RLG00000035792

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000624
Physical Location & Seq
Forward (+)
71659277 .. 71661080
1804 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035792

Sequence Viewer

Length: 1692 bp
ATGGATCCACGTTTCTCTGAATTTCCCAATTTCAATGATCAAACCCTTTTGCCTGATATGTTGAATGAGTACCCAAGTAGTCACCCTTTTGAAGGTCTTAGCTTTTTGGACCCAAATCCCAGTAACTATGCTCTATCACCAAGCTTTACGCGTGAGGGAGATGACGATGAGTTTTCTGGTAGTGTGCTCAAGTACATAAACCAGGTGCTTATGGAGGAGGACATGGAGTCAAAGCCATGTATGTTCCATGACCCTTTGGCTCTTCAAGCCACTGAGGAGTCTCTTTATGAGGTTATTACTGGGAAGTACCCAACTTCCCAAGACCAAAGCCCTCTTTGTGCTGATCAAAATGTGCATGGAAATGGAAATGGGAATGGGATGGTGGGTTGTGAGTTTATGGTTCCTGATGTAATTTGTAAGAATGAATTGTTAGTACAATTTAACCAAGGGGCAGAGGAGGGTAGTAAGTTTCTTCCTAGAGGTCAGTTGATTAGTGCGGAGGGCAACAAGCCATGTGCTGTGGCGGATAGAGAGGCTGGAGATGAGGTAGTTACGACCCAGAAGAATGGGAGCGAGCATTTTCGTGTTGGTTCTAGGGGAAAGAAAAATCATCAAAGGGAAGATATAGATTTGGAGGATGGCAGGAGTAACAAGCAATCGGCGGTTTATATGGATGATGAGGAGGGTGAGCTGTCTGATATTTTTGACAAGGTGTTGCTCTCCAAGCCTATCAAGAGTAAAGACGATCAAGTCAGTCAGAATGGAGCATACAATGGCCTGCAGCAGGATGGGCTATCTGTTGGAATTGGTAATGGGAAGGTTTGCAGCAAGAATCAAGGCAACAAGAAGGAAGTGATAGATTTGAGGGCTCTTCTGATTTCATGTGCAGAAGCTGTATCTGTTGATGATCACGAGACTGCTAGTGAGCTACTAAAGCAGATTATACAGCACTCTTCCCCATTTGGTGATTATACTCAGAGGTTGGCTCAGTGCTTTGCAAATGCTCTTGAAGCACGGTTGGCTGGCACCGGAACTCAAATATATACTGCTTTATCTACTAAACGAATGTCAGCTGCTGATATGCTGAAGTTTTACCAAGCTTATATTGCAGTCTGCCCGTTCAAGAAGATGTTGATGATCTTTGCAAACCATATGATTTTAAAAGCTGCTGAGAAAGCAGAAACACTTCATATAATAGATTTTGGTATCCGTTATGGTTTTCAATGGCCTGCTCTCATTCATTGCCTCTCAAGAAGACCTGGTGGACCTCCTAAATTACGTATTACAGGGCCAAAAAAATGGGAAACTCTCCAAGTTGATGACCTTAAAGTTCAAAGAAACGAGGTGCTTGCTGTTAATTGTCTGTGCCGATTTAAGTACCTCCTTGATGAGACTGTTGTGGTAAACAGTCCAAGGGATACTGTTCTAAATTTAATTAGGAATTTGAATCCTGATGTCTTTGTCCAAAGTGTTGTTAATGGATCCCATCAGGCACCCTTCTTTGTTCCACGCTTCCGAGAGGCACTATTCCACTTCTCTGCATTGTTTGATATGTTGGATAACAATTTGCCTCCTGACGATCAGATGAGATTGGGGTTTGAGAAAGAGTTCATTGGACGTGAAGTTGTGAATATAGTAGCTTGTGAGGGCTCTCAAAGAATTGTTAGGCCAGAAACATACAAGCAGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

564

Amino Acids

63.06

Weight (kDa)

5.11

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 288 - 430 4.7e-47 GRAS domain family
GRAS PF03514 432 - 563 1.3e-37 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 749
AccB1I GGYRCC 2 cut(s) 1025, 1492
AccII CGCG 1 cut(s) 151
AciI CCGC 3 cut(s) 497, 524, 662
AclWI GGATC 3 cut(s) 12, 1476, 1489
AcsI RAATTY 3 cut(s) 20, 1429, 1441
AcuI CTGAAG 1 cut(s) 1106
AfaI GTAC 5 cut(s) 71, 194, 308, 435, 1379
AfiI CCNNNNNNNGG 2 cut(s) 92, 784
AflIII ACRYGT 1 cut(s) 149
AgsI TTSAA 9 cut(s) 34, 64, 92, 266, 1010, 1123, 1223, 1334, 1447
AhdI GACNNNNNGTC 1 cut(s) 226
AjiI CACGTC 1 cut(s) 1619
AjnI CCWGG 2 cut(s) 201, 1258
AluBI AGCT 9 cut(s) 102, 144, 691, 893, 928, 1073, 1100, 1166, 1640
AluI AGCT 9 cut(s) 102, 144, 691, 893, 928, 1073, 1100, 1166, 1640
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 3 cut(s) 285, 908, 1385
AlwI GGATC 3 cut(s) 12, 1476, 1489
AlwNI CAGNNNCTG 2 cut(s) 893, 1076
AoxI GGCC 4 cut(s) 775, 1226, 1289, 1667
ApeKI GCWGC 4 cut(s) 781, 825, 1073, 1166
ApoI RAATTY 3 cut(s) 20, 1429, 1441
Asp700I GAANNNNTTC 2 cut(s) 1185, 1607
AspS9I GGNCC 3 cut(s) 109, 1265, 1289
AsuHPI GGTGA 4 cut(s) 74, 129, 698, 977
AvaII GGWCC 2 cut(s) 109, 1265
BamHI GGATCC 2 cut(s) 4, 1481
BanI GGYRCC 2 cut(s) 1025, 1492
BanII GRGCYC 2 cut(s) 871, 1652
BauI CACGAG 1 cut(s) 911
BbsI GAAGAC 1 cut(s) 1261
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 4 cut(s) 793, 837, 1060, 1153
BccI CCATC 4 cut(s) 373, 632, 782, 1494
BcgI CGANNNNNNTGC 2 cut(s) 1331, 1365
BciT130I CCWGG 2 cut(s) 203, 1260
BciVI GTATCC 2 cut(s) 1217, 1411
BclI TGATCA 3 cut(s) 37, 343, 907
BcoDI GTCTC 3 cut(s) 285, 908, 1385
BfaI CTAG 3 cut(s) 477, 594, 921
BfmI CTRYAG 1 cut(s) 779
BfuI GTATCC 2 cut(s) 1217, 1411
BisI GCNGC 4 cut(s) 782, 826, 1074, 1167
BlsI GCNGC 4 cut(s) 783, 827, 1075, 1168
Bme1390I CCNGG 2 cut(s) 203, 1260
Bme18I GGWCC 2 cut(s) 109, 1265
BmeRI GACNNNNNGTC 1 cut(s) 226
BmgBI CACGTC 1 cut(s) 1619
BmgT120I GGNCC 3 cut(s) 109, 1265, 1289
BmiI GGNNCC 6 cut(s) 6, 111, 402, 1027, 1483, 1494
BmrFI CCNGG 2 cut(s) 203, 1260
BmrI ACTGGG 2 cut(s) 114, 309
BmuI ACTGGG 2 cut(s) 114, 309
BpiI GAAGAC 1 cut(s) 1261
BplI GAGNNNNNCTC 2 cut(s) 970, 1002
BpmI CTGGAG 1 cut(s) 558
BpuEI CTTGAG 2 cut(s) 173, 1234
BsaAI YACGTR 1 cut(s) 1280
BsaJI CCNNGG 2 cut(s) 445, 1412
BsaWI WCCGGW 1 cut(s) 1028
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 784
Bse1I ACTGG 2 cut(s) 120, 304
Bse3DI GCAATG 1 cut(s) 1240
BseBI CCWGG 2 cut(s) 203, 1260
BseDI CCNNGG 2 cut(s) 445, 1412
BseGI GGATG 4 cut(s) 384, 643, 679, 793
BseLI CCNNNNNNNGG 2 cut(s) 92, 784
BseMI GCAATG 1 cut(s) 1240
BseMII CTCAG 4 cut(s) 264, 989, 1001, 1161
BseNI ACTGG 2 cut(s) 120, 304
BseRI GAGGAG 4 cut(s) 230, 290, 470, 695
BseXI GCAGC 4 cut(s) 793, 837, 1060, 1153
BsgI GTGCAG 1 cut(s) 906
Bsh1236I CGCG 1 cut(s) 151
BshFI GGCC 4 cut(s) 777, 1228, 1291, 1669
BshNI GGYRCC 2 cut(s) 1025, 1492
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 1 cut(s) 1029
BslI CCNNNNNNNGG 2 cut(s) 92, 784
BsmAI GTCTC 3 cut(s) 285, 908, 1385
BsnI GGCC 4 cut(s) 777, 1228, 1291, 1669
Bsp1286I GDGCHC 3 cut(s) 189, 871, 1652
Bsp143I GATC 8 cut(s) 4, 37, 343, 745, 907, 1137, 1481, 1579
BspACI CCGC 3 cut(s) 497, 524, 662
BspANI GGCC 4 cut(s) 777, 1228, 1291, 1669
BspCNI CTCAG 4 cut(s) 265, 988, 1000, 1162
BspFNI CGCG 1 cut(s) 151
BspLI GGNNCC 6 cut(s) 6, 111, 402, 1027, 1483, 1494
BspMAI CTGCAG 1 cut(s) 783
BspPI GGATC 3 cut(s) 12, 1476, 1489
BspQI GCTCTTC 2 cut(s) 267, 876
BspT107I GGYRCC 2 cut(s) 1025, 1492
BsrDI GCAATG 1 cut(s) 1240
BsrI ACTGG 2 cut(s) 120, 304
BssECI CCNNGG 2 cut(s) 445, 1412
BssMI GATC 8 cut(s) 4, 37, 343, 745, 907, 1137, 1481, 1579
BssSI CACGAG 1 cut(s) 911
BssT1I CCWWGG 2 cut(s) 445, 1412
Bst2BI CACGAG 1 cut(s) 911
Bst2UI CCWGG 2 cut(s) 203, 1260
Bst4CI ACNGT 4 cut(s) 1017, 1396, 1409, 1423
Bst6I CTCTTC 3 cut(s) 267, 876, 958
BstBAI YACGTR 1 cut(s) 1280
BstC8I GCNNGC 5 cut(s) 575, 779, 1024, 1230, 1350
BstDEI CTNAG 5 cut(s) 98, 273, 975, 987, 1170
BstENI CCTNNNNNAGG 2 cut(s) 90, 782
BstF5I GGATG 4 cut(s) 384, 643, 679, 793
BstFNI CGCG 1 cut(s) 151
BstKTI GATC 8 cut(s) 7, 40, 346, 748, 910, 1140, 1484, 1582
BstMAI GTCTC 3 cut(s) 285, 908, 1385
BstMBI GATC 8 cut(s) 4, 37, 343, 745, 907, 1137, 1481, 1579
BstMWI GCNNNNNNNGC 8 cut(s) 266, 724, 790, 934, 1010, 1019, 1106, 1175
BstNI CCWGG 2 cut(s) 203, 1260
BstSCI CCNGG 2 cut(s) 201, 1258
BstSFI CTRYAG 1 cut(s) 779
BstSNI TACGTA 1 cut(s) 1280
BstUI CGCG 1 cut(s) 151
BstV1I GCAGC 4 cut(s) 793, 837, 1060, 1153
BstV2I GAAGAC 1 cut(s) 1261
BstX2I RGATCY 2 cut(s) 4, 1481
BstXI CCANNNNNNTGG 2 cut(s) 566, 1299
BstYI RGATCY 2 cut(s) 4, 1481
BsuI GTATCC 2 cut(s) 1217, 1411
BsuRI GGCC 4 cut(s) 777, 1228, 1291, 1669
BtrI CACGTC 1 cut(s) 1619
BtsCI GGATG 4 cut(s) 384, 643, 679, 793
BtsI GCAGTG 1 cut(s) 1691
BtsIMutI CAGTG 3 cut(s) 270, 995, 1691
Cac8I GCNNGC 5 cut(s) 575, 779, 1024, 1230, 1350
CaiI CAGNNNCTG 2 cut(s) 893, 1076
Cfr13I GGNCC 3 cut(s) 109, 1265, 1289
CsiI ACCWGGT 2 cut(s) 201, 1258
Csp6I GTAC 5 cut(s) 70, 193, 307, 434, 1378
CviAII CATG 6 cut(s) 223, 237, 248, 356, 513, 882
CviQI GTAC 5 cut(s) 70, 193, 307, 434, 1378
DdeI CTNAG 5 cut(s) 98, 273, 975, 987, 1170
DpnI GATC 8 cut(s) 6, 39, 345, 747, 909, 1139, 1483, 1581
DpnII GATC 8 cut(s) 4, 37, 343, 745, 907, 1137, 1481, 1579
DraI TTTAAA 1 cut(s) 1161
DrdI GACNNNNNNGTC 1 cut(s) 749
DriI GACNNNNNGTC 1 cut(s) 226
DseDI GACNNNNNNGTC 1 cut(s) 749
Eam1104I CTCTTC 3 cut(s) 267, 876, 958
Eam1105I GACNNNNNGTC 1 cut(s) 226
EarI CTCTTC 3 cut(s) 267, 876, 958
EciI GGCGGA 1 cut(s) 539
Eco105I TACGTA 1 cut(s) 1280
Eco130I CCWWGG 2 cut(s) 445, 1412
Eco24I GRGCYC 2 cut(s) 871, 1652
Eco47I GGWCC 2 cut(s) 109, 1265
Eco57I CTGAAG 1 cut(s) 1106
EcoNI CCTNNNNNAGG 2 cut(s) 90, 782
EcoRII CCWGG 2 cut(s) 201, 1258
EcoT14I CCWWGG 2 cut(s) 445, 1412
EcoT38I GRGCYC 2 cut(s) 871, 1652
ErhI CCWWGG 2 cut(s) 445, 1412
FaeI CATG 6 cut(s) 226, 240, 251, 359, 516, 885
FatI CATG 6 cut(s) 222, 236, 247, 355, 512, 881
FauNDI CATATG 1 cut(s) 1152
FbaI TGATCA 3 cut(s) 37, 343, 907
Fnu4HI GCNGC 4 cut(s) 782, 826, 1074, 1167
FokI GGATG 4 cut(s) 391, 650, 686, 800
FriOI GRGCYC 2 cut(s) 871, 1652
Fsp4HI GCNGC 4 cut(s) 782, 826, 1074, 1167
FspBI CTAG 3 cut(s) 477, 594, 921
GluI GCNGC 4 cut(s) 782, 826, 1074, 1167
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 4 cut(s) 777, 1228, 1291, 1669
HapII CCGG 1 cut(s) 1029
Hin1II CATG 6 cut(s) 226, 240, 251, 359, 516, 885
HindIII AAGCTT 2 cut(s) 142, 1098
HinfI GANTC 4 cut(s) 227, 278, 832, 1447
HpaII CCGG 1 cut(s) 1029
HphI GGTGA 4 cut(s) 74, 129, 698, 977
Hpy166II GTNNAC 2 cut(s) 1265, 1405
Hpy188I TCNGA 7 cut(s) 19, 697, 759, 876, 978, 1517, 1584
Hpy188III TCNNGA 8 cut(s) 404, 733, 911, 1007, 1123, 1251, 1451, 1574
Hpy8I GTNNAC 2 cut(s) 1265, 1405
HpyAV CCTTC 4 cut(s) 86, 811, 841, 1507
HpyCH4III ACNGT 4 cut(s) 1017, 1396, 1409, 1423
HpyCH4IV ACGT 3 cut(s) 10, 1279, 1618
HpyCH4V TGCA 8 cut(s) 355, 781, 825, 887, 998, 1109, 1145, 1541
HpyF10VI GCNNNNNNNGC 8 cut(s) 266, 724, 790, 934, 1010, 1019, 1106, 1175
HpyF3I CTNAG 5 cut(s) 98, 273, 975, 987, 1170
HpySE526I ACGT 3 cut(s) 10, 1279, 1618
Hsp92II CATG 6 cut(s) 226, 240, 251, 359, 516, 885
Ksp22I TGATCA 3 cut(s) 37, 343, 907
Kzo9I GATC 8 cut(s) 4, 37, 343, 745, 907, 1137, 1481, 1579
LguI GCTCTTC 2 cut(s) 267, 876
LmnI GCTCC 2 cut(s) 570, 764
Lsp1109I GCAGC 4 cut(s) 793, 837, 1060, 1153
MabI ACCWGGT 2 cut(s) 201, 1258
MaeI CTAG 3 cut(s) 477, 594, 921
MaeII ACGT 3 cut(s) 10, 1279, 1618
MaeIII GTNAC 4 cut(s) 80, 122, 550, 647
MalI GATC 8 cut(s) 6, 39, 345, 747, 909, 1139, 1483, 1581
MboI GATC 8 cut(s) 4, 37, 343, 745, 907, 1137, 1481, 1579
MboII GAAGA 8 cut(s) 254, 464, 574, 632, 863, 945, 1138, 1266
MflI RGATCY 2 cut(s) 4, 1481
MhlI GDGCHC 3 cut(s) 189, 871, 1652
MluI ACGCGT 1 cut(s) 149
MlyI GAGTC 2 cut(s) 236, 287
MmeI TCCRAC 2 cut(s) 781, 1536
MroXI GAANNNNTTC 2 cut(s) 1185, 1607
MseI TTAA 7 cut(s) 441, 1160, 1326, 1356, 1374, 1433, 1476
MslI CAYNNNNRTG 2 cut(s) 360, 582
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 1 cut(s) 1029
MspR9I CCNGG 2 cut(s) 203, 1260
MvaI CCWGG 2 cut(s) 203, 1260
MvnI CGCG 1 cut(s) 151
MwoI GCNNNNNNNGC 8 cut(s) 266, 724, 790, 934, 1010, 1019, 1106, 1175
NdeI CATATG 1 cut(s) 1152
NdeII GATC 8 cut(s) 4, 37, 343, 745, 907, 1137, 1481, 1579
NlaIII CATG 6 cut(s) 226, 240, 251, 359, 516, 885
NlaIV GGNNCC 6 cut(s) 6, 111, 402, 1027, 1483, 1494
NmuCI GTSAC 1 cut(s) 80
PciSI GCTCTTC 2 cut(s) 267, 876
PdmI GAANNNNTTC 2 cut(s) 1185, 1607
PfeI GAWTC 2 cut(s) 832, 1447
PkrI GCNGC 4 cut(s) 783, 827, 1075, 1168
PleI GAGTC 2 cut(s) 235, 286
PpsI GAGTC 2 cut(s) 235, 286
Ppu21I YACGTR 1 cut(s) 1280
Psp6I CCWGG 2 cut(s) 201, 1258
PspGI CCWGG 2 cut(s) 201, 1258
PspN4I GGNNCC 6 cut(s) 6, 111, 402, 1027, 1483, 1494
PspPI GGNCC 3 cut(s) 109, 1265, 1289
PstI CTGCAG 1 cut(s) 783
PstNI CAGNNNCTG 2 cut(s) 893, 1076
PsuI RGATCY 2 cut(s) 4, 1481
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 5 cut(s) 71, 194, 308, 435, 1379
RsaNI GTAC 5 cut(s) 70, 193, 307, 434, 1378
RseI CAYNNNNRTG 2 cut(s) 360, 582
SapI GCTCTTC 2 cut(s) 267, 876
SaqAI TTAA 7 cut(s) 441, 1160, 1326, 1356, 1374, 1433, 1476
SatI GCNGC 4 cut(s) 782, 826, 1074, 1167
Sau3AI GATC 8 cut(s) 4, 37, 343, 745, 907, 1137, 1481, 1579
Sau96I GGNCC 3 cut(s) 109, 1265, 1289
SchI GAGTC 2 cut(s) 236, 287
ScrFI CCNGG 2 cut(s) 203, 1260
SduI GDGCHC 3 cut(s) 189, 871, 1652
SexAI ACCWGGT 2 cut(s) 201, 1258
SfcI CTRYAG 1 cut(s) 779
SinI GGWCC 2 cut(s) 109, 1265
SmiMI CAYNNNNRTG 2 cut(s) 360, 582
SmlI CTYRAG 2 cut(s) 188, 1249
SmoI CTYRAG 2 cut(s) 188, 1249
SnaBI TACGTA 1 cut(s) 1280
SsiI CCGC 3 cut(s) 497, 524, 662
SspMI CTAG 3 cut(s) 477, 594, 921
StyD4I CCNGG 2 cut(s) 201, 1258
StyI CCWWGG 2 cut(s) 445, 1412
TaaI ACNGT 4 cut(s) 1017, 1396, 1409, 1423
TaiI ACGT 3 cut(s) 13, 1282, 1621
TatI WGTACW 2 cut(s) 192, 433
TfiI GAWTC 2 cut(s) 832, 1447
Tru1I TTAA 7 cut(s) 441, 1160, 1326, 1356, 1374, 1433, 1476
Tru9I TTAA 7 cut(s) 441, 1160, 1326, 1356, 1374, 1433, 1476
TscAI CASTG 3 cut(s) 277, 995, 1691
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 4 cut(s) 781, 825, 1073, 1166
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 5 cut(s) 438, 870, 1178, 1229, 1600
TspGWI ACGGA 1 cut(s) 1199
TspRI CASTG 3 cut(s) 277, 995, 1691
VpaK11BI GGWCC 2 cut(s) 109, 1265
XagI CCTNNNNNAGG 2 cut(s) 90, 782
XapI RAATTY 3 cut(s) 20, 1429, 1441
XmnI GAANNNNTTC 2 cut(s) 1185, 1607
XspI CTAG 3 cut(s) 477, 594, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.