Rorug05G0372200

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000624
Physical Location & Seq
Forward (+)
50186277 .. 50188332
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0372200.1

Sequence Viewer

Length: 1644 bp
ATGAAATATTTAAACGTCCGCAAGTTGGTTAAATCAGTCCACAGAATCCCCACGGATTCTATGTTGTTTCAGCATGATAGGGACACCCATTTGAAGATTATTGAGATTCTGGGCCGAGCTTCGAAGCTCAACCATGGCCGGTGTATCCTACTGGATATGCCCAAGAAGGGTGTGCAATGGGATGAGGACCTCTTCATTCATCTTATTGATAGTTATGGAAAGGCCGGCATTGTTCAGGAGTCTGTTAAGCTTTTCAATCAGATGAAGGAATTGGGTGTGGAGCGAAGTCTCAAGTCGTATGAGGCTCTGTTTAAGAGCCTTTTGCGGAGGGGGCGGTATATGATGGGGAAGAGGTACTTTAATCACATGTTGGCTGAGGGAATAGAGCCCACTAGGCATACTTATAACATTATGATTTGGGGTTTCTTCTTGTCCTTGAGGTTGGAGACTGCAAAACGGTTTTTCGAGGACATGAAAAGTAGAGGAATTAATCCTGATGTTGTCACCTATAACACGATGATTAATGGGTACAATCGGTTTAAGCTGATGGATGAGGCCGAGCAGTTGTTTGTAGAGTTGAAGGGAAAGAATATACAGCCCAATGTTATAAGTTATACTACCATGATTAAAGGGTATGTTTCTGTTGGGAGAGTGGATGATGGATTCAGGCTGTTTCAAGAGATGAAGTCTTTTGGTATTAAGCCTAATGATGTCACGTTTTCGACTTTGTTACCAGGGCTTTGCGATGCGGAGAAAAAGGATGAAGCTCAGAATATATTGAATGAGATGGTTGAGAGGCATATTGCTCCCAAGGATAATTCAATCTTCGAGAAATTGTTATATTGTCAGTGCAAGTCTGGGGATTTGGATGCGGCTGCCGATGTGCTCAAGGCAATGATTAGGCTGCACATTCCGACAGAGGCTGGTCATTACAGTATCCTTATTGAGAACTTCTGCAAGGCTGGGGTGTATGATCAAGCAGTTCACTTGCTTGATAAGCTCATTGAGAAAGAAATTATTATGAGGCCCCAGAGTTCTATGGAATTGGAGGCTAGTGCTTACAACCCAATGATTGAGTATCTGTGCAACCATGGGCAGACAGGTAAAGCTGAAGTCCTTTTCAGGCAGTTGATGAAAAAGGGAGTTCAGGATTCAGTTGCCTTCAATAATTTGATCCGTGGGCATGCTAAGGAAGGAAATTGTGATTCTGCTTTTGAGATTTTAAAGATTATGGGTAGAAGAGGGGTTCCTAGAGAAGCAGATTCCTACAAGTTGCTCATTAAGAGCTACCTAAGTAAAGGTGAACCAGCTGATGCTAAAACAGCTTTGGACAGTATGATTGAAAATGGGCATGTTCCAGAATCATCACTATTCAGGTCAGTAATGGAGAGTCTATTTGAAGATGGTAGAGTTCAAACAGCAAGCCGCGTAATGAAGAGTATGGTAGAGAAGGGAGTGAATGAGAACATGGATTTAGTCGCCAAGGTTTTGGAAGCCCTGTTTATCAGAGGTCATGTTGAGGAAGCACTTGGTCGGGTTGATCTACTTATGCAAAGTGGGTGTGCACCTGAGTTTGATAGCCTCTTATCTCTGCTTGCTGAAAAAGGAAAGACCATTGCTGATAAGTCGACGTTCAATCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

547

Amino Acids

62.21

Weight (kDa)

7.97

Isoelectric Point (pI)

33.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 67 - 108 8.5e-06 PPR repeat family
PPR_2 PF13041 99 - 142 2e-07 PPR repeat family
PPR_2 PF13041 136 - 177 3.1e-12 PPR repeat family
PPR_3 PF13812 154 - 213 1.9e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 161 - 192 3.2e-10 PPR repeat
PPR_2 PF13041 165 - 213 2.6e-16 PPR repeat family
PPR PF01535 168 - 198 1.5e-07 PPR repeat
PPR_1 PF12854 196 - 229 2.3e-09 PPR repeat
PPR_2 PF13041 200 - 249 6.8e-18 PPR repeat family
PPR PF01535 203 - 233 3.2e-08 PPR repeat
PPR_long PF17177 218 - 337 1.2e-12 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 223 - 270 1.6e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 232 - 264 6e-08 PPR repeat
PPR_2 PF13041 384 - 428 4.9e-07 PPR repeat family
TPR_24 PF23276 395 - 511 3.4e-08 Fungal tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 405, 608
AccI GTMKAC 1 cut(s) 1628
AccII CGCG 1 cut(s) 1428
AciI CCGC 6 cut(s) 19, 325, 334, 749, 872, 1426
AclWI GGATC 1 cut(s) 1168
AcoI YGGCCR 1 cut(s) 136
AcuI CTGAAG 1 cut(s) 1131
AfaI GTAC 2 cut(s) 356, 530
AfiI CCNNNNNNNGG 2 cut(s) 25, 167
AflIII ACRYGT 1 cut(s) 366
AjnI CCWGG 1 cut(s) 733
Alw21I GWGCWC 2 cut(s) 888, 1567
Alw26I GTCTC 2 cut(s) 293, 440
Alw44I GTGCAC 1 cut(s) 1563
AlwI GGATC 1 cut(s) 1168
AlwNI CAGNNNCTG 1 cut(s) 923
AoxI GGCC 5 cut(s) 112, 136, 222, 555, 1025
ApaLI GTGCAC 1 cut(s) 1563
ApeKI GCWGC 2 cut(s) 875, 904
AseI ATTAAT 2 cut(s) 489, 522
AspS9I GGNCC 3 cut(s) 112, 187, 1026
AsuHPI GGTGA 2 cut(s) 496, 1313
AsuII TTCGAA 1 cut(s) 122
AvaII GGWCC 1 cut(s) 187
BaeGI GKGCMC 1 cut(s) 1567
BanII GRGCYC 1 cut(s) 390
Bbv12I GWGCWC 2 cut(s) 888, 1567
BbvCI CCTCAGC 1 cut(s) 375
BbvI GCAGC 2 cut(s) 862, 891
BccI CCATC 5 cut(s) 337, 541, 653, 781, 1397
BciT130I CCWGG 1 cut(s) 735
BciVI GTATCC 2 cut(s) 155, 947
BclI TGATCA 1 cut(s) 973
BcoDI GTCTC 2 cut(s) 293, 440
BfaI CTAG 3 cut(s) 393, 1053, 1251
BfuI GTATCC 2 cut(s) 155, 947
BglI GCCNNNNNGGC 1 cut(s) 394
BisI GCNGC 4 cut(s) 873, 876, 905, 1426
BlsI GCNGC 4 cut(s) 874, 877, 906, 1427
Bme1390I CCNGG 1 cut(s) 735
Bme18I GGWCC 1 cut(s) 187
BmgT120I GGNCC 3 cut(s) 112, 187, 1026
BmiI GGNNCC 2 cut(s) 1028, 1248
BmrFI CCNGG 1 cut(s) 735
BmsI GCATC 3 cut(s) 736, 859, 1303
Bpu10I CCTNAGC 2 cut(s) 375, 1188
Bpu14I TTCGAA 1 cut(s) 122
BpuEI CTTGAG 3 cut(s) 275, 457, 872
BsaJI CCNNGG 7 cut(s) 51, 133, 734, 810, 1090, 1177, 1482
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 167
Bse118I RCCGGY 2 cut(s) 138, 224
Bse1I ACTGG 1 cut(s) 156
Bse3DI GCAATG 3 cut(s) 182, 900, 1614
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 7 cut(s) 51, 133, 734, 810, 1090, 1177, 1482
BseGI GGATG 5 cut(s) 187, 556, 661, 766, 874
BseLI CCNNNNNNNGG 2 cut(s) 25, 167
BseMI GCAATG 3 cut(s) 182, 900, 1614
BseMII CTCAG 3 cut(s) 366, 782, 1560
BseNI ACTGG 1 cut(s) 156
BseSI GKGCMC 1 cut(s) 1567
BseXI GCAGC 2 cut(s) 862, 891
BseYI CCCAGC 1 cut(s) 962
BsgI GTGCAG 1 cut(s) 890
Bsh1236I CGCG 1 cut(s) 1428
BshFI GGCC 5 cut(s) 114, 138, 224, 557, 1027
BsiHKAI GWGCWC 2 cut(s) 888, 1567
BsiSI CCGG 2 cut(s) 139, 225
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 25, 167
BsmAI GTCTC 2 cut(s) 293, 440
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 5 cut(s) 114, 138, 224, 557, 1027
Bsp119I TTCGAA 1 cut(s) 122
Bsp1286I GDGCHC 3 cut(s) 390, 888, 1567
Bsp143I GATC 3 cut(s) 973, 1173, 1540
Bsp19I CCATGG 2 cut(s) 133, 1090
BspACI CCGC 6 cut(s) 19, 325, 334, 749, 872, 1426
BspANI GGCC 5 cut(s) 114, 138, 224, 557, 1027
BspCNI CTCAG 3 cut(s) 367, 781, 1561
BspFNI CGCG 1 cut(s) 1428
BspLI GGNNCC 2 cut(s) 1028, 1248
BspPI GGATC 1 cut(s) 1168
BspT104I TTCGAA 1 cut(s) 122
BsrDI GCAATG 3 cut(s) 182, 900, 1614
BsrFI RCCGGY 2 cut(s) 138, 224
BsrI ACTGG 1 cut(s) 156
BssAI RCCGGY 2 cut(s) 138, 224
BssECI CCNNGG 7 cut(s) 51, 133, 734, 810, 1090, 1177, 1482
BssMI GATC 3 cut(s) 973, 1173, 1540
BssT1I CCWWGG 4 cut(s) 133, 810, 1090, 1482
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 3 cut(s) 459, 935, 1334
Bst6I CTCTTC 4 cut(s) 197, 344, 1234, 1430
BstBI TTCGAA 1 cut(s) 122
BstC8I GCNNGC 4 cut(s) 226, 1185, 1423, 1596
BstDEI CTNAG 5 cut(s) 375, 768, 1188, 1292, 1569
BstDSI CCRYGG 4 cut(s) 51, 133, 1090, 1177
BstF5I GGATG 5 cut(s) 187, 556, 661, 766, 874
BstFNI CGCG 1 cut(s) 1428
BstKTI GATC 3 cut(s) 976, 1176, 1543
BstMAI GTCTC 2 cut(s) 293, 440
BstMBI GATC 3 cut(s) 973, 1173, 1540
BstMWI GCNNNNNNNGC 4 cut(s) 331, 394, 997, 1322
BstNI CCWGG 1 cut(s) 735
BstNSI RCATGY 3 cut(s) 370, 1187, 1355
BstSCI CCNGG 1 cut(s) 733
BstSLI GKGCMC 1 cut(s) 1567
BstUI CGCG 1 cut(s) 1428
BstV1I GCAGC 2 cut(s) 862, 891
BstXI CCANNNNNNTGG 1 cut(s) 1489
BsuI GTATCC 2 cut(s) 155, 947
BsuRI GGCC 5 cut(s) 114, 138, 224, 557, 1027
BtgI CCRYGG 4 cut(s) 51, 133, 1090, 1177
BtgZI GCGATG 1 cut(s) 759
BtsCI GGATG 5 cut(s) 187, 556, 661, 766, 874
BtsIMutI CAGTG 1 cut(s) 854
Cac8I GCNNGC 4 cut(s) 226, 1185, 1423, 1596
CaiI CAGNNNCTG 1 cut(s) 923
Cfr10I RCCGGY 2 cut(s) 138, 224
Cfr13I GGNCC 3 cut(s) 112, 187, 1026
Csp6I GTAC 2 cut(s) 355, 529
CviQI GTAC 2 cut(s) 355, 529
DdeI CTNAG 5 cut(s) 375, 768, 1188, 1292, 1569
DpnI GATC 3 cut(s) 975, 1175, 1542
DpnII GATC 3 cut(s) 973, 1173, 1540
DraI TTTAAA 2 cut(s) 12, 1224
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 4 cut(s) 197, 344, 1234, 1430
EarI CTCTTC 4 cut(s) 197, 344, 1234, 1430
Eco130I CCWWGG 4 cut(s) 133, 810, 1090, 1482
Eco24I GRGCYC 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 1131
EcoO109I RGGNCCY 2 cut(s) 187, 1026
EcoRII CCWGG 1 cut(s) 733
EcoT14I CCWWGG 4 cut(s) 133, 810, 1090, 1482
EcoT38I GRGCYC 1 cut(s) 390
ErhI CCWWGG 4 cut(s) 133, 810, 1090, 1482
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 1 cut(s) 95
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 1 cut(s) 1628
Fnu4HI GCNGC 4 cut(s) 873, 876, 905, 1426
FokI GGATG 5 cut(s) 194, 563, 668, 773, 881
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 4 cut(s) 873, 876, 905, 1426
FspBI CTAG 3 cut(s) 393, 1053, 1251
GluI GCNGC 4 cut(s) 873, 876, 905, 1426
GsaI CCCAGC 1 cut(s) 966
HaeIII GGCC 5 cut(s) 114, 138, 224, 557, 1027
HapII CCGG 2 cut(s) 139, 225
HincII GTYRAC 1 cut(s) 1629
HindII GTYRAC 1 cut(s) 1629
HindIII AAGCTT 1 cut(s) 248
HpaII CCGG 2 cut(s) 139, 225
HphI GGTGA 2 cut(s) 496, 1313
Hpy166II GTNNAC 5 cut(s) 40, 985, 1304, 1565, 1629
Hpy188I TCNGA 4 cut(s) 261, 771, 915, 1508
Hpy188III TCNNGA 6 cut(s) 236, 494, 677, 829, 1148, 1358
Hpy8I GTNNAC 5 cut(s) 40, 985, 1304, 1565, 1629
Hpy99I CGWCG 1 cut(s) 1633
HpyAV CCTTC 6 cut(s) 160, 259, 574, 1171, 1187, 1444
HpyCH4III ACNGT 3 cut(s) 459, 935, 1334
HpyCH4IV ACGT 3 cut(s) 15, 716, 1631
HpyCH4V TGCA 8 cut(s) 175, 452, 852, 907, 957, 1086, 1552, 1565
HpyF10VI GCNNNNNNNGC 4 cut(s) 331, 394, 997, 1322
HpyF3I CTNAG 5 cut(s) 375, 768, 1188, 1292, 1569
HpySE526I ACGT 3 cut(s) 15, 716, 1631
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 3 cut(s) 973, 1173, 1540
LmnI GCTCC 2 cut(s) 280, 811
Lsp1109I GCAGC 2 cut(s) 862, 891
LweI GCATC 3 cut(s) 736, 859, 1303
MaeI CTAG 3 cut(s) 393, 1053, 1251
MaeII ACGT 3 cut(s) 15, 716, 1631
MaeIII GTNAC 3 cut(s) 502, 712, 729
MalI GATC 3 cut(s) 975, 1175, 1542
MboI GATC 3 cut(s) 973, 1173, 1540
MboII GAAGA 8 cut(s) 106, 184, 361, 418, 817, 1251, 1412, 1447
MhlI GDGCHC 3 cut(s) 390, 888, 1567
MluCI AATT 8 cut(s) 269, 486, 817, 833, 1014, 1043, 1168, 1198
MlyI GAGTC 2 cut(s) 248, 1399
MmeI TCCRAC 2 cut(s) 423, 938
MroNI GCCGGC 1 cut(s) 224
MspA1I CMGCKG 1 cut(s) 1310
MspI CCGG 2 cut(s) 139, 225
MspR9I CCNGG 1 cut(s) 735
MvaI CCWGG 1 cut(s) 735
MvnI CGCG 1 cut(s) 1428
MwoI GCNNNNNNNGC 4 cut(s) 331, 394, 997, 1322
NaeI GCCGGC 1 cut(s) 226
NcoI CCATGG 2 cut(s) 133, 1090
NdeII GATC 3 cut(s) 973, 1173, 1540
NgoMIV GCCGGC 1 cut(s) 224
NlaIV GGNNCC 2 cut(s) 1028, 1248
NmeAIII GCCGAG 2 cut(s) 140, 583
NmuCI GTSAC 2 cut(s) 502, 712
NspI RCATGY 3 cut(s) 370, 1187, 1355
NspV TTCGAA 1 cut(s) 122
PaeI GCATGC 1 cut(s) 1187
PciI ACATGT 1 cut(s) 366
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 8 cut(s) 45, 56, 106, 663, 1151, 1205, 1262, 1361
PkrI GCNGC 4 cut(s) 874, 877, 906, 1427
PleI GAGTC 2 cut(s) 247, 1398
PpsI GAGTC 2 cut(s) 247, 1398
PpuMI RGGWCCY 1 cut(s) 187
PscI ACATGT 1 cut(s) 366
PshBI ATTAAT 2 cut(s) 489, 522
PsiI TTATAA 2 cut(s) 405, 608
Psp5II RGGWCCY 1 cut(s) 187
Psp6I CCWGG 1 cut(s) 733
PspFI CCCAGC 1 cut(s) 962
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 2 cut(s) 1028, 1248
PspPI GGNCC 3 cut(s) 112, 187, 1026
PspPPI RGGWCCY 1 cut(s) 187
PstNI CAGNNNCTG 1 cut(s) 923
PvuII CAGCTG 1 cut(s) 1310
RsaI GTAC 2 cut(s) 356, 530
RsaNI GTAC 2 cut(s) 355, 529
SalI GTCGAC 1 cut(s) 1627
SatI GCNGC 4 cut(s) 873, 876, 905, 1426
Sau3AI GATC 3 cut(s) 973, 1173, 1540
Sau96I GGNCC 3 cut(s) 112, 187, 1026
SchI GAGTC 2 cut(s) 248, 1399
ScrFI CCNGG 1 cut(s) 735
SduI GDGCHC 3 cut(s) 390, 888, 1567
SfaNI GCATC 3 cut(s) 736, 859, 1303
SfuI TTCGAA 1 cut(s) 122
SinI GGWCC 1 cut(s) 187
SmlI CTYRAG 3 cut(s) 290, 436, 887
SmoI CTYRAG 3 cut(s) 290, 436, 887
SphI GCATGC 1 cut(s) 1187
Sse9I AATT 8 cut(s) 269, 486, 817, 833, 1014, 1043, 1168, 1198
SsiI CCGC 6 cut(s) 19, 325, 334, 749, 872, 1426
SspI AATATT 1 cut(s) 8
SspMI CTAG 3 cut(s) 393, 1053, 1251
StyD4I CCNGG 1 cut(s) 733
StyI CCWWGG 4 cut(s) 133, 810, 1090, 1482
TaaI ACNGT 3 cut(s) 459, 935, 1334
TaiI ACGT 3 cut(s) 18, 719, 1634
TaqI TCGA 5 cut(s) 122, 465, 722, 828, 1628
TasI AATT 8 cut(s) 269, 486, 817, 833, 1014, 1043, 1168, 1198
TauI GCSGC 2 cut(s) 875, 1428
TfiI GAWTC 8 cut(s) 45, 56, 106, 663, 1151, 1205, 1262, 1361
TscAI CASTG 1 cut(s) 854
TseFI GTSAC 2 cut(s) 502, 712
TseI GCWGC 2 cut(s) 875, 904
Tsp45I GTSAC 2 cut(s) 502, 712
TspDTI ATGAA 9 cut(s) 17, 184, 188, 278, 488, 698, 777, 1148, 1448
TspGWI ACGGA 2 cut(s) 68, 1166
TspRI CASTG 1 cut(s) 854
VneI GTGCAC 1 cut(s) 1563
VpaK11BI GGWCC 1 cut(s) 187
VspI ATTAAT 2 cut(s) 489, 522
XceI RCATGY 3 cut(s) 370, 1187, 1355
XmiI GTMKAC 1 cut(s) 1628
XspI CTAG 3 cut(s) 393, 1053, 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.