Rmu_sc0001010.1_g000086

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001010.1
OG0000624
Physical Location & Seq
Reverse (-)
313625 .. 315610
1986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001010.1_g000086.1.cds

Sequence Viewer

Length: 1905 bp
atggatccacgtttctctgaatttcccaatttcaatgatcaaacccttttgcctgatatgttgaatgagtacccaagtagtgacccttttgaagatctcagctttttggacccaaaccccagtaactatgctctatcatctagctttagccctgagggagatgatggtgagttttctgacagtgtgctcaactacataaacaatgtgcttatggaggaggacatggaatcaaaaccctgtatgttccatgaccctttggctcttcaagccactgagaagtctctttatgaggttattggagggaagtacccaccttcccaagaccaaaaccgtgctgatcaaaatgtgcatggaaatgggaatgggaatgggatggtgggttgtgagcttatggttcctgatgtaatttgtaagaatgaattgttagtgcaatttaaccgaggggcagaggagggtagtaagtttcttcctagacatcaattgattagtgcggagggtatcaagccttatgctgtggcggatagaaaggccggagatgtggtagttacgacccagaagaatgggagtgagcattttcgtgttggttccagaggaaagaaaaatcatcaaagggaggatatagatttggaggatgggaggagcaacaagcaatcggcggtttatatggatggtgaggagggtgagctgtctgatatttttgataaggtgttgctctgcaagcctatcatgagtaaagacgatatattcagtcagaatggagcaaacaatggcttgcagcaggatgggctatctgttggaattgaagctctatctgttgatgaccgcgagactgctagtgagctactaaagcagattatgcagcactcttccccatttggtgattgtactcagaggttggctcattgctttgcaaatgctcttgaagcgcggttggctggcacaggaactcaaatatatactgctttaactactaaacgaatgtcagctgctgattcgctgaagttttaccaagcttatattgcagtctgcccgttcatgaagttgttgatgatctttgcaaacgatatgattttaaaagctgctaagaaagcagaaacacttcatataatagattttggtatcctttatggttttcaatggcctgctctcattcattgcctctcaagaagacctggtggacctccgaaattacgcattacaggggtagagcttccctctagtggttttcgaccagaagaaggagtccaagagacagggcatcgcttggcaaagtattgtaggcgatttaatgttcattttgagtactatggcatagccaaaaaatgggaaactctccaatttgatgaccttagagttcaaagaaatgaggtgcttgctgttaattgtctctgccgatttaagtacctccttgatgagacagttgtggcaaacagtccaagagatactgttctaaatttaattaggaagttgaatcctgatgtctttgtccaaagtgttgttaatggatcccatcatgcacccttctttgttccacgcttccgacaggcactgttccacttctctgcattgtttgatatgttggataacaatttgcctcctgacgatcagatgagattggggtttgagaaagagtttattggacgtgaagttgtgaatatagtagcttgtgagggctctcaaagaattgttaggccagaaacatttaagcagtggcagattcgaaacatgagggctgggttcagacagctaccattggaccgtgaagtcatgaacaaaataaaggataaggtaaaaatggggtaccacccttattttgttgttgatgaagatggccactggatgctgcagggatggaagggcagaattatgtttgcttctgcctgttgggcaccatctaggacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

634

Amino Acids

71.77

Weight (kDa)

5.67

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1763
Acc65I GGTACC 1 cut(s) 1800
AccB1I GGYRCC 2 cut(s) 1800, 1888
AccB7I CCANNNNNTGG 1 cut(s) 1323
AccII CGCG 2 cut(s) 825, 928
AciI CCGC 5 cut(s) 491, 518, 656, 823, 928
AclWI GGATC 3 cut(s) 12, 1500, 1513
AcoI YGGCCR 1 cut(s) 1831
AcsI RAATTY 2 cut(s) 20, 1453
AcuI CTGAAG 1 cut(s) 1019
AfaI GTAC 6 cut(s) 71, 308, 886, 1304, 1403, 1802
AfiI CCNNNNNNNGG 3 cut(s) 1220, 1238, 1323
AgsI TTSAA 9 cut(s) 34, 64, 92, 266, 803, 923, 1136, 1358, 1471
AjiI CACGTC 1 cut(s) 1643
AjnI CCWGG 1 cut(s) 1171
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 5 cut(s) 285, 821, 1244, 1391, 1409
AlwI GGATC 3 cut(s) 12, 1500, 1513
AlwNI CAGNNNCTG 2 cut(s) 989, 1549
AoxI GGCC 4 cut(s) 528, 1139, 1691, 1831
ApeKI GCWGC 5 cut(s) 775, 859, 986, 1079, 1843
ApoI RAATTY 2 cut(s) 20, 1453
Asp700I GAANNNNTTC 1 cut(s) 1098
Asp718I GGTACC 1 cut(s) 1800
AspLEI GCGC 1 cut(s) 928
AspS9I GGNCC 3 cut(s) 109, 1178, 1756
AsuHPI GGTGA 4 cut(s) 179, 683, 692, 890
AsuII TTCGAA 1 cut(s) 1720
AvaII GGWCC 3 cut(s) 109, 1178, 1756
AxyI CCTNAGG 1 cut(s) 153
BaeGI GKGCMC 1 cut(s) 1891
BalI TGGCCA 1 cut(s) 1833
BamHI GGATCC 2 cut(s) 4, 1505
BanI GGYRCC 2 cut(s) 1800, 1888
BanII GRGCYC 1 cut(s) 1676
BbsI GAAGAC 1 cut(s) 1174
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 5 cut(s) 787, 871, 973, 1066, 1830
BccI CCATC 9 cut(s) 158, 367, 626, 662, 776, 1518, 1823, 1845, 1900
BciT130I CCWGG 1 cut(s) 1173
BciVI GTATCC 1 cut(s) 1130
BclI TGATCA 2 cut(s) 37, 337
BcoDI GTCTC 5 cut(s) 285, 821, 1244, 1391, 1409
BfaI CTAG 5 cut(s) 141, 471, 834, 1218, 1896
BfmI CTRYAG 1 cut(s) 1844
BfuI GTATCC 1 cut(s) 1130
BglI GCCNNNNNGGC 1 cut(s) 1886
BglII AGATCT 1 cut(s) 94
BisI GCNGC 5 cut(s) 776, 860, 987, 1080, 1844
BlsI GCNGC 5 cut(s) 777, 861, 988, 1081, 1845
BmcAI AGTACT 1 cut(s) 1304
Bme1390I CCNGG 1 cut(s) 1173
Bme18I GGWCC 3 cut(s) 109, 1178, 1756
BmgBI CACGTC 1 cut(s) 1643
BmgT120I GGNCC 3 cut(s) 109, 1178, 1756
BmiI GGNNCC 7 cut(s) 6, 111, 396, 586, 1507, 1802, 1890
BmrFI CCNGG 1 cut(s) 1173
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 2 cut(s) 1267, 1830
BmuI ACTGGG 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 1174
BplI GAGNNNNNCTC 4 cut(s) 883, 915, 1199, 1231
Bpu14I TTCGAA 1 cut(s) 1720
BpuEI CTTGAG 1 cut(s) 1147
BsaJI CCNNGG 1 cut(s) 439
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bsc4I CCNNNNNNNGG 3 cut(s) 1220, 1238, 1323
Bse1I ACTGG 2 cut(s) 120, 1841
Bse21I CCTNAGG 1 cut(s) 153
Bse3DI GCAATG 2 cut(s) 901, 1153
BseBI CCWGG 1 cut(s) 1173
BseDI CCNNGG 1 cut(s) 439
BseGI GGATG 6 cut(s) 378, 637, 673, 787, 1845, 1856
BseLI CCNNNNNNNGG 3 cut(s) 1220, 1238, 1323
BseMI GCAATG 2 cut(s) 901, 1153
BseMII CTCAG 4 cut(s) 112, 144, 264, 902
BseNI ACTGG 2 cut(s) 120, 1841
BseRI GAGGAG 4 cut(s) 230, 464, 652, 689
BseSI GKGCMC 1 cut(s) 1891
BseXI GCAGC 5 cut(s) 787, 871, 973, 1066, 1830
BseYI CCCAGC 1 cut(s) 1733
Bsh1236I CGCG 2 cut(s) 825, 928
BshFI GGCC 4 cut(s) 530, 1141, 1693, 1833
BshNI GGYRCC 2 cut(s) 1800, 1888
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 1 cut(s) 531
BslI CCNNNNNNNGG 3 cut(s) 1220, 1238, 1323
BsmAI GTCTC 5 cut(s) 285, 821, 1244, 1391, 1409
BsnI GGCC 4 cut(s) 530, 1141, 1693, 1833
Bsp119I TTCGAA 1 cut(s) 1720
Bsp1286I GDGCHC 3 cut(s) 189, 1676, 1891
Bsp143I GATC 7 cut(s) 4, 37, 94, 337, 1050, 1505, 1603
BspACI CCGC 5 cut(s) 491, 518, 656, 823, 928
BspANI GGCC 4 cut(s) 530, 1141, 1693, 1833
BspCNI CTCAG 4 cut(s) 111, 145, 265, 901
BspFNI CGCG 2 cut(s) 825, 928
BspHI TCATGA 3 cut(s) 726, 1035, 1767
BspLI GGNNCC 7 cut(s) 6, 111, 396, 586, 1507, 1802, 1890
BspMAI CTGCAG 1 cut(s) 1848
BspPI GGATC 3 cut(s) 12, 1500, 1513
BspQI GCTCTTC 1 cut(s) 267
BspT104I TTCGAA 1 cut(s) 1720
BspT107I GGYRCC 2 cut(s) 1800, 1888
BsrDI GCAATG 2 cut(s) 901, 1153
BsrI ACTGG 2 cut(s) 120, 1841
BssECI CCNNGG 1 cut(s) 439
BssMI GATC 7 cut(s) 4, 37, 94, 337, 1050, 1505, 1603
Bst2UI CCWGG 1 cut(s) 1173
Bst4CI ACNGT 7 cut(s) 182, 332, 1420, 1433, 1447, 1551, 1760
Bst6I CTCTTC 2 cut(s) 267, 871
BstAPI GCANNNNNTGC 1 cut(s) 856
BstBI TTCGAA 1 cut(s) 1720
BstC8I GCNNGC 5 cut(s) 719, 773, 937, 1143, 1374
BstDEI CTNAG 6 cut(s) 98, 153, 273, 888, 1083, 1349
BstF5I GGATG 6 cut(s) 378, 637, 673, 787, 1845, 1856
BstFNI CGCG 2 cut(s) 825, 928
BstHHI GCGC 1 cut(s) 928
BstKTI GATC 7 cut(s) 7, 40, 97, 340, 1053, 1508, 1606
BstMAI GTCTC 5 cut(s) 285, 821, 1244, 1391, 1409
BstMBI GATC 7 cut(s) 4, 37, 94, 337, 1050, 1505, 1603
BstNI CCWGG 1 cut(s) 1173
BstSCI CCNGG 1 cut(s) 1171
BstSFI CTRYAG 1 cut(s) 1844
BstSLI GKGCMC 1 cut(s) 1891
BstUI CGCG 2 cut(s) 825, 928
BstV1I GCAGC 5 cut(s) 787, 871, 973, 1066, 1830
BstV2I GAAGAC 1 cut(s) 1174
BstX2I RGATCY 3 cut(s) 4, 94, 1505
BstXI CCANNNNNNTGG 1 cut(s) 560
BstYI RGATCY 3 cut(s) 4, 94, 1505
Bsu36I CCTNAGG 1 cut(s) 153
BsuI GTATCC 1 cut(s) 1130
BsuRI GGCC 4 cut(s) 530, 1141, 1693, 1833
BtgZI GCGATG 1 cut(s) 1244
BtrI CACGTC 1 cut(s) 1643
BtsCI GGATG 6 cut(s) 378, 637, 673, 787, 1845, 1856
BtsI GCAGTG 1 cut(s) 1715
BtsIMutI CAGTG 5 cut(s) 187, 270, 1547, 1715, 1834
Cac8I GCNNGC 5 cut(s) 719, 773, 937, 1143, 1374
CaiI CAGNNNCTG 2 cut(s) 989, 1549
CciI TCATGA 3 cut(s) 726, 1035, 1767
CfoI GCGC 1 cut(s) 928
Cfr13I GGNCC 3 cut(s) 109, 1178, 1756
CsiI ACCWGGT 1 cut(s) 1171
Csp6I GTAC 6 cut(s) 70, 307, 885, 1303, 1402, 1801
CviAII CATG 8 cut(s) 223, 248, 350, 727, 1036, 1514, 1726, 1768
CviQI GTAC 6 cut(s) 70, 307, 885, 1303, 1402, 1801
DdeI CTNAG 6 cut(s) 98, 153, 273, 888, 1083, 1349
DpnI GATC 7 cut(s) 6, 39, 96, 339, 1052, 1507, 1605
DpnII GATC 7 cut(s) 4, 37, 94, 337, 1050, 1505, 1603
DraI TTTAAA 1 cut(s) 1074
DrdI GACNNNNNNGTC 1 cut(s) 1763
DseDI GACNNNNNNGTC 1 cut(s) 1763
EaeI YGGCCR 1 cut(s) 1831
Eam1104I CTCTTC 2 cut(s) 267, 871
EarI CTCTTC 2 cut(s) 267, 871
EciI GGCGGA 1 cut(s) 533
Eco24I GRGCYC 1 cut(s) 1676
Eco47I GGWCC 3 cut(s) 109, 1178, 1756
Eco57I CTGAAG 1 cut(s) 1019
Eco81I CCTNAGG 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 1171
EcoT38I GRGCYC 1 cut(s) 1676
FaeI CATG 8 cut(s) 226, 251, 353, 730, 1039, 1517, 1729, 1771
FatI CATG 8 cut(s) 222, 247, 349, 726, 1035, 1513, 1725, 1767
FbaI TGATCA 2 cut(s) 37, 337
Fnu4HI GCNGC 5 cut(s) 776, 860, 987, 1080, 1844
FokI GGATG 6 cut(s) 385, 644, 680, 794, 1852, 1863
FriOI GRGCYC 1 cut(s) 1676
Fsp4HI GCNGC 5 cut(s) 776, 860, 987, 1080, 1844
FspBI CTAG 5 cut(s) 141, 471, 834, 1218, 1896
GlaI GCGC 1 cut(s) 927
GluI GCNGC 5 cut(s) 776, 860, 987, 1080, 1844
GsaI CCCAGC 1 cut(s) 1737
HaeIII GGCC 4 cut(s) 530, 1141, 1693, 1833
HapII CCGG 1 cut(s) 531
HhaI GCGC 1 cut(s) 928
Hin1II CATG 8 cut(s) 226, 251, 353, 730, 1039, 1517, 1729, 1771
Hin6I GCGC 1 cut(s) 926
HinP1I GCGC 1 cut(s) 926
HindIII AAGCTT 1 cut(s) 1011
HinfI GANTC 5 cut(s) 227, 992, 1242, 1471, 1717
HpaII CCGG 1 cut(s) 531
HphI GGTGA 4 cut(s) 179, 683, 692, 890
Hpy166II GTNNAC 1 cut(s) 1178
Hpy188I TCNGA 9 cut(s) 19, 178, 691, 753, 891, 1185, 1541, 1608, 1742
Hpy188III TCNNGA 9 cut(s) 398, 588, 727, 920, 1036, 1164, 1475, 1598, 1768
Hpy8I GTNNAC 1 cut(s) 1178
HpyAV CCTTC 4 cut(s) 324, 1232, 1531, 1849
HpyCH4III ACNGT 7 cut(s) 182, 332, 1420, 1433, 1447, 1551, 1760
HpyCH4IV ACGT 2 cut(s) 10, 1642
HpyF3I CTNAG 6 cut(s) 98, 153, 273, 888, 1083, 1349
HpySE526I ACGT 2 cut(s) 10, 1642
Hsp92II CATG 8 cut(s) 226, 251, 353, 730, 1039, 1517, 1729, 1771
HspAI GCGC 1 cut(s) 926
KpnI GGTACC 1 cut(s) 1804
Ksp22I TGATCA 2 cut(s) 37, 337
Kzo9I GATC 7 cut(s) 4, 37, 94, 337, 1050, 1505, 1603
LguI GCTCTTC 1 cut(s) 267
LmnI GCTCC 2 cut(s) 639, 758
Lsp1109I GCAGC 5 cut(s) 787, 871, 973, 1066, 1830
LweI GCATC 2 cut(s) 1267, 1830
MabI ACCWGGT 1 cut(s) 1171
MaeI CTAG 5 cut(s) 141, 471, 834, 1218, 1896
MaeII ACGT 2 cut(s) 10, 1642
MaeIII GTNAC 3 cut(s) 80, 122, 544
MalI GATC 7 cut(s) 6, 39, 96, 339, 1052, 1507, 1605
MboI GATC 7 cut(s) 4, 37, 94, 337, 1050, 1505, 1603
MboII GAAGA 8 cut(s) 104, 254, 458, 568, 858, 1179, 1247, 1838
MfeI CAATTG 1 cut(s) 479
MflI RGATCY 3 cut(s) 4, 94, 1505
MhlI GDGCHC 3 cut(s) 189, 1676, 1891
MlsI TGGCCA 1 cut(s) 1833
MluNI TGGCCA 1 cut(s) 1833
MlyI GAGTC 1 cut(s) 1251
MmeI TCCRAC 3 cut(s) 775, 1560, 1564
Mox20I TGGCCA 1 cut(s) 1833
MroXI GAANNNNTTC 1 cut(s) 1098
MscI TGGCCA 1 cut(s) 1833
MseI TTAA 9 cut(s) 435, 965, 1073, 1287, 1380, 1398, 1457, 1500, 1704
MslI CAYNNNNRTG 2 cut(s) 354, 576
Msp20I TGGCCA 1 cut(s) 1833
MspA1I CMGCKG 1 cut(s) 986
MspI CCGG 1 cut(s) 531
MspR9I CCNGG 1 cut(s) 1173
MunI CAATTG 1 cut(s) 479
MvaI CCWGG 1 cut(s) 1173
MvnI CGCG 2 cut(s) 825, 928
NdeII GATC 7 cut(s) 4, 37, 94, 337, 1050, 1505, 1603
NlaIII CATG 8 cut(s) 226, 251, 353, 730, 1039, 1517, 1729, 1771
NlaIV GGNNCC 7 cut(s) 6, 111, 396, 586, 1507, 1802, 1890
NmuCI GTSAC 1 cut(s) 80
NspV TTCGAA 1 cut(s) 1720
PagI TCATGA 3 cut(s) 726, 1035, 1767
PciSI GCTCTTC 1 cut(s) 267
PdmI GAANNNNTTC 1 cut(s) 1098
PfeI GAWTC 4 cut(s) 227, 992, 1471, 1717
PflMI CCANNNNNTGG 1 cut(s) 1323
PkrI GCNGC 5 cut(s) 777, 861, 988, 1081, 1845
PleI GAGTC 1 cut(s) 1250
PpsI GAGTC 1 cut(s) 1250
Psp6I CCWGG 1 cut(s) 1171
PspFI CCCAGC 1 cut(s) 1733
PspGI CCWGG 1 cut(s) 1171
PspN4I GGNNCC 7 cut(s) 6, 111, 396, 586, 1507, 1802, 1890
PspPI GGNCC 3 cut(s) 109, 1178, 1756
PstI CTGCAG 1 cut(s) 1848
PstNI CAGNNNCTG 2 cut(s) 989, 1549
PsuI RGATCY 3 cut(s) 4, 94, 1505
PvuII CAGCTG 1 cut(s) 986
RsaI GTAC 6 cut(s) 71, 308, 886, 1304, 1403, 1802
RsaNI GTAC 6 cut(s) 70, 307, 885, 1303, 1402, 1801
RseI CAYNNNNRTG 2 cut(s) 354, 576
SapI GCTCTTC 1 cut(s) 267
SaqAI TTAA 9 cut(s) 435, 965, 1073, 1287, 1380, 1398, 1457, 1500, 1704
SatI GCNGC 5 cut(s) 776, 860, 987, 1080, 1844
Sau3AI GATC 7 cut(s) 4, 37, 94, 337, 1050, 1505, 1603
Sau96I GGNCC 3 cut(s) 109, 1178, 1756
ScaI AGTACT 1 cut(s) 1304
SchI GAGTC 1 cut(s) 1251
ScrFI CCNGG 1 cut(s) 1173
SduI GDGCHC 3 cut(s) 189, 1676, 1891
SexAI ACCWGGT 1 cut(s) 1171
SfaNI GCATC 2 cut(s) 1267, 1830
SfcI CTRYAG 1 cut(s) 1844
SfuI TTCGAA 1 cut(s) 1720
SinI GGWCC 3 cut(s) 109, 1178, 1756
SmiMI CAYNNNNRTG 2 cut(s) 354, 576
SmlI CTYRAG 1 cut(s) 1162
SmoI CTYRAG 1 cut(s) 1162
SsiI CCGC 5 cut(s) 491, 518, 656, 823, 928
SspMI CTAG 5 cut(s) 141, 471, 834, 1218, 1896
StyD4I CCNGG 1 cut(s) 1171
TaaI ACNGT 7 cut(s) 182, 332, 1420, 1433, 1447, 1551, 1760
TaiI ACGT 2 cut(s) 13, 1645
TaqI TCGA 2 cut(s) 1228, 1720
TatI WGTACW 2 cut(s) 884, 1302
TfiI GAWTC 4 cut(s) 227, 992, 1471, 1717
Tru1I TTAA 9 cut(s) 435, 965, 1073, 1287, 1380, 1398, 1457, 1500, 1704
Tru9I TTAA 9 cut(s) 435, 965, 1073, 1287, 1380, 1398, 1457, 1500, 1704
TscAI CASTG 5 cut(s) 187, 277, 1554, 1715, 1841
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 5 cut(s) 775, 859, 986, 1079, 1843
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 8 cut(s) 432, 1024, 1052, 1091, 1142, 1283, 1784, 1839
TspRI CASTG 5 cut(s) 187, 277, 1554, 1715, 1841
Van91I CCANNNNNTGG 1 cut(s) 1323
VpaK11BI GGWCC 3 cut(s) 109, 1178, 1756
XapI RAATTY 2 cut(s) 20, 1453
XmnI GAANNNNTTC 1 cut(s) 1098
XspI CTAG 5 cut(s) 141, 471, 834, 1218, 1896
ZrmI AGTACT 1 cut(s) 1304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.