RLG00000035794

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000624
Physical Location & Seq
Forward (+)
71717649 .. 71719640
1992 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035794

Sequence Viewer

Length: 1992 bp
ATGGATCCACGTTTCTCTGAATTTCCCAATTTCAATGATCACACCCTTTTGCCTGATATGTTGACTGAGTACCCAAGTAGTGATCCTTCTCAAGATCTTAGCTTTGTGGACTCAAATCCCATTAACTCTTCATCAAGCTTTAGCCCTGAGGGAGATGATGGTGAGTTTTCTGATGGTGTGCTCAAGTACATAAACCAGGTGCTTATTAAGGAGGACATGGAATCAAAACCCTGTATGTTCCATGACCCTTTGGCTCTTCAAGCCACTGAGGAGTCTCTTCATGAGGTTATTGGAGGAGGGAAGTACCCAACTTCCCAAGACCTAAACCCTCTTTGTGCTGATCAAGATGTGTACGGAAATGGAAATGGGATGGTGGGTTCTTATAGGAGGGAGCTAATAGTTCCTGATGTGCTTAGTAAGAATGAATTGTTATTGCAATTTAACCTAGGGGCAGAGGAGGGTGGTAAGTTGCTTCCTAGAGGTCAGTTGATTAGTGCGGAGGGCAACAAGCCGTATGCTGTGGCGGATAGAAAGGCTGGAGATGTGGTAGTTACGACCCAGAAGAATGGGAGTGAGCATTTTCGTGTTGGTTTTACAGGAAAGAAAAATCATCAAAGGGAAGATATAGATTTGGAGGATGGCAGGAGTAACAAGCAATCGGCTGTTTATATGGATGGTGAAGAGGGTGAGCTGTCTGATATTTTTGACAAGGTGTTGCTCTCCAAGCCTATCAAGAGTAAAGACGATCAAGTCAGTCAGGATGGAGCATACAATAGCCTGCAGCAGGATGGGCTATCTGTTGGAATTGGTAATGGGAAGGTTTGCGGCAAGAATCAAGGCAACAAGAAGGAAGTGATAGATTTGAGTGCTCTTCTGATTTCATGTGCAGAAGCTGTATCTGTTGATGACCGTGAGACTGCTAGTGAGCTACTAAGGCAGATTATGCAGCACTCTTCCCCATTTGGTGATTGTACTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTCAAGTGCGGTTGGCTGGCACAGGAACTCAAATATATACTGCTTTAACTACTAAACGAATGTCAGCTGCCGATTCGCTGAAGTTTTACCGAGCTTATATTGCAGTCTGCCCGTTCATGAAGTTGTTGATGATCTTTGCAAACGATATGATTTTAAAAGCTACTGAGAAAGCAGAAACGCTACATATAATAGATTTTGGTATCCGTTATGGTTTTCAATGGCCTGCTCTCATTCATTGCCTCTCAAGAAGACCTGGTGGACCTCCGAAATTACGCATTACAGGGTTAGAGTTTCCCTCAAGTGGTTTTCGACCAGAAGAAGGAGTCCAAGAGACAGGGCATCGCTTGGCAAAGTATTGTAGGCGATTTAATGTTCATTTTGAGTACCATGGCATAGCCAAAAAATGGGAAACTCTCCAAGTTGATGACCTTAAAGTTCAAAGAAATGAGGTGCTTGCTGTAAATTGTCTCAACCGATTCAAGTACCTCCTTGATGAGACGGTTGTGGTAAACAGTCCAAGGGATACTGTTCTAAATTTAATTAGGAATTTGAATCCTGATGTCTTTGTCCAAAGTGTTGTTAATGGATCCCGTCATGCACCCTTCTTAGTTCCACGCTTCCGAGAGGCACTATTCCACTTCTCTGCATTGTTTGATATGTTGGATACCAATTTGCCTCGTGAAGATCAGATGAGATTGGGGTTTGAGAAAGAGTTTATTGGACGTGAAGTTGTCAATATAGTAGCTTGTGAGGGCTTTCAAAGAATTTTCAGGCCAGAAACATACAAGCAATGGCAGATTCGAAACATGAGGGCTGGGTTCAGACAGCTGCCATTGGACCGTGAAGTCATGAACAAAATAAAGGATAAGGTAAAAATGGGGTACCACCCTTGTTTTGTGGTTGATGAAGATGGCGGCTGGATGCTGCATGAATGGAAGGGCAGAATTATGTATGCTTCTGCCTGTTGGGCACCATCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

664

Amino Acids

74.73

Weight (kDa)

5.82

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 289 - 658 7.2e-116 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 749, 1850
Acc65I GGTACC 1 cut(s) 1887
AccB1I GGYRCC 2 cut(s) 1887, 1975
AccB7I CCANNNNNTGG 1 cut(s) 1410
AciI CCGC 5 cut(s) 497, 524, 825, 1015, 1920
AclWI GGATC 4 cut(s) 12, 77, 1587, 1600
AcsI RAATTY 4 cut(s) 20, 1540, 1552, 1770
AcuI CTGAAG 1 cut(s) 1106
AfaI GTAC 8 cut(s) 71, 188, 305, 353, 973, 1391, 1490, 1889
AfiI CCNNNNNNNGG 4 cut(s) 784, 1307, 1325, 1410
AgsI TTSAA 8 cut(s) 34, 260, 1010, 1223, 1445, 1486, 1558, 1766
AjiI CACGTC 1 cut(s) 1730
AjnI CCWGG 2 cut(s) 195, 1258
AloI GAACNNNNNNTCC 2 cut(s) 361, 393
Alw21I GWGCWC 2 cut(s) 183, 871
Alw26I GTCTC 5 cut(s) 279, 908, 1331, 1478, 1496
AlwI GGATC 4 cut(s) 12, 77, 1587, 1600
AlwNI CAGNNNCTG 1 cut(s) 893
AoxI GGCC 2 cut(s) 1226, 1778
ApeKI GCWGC 5 cut(s) 781, 946, 1073, 1834, 1930
ApoI RAATTY 4 cut(s) 20, 1540, 1552, 1770
Asp718I GGTACC 1 cut(s) 1887
AspA2I CCTAGG 1 cut(s) 445
AspS9I GGNCC 2 cut(s) 1265, 1843
AsuHPI GGTGA 4 cut(s) 173, 689, 698, 977
AsuII TTCGAA 1 cut(s) 1807
AvaII GGWCC 2 cut(s) 1265, 1843
AvrII CCTAGG 1 cut(s) 445
AxyI CCTNAGG 1 cut(s) 147
BaeGI GKGCMC 1 cut(s) 1978
BamHI GGATCC 2 cut(s) 4, 1592
BanI GGYRCC 2 cut(s) 1887, 1975
BarI GAAGNNNNNNTAC 2 cut(s) 70, 102
BauI CACGAG 1 cut(s) 1683
BbsI GAAGAC 1 cut(s) 1261
Bbv12I GWGCWC 2 cut(s) 183, 871
BbvI GCAGC 5 cut(s) 793, 958, 1060, 1821, 1917
BccI CCATC 9 cut(s) 152, 167, 364, 632, 668, 755, 782, 1910, 1987
BceAI ACGGC 1 cut(s) 496
BciT130I CCWGG 2 cut(s) 197, 1260
BciVI GTATCC 3 cut(s) 1217, 1522, 1663
BclI TGATCA 2 cut(s) 37, 340
BcoDI GTCTC 5 cut(s) 279, 908, 1331, 1478, 1496
BfaI CTAG 4 cut(s) 446, 477, 921, 1983
BfmI CTRYAG 1 cut(s) 779
BfuI GTATCC 3 cut(s) 1217, 1522, 1663
BglI GCCNNNNNGGC 1 cut(s) 1973
BglII AGATCT 1 cut(s) 94
BisI GCNGC 7 cut(s) 782, 826, 947, 1074, 1835, 1921, 1931
BlnI CCTAGG 1 cut(s) 445
BlsI GCNGC 7 cut(s) 783, 827, 948, 1075, 1836, 1922, 1932
Bme1390I CCNGG 2 cut(s) 197, 1260
Bme18I GGWCC 2 cut(s) 1265, 1843
BmgBI CACGTC 1 cut(s) 1730
BmgT120I GGNCC 2 cut(s) 1265, 1843
BmiI GGNNCC 4 cut(s) 6, 1594, 1889, 1977
BmrFI CCNGG 2 cut(s) 197, 1260
BmsI GCATC 2 cut(s) 1354, 1917
BpiI GAAGAC 1 cut(s) 1261
BplI GAGNNNNNCTC 4 cut(s) 970, 1002, 1286, 1318
BpmI CTGGAG 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 1807
BpuEI CTTGAG 4 cut(s) 75, 167, 1234, 1288
BsaJI CCNNGG 3 cut(s) 445, 1393, 1523
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 4 cut(s) 784, 1307, 1325, 1410
Bse21I CCTNAGG 1 cut(s) 147
Bse3DI GCAATG 3 cut(s) 988, 1240, 1802
BseBI CCWGG 2 cut(s) 197, 1260
BseDI CCNNGG 3 cut(s) 445, 1393, 1523
BseGI GGATG 6 cut(s) 375, 643, 679, 766, 793, 1932
BseLI CCNNNNNNNGG 4 cut(s) 784, 1307, 1325, 1410
BseMI GCAATG 3 cut(s) 988, 1240, 1802
BseMII CTCAG 5 cut(s) 57, 138, 258, 989, 1161
BseRI GAGGAG 3 cut(s) 284, 309, 470
BseSI GKGCMC 1 cut(s) 1978
BseXI GCAGC 5 cut(s) 793, 958, 1060, 1821, 1917
BseYI CCCAGC 1 cut(s) 1820
BsgI GTGCAG 1 cut(s) 906
BshFI GGCC 2 cut(s) 1228, 1780
BshNI GGYRCC 2 cut(s) 1887, 1975
BsiHKAI GWGCWC 2 cut(s) 183, 871
BslI CCNNNNNNNGG 4 cut(s) 784, 1307, 1325, 1410
BsmAI GTCTC 5 cut(s) 279, 908, 1331, 1478, 1496
BsmBI CGTCTC 1 cut(s) 1496
BsnI GGCC 2 cut(s) 1228, 1780
Bsp119I TTCGAA 1 cut(s) 1807
Bsp1286I GDGCHC 3 cut(s) 183, 871, 1978
Bsp143I GATC 9 cut(s) 4, 37, 82, 94, 340, 745, 1137, 1592, 1690
Bsp19I CCATGG 1 cut(s) 1393
BspACI CCGC 5 cut(s) 497, 524, 825, 1015, 1920
BspANI GGCC 2 cut(s) 1228, 1780
BspCNI CTCAG 5 cut(s) 58, 139, 259, 988, 1162
BspHI TCATGA 3 cut(s) 280, 1122, 1854
BspLI GGNNCC 4 cut(s) 6, 1594, 1889, 1977
BspMAI CTGCAG 1 cut(s) 783
BspPI GGATC 4 cut(s) 12, 77, 1587, 1600
BspQI GCTCTTC 3 cut(s) 261, 876, 1011
BspT104I TTCGAA 1 cut(s) 1807
BspT107I GGYRCC 2 cut(s) 1887, 1975
BsrDI GCAATG 3 cut(s) 988, 1240, 1802
BssECI CCNNGG 3 cut(s) 445, 1393, 1523
BssMI GATC 9 cut(s) 4, 37, 82, 94, 340, 745, 1137, 1592, 1690
BssSI CACGAG 1 cut(s) 1683
BssT1I CCWWGG 3 cut(s) 445, 1393, 1523
Bst2BI CACGAG 1 cut(s) 1683
Bst2UI CCWGG 2 cut(s) 197, 1260
Bst4CI ACNGT 5 cut(s) 911, 1507, 1520, 1534, 1847
Bst6I CTCTTC 7 cut(s) 133, 261, 282, 675, 876, 958, 1011
BstAPI GCANNNNNTGC 1 cut(s) 943
BstBI TTCGAA 1 cut(s) 1807
BstC8I GCNNGC 4 cut(s) 779, 1024, 1230, 1461
BstDEI CTNAG 9 cut(s) 66, 98, 147, 267, 413, 932, 975, 1170, 1612
BstDSI CCRYGG 1 cut(s) 1393
BstENI CCTNNNNNAGG 1 cut(s) 782
BstF5I GGATG 6 cut(s) 375, 643, 679, 766, 793, 1932
BstKTI GATC 9 cut(s) 7, 40, 85, 97, 343, 748, 1140, 1595, 1693
BstMAI GTCTC 5 cut(s) 279, 908, 1331, 1478, 1496
BstMBI GATC 9 cut(s) 4, 37, 82, 94, 340, 745, 1137, 1592, 1690
BstMWI GCNNNNNNNGC 7 cut(s) 260, 724, 790, 934, 943, 1106, 1973
BstNI CCWGG 2 cut(s) 197, 1260
BstSCI CCNGG 2 cut(s) 195, 1258
BstSFI CTRYAG 1 cut(s) 779
BstSLI GKGCMC 1 cut(s) 1978
BstV1I GCAGC 5 cut(s) 793, 958, 1060, 1821, 1917
BstV2I GAAGAC 1 cut(s) 1261
BstX2I RGATCY 3 cut(s) 4, 94, 1592
BstXI CCANNNNNNTGG 1 cut(s) 566
BstYI RGATCY 3 cut(s) 4, 94, 1592
Bsu36I CCTNAGG 1 cut(s) 147
BsuI GTATCC 3 cut(s) 1217, 1522, 1663
BsuRI GGCC 2 cut(s) 1228, 1780
BtgI CCRYGG 1 cut(s) 1393
BtgZI GCGATG 1 cut(s) 1331
BtrI CACGTC 1 cut(s) 1730
BtsCI GGATG 6 cut(s) 375, 643, 679, 766, 793, 1932
BtsIMutI CAGTG 1 cut(s) 264
Cac8I GCNNGC 4 cut(s) 779, 1024, 1230, 1461
CaiI CAGNNNCTG 1 cut(s) 893
CciI TCATGA 3 cut(s) 280, 1122, 1854
Cfr13I GGNCC 2 cut(s) 1265, 1843
CsiI ACCWGGT 2 cut(s) 195, 1258
Csp6I GTAC 8 cut(s) 70, 187, 304, 352, 972, 1390, 1489, 1888
CviQI GTAC 8 cut(s) 70, 187, 304, 352, 972, 1390, 1489, 1888
DdeI CTNAG 9 cut(s) 66, 98, 147, 267, 413, 932, 975, 1170, 1612
DpnI GATC 9 cut(s) 6, 39, 84, 96, 342, 747, 1139, 1594, 1692
DpnII GATC 9 cut(s) 4, 37, 82, 94, 340, 745, 1137, 1592, 1690
DraI TTTAAA 1 cut(s) 1161
DrdI GACNNNNNNGTC 2 cut(s) 749, 1850
DseDI GACNNNNNNGTC 2 cut(s) 749, 1850
Eam1104I CTCTTC 7 cut(s) 133, 261, 282, 675, 876, 958, 1011
EarI CTCTTC 7 cut(s) 133, 261, 282, 675, 876, 958, 1011
EciI GGCGGA 1 cut(s) 539
Eco130I CCWWGG 3 cut(s) 445, 1393, 1523
Eco47I GGWCC 2 cut(s) 1265, 1843
Eco57I CTGAAG 1 cut(s) 1106
Eco81I CCTNAGG 1 cut(s) 147
EcoNI CCTNNNNNAGG 1 cut(s) 782
EcoRII CCWGG 2 cut(s) 195, 1258
EcoT14I CCWWGG 3 cut(s) 445, 1393, 1523
ErhI CCWWGG 3 cut(s) 445, 1393, 1523
Esp3I CGTCTC 1 cut(s) 1496
FbaI TGATCA 2 cut(s) 37, 340
Fnu4HI GCNGC 7 cut(s) 782, 826, 947, 1074, 1835, 1921, 1931
FokI GGATG 6 cut(s) 382, 650, 686, 773, 800, 1939
Fsp4HI GCNGC 7 cut(s) 782, 826, 947, 1074, 1835, 1921, 1931
FspBI CTAG 4 cut(s) 446, 477, 921, 1983
GluI GCNGC 7 cut(s) 782, 826, 947, 1074, 1835, 1921, 1931
GsaI CCCAGC 1 cut(s) 1824
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 2 cut(s) 1228, 1780
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 136
HinfI GANTC 9 cut(s) 110, 221, 272, 832, 1079, 1329, 1482, 1558, 1804
HphI GGTGA 4 cut(s) 173, 689, 698, 977
Hpy166II GTNNAC 5 cut(s) 63, 109, 352, 1265, 1516
Hpy188I TCNGA 9 cut(s) 19, 172, 697, 876, 978, 1272, 1628, 1695, 1829
Hpy8I GTNNAC 5 cut(s) 63, 109, 352, 1265, 1516
HpyAV CCTTC 6 cut(s) 96, 811, 841, 1319, 1618, 1936
HpyCH4III ACNGT 5 cut(s) 911, 1507, 1520, 1534, 1847
HpyCH4IV ACGT 2 cut(s) 10, 1729
HpyF10VI GCNNNNNNNGC 7 cut(s) 260, 724, 790, 934, 943, 1106, 1973
HpyF3I CTNAG 9 cut(s) 66, 98, 147, 267, 413, 932, 975, 1170, 1612
HpySE526I ACGT 2 cut(s) 10, 1729
KpnI GGTACC 1 cut(s) 1891
Ksp22I TGATCA 2 cut(s) 37, 340
Kzo9I GATC 9 cut(s) 4, 37, 82, 94, 340, 745, 1137, 1592, 1690
LguI GCTCTTC 3 cut(s) 261, 876, 1011
LmnI GCTCC 2 cut(s) 391, 764
Lsp1109I GCAGC 5 cut(s) 793, 958, 1060, 1821, 1917
LweI GCATC 2 cut(s) 1354, 1917
MabI ACCWGGT 2 cut(s) 195, 1258
MaeI CTAG 4 cut(s) 446, 477, 921, 1983
MaeII ACGT 2 cut(s) 10, 1729
MaeIII GTNAC 2 cut(s) 550, 647
MalI GATC 9 cut(s) 6, 39, 84, 96, 342, 747, 1139, 1594, 1692
MboI GATC 9 cut(s) 4, 37, 82, 94, 340, 745, 1137, 1592, 1690
MflI RGATCY 3 cut(s) 4, 94, 1592
MhlI GDGCHC 3 cut(s) 183, 871, 1978
MlyI GAGTC 3 cut(s) 104, 281, 1338
MmeI TCCRAC 2 cut(s) 781, 1647
MseI TTAA 9 cut(s) 123, 207, 441, 1052, 1160, 1374, 1437, 1544, 1587
MslI CAYNNNNRTG 1 cut(s) 582
MspA1I CMGCKG 2 cut(s) 1073, 1834
MspR9I CCNGG 2 cut(s) 197, 1260
MvaI CCWGG 2 cut(s) 197, 1260
MwoI GCNNNNNNNGC 7 cut(s) 260, 724, 790, 934, 943, 1106, 1973
NcoI CCATGG 1 cut(s) 1393
NdeII GATC 9 cut(s) 4, 37, 82, 94, 340, 745, 1137, 1592, 1690
NlaIV GGNNCC 4 cut(s) 6, 1594, 1889, 1977
NspV TTCGAA 1 cut(s) 1807
PagI TCATGA 3 cut(s) 280, 1122, 1854
PciSI GCTCTTC 3 cut(s) 261, 876, 1011
PfeI GAWTC 6 cut(s) 221, 832, 1079, 1482, 1558, 1804
PflMI CCANNNNNTGG 1 cut(s) 1410
PkrI GCNGC 7 cut(s) 783, 827, 948, 1075, 1836, 1922, 1932
PleI GAGTC 3 cut(s) 104, 280, 1337
PpsI GAGTC 3 cut(s) 104, 280, 1337
Psp6I CCWGG 2 cut(s) 195, 1258
PspFI CCCAGC 1 cut(s) 1820
PspGI CCWGG 2 cut(s) 195, 1258
PspN4I GGNNCC 4 cut(s) 6, 1594, 1889, 1977
PspPI GGNCC 2 cut(s) 1265, 1843
PstI CTGCAG 1 cut(s) 783
PstNI CAGNNNCTG 1 cut(s) 893
PsuI RGATCY 3 cut(s) 4, 94, 1592
PvuII CAGCTG 2 cut(s) 1073, 1834
RsaI GTAC 8 cut(s) 71, 188, 305, 353, 973, 1391, 1490, 1889
RsaNI GTAC 8 cut(s) 70, 187, 304, 352, 972, 1390, 1489, 1888
RseI CAYNNNNRTG 1 cut(s) 582
SapI GCTCTTC 3 cut(s) 261, 876, 1011
SaqAI TTAA 9 cut(s) 123, 207, 441, 1052, 1160, 1374, 1437, 1544, 1587
SatI GCNGC 7 cut(s) 782, 826, 947, 1074, 1835, 1921, 1931
Sau3AI GATC 9 cut(s) 4, 37, 82, 94, 340, 745, 1137, 1592, 1690
Sau96I GGNCC 2 cut(s) 1265, 1843
SchI GAGTC 3 cut(s) 104, 281, 1338
ScrFI CCNGG 2 cut(s) 197, 1260
SduI GDGCHC 3 cut(s) 183, 871, 1978
SexAI ACCWGGT 2 cut(s) 195, 1258
SfaNI GCATC 2 cut(s) 1354, 1917
SfcI CTRYAG 1 cut(s) 779
SfuI TTCGAA 1 cut(s) 1807
SinI GGWCC 2 cut(s) 1265, 1843
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 4 cut(s) 90, 182, 1249, 1303
SmoI CTYRAG 4 cut(s) 90, 182, 1249, 1303
SsiI CCGC 5 cut(s) 497, 524, 825, 1015, 1920
SspMI CTAG 4 cut(s) 446, 477, 921, 1983
StyD4I CCNGG 2 cut(s) 195, 1258
StyI CCWWGG 3 cut(s) 445, 1393, 1523
TaaI ACNGT 5 cut(s) 911, 1507, 1520, 1534, 1847
TaiI ACGT 2 cut(s) 13, 1732
TaqI TCGA 2 cut(s) 1315, 1807
TatI WGTACW 2 cut(s) 186, 971
TauI GCSGC 2 cut(s) 828, 1923
TfiI GAWTC 6 cut(s) 221, 832, 1079, 1482, 1558, 1804
Tru1I TTAA 9 cut(s) 123, 207, 441, 1052, 1160, 1374, 1437, 1544, 1587
Tru9I TTAA 9 cut(s) 123, 207, 441, 1052, 1160, 1374, 1437, 1544, 1587
TscAI CASTG 1 cut(s) 271
TseI GCWGC 5 cut(s) 781, 946, 1073, 1834, 1930
TspGWI ACGGA 2 cut(s) 369, 1199
TspRI CASTG 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 1410
VpaK11BI GGWCC 2 cut(s) 1265, 1843
XagI CCTNNNNNAGG 1 cut(s) 782
XapI RAATTY 4 cut(s) 20, 1540, 1552, 1770
XmaJI CCTAGG 1 cut(s) 445
XspI CTAG 4 cut(s) 446, 477, 921, 1983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.