGGATACCAATTTGCCTCGTGAAGATCAGATGAGATTGGGGTTTGAGAAAGAGTTTCTTGGACGTGAAGTTGTGAATATAGTAGCTTGTGAGGGCTTTCAAAGAATTGTTAGGCCAGAAACATACAAGCAGTGGCAGATTCGAAACATGAGGGCTGGGTTCAGACAGCTGCCATTGGACTGTGAAGTCATGAACAAAATAAAGGATAAGGTAAAAATGGGGTACCACCCTTCTTTTGTGGTTTATGAAGATGGCCGCTGGATGCTGCATGAATGGAAGGGCAGAATTATGTATGCTTCTGCCTGTTGGGCACCATCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

585

Amino Acids

65.87

Weight (kDa)

7.91

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 210 - 580 4.5e-117 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 515, 1616
Acc65I GGTACC 1 cut(s) 1653
AccB1I GGYRCC 2 cut(s) 1653, 1741
AccB7I CCANNNNNTGG 1 cut(s) 1176
AccII CGCG 2 cut(s) 678, 781
AciI CCGC 7 cut(s) 140, 290, 428, 591, 676, 781, 1687
AcoI YGGCCR 1 cut(s) 1684
AcsI RAATTY 2 cut(s) 1306, 1318
AcuI CTGAAG 1 cut(s) 872
AfaI GTAC 4 cut(s) 71, 739, 1157, 1655
AfiI CCNNNNNNNGG 4 cut(s) 550, 1073, 1091, 1176
AgsI TTSAA 7 cut(s) 26, 776, 989, 1211, 1252, 1324, 1532
AjiI CACGTC 1 cut(s) 1496
AjnI CCWGG 1 cut(s) 1024
AloI GAACNNNNNNTCC 2 cut(s) 127, 159
Alw26I GTCTC 5 cut(s) 45, 674, 1097, 1244, 1262
AlwNI CAGNNNCTG 2 cut(s) 659, 842
AoxI GGCC 3 cut(s) 992, 1544, 1684
ApeKI GCWGC 6 cut(s) 547, 712, 839, 932, 1600, 1696
ApoI RAATTY 2 cut(s) 1306, 1318
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
Asp718I GGTACC 1 cut(s) 1653
AspA2I CCTAGG 1 cut(s) 211
AspLEI GCGC 1 cut(s) 781
AspS9I GGNCC 1 cut(s) 1031
AsuHPI GGTGA 3 cut(s) 455, 464, 743
AsuII TTCGAA 1 cut(s) 1573
AvaII GGWCC 1 cut(s) 1031
AvrII CCTAGG 1 cut(s) 211
BaeGI GKGCMC 1 cut(s) 1744
BanI GGYRCC 2 cut(s) 1653, 1741
BanII GRGCYC 1 cut(s) 637
BauI CACGAG 1 cut(s) 1449
BbsI GAAGAC 1 cut(s) 1027
BbvI GCAGC 6 cut(s) 559, 724, 826, 919, 1587, 1683
BccI CCATC 7 cut(s) 130, 398, 434, 548, 1371, 1676, 1753
BceAI ACGGC 1 cut(s) 262
BciT130I CCWGG 1 cut(s) 1026
BciVI GTATCC 3 cut(s) 983, 1288, 1429
BclI TGATCA 1 cut(s) 106
BcoDI GTCTC 5 cut(s) 45, 674, 1097, 1244, 1262
BfaI CTAG 5 cut(s) 212, 243, 360, 687, 1749
BfmI CTRYAG 1 cut(s) 545
BfuI GTATCC 3 cut(s) 983, 1288, 1429
BglI GCCNNNNNGGC 1 cut(s) 1739
BisI GCNGC 8 cut(s) 548, 592, 713, 840, 933, 1601, 1687, 1697
BlnI CCTAGG 1 cut(s) 211
BlsI GCNGC 8 cut(s) 549, 593, 714, 841, 934, 1602, 1688, 1698
Bme1390I CCNGG 1 cut(s) 1026
Bme18I GGWCC 1 cut(s) 1031
BmgBI CACGTC 1 cut(s) 1496
BmgT120I GGNCC 1 cut(s) 1031
BmiI GGNNCC 3 cut(s) 168, 1655, 1743
BmrFI CCNGG 1 cut(s) 1026
BmsI GCATC 3 cut(s) 1120, 1349, 1683
BpiI GAAGAC 1 cut(s) 1027
BplI GAGNNNNNCTC 4 cut(s) 736, 768, 1052, 1084
BpmI CTGGAG 1 cut(s) 324
Bpu14I TTCGAA 1 cut(s) 1573
BpuEI CTTGAG 2 cut(s) 1000, 1054
BsaJI CCNNGG 3 cut(s) 211, 1159, 1289
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 4 cut(s) 550, 1073, 1091, 1176
Bse3DI GCAATG 2 cut(s) 754, 1006
BseBI CCWGG 1 cut(s) 1026
BseDI CCNNGG 3 cut(s) 211, 1159, 1289
BseGI GGATG 6 cut(s) 141, 409, 445, 559, 1364, 1698
BseLI CCNNNNNNNGG 4 cut(s) 550, 1073, 1091, 1176
BseMI GCAATG 2 cut(s) 754, 1006
BseMII CTCAG 2 cut(s) 755, 927
BseRI GAGGAG 4 cut(s) 75, 236, 424, 461
BseSI GKGCMC 1 cut(s) 1744
BseXI GCAGC 6 cut(s) 559, 724, 826, 919, 1587, 1683
BseYI CCCAGC 1 cut(s) 1586
BsgI GTGCAG 2 cut(s) 282, 672
Bsh1236I CGCG 2 cut(s) 678, 781
BshFI GGCC 3 cut(s) 994, 1546, 1686
BshNI GGYRCC 2 cut(s) 1653, 1741
BslI CCNNNNNNNGG 4 cut(s) 550, 1073, 1091, 1176
BsmAI GTCTC 5 cut(s) 45, 674, 1097, 1244, 1262
BsnI GGCC 3 cut(s) 994, 1546, 1686
Bsp119I TTCGAA 1 cut(s) 1573
Bsp1286I GDGCHC 2 cut(s) 637, 1744
Bsp143I GATC 4 cut(s) 106, 511, 903, 1456
Bsp19I CCATGG 1 cut(s) 1159
BspACI CCGC 7 cut(s) 140, 290, 428, 591, 676, 781, 1687
BspANI GGCC 3 cut(s) 994, 1546, 1686
BspCNI CTCAG 2 cut(s) 754, 928
BspFNI CGCG 2 cut(s) 678, 781
BspHI TCATGA 3 cut(s) 46, 888, 1620
BspLI GGNNCC 3 cut(s) 168, 1655, 1743
BspMAI CTGCAG 1 cut(s) 549
BspQI GCTCTTC 2 cut(s) 27, 642
BspT104I TTCGAA 1 cut(s) 1573
BspT107I GGYRCC 2 cut(s) 1653, 1741
BsrDI GCAATG 2 cut(s) 754, 1006
BssECI CCNNGG 3 cut(s) 211, 1159, 1289
BssMI GATC 4 cut(s) 106, 511, 903, 1456
BssSI CACGAG 1 cut(s) 1449
BssT1I CCWWGG 3 cut(s) 211, 1159, 1289
Bst2BI CACGAG 1 cut(s) 1449
Bst2UI CCWGG 1 cut(s) 1026
Bst4CI ACNGT 6 cut(s) 34, 737, 1273, 1286, 1300, 1613
Bst6I CTCTTC 4 cut(s) 27, 48, 642, 724
BstAPI GCANNNNNTGC 1 cut(s) 709
BstBI TTCGAA 1 cut(s) 1573
BstC8I GCNNGC 4 cut(s) 545, 790, 996, 1227
BstDEI CTNAG 3 cut(s) 179, 741, 936
BstDSI CCRYGG 1 cut(s) 1159
BstENI CCTNNNNNAGG 1 cut(s) 548
BstF5I GGATG 6 cut(s) 141, 409, 445, 559, 1364, 1698
BstFNI CGCG 2 cut(s) 678, 781
BstHHI GCGC 1 cut(s) 781
BstKTI GATC 4 cut(s) 109, 514, 906, 1459
BstMAI GTCTC 5 cut(s) 45, 674, 1097, 1244, 1262
BstMBI GATC 4 cut(s) 106, 511, 903, 1456
BstNI CCWGG 1 cut(s) 1026
BstSCI CCNGG 1 cut(s) 1024
BstSFI CTRYAG 1 cut(s) 545
BstSLI GKGCMC 1 cut(s) 1744
BstUI CGCG 2 cut(s) 678, 781
BstV1I GCAGC 6 cut(s) 559, 724, 826, 919, 1587, 1683
BstV2I GAAGAC 1 cut(s) 1027
BstXI CCANNNNNNTGG 1 cut(s) 332
BsuI GTATCC 3 cut(s) 983, 1288, 1429
BsuRI GGCC 3 cut(s) 994, 1546, 1686
BtgI CCRYGG 1 cut(s) 1159
BtgZI GCGATG 1 cut(s) 1097
BtrI CACGTC 1 cut(s) 1496
BtsCI GGATG 6 cut(s) 141, 409, 445, 559, 1364, 1698
BtsI GCAGTG 1 cut(s) 1568
BtsIMutI CAGTG 2 cut(s) 30, 1568
Cac8I GCNNGC 4 cut(s) 545, 790, 996, 1227
CaiI CAGNNNCTG 2 cut(s) 659, 842
CciI TCATGA 3 cut(s) 46, 888, 1620
CfoI GCGC 1 cut(s) 781
Cfr13I GGNCC 1 cut(s) 1031
CsiI ACCWGGT 1 cut(s) 1024
Csp6I GTAC 4 cut(s) 70, 738, 1156, 1654
CviAII CATG 9 cut(s) 8, 47, 648, 889, 1160, 1367, 1579, 1621, 1700
CviQI GTAC 4 cut(s) 70, 738, 1156, 1654
DdeI CTNAG 3 cut(s) 179, 741, 936
DpnI GATC 4 cut(s) 108, 513, 905, 1458
DpnII GATC 4 cut(s) 106, 511, 903, 1456
DraI TTTAAA 1 cut(s) 927
DrdI GACNNNNNNGTC 2 cut(s) 515, 1616
DseDI GACNNNNNNGTC 2 cut(s) 515, 1616
EaeI YGGCCR 1 cut(s) 1684
Eam1104I CTCTTC 4 cut(s) 27, 48, 642, 724
EarI CTCTTC 4 cut(s) 27, 48, 642, 724
EciI GGCGGA 1 cut(s) 305
Eco130I CCWWGG 3 cut(s) 211, 1159, 1289
Eco24I GRGCYC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 1031
Eco57I CTGAAG 1 cut(s) 872
EcoNI CCTNNNNNAGG 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 1024
EcoT14I CCWWGG 3 cut(s) 211, 1159, 1289
EcoT38I GRGCYC 1 cut(s) 637
ErhI CCWWGG 3 cut(s) 211, 1159, 1289
FaeI CATG 9 cut(s) 11, 50, 651, 892, 1163, 1370, 1582, 1624, 1703
FalI AAGNNNNNCTT 2 cut(s) 1473, 1505
FatI CATG 9 cut(s) 7, 46, 647, 888, 1159, 1366, 1578, 1620, 1699
FauI CCCGC 1 cut(s) 133
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 8 cut(s) 548, 592, 713, 840, 933, 1601, 1687, 1697
FokI GGATG 6 cut(s) 148, 416, 452, 566, 1371, 1705
FriOI GRGCYC 1 cut(s) 637
Fsp4HI GCNGC 8 cut(s) 548, 592, 713, 840, 933, 1601, 1687, 1697
FspBI CTAG 5 cut(s) 212, 243, 360, 687, 1749
GlaI GCGC 1 cut(s) 780
GluI GCNGC 8 cut(s) 548, 592, 713, 840, 933, 1601, 1687, 1697
GsaI CCCAGC 1 cut(s) 1590
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 3 cut(s) 994, 1546, 1686
HhaI GCGC 1 cut(s) 781
Hin1II CATG 9 cut(s) 11, 50, 651, 892, 1163, 1370, 1582, 1624, 1703
Hin6I GCGC 1 cut(s) 779
HinP1I GCGC 1 cut(s) 779
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 8 cut(s) 38, 570, 598, 845, 1095, 1248, 1324, 1570
HphI GGTGA 3 cut(s) 455, 464, 743
Hpy166II GTNNAC 2 cut(s) 1031, 1282
Hpy188I TCNGA 6 cut(s) 463, 525, 642, 744, 1461, 1595
Hpy8I GTNNAC 2 cut(s) 1031, 1282
HpyAV CCTTC 6 cut(s) 577, 607, 1085, 1384, 1671, 1702
HpyCH4III ACNGT 6 cut(s) 34, 737, 1273, 1286, 1300, 1613
HpyCH4IV ACGT 1 cut(s) 1495
HpyF3I CTNAG 3 cut(s) 179, 741, 936
HpySE526I ACGT 1 cut(s) 1495
Hsp92II CATG 9 cut(s) 11, 50, 651, 892, 1163, 1370, 1582, 1624, 1703
HspAI GCGC 1 cut(s) 779
KpnI GGTACC 1 cut(s) 1657
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 4 cut(s) 106, 511, 903, 1456
LguI GCTCTTC 2 cut(s) 27, 642
LmnI GCTCC 1 cut(s) 530
Lsp1109I GCAGC 6 cut(s) 559, 724, 826, 919, 1587, 1683
LweI GCATC 3 cut(s) 1120, 1349, 1683
MabI ACCWGGT 1 cut(s) 1024
MaeI CTAG 5 cut(s) 212, 243, 360, 687, 1749
MaeII ACGT 1 cut(s) 1495
MaeIII GTNAC 3 cut(s) 316, 413, 731
MalI GATC 4 cut(s) 108, 513, 905, 1458
MboI GATC 4 cut(s) 106, 511, 903, 1456
MhlI GDGCHC 2 cut(s) 637, 1744
MlyI GAGTC 2 cut(s) 47, 1104
MmeI TCCRAC 1 cut(s) 1413
MseI TTAA 8 cut(s) 207, 818, 926, 1140, 1203, 1233, 1310, 1353
MspA1I CMGCKG 3 cut(s) 839, 1600, 1689
MspR9I CCNGG 1 cut(s) 1026
MvaI CCWGG 1 cut(s) 1026
MvnI CGCG 2 cut(s) 678, 781
NcoI CCATGG 1 cut(s) 1159
NdeII GATC 4 cut(s) 106, 511, 903, 1456
NlaIII CATG 9 cut(s) 11, 50, 651, 892, 1163, 1370, 1582, 1624, 1703
NlaIV GGNNCC 3 cut(s) 168, 1655, 1743
NmuCI GTSAC 1 cut(s) 731
NspV TTCGAA 1 cut(s) 1573
PagI TCATGA 3 cut(s) 46, 888, 1620
PciSI GCTCTTC 2 cut(s) 27, 642
PfeI GAWTC 6 cut(s) 570, 598, 845, 1248, 1324, 1570
PflMI CCANNNNNTGG 1 cut(s) 1176
PkrI GCNGC 8 cut(s) 549, 593, 714, 841, 934, 1602, 1688, 1698
PleI GAGTC 2 cut(s) 46, 1103
PpsI GAGTC 2 cut(s) 46, 1103
Psp6I CCWGG 1 cut(s) 1024
PspFI CCCAGC 1 cut(s) 1586
PspGI CCWGG 1 cut(s) 1024
PspN4I GGNNCC 3 cut(s) 168, 1655, 1743
PspPI GGNCC 1 cut(s) 1031
PstI CTGCAG 1 cut(s) 549
PstNI CAGNNNCTG 2 cut(s) 659, 842
PvuII CAGCTG 2 cut(s) 839, 1600
RsaI GTAC 4 cut(s) 71, 739, 1157, 1655
RsaNI GTAC 4 cut(s) 70, 738, 1156, 1654
SapI GCTCTTC 2 cut(s) 27, 642
SaqAI TTAA 8 cut(s) 207, 818, 926, 1140, 1203, 1233, 1310, 1353
SatI GCNGC 8 cut(s) 548, 592, 713, 840, 933, 1601, 1687, 1697
Sau3AI GATC 4 cut(s) 106, 511, 903, 1456
Sau96I GGNCC 1 cut(s) 1031
SchI GAGTC 2 cut(s) 47, 1104
ScrFI CCNGG 1 cut(s) 1026
SduI GDGCHC 2 cut(s) 637, 1744
SexAI ACCWGGT 1 cut(s) 1024
SfaNI GCATC 3 cut(s) 1120, 1349, 1683
SfcI CTRYAG 1 cut(s) 545
SfuI TTCGAA 1 cut(s) 1573
SinI GGWCC 1 cut(s) 1031
SmlI CTYRAG 2 cut(s) 1015, 1069
SmoI CTYRAG 2 cut(s) 1015, 1069
SsiI CCGC 7 cut(s) 140, 290, 428, 591, 676, 781, 1687
SspMI CTAG 5 cut(s) 212, 243, 360, 687, 1749
StyD4I CCNGG 1 cut(s) 1024
StyI CCWWGG 3 cut(s) 211, 1159, 1289
TaaI ACNGT 6 cut(s) 34, 737, 1273, 1286, 1300, 1613
TaiI ACGT 1 cut(s) 1498
TaqI TCGA 2 cut(s) 1081, 1573
TatI WGTACW 1 cut(s) 737
TauI GCSGC 2 cut(s) 594, 1689
TfiI GAWTC 6 cut(s) 570, 598, 845, 1248, 1324, 1570
Tru1I TTAA 8 cut(s) 207, 818, 926, 1140, 1203, 1233, 1310, 1353
Tru9I TTAA 8 cut(s) 207, 818, 926, 1140, 1203, 1233, 1310, 1353
TscAI CASTG 2 cut(s) 37, 1568
TseFI GTSAC 1 cut(s) 731
TseI GCWGC 6 cut(s) 547, 712, 839, 932, 1600, 1696
Tsp45I GTSAC 1 cut(s) 731
TspGWI ACGGA 1 cut(s) 965
TspRI CASTG 2 cut(s) 37, 1568
Van91I CCANNNNNTGG 1 cut(s) 1176
VpaK11BI GGWCC 1 cut(s) 1031
XagI CCTNNNNNAGG 1 cut(s) 548
XapI RAATTY 2 cut(s) 1306, 1318
XbaI TCTAGA 1 cut(s) 359
XmaJI CCTAGG 1 cut(s) 211
XspI CTAG 5 cut(s) 212, 243, 360, 687, 1749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.