RchiOBHm_Chr5g0065541

oxidosqualene cyclase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000624
Physical Location & Seq
Forward (+)
71498518 .. 71513229
14712 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34140

Sequence Viewer

Length: 2124 bp
ATGGATCCACGTTTCTCTGAATTTCCCAATTTCAATGATCAAACCCTTTTGCCCGATATGTTGAATGAGTACCCAAGTAGTGACCCTTTTGAAGATCTCAGCTTTTCGGACCCAAACCGCAGTAACTCTTCTCTGTCGTCGAGCTTTAGCGCTGAGGGAGATGATTGTGAGTTTTCTGATTGTGTGCTCAAGTACATAAACCAGGTGCTTATGGAGGAGGACATGGAGTCAAAGCCATGTATGTTCCATGACCCTTTGGCTCTTCAAGCCACTGAGGAGTCTCTTTATGAGGTTATTGGGGGAGGGAAGTACCCAATTTCCCAAGACCAAAACCCTCTTTGTGTTGATCAAAATGTTGAGAGCCCAGATGGTGGTTATTCTTCTGGAAGTTTCACTGATTCCAGCTCTAGTCCTGGTCCTTCCGTTAACTCTTGCCCAGTCACTGCCCAGTTTGTAGAGACTAGCAGCTCTATATTGGAAATCCCTATTCCTGAAAACTTTGTTTTCCAGTCCCCTTCCAGGGCTAGGTCTCAATTGTCGTCGAGTAGTAAGAATGAATTGTTAGTGCAGTTTAACCTAGGGGCAGAGGAGGGTAGGAAGTTTCTTCCTAGAGGTCAATTGATTAGTGTGGAGGGCAACAAGATGTATGCTGTGGTGAATAAAAAGGCTGGAGATGCGGTAGTAACAACGCAGAAGAATGGGAGTGAGCAATTTCATGTTGGTTCAAGAGGAAAAAAGAATCATCAAAGGGAAGATATAGTTTTGGAGGATGGGAGGAGTAACAAGCAACCAGCAGTTTATATGGACGATGAGGAGGGTGAGCTGTCTGATATTTTTGACAAGGTGTTGCTCTGTAAGCCTTTGGAGAGTAAAGACGATGAAGCCAGTCAGAATGGAGCAAACATTGGCCTGCAGCAGGATGTGCAATCTGTTGGAATTGGTAATGGGAAGGGTCGCGGCATGATTCACGGCAACAAGAAGGAAGTGATAGATTTGAGGGCTCTTCTGATTTTATGTGCACAAGCTGTTTCTGTTGATGATCGCGAGACTGCTAGTGAGCTACTAAAGCAGATTATGCAGCACTCTTCCCCATTTGGTGATTGTGCTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTGAAGCGAGGTTGGCTGGCACTGGAACTCAAATATATACTGCTTTATCTACTAAACGAATGTCAGCTGCTGATATGCTGAAGTATTACCAAGCTTATATTACAGCCTGCCCGTTGAATAAGATGTCCTTGATCTTTGCAAACGATATGATTTTAAAAGCTGCTGAGAAAGCAGAAACACTACATATAATAGATTTTGGTATCCTTTATGGTTTTCAATGGCCTGCTCTCATTCATTGCCTCTCAAGAAGACCTGGTGGACCTCCCAAATTACGTATTACAGGGGTAGAGCTTCCCTTAAGTGGTTTTCGACCAGAAGAAAGAGTCCAAGAGACGGGGCATCACTTGGCAAAGTATTGTAGGCGATTTAATGTTCCTTTTGAGTACTATGCCATAGCCAAAAAATGGGATACTGTCCAATTTGATGACCTCAAAGTTCAAAGAAATGAGGTGCTTGCTGTTAATTGTGTCTGCCGGTTTAAGAACCTCCTTGATGAGACAGTTGTGGTAAACAATCCAAGGGATACTGTTCTAAAATTAATTAGGAAGTTGAATCCTGATATCTTTGTCCACAGTGTTGTTAATGGATCCTATCATGCACCCTTCTTTGTTGCACGCTTCCGAGAGGCACTATTCCACTTCTCTGCATTTTTTGATATGTTTGATAACAATTTGCCTCGTGAAGATCAGATGAGATTGGGGTTTGAGAAAGAGTTTATTGGGCGTGAAGTTCTGAATATAGTAGCTTGTGAGGGCTCAGAAAGAATTGTGAGGCCAGAAACATATAAGCAGTGGCAGGTTCGGAACATGAGGGCTGGGTTCAGACAGCTGCCATTGGACCGTGAAGTCATGAACAAAATAAAGGATAAAGTAAAAATGGGGTACAGCCCTGATTTTGTGGTTGATGAAGATGGCCGCTGGATGCTGCTGGGATGGAAGGGCAGAATTATGTTTGGTTCTGCCTGTTGGGCACCGTCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 map00100 map00909 map01100 map01110
Pfam Domains
Protein Families
Prenyltrans SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

707

Amino Acids

79.44

Weight (kDa)

5.41

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 332 - 702 1.1e-117 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1982
Acc36I ACCTGC 1 cut(s) 1924
AccB1I GGYRCC 1 cut(s) 2107
AccB7I CCANNNNNTGG 2 cut(s) 371, 1542
AccII CGCG 2 cut(s) 957, 1044
AciI CCGC 4 cut(s) 118, 677, 957, 2053
AclWI GGATC 3 cut(s) 12, 1719, 1732
AcoI YGGCCR 1 cut(s) 2050
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 1 cut(s) 1238
AfaI GTAC 5 cut(s) 71, 194, 311, 1523, 2021
AfeI AGCGCT 1 cut(s) 151
AfiI CCNNNNNNNGG 8 cut(s) 371, 519, 520, 525, 916, 1439, 1471, 1542
AflII CTTAAG 1 cut(s) 1435
AhdI GACNNNNNGTC 1 cut(s) 226
AjnI CCWGG 4 cut(s) 201, 412, 518, 1390
Alw21I GWGCWC 3 cut(s) 189, 1021, 1108
Alw26I GTCTC 6 cut(s) 285, 452, 534, 1040, 1463, 1628
Alw44I GTGCAC 1 cut(s) 1017
AlwI GGATC 3 cut(s) 12, 1719, 1732
AlwNI CAGNNNCTG 2 cut(s) 443, 1208
Aor51HI AGCGCT 1 cut(s) 151
AoxI GGCC 4 cut(s) 907, 1358, 1910, 2050
ApaLI GTGCAC 1 cut(s) 1017
ApeKI GCWGC 7 cut(s) 465, 913, 1078, 1205, 1298, 1966, 2062
ApoI RAATTY 1 cut(s) 20
AseI ATTAAT 1 cut(s) 1676
AspA2I CCTAGG 1 cut(s) 577
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 4 cut(s) 109, 416, 1397, 1975
AsuHPI GGTGA 3 cut(s) 667, 830, 1109
AvaII GGWCC 4 cut(s) 109, 416, 1397, 1975
AvrII CCTAGG 1 cut(s) 577
BaeGI GKGCMC 2 cut(s) 1021, 2110
BamHI GGATCC 2 cut(s) 4, 1724
BanI GGYRCC 1 cut(s) 2107
BanII GRGCYC 3 cut(s) 365, 1003, 1895
BauI CACGAG 1 cut(s) 1815
BbsI GAAGAC 1 cut(s) 1393
Bbv12I GWGCWC 3 cut(s) 189, 1021, 1108
BbvCI CCTCAGC 1 cut(s) 153
BbvI GCAGC 7 cut(s) 477, 925, 1090, 1192, 1285, 1953, 2049
BccI CCATC 4 cut(s) 362, 764, 2042, 2064
BceAI ACGGC 1 cut(s) 985
BciT130I CCWGG 4 cut(s) 203, 414, 520, 1392
BciVI GTATCC 3 cut(s) 1349, 1540, 1654
BclI TGATCA 2 cut(s) 37, 346
BcoDI GTCTC 6 cut(s) 285, 452, 534, 1040, 1463, 1628
BfaI CTAG 7 cut(s) 408, 462, 525, 578, 609, 1053, 2115
BfmI CTRYAG 1 cut(s) 911
BfoI RGCGCY 1 cut(s) 153
BfrI CTTAAG 1 cut(s) 1435
BfuAI ACCTGC 1 cut(s) 1924
BfuI GTATCC 3 cut(s) 1349, 1540, 1654
BglI GCCNNNNNGGC 1 cut(s) 2105
BglII AGATCT 1 cut(s) 94
BisI GCNGC 9 cut(s) 466, 914, 958, 1079, 1206, 1299, 1967, 2053, 2063
BlnI CCTAGG 1 cut(s) 577
BlsI GCNGC 9 cut(s) 467, 915, 959, 1080, 1207, 1300, 1968, 2054, 2064
BmcAI AGTACT 1 cut(s) 1523
Bme1390I CCNGG 4 cut(s) 203, 414, 520, 1392
Bme18I GGWCC 4 cut(s) 109, 416, 1397, 1975
BmeRI GACNNNNNGTC 1 cut(s) 226
BmgT120I GGNCC 4 cut(s) 109, 416, 1397, 1975
BmiI GGNNCC 4 cut(s) 6, 111, 1726, 2109
BmrFI CCNGG 4 cut(s) 203, 414, 520, 1392
BmrI ACTGGG 2 cut(s) 431, 442
BmsI GCATC 3 cut(s) 664, 1486, 2049
BmuI ACTGGG 2 cut(s) 431, 442
BpiI GAAGAC 1 cut(s) 1393
BplI GAGNNNNNCTC 2 cut(s) 1102, 1134
BpmI CTGGAG 1 cut(s) 690
Bpu10I CCTNAGC 1 cut(s) 153
BpuEI CTTGAG 2 cut(s) 173, 1366
BsaAI YACGTR 1 cut(s) 1412
BsaI GGTCTC 1 cut(s) 534
BsaJI CCNNGG 3 cut(s) 519, 577, 1655
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 8 cut(s) 371, 519, 520, 525, 916, 1439, 1471, 1542
Bse118I RCCGGY 1 cut(s) 1611
Bse1I ACTGG 5 cut(s) 437, 448, 508, 885, 1165
Bse3DI GCAATG 2 cut(s) 1120, 1372
BseBI CCWGG 4 cut(s) 203, 414, 520, 1392
BseDI CCNNGG 3 cut(s) 519, 577, 1655
BseGI GGATG 4 cut(s) 775, 925, 2064, 2075
BseLI CCNNNNNNNGG 8 cut(s) 371, 519, 520, 525, 916, 1439, 1471, 1542
BseMI GCAATG 2 cut(s) 1120, 1372
BseMII CTCAG 6 cut(s) 112, 144, 264, 1121, 1293, 1908
BseNI ACTGG 5 cut(s) 437, 448, 508, 885, 1165
BseRI GAGGAG 5 cut(s) 230, 290, 602, 790, 827
BseSI GKGCMC 2 cut(s) 1021, 2110
BseXI GCAGC 7 cut(s) 477, 925, 1090, 1192, 1285, 1953, 2049
BseYI CCCAGC 2 cut(s) 1952, 2065
BsgI GTGCAG 1 cut(s) 587
Bsh1236I CGCG 2 cut(s) 957, 1044
BshFI GGCC 4 cut(s) 909, 1360, 1912, 2052
BshNI GGYRCC 1 cut(s) 2107
BsiHKAI GWGCWC 3 cut(s) 189, 1021, 1108
BsiSI CCGG 1 cut(s) 1612
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 8 cut(s) 371, 519, 520, 525, 916, 1439, 1471, 1542
BsmAI GTCTC 6 cut(s) 285, 452, 534, 1040, 1463, 1628
BsmBI CGTCTC 1 cut(s) 1463
BsmFI GGGAC 1 cut(s) 496
BsnI GGCC 4 cut(s) 909, 1360, 1912, 2052
Bso31I GGTCTC 1 cut(s) 534
Bsp1286I GDGCHC 7 cut(s) 189, 365, 1003, 1021, 1108, 1895, 2110
Bsp143I GATC 8 cut(s) 4, 37, 94, 346, 1039, 1269, 1724, 1822
Bsp68I TCGCGA 1 cut(s) 1044
BspACI CCGC 4 cut(s) 118, 677, 957, 2053
BspANI GGCC 4 cut(s) 909, 1360, 1912, 2052
BspCNI CTCAG 6 cut(s) 111, 145, 265, 1120, 1294, 1907
BspFNI CGCG 2 cut(s) 957, 1044
BspHI TCATGA 1 cut(s) 1986
BspLI GGNNCC 4 cut(s) 6, 111, 1726, 2109
BspMAI CTGCAG 1 cut(s) 915
BspMI ACCTGC 1 cut(s) 1924
BspPI GGATC 3 cut(s) 12, 1719, 1732
BspQI GCTCTTC 2 cut(s) 267, 1008
BspT107I GGYRCC 1 cut(s) 2107
BspTI CTTAAG 1 cut(s) 1435
BspTNI GGTCTC 1 cut(s) 534
BsrDI GCAATG 2 cut(s) 1120, 1372
BsrFI RCCGGY 1 cut(s) 1611
BsrI ACTGG 5 cut(s) 437, 448, 508, 885, 1165
BssAI RCCGGY 1 cut(s) 1611
BssECI CCNNGG 3 cut(s) 519, 577, 1655
BssMI GATC 8 cut(s) 4, 37, 94, 346, 1039, 1269, 1724, 1822
BssSI CACGAG 1 cut(s) 1815
BssT1I CCWWGG 2 cut(s) 577, 1655
Bst2BI CACGAG 1 cut(s) 1815
Bst2UI CCWGG 4 cut(s) 203, 414, 520, 1392
Bst4CI ACNGT 6 cut(s) 1552, 1639, 1666, 1712, 1979, 2112
Bst6I CTCTTC 4 cut(s) 133, 267, 1008, 1090
BstAFI CTTAAG 1 cut(s) 1435
BstAPI GCANNNNNTGC 2 cut(s) 922, 1075
BstBAI YACGTR 1 cut(s) 1412
BstC8I GCNNGC 6 cut(s) 911, 1156, 1246, 1362, 1593, 1753
BstDEI CTNAG 6 cut(s) 98, 153, 273, 1107, 1302, 1894
BstENI CCTNNNNNAGG 1 cut(s) 914
BstF5I GGATG 4 cut(s) 775, 925, 2064, 2075
BstFNI CGCG 2 cut(s) 957, 1044
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 8 cut(s) 7, 40, 97, 349, 1042, 1272, 1727, 1825
BstMAI GTCTC 6 cut(s) 285, 452, 534, 1040, 1463, 1628
BstMBI GATC 8 cut(s) 4, 37, 94, 346, 1039, 1269, 1724, 1822
BstNI CCWGG 4 cut(s) 203, 414, 520, 1392
BstSCI CCNGG 4 cut(s) 201, 412, 518, 1390
BstSFI CTRYAG 1 cut(s) 911
BstSLI GKGCMC 2 cut(s) 1021, 2110
BstSNI TACGTA 1 cut(s) 1412
BstUI CGCG 2 cut(s) 957, 1044
BstV1I GCAGC 7 cut(s) 477, 925, 1090, 1192, 1285, 1953, 2049
BstV2I GAAGAC 1 cut(s) 1393
BstX2I RGATCY 3 cut(s) 4, 94, 1724
BstYI RGATCY 3 cut(s) 4, 94, 1724
BsuI GTATCC 3 cut(s) 1349, 1540, 1654
BsuRI GGCC 4 cut(s) 909, 1360, 1912, 2052
BtsCI GGATG 4 cut(s) 775, 925, 2064, 2075
BtsI GCAGTG 2 cut(s) 441, 1934
BtsIMutI CAGTG 6 cut(s) 270, 393, 441, 1158, 1717, 1934
BtuMI TCGCGA 1 cut(s) 1044
BveI ACCTGC 1 cut(s) 1924
Cac8I GCNNGC 6 cut(s) 911, 1156, 1246, 1362, 1593, 1753
CaiI CAGNNNCTG 2 cut(s) 443, 1208
CciI TCATGA 1 cut(s) 1986
CfoI GCGC 1 cut(s) 152
Cfr10I RCCGGY 1 cut(s) 1611
Cfr13I GGNCC 4 cut(s) 109, 416, 1397, 1975
CsiI ACCWGGT 2 cut(s) 201, 1390
Csp6I GTAC 5 cut(s) 70, 193, 310, 1522, 2020
CviAII CATG 8 cut(s) 223, 237, 248, 716, 961, 1733, 1945, 1987
CviQI GTAC 5 cut(s) 70, 193, 310, 1522, 2020
DdeI CTNAG 6 cut(s) 98, 153, 273, 1107, 1302, 1894
DpnI GATC 8 cut(s) 6, 39, 96, 348, 1041, 1271, 1726, 1824
DpnII GATC 8 cut(s) 4, 37, 94, 346, 1039, 1269, 1724, 1822
DraI TTTAAA 1 cut(s) 1293
DrdI GACNNNNNNGTC 1 cut(s) 1982
DriI GACNNNNNGTC 1 cut(s) 226
DseDI GACNNNNNNGTC 1 cut(s) 1982
EaeI YGGCCR 1 cut(s) 2050
Eam1104I CTCTTC 4 cut(s) 133, 267, 1008, 1090
Eam1105I GACNNNNNGTC 1 cut(s) 226
EarI CTCTTC 4 cut(s) 133, 267, 1008, 1090
Eco105I TACGTA 1 cut(s) 1412
Eco130I CCWWGG 2 cut(s) 577, 1655
Eco24I GRGCYC 3 cut(s) 365, 1003, 1895
Eco31I GGTCTC 1 cut(s) 534
Eco32I GATATC 1 cut(s) 1699
Eco47I GGWCC 4 cut(s) 109, 416, 1397, 1975
Eco47III AGCGCT 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 1238
EcoNI CCTNNNNNAGG 1 cut(s) 914
EcoRII CCWGG 4 cut(s) 201, 412, 518, 1390
EcoRV GATATC 1 cut(s) 1699
EcoT14I CCWWGG 2 cut(s) 577, 1655
EcoT38I GRGCYC 3 cut(s) 365, 1003, 1895
ErhI CCWWGG 2 cut(s) 577, 1655
Esp3I CGTCTC 1 cut(s) 1463
FaeI CATG 8 cut(s) 226, 240, 251, 719, 964, 1736, 1948, 1990
FalI AAGNNNNNCTT 2 cut(s) 1250, 1282
FaqI GGGAC 1 cut(s) 496
FatI CATG 8 cut(s) 222, 236, 247, 715, 960, 1732, 1944, 1986
FbaI TGATCA 2 cut(s) 37, 346
Fnu4HI GCNGC 9 cut(s) 466, 914, 958, 1079, 1206, 1299, 1967, 2053, 2063
FokI GGATG 4 cut(s) 782, 932, 2071, 2082
FriOI GRGCYC 3 cut(s) 365, 1003, 1895
Fsp4HI GCNGC 9 cut(s) 466, 914, 958, 1079, 1206, 1299, 1967, 2053, 2063
FspBI CTAG 7 cut(s) 408, 462, 525, 578, 609, 1053, 2115
GlaI GCGC 1 cut(s) 151
GluI GCNGC 9 cut(s) 466, 914, 958, 1079, 1206, 1299, 1967, 2053, 2063
GsaI CCCAGC 2 cut(s) 1956, 2069
GsuI CTGGAG 1 cut(s) 690
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 4 cut(s) 909, 1360, 1912, 2052
HapII CCGG 1 cut(s) 1612
HhaI GCGC 1 cut(s) 152
Hin1II CATG 8 cut(s) 226, 240, 251, 719, 964, 1736, 1948, 1990
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 1230
HinfI GANTC 7 cut(s) 227, 278, 398, 739, 964, 1461, 1690
HpaI GTTAAC 1 cut(s) 427
HpaII CCGG 1 cut(s) 1612
HphI GGTGA 3 cut(s) 667, 830, 1109
Hpy166II GTNNAC 5 cut(s) 427, 1019, 1397, 1648, 1708
Hpy188III TCNNGA 9 cut(s) 384, 491, 726, 1043, 1139, 1383, 1694, 1817, 1987
Hpy8I GTNNAC 5 cut(s) 427, 1019, 1397, 1648, 1708
Hpy99I CGWCG 2 cut(s) 142, 544
HpyAV CCTTC 6 cut(s) 429, 525, 943, 973, 1750, 2068
HpyCH4III ACNGT 6 cut(s) 1552, 1639, 1666, 1712, 1979, 2112
HpyCH4IV ACGT 2 cut(s) 10, 1411
HpyF3I CTNAG 6 cut(s) 98, 153, 273, 1107, 1302, 1894
HpySE526I ACGT 2 cut(s) 10, 1411
Hsp92II CATG 8 cut(s) 226, 240, 251, 719, 964, 1736, 1948, 1990
HspAI GCGC 1 cut(s) 150
Ksp22I TGATCA 2 cut(s) 37, 346
KspAI GTTAAC 1 cut(s) 427
Kzo9I GATC 8 cut(s) 4, 37, 94, 346, 1039, 1269, 1724, 1822
LguI GCTCTTC 2 cut(s) 267, 1008
LmnI GCTCC 1 cut(s) 896
Lsp1109I GCAGC 7 cut(s) 477, 925, 1090, 1192, 1285, 1953, 2049
LweI GCATC 3 cut(s) 664, 1486, 2049
MabI ACCWGGT 2 cut(s) 201, 1390
MaeI CTAG 7 cut(s) 408, 462, 525, 578, 609, 1053, 2115
MaeII ACGT 2 cut(s) 10, 1411
MaeIII GTNAC 5 cut(s) 80, 122, 439, 682, 779
MalI GATC 8 cut(s) 6, 39, 96, 348, 1041, 1271, 1726, 1824
MboI GATC 8 cut(s) 4, 37, 94, 346, 1039, 1269, 1724, 1822
MfeI CAATTG 2 cut(s) 533, 617
MflI RGATCY 3 cut(s) 4, 94, 1724
MhlI GDGCHC 7 cut(s) 189, 365, 1003, 1021, 1108, 1895, 2110
MlyI GAGTC 3 cut(s) 236, 287, 1470
MmeI TCCRAC 1 cut(s) 913
MseI TTAA 9 cut(s) 426, 573, 1292, 1436, 1506, 1599, 1617, 1676, 1719
MspA1I CMGCKG 3 cut(s) 1205, 1966, 2055
MspCI CTTAAG 1 cut(s) 1435
MspI CCGG 1 cut(s) 1612
MspR9I CCNGG 4 cut(s) 203, 414, 520, 1392
MunI CAATTG 2 cut(s) 533, 617
MvaI CCWGG 4 cut(s) 203, 414, 520, 1392
MvnI CGCG 2 cut(s) 957, 1044
NdeII GATC 8 cut(s) 4, 37, 94, 346, 1039, 1269, 1724, 1822
NlaIII CATG 8 cut(s) 226, 240, 251, 719, 964, 1736, 1948, 1990
NlaIV GGNNCC 4 cut(s) 6, 111, 1726, 2109
NmuCI GTSAC 2 cut(s) 80, 439
NruI TCGCGA 1 cut(s) 1044
PagI TCATGA 1 cut(s) 1986
PciSI GCTCTTC 2 cut(s) 267, 1008
PfeI GAWTC 4 cut(s) 398, 739, 964, 1690
PflMI CCANNNNNTGG 2 cut(s) 371, 1542
PkrI GCNGC 9 cut(s) 467, 915, 959, 1080, 1207, 1300, 1968, 2054, 2064
PleI GAGTC 3 cut(s) 235, 286, 1469
PpsI GAGTC 3 cut(s) 235, 286, 1469
Ppu21I YACGTR 1 cut(s) 1412
PshBI ATTAAT 1 cut(s) 1676
Psp6I CCWGG 4 cut(s) 201, 412, 518, 1390
PspFI CCCAGC 2 cut(s) 1952, 2065
PspGI CCWGG 4 cut(s) 201, 412, 518, 1390
PspN4I GGNNCC 4 cut(s) 6, 111, 1726, 2109
PspPI GGNCC 4 cut(s) 109, 416, 1397, 1975
PstI CTGCAG 1 cut(s) 915
PstNI CAGNNNCTG 2 cut(s) 443, 1208
PsuI RGATCY 3 cut(s) 4, 94, 1724
PvuII CAGCTG 2 cut(s) 1205, 1966
RruI TCGCGA 1 cut(s) 1044
RsaI GTAC 5 cut(s) 71, 194, 311, 1523, 2021
RsaNI GTAC 5 cut(s) 70, 193, 310, 1522, 2020
SapI GCTCTTC 2 cut(s) 267, 1008
SaqAI TTAA 9 cut(s) 426, 573, 1292, 1436, 1506, 1599, 1617, 1676, 1719
SatI GCNGC 9 cut(s) 466, 914, 958, 1079, 1206, 1299, 1967, 2053, 2063
Sau3AI GATC 8 cut(s) 4, 37, 94, 346, 1039, 1269, 1724, 1822
Sau96I GGNCC 4 cut(s) 109, 416, 1397, 1975
ScaI AGTACT 1 cut(s) 1523
SchI GAGTC 3 cut(s) 236, 287, 1470
ScrFI CCNGG 4 cut(s) 203, 414, 520, 1392
SduI GDGCHC 7 cut(s) 189, 365, 1003, 1021, 1108, 1895, 2110
SexAI ACCWGGT 2 cut(s) 201, 1390
SfaNI GCATC 3 cut(s) 664, 1486, 2049
SfcI CTRYAG 1 cut(s) 911
SinI GGWCC 4 cut(s) 109, 416, 1397, 1975
SmlI CTYRAG 3 cut(s) 188, 1381, 1435
SmoI CTYRAG 3 cut(s) 188, 1381, 1435
SnaBI TACGTA 1 cut(s) 1412
SsiI CCGC 4 cut(s) 118, 677, 957, 2053
SspMI CTAG 7 cut(s) 408, 462, 525, 578, 609, 1053, 2115
StyD4I CCNGG 4 cut(s) 201, 412, 518, 1390
StyI CCWWGG 2 cut(s) 577, 1655
TaaI ACNGT 6 cut(s) 1552, 1639, 1666, 1712, 1979, 2112
TaiI ACGT 2 cut(s) 13, 1414
TaqI TCGA 3 cut(s) 140, 542, 1447
TatI WGTACW 2 cut(s) 192, 1521
TauI GCSGC 2 cut(s) 960, 2055
TfiI GAWTC 4 cut(s) 398, 739, 964, 1690
Tru1I TTAA 9 cut(s) 426, 573, 1292, 1436, 1506, 1599, 1617, 1676, 1719
Tru9I TTAA 9 cut(s) 426, 573, 1292, 1436, 1506, 1599, 1617, 1676, 1719
TscAI CASTG 6 cut(s) 277, 400, 448, 1165, 1717, 1934
TseFI GTSAC 2 cut(s) 80, 439
TseI GCWGC 7 cut(s) 465, 913, 1078, 1205, 1298, 1966, 2062
Tsp45I GTSAC 2 cut(s) 80, 439
TspDTI ATGAA 6 cut(s) 570, 704, 894, 1361, 2003, 2058
TspGWI ACGGA 1 cut(s) 412
TspRI CASTG 6 cut(s) 277, 400, 448, 1165, 1717, 1934
Van91I CCANNNNNTGG 2 cut(s) 371, 1542
Vha464I CTTAAG 1 cut(s) 1435
VneI GTGCAC 1 cut(s) 1017
VpaK11BI GGWCC 4 cut(s) 109, 416, 1397, 1975
VspI ATTAAT 1 cut(s) 1676
XagI CCTNNNNNAGG 1 cut(s) 914
XapI RAATTY 1 cut(s) 20
XmaJI CCTAGG 1 cut(s) 577
XspI CTAG 7 cut(s) 408, 462, 525, 578, 609, 1053, 2115
ZrmI AGTACT 1 cut(s) 1523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.