Rh5CG467600

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000624
Physical Location & Seq
Forward (+)
65173336 .. 65175963
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG467600.1

Sequence Viewer

Length: 1776 bp
ATGGAATCAAAACCCTCTATGTTCCATGACCCTTTGGCTCTTCAAGCCACTGTGGAGTCTCTTCAAGAGGTTATTGGAGGAGGGAAGTGCCCAACTTCCCAAGACCTAAACCCTCTCTGTGTTGATCAAGATGTGTATGGAAATGGAAATGGGATGGTGGGTTCTTATAGGAGTGAGCTAATGGTTCCTGATGTGCTTAGTAAGAATGAATTGTTATTGCAATTTAACCTAGGGGCAGAGGAGGGTAGTAAGTTTCTTCCTAGATGTCAATTGATTAGCGCGGAGGGCAACAAGCCGTATGCTGTGGCGGATAGAAAGGCTGGAGATGTGGTAGTTACGACCCAGAAGAATGGGAGTGAGCATTTTCGTGTTGGTTCTAGAGGAAAGAAAAATCATCAAAGGGAAGATATAGATTTGGAGGATGGGAGGAGTAACAAGCAATCGGCGGTTTATATGGATGGTGAGGAGGGTGAGCTGTCTGATATTTTTGACAAGGTGTTGCTCTCCAAGCCTATCAAGAGTAAAGACGATCAAGTCAGTCAGAATGGAGCAAACAATAGCCTGCAGCAGGATGGGCTATCTGTTGGAATTGGTAATGGGAAGGTTTGTGGCAAGAATCAAGGCAACAAGAAGGAAGTGATAGATTTGAGGGTTCTTCTGATTTCATGTGCAGAAGCTGTATCTGTTGGTGACCGCGAGACTGCTAGTGAGCTACTAAAGCAGATTATGCAGCACTCTTCCCCATTTGGTGACTGTACTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTGAAGCGCGGTTGGCTGGCACAGGAACTCAAATATATACTGCTTTAACTACTAAACGAATGTCAGCTGCTGATTTGCTGAAGTTTTACCAAGCTTATATTGCAGTCTGCCCGTTCATGAAGTTGTTGATGATCTTTGCAAACGATATGATTTTAAAAGCTGCTGAGAAAGCAGAAACACTACATATAATAGATTTTGGTATCCTTTATGGTTTTCAATGGCCTGCTCTCATTCATTGCCTCTCAAGAAGACCTGGTGGACCTCCGAAATTACGCATTACAGGGGTAGAGTTTCCCTCAAGTGGTTTTCGACCAGAAGAAGGAGTCCAAGAGACAGGGCATCGCTTGGCAAAGTATTGTAGACGATTTAATGTTCATTTTGAGTACCATGGCATAGCCAAAAAATGGGAAACTCTCCAAGTTGATGACCTTAAAGTTCAAAGAAATGAGGTGCTTGCTGTTAATTGTCTCAACCGATTTGAGTACCTCCTTGATGAGACTGTTGTGGTAAACAGTCCAAGGGATACTGTTCTAAATTTAATTAGGAATTTGAATCCTGATGTCTTTGTCCAAAGTGTTGTTAATGGATCCCGTCATGCACCCTTCTTTGTTCCACGCTTCCAAGAGGCACTATTCCACTTCTCTGCATTGTTTGATATGTTGGATACCAATTTGCCTCGTGAAGATCAGATGAGATTGGGGTTTGAGAAAGAGTTTATTGGACGTGAAGTTTTGAATATAGTAGCTTGTGAGGGCTTTCAAAGAATTGTTATGCCAGAAACATACAAGCAGTGGCAGATTCGAAACATGAGGACTGGGTTCAGACAGCTGCCATTGGACCGTGAAGTCATGAACAAAATAAAGGATAAGGTAAAAATGGGGTACCACCCTTCTTTTGTTGTTGATGAAGATGGCCGCTGGATGCTGCATGGATGGAGGGGCAGAATTATGTATGCTTCTGCCTGTTGGGCACCATCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

591

Amino Acids

66.5

Weight (kDa)

6.92

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 217 - 586 3.1e-116 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 533, 1634
Acc65I GGTACC 1 cut(s) 1671
AccB1I GGYRCC 2 cut(s) 1671, 1759
AccB7I CCANNNNNTGG 1 cut(s) 1194
AccI GTMKAC 1 cut(s) 1150
AccII CGCG 3 cut(s) 281, 696, 799
AciI CCGC 6 cut(s) 281, 308, 446, 694, 799, 1705
AclWI GGATC 2 cut(s) 1371, 1384
AcoI YGGCCR 1 cut(s) 1702
AcsI RAATTY 2 cut(s) 1324, 1336
AcuI CTGAAG 1 cut(s) 890
AfaI GTAC 4 cut(s) 757, 1175, 1274, 1673
AfiI CCNNNNNNNGG 4 cut(s) 568, 1091, 1109, 1194
AgsI TTSAA 8 cut(s) 44, 65, 794, 1007, 1229, 1342, 1525, 1550
AjiI CACGTC 1 cut(s) 1514
AjnI CCWGG 1 cut(s) 1042
AloI GAACNNNNNNTCC 2 cut(s) 145, 177
AluBI AGCT 9 cut(s) 178, 475, 677, 712, 857, 884, 950, 1535, 1618
AluI AGCT 9 cut(s) 178, 475, 677, 712, 857, 884, 950, 1535, 1618
Alw26I GTCTC 5 cut(s) 63, 692, 1115, 1262, 1280
AlwI GGATC 2 cut(s) 1371, 1384
AlwNI CAGNNNCTG 2 cut(s) 677, 860
AoxI GGCC 2 cut(s) 1010, 1702
ApeKI GCWGC 6 cut(s) 565, 730, 857, 950, 1618, 1714
ApoI RAATTY 2 cut(s) 1324, 1336
Asp718I GGTACC 1 cut(s) 1671
AspA2I CCTAGG 1 cut(s) 229
AspLEI GCGC 2 cut(s) 281, 799
AspS9I GGNCC 2 cut(s) 1049, 1627
AsuHPI GGTGA 4 cut(s) 473, 482, 701, 761
AsuII TTCGAA 1 cut(s) 1591
AvaII GGWCC 2 cut(s) 1049, 1627
AvrII CCTAGG 1 cut(s) 229
BaeGI GKGCMC 2 cut(s) 92, 1762
BamHI GGATCC 1 cut(s) 1376
BanI GGYRCC 2 cut(s) 1671, 1759
BauI CACGAG 1 cut(s) 1467
BbsI GAAGAC 1 cut(s) 1045
BbvI GCAGC 6 cut(s) 577, 742, 844, 937, 1605, 1701
BccI CCATC 7 cut(s) 148, 416, 452, 566, 1694, 1716, 1771
BceAI ACGGC 1 cut(s) 280
BciT130I CCWGG 1 cut(s) 1044
BciVI GTATCC 3 cut(s) 1001, 1306, 1447
BclI TGATCA 1 cut(s) 124
BcoDI GTCTC 5 cut(s) 63, 692, 1115, 1262, 1280
BfaI CTAG 5 cut(s) 230, 261, 378, 705, 1767
BfmI CTRYAG 1 cut(s) 563
BfuI GTATCC 3 cut(s) 1001, 1306, 1447
BglI GCCNNNNNGGC 1 cut(s) 1757
BisI GCNGC 7 cut(s) 566, 731, 858, 951, 1619, 1705, 1715
BlnI CCTAGG 1 cut(s) 229
BlsI GCNGC 7 cut(s) 567, 732, 859, 952, 1620, 1706, 1716
Bme1390I CCNGG 1 cut(s) 1044
Bme18I GGWCC 2 cut(s) 1049, 1627
BmgBI CACGTC 1 cut(s) 1514
BmgT120I GGNCC 2 cut(s) 1049, 1627
BmiI GGNNCC 4 cut(s) 186, 1378, 1673, 1761
BmrFI CCNGG 1 cut(s) 1044
BmrI ACTGGG 1 cut(s) 1614
BmsI GCATC 2 cut(s) 1138, 1701
BmuI ACTGGG 1 cut(s) 1614
BpiI GAAGAC 1 cut(s) 1045
BplI GAGNNNNNCTC 4 cut(s) 754, 786, 1070, 1102
BpmI CTGGAG 1 cut(s) 342
Bpu14I TTCGAA 1 cut(s) 1591
BpuEI CTTGAG 2 cut(s) 1018, 1072
BsaJI CCNNGG 3 cut(s) 229, 1177, 1307
BsaXI ACNNNNNCTCC 2 cut(s) 315, 345
Bsc4I CCNNNNNNNGG 4 cut(s) 568, 1091, 1109, 1194
Bse1I ACTGG 1 cut(s) 1609
Bse3DI GCAATG 2 cut(s) 772, 1024
BseBI CCWGG 1 cut(s) 1044
BseDI CCNNGG 3 cut(s) 229, 1177, 1307
BseGI GGATG 6 cut(s) 159, 427, 463, 577, 1716, 1727
BseLI CCNNNNNNNGG 4 cut(s) 568, 1091, 1109, 1194
BseMI GCAATG 2 cut(s) 772, 1024
BseMII CTCAG 2 cut(s) 773, 945
BseNI ACTGG 1 cut(s) 1609
BseRI GAGGAG 4 cut(s) 93, 254, 442, 479
BseSI GKGCMC 2 cut(s) 92, 1762
BseXI GCAGC 6 cut(s) 577, 742, 844, 937, 1605, 1701
BsgI GTGCAG 1 cut(s) 690
Bsh1236I CGCG 3 cut(s) 281, 696, 799
BshFI GGCC 2 cut(s) 1012, 1704
BshNI GGYRCC 2 cut(s) 1671, 1759
BslI CCNNNNNNNGG 4 cut(s) 568, 1091, 1109, 1194
BsmAI GTCTC 5 cut(s) 63, 692, 1115, 1262, 1280
BsnI GGCC 2 cut(s) 1012, 1704
Bsp119I TTCGAA 1 cut(s) 1591
Bsp1286I GDGCHC 2 cut(s) 92, 1762
Bsp143I GATC 5 cut(s) 124, 529, 921, 1376, 1474
Bsp19I CCATGG 1 cut(s) 1177
BspACI CCGC 6 cut(s) 281, 308, 446, 694, 799, 1705
BspANI GGCC 2 cut(s) 1012, 1704
BspCNI CTCAG 2 cut(s) 772, 946
BspFNI CGCG 3 cut(s) 281, 696, 799
BspHI TCATGA 2 cut(s) 906, 1638
BspLI GGNNCC 4 cut(s) 186, 1378, 1673, 1761
BspMAI CTGCAG 1 cut(s) 567
BspPI GGATC 2 cut(s) 1371, 1384
BspQI GCTCTTC 1 cut(s) 45
BspT104I TTCGAA 1 cut(s) 1591
BspT107I GGYRCC 2 cut(s) 1671, 1759
BsrDI GCAATG 2 cut(s) 772, 1024
BsrI ACTGG 1 cut(s) 1609
BssECI CCNNGG 3 cut(s) 229, 1177, 1307
BssMI GATC 5 cut(s) 124, 529, 921, 1376, 1474
BssSI CACGAG 1 cut(s) 1467
BssT1I CCWWGG 3 cut(s) 229, 1177, 1307
Bst2BI CACGAG 1 cut(s) 1467
Bst2UI CCWGG 1 cut(s) 1044
Bst4CI ACNGT 6 cut(s) 52, 755, 1291, 1304, 1318, 1631
Bst6I CTCTTC 3 cut(s) 45, 66, 742
BstAPI GCANNNNNTGC 1 cut(s) 727
BstBI TTCGAA 1 cut(s) 1591
BstC8I GCNNGC 4 cut(s) 563, 808, 1014, 1245
BstDEI CTNAG 3 cut(s) 197, 759, 954
BstDSI CCRYGG 1 cut(s) 1177
BstEII GGTNACC 1 cut(s) 689
BstENI CCTNNNNNAGG 1 cut(s) 566
BstF5I GGATG 6 cut(s) 159, 427, 463, 577, 1716, 1727
BstFNI CGCG 3 cut(s) 281, 696, 799
BstHHI GCGC 2 cut(s) 281, 799
BstKTI GATC 5 cut(s) 127, 532, 924, 1379, 1477
BstMAI GTCTC 5 cut(s) 63, 692, 1115, 1262, 1280
BstMBI GATC 5 cut(s) 124, 529, 921, 1376, 1474
BstNI CCWGG 1 cut(s) 1044
BstPI GGTNACC 1 cut(s) 689
BstSCI CCNGG 1 cut(s) 1042
BstSFI CTRYAG 1 cut(s) 563
BstSLI GKGCMC 2 cut(s) 92, 1762
BstUI CGCG 3 cut(s) 281, 696, 799
BstV1I GCAGC 6 cut(s) 577, 742, 844, 937, 1605, 1701
BstV2I GAAGAC 1 cut(s) 1045
BstX2I RGATCY 1 cut(s) 1376
BstXI CCANNNNNNTGG 1 cut(s) 350
BstYI RGATCY 1 cut(s) 1376
BsuI GTATCC 3 cut(s) 1001, 1306, 1447
BsuRI GGCC 2 cut(s) 1012, 1704
BtgI CCRYGG 1 cut(s) 1177
BtgZI GCGATG 1 cut(s) 1115
BtrI CACGTC 1 cut(s) 1514
BtsCI GGATG 6 cut(s) 159, 427, 463, 577, 1716, 1727
BtsI GCAGTG 1 cut(s) 1586
BtsIMutI CAGTG 2 cut(s) 48, 1586
Cac8I GCNNGC 4 cut(s) 563, 808, 1014, 1245
CaiI CAGNNNCTG 2 cut(s) 677, 860
CciI TCATGA 2 cut(s) 906, 1638
CfoI GCGC 2 cut(s) 281, 799
Cfr13I GGNCC 2 cut(s) 1049, 1627
CsiI ACCWGGT 1 cut(s) 1042
Csp6I GTAC 4 cut(s) 756, 1174, 1273, 1672
CviAII CATG 8 cut(s) 26, 666, 907, 1178, 1385, 1597, 1639, 1718
CviQI GTAC 4 cut(s) 756, 1174, 1273, 1672
DdeI CTNAG 3 cut(s) 197, 759, 954
DpnI GATC 5 cut(s) 126, 531, 923, 1378, 1476
DpnII GATC 5 cut(s) 124, 529, 921, 1376, 1474
DraI TTTAAA 1 cut(s) 945
DrdI GACNNNNNNGTC 2 cut(s) 533, 1634
DseDI GACNNNNNNGTC 2 cut(s) 533, 1634
EaeI YGGCCR 1 cut(s) 1702
Eam1104I CTCTTC 3 cut(s) 45, 66, 742
EarI CTCTTC 3 cut(s) 45, 66, 742
EciI GGCGGA 1 cut(s) 323
Eco130I CCWWGG 3 cut(s) 229, 1177, 1307
Eco47I GGWCC 2 cut(s) 1049, 1627
Eco57I CTGAAG 1 cut(s) 890
Eco91I GGTNACC 1 cut(s) 689
EcoNI CCTNNNNNAGG 1 cut(s) 566
EcoO65I GGTNACC 1 cut(s) 689
EcoRII CCWGG 1 cut(s) 1042
EcoT14I CCWWGG 3 cut(s) 229, 1177, 1307
ErhI CCWWGG 3 cut(s) 229, 1177, 1307
FaeI CATG 8 cut(s) 29, 669, 910, 1181, 1388, 1600, 1642, 1721
FatI CATG 8 cut(s) 25, 665, 906, 1177, 1384, 1596, 1638, 1717
FbaI TGATCA 1 cut(s) 124
FblI GTMKAC 1 cut(s) 1150
Fnu4HI GCNGC 7 cut(s) 566, 731, 858, 951, 1619, 1705, 1715
FokI GGATG 6 cut(s) 166, 434, 470, 584, 1723, 1734
Fsp4HI GCNGC 7 cut(s) 566, 731, 858, 951, 1619, 1705, 1715
FspBI CTAG 5 cut(s) 230, 261, 378, 705, 1767
GlaI GCGC 2 cut(s) 280, 798
GluI GCNGC 7 cut(s) 566, 731, 858, 951, 1619, 1705, 1715
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 2 cut(s) 1012, 1704
HhaI GCGC 2 cut(s) 281, 799
Hin1II CATG 8 cut(s) 29, 669, 910, 1181, 1388, 1600, 1642, 1721
Hin6I GCGC 2 cut(s) 279, 797
HinP1I GCGC 2 cut(s) 279, 797
HindIII AAGCTT 1 cut(s) 882
HinfI GANTC 6 cut(s) 5, 56, 616, 1113, 1342, 1588
HphI GGTGA 4 cut(s) 473, 482, 701, 761
Hpy166II GTNNAC 3 cut(s) 1049, 1151, 1300
Hpy188I TCNGA 7 cut(s) 481, 543, 660, 762, 1056, 1479, 1613
Hpy8I GTNNAC 3 cut(s) 1049, 1151, 1300
HpyAV CCTTC 5 cut(s) 595, 625, 1103, 1402, 1689
HpyCH4III ACNGT 6 cut(s) 52, 755, 1291, 1304, 1318, 1631
HpyCH4IV ACGT 1 cut(s) 1513
HpyF3I CTNAG 3 cut(s) 197, 759, 954
HpySE526I ACGT 1 cut(s) 1513
Hsp92II CATG 8 cut(s) 29, 669, 910, 1181, 1388, 1600, 1642, 1721
HspAI GCGC 2 cut(s) 279, 797
KpnI GGTACC 1 cut(s) 1675
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 5 cut(s) 124, 529, 921, 1376, 1474
LguI GCTCTTC 1 cut(s) 45
LmnI GCTCC 1 cut(s) 548
Lsp1109I GCAGC 6 cut(s) 577, 742, 844, 937, 1605, 1701
LweI GCATC 2 cut(s) 1138, 1701
MabI ACCWGGT 1 cut(s) 1042
MaeI CTAG 5 cut(s) 230, 261, 378, 705, 1767
MaeII ACGT 1 cut(s) 1513
MaeIII GTNAC 4 cut(s) 334, 431, 689, 749
MalI GATC 5 cut(s) 126, 531, 923, 1378, 1476
MboI GATC 5 cut(s) 124, 529, 921, 1376, 1474
MfeI CAATTG 1 cut(s) 269
MflI RGATCY 1 cut(s) 1376
MhlI GDGCHC 2 cut(s) 92, 1762
MlyI GAGTC 2 cut(s) 65, 1122
MmeI TCCRAC 2 cut(s) 565, 1431
MseI TTAA 8 cut(s) 225, 836, 944, 1158, 1221, 1251, 1328, 1371
MslI CAYNNNNRTG 1 cut(s) 366
MspA1I CMGCKG 3 cut(s) 857, 1618, 1707
MspR9I CCNGG 1 cut(s) 1044
MunI CAATTG 1 cut(s) 269
MvaI CCWGG 1 cut(s) 1044
MvnI CGCG 3 cut(s) 281, 696, 799
NcoI CCATGG 1 cut(s) 1177
NdeII GATC 5 cut(s) 124, 529, 921, 1376, 1474
NlaIII CATG 8 cut(s) 29, 669, 910, 1181, 1388, 1600, 1642, 1721
NlaIV GGNNCC 4 cut(s) 186, 1378, 1673, 1761
NmuCI GTSAC 2 cut(s) 689, 749
NspV TTCGAA 1 cut(s) 1591
PagI TCATGA 2 cut(s) 906, 1638
PciSI GCTCTTC 1 cut(s) 45
PfeI GAWTC 4 cut(s) 5, 616, 1342, 1588
PflMI CCANNNNNTGG 1 cut(s) 1194
PkrI GCNGC 7 cut(s) 567, 732, 859, 952, 1620, 1706, 1716
PleI GAGTC 2 cut(s) 64, 1121
PpsI GAGTC 2 cut(s) 64, 1121
Psp6I CCWGG 1 cut(s) 1042
PspEI GGTNACC 1 cut(s) 689
PspGI CCWGG 1 cut(s) 1042
PspN4I GGNNCC 4 cut(s) 186, 1378, 1673, 1761
PspPI GGNCC 2 cut(s) 1049, 1627
PstI CTGCAG 1 cut(s) 567
PstNI CAGNNNCTG 2 cut(s) 677, 860
PsuI RGATCY 1 cut(s) 1376
PvuII CAGCTG 2 cut(s) 857, 1618
RsaI GTAC 4 cut(s) 757, 1175, 1274, 1673
RsaNI GTAC 4 cut(s) 756, 1174, 1273, 1672
RseI CAYNNNNRTG 1 cut(s) 366
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 8 cut(s) 225, 836, 944, 1158, 1221, 1251, 1328, 1371
SatI GCNGC 7 cut(s) 566, 731, 858, 951, 1619, 1705, 1715
Sau3AI GATC 5 cut(s) 124, 529, 921, 1376, 1474
Sau96I GGNCC 2 cut(s) 1049, 1627
SchI GAGTC 2 cut(s) 65, 1122
ScrFI CCNGG 1 cut(s) 1044
SduI GDGCHC 2 cut(s) 92, 1762
SexAI ACCWGGT 1 cut(s) 1042
SfaNI GCATC 2 cut(s) 1138, 1701
SfcI CTRYAG 1 cut(s) 563
SfuI TTCGAA 1 cut(s) 1591
SinI GGWCC 2 cut(s) 1049, 1627
SmiMI CAYNNNNRTG 1 cut(s) 366
SmlI CTYRAG 2 cut(s) 1033, 1087
SmoI CTYRAG 2 cut(s) 1033, 1087
SsiI CCGC 6 cut(s) 281, 308, 446, 694, 799, 1705
SspMI CTAG 5 cut(s) 230, 261, 378, 705, 1767
StyD4I CCNGG 1 cut(s) 1042
StyI CCWWGG 3 cut(s) 229, 1177, 1307
TaaI ACNGT 6 cut(s) 52, 755, 1291, 1304, 1318, 1631
TaiI ACGT 1 cut(s) 1516
TaqI TCGA 2 cut(s) 1099, 1591
TatI WGTACW 1 cut(s) 755
TauI GCSGC 1 cut(s) 1707
TfiI GAWTC 4 cut(s) 5, 616, 1342, 1588
Tru1I TTAA 8 cut(s) 225, 836, 944, 1158, 1221, 1251, 1328, 1371
Tru9I TTAA 8 cut(s) 225, 836, 944, 1158, 1221, 1251, 1328, 1371
TscAI CASTG 2 cut(s) 55, 1586
TseFI GTSAC 2 cut(s) 689, 749
TseI GCWGC 6 cut(s) 565, 730, 857, 950, 1618, 1714
Tsp45I GTSAC 2 cut(s) 689, 749
TspDTI ATGAA 8 cut(s) 222, 654, 895, 923, 1013, 1154, 1655, 1710
TspRI CASTG 2 cut(s) 55, 1586
Van91I CCANNNNNTGG 1 cut(s) 1194
VpaK11BI GGWCC 2 cut(s) 1049, 1627
XagI CCTNNNNNAGG 1 cut(s) 566
XapI RAATTY 2 cut(s) 1324, 1336
XbaI TCTAGA 1 cut(s) 377
XmaJI CCTAGG 1 cut(s) 229
XmiI GTMKAC 1 cut(s) 1150
XspI CTAG 5 cut(s) 230, 261, 378, 705, 1767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.