Rh5AG430000

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000624
Physical Location & Seq
Forward (+)
73956189 .. 73958832
2644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG430000.1

Sequence Viewer

Length: 1935 bp
ATGTTGAATGAGTACCCAAGTAGTGATCCTTCTCAAGATCTTAGCTTTGTGGACCCAAATCCCACTAAATCTTCATCAAGCTTTAGCCCTGAGAGAGATGATGGTGAGTTTTCTGACAGTGTGCTCAAGTACATAAACCAGGTGCTTATTAAGGAGGACATTGAATCAAAACCCTCTATGTTCCATGACCCTTTGGCTCTTCAAGCCACTGAGGAGTCTCTTCATGAGGTTATTGGAGGAGGGAAGTGCCGAACTTCTCAAGACCTAAACCCTCTTTGTGTTGATCAAGATGTGTATGGAAATGGGAATGGGATGGTGGGTTCTTATAGGAGTGAGCTAATGGTTCCTGATGTGCTTAGTAAGAATGAATTGTTATTGCAATTTAACGCAGGGGCAGAGGAGGGTAGTAAGTTTCTTCCTAGAGTTCAATTGATTAGCGCGGAGGGCAACAAGCCGTATGCTGTGGCGGATAGAAAGGCTGGAGATGTGGTAGTTACGACCCAGAAGAATGGGAGTGAGCATTTTCGTGTTGGTTCTAGACGAAAGAAAAATCATCAAAGGGAAGATATAGATTTGGAGGATGGGAGGAGTAACAAGCAATCGGCAGTTTATATGCATGGTGAGGAGGGTGAGCTGTCTGATATTTTTGACAAGGTGTTGCTCTCCAAGCCTATCAAGAGTAAAGACGATCAAGTCAGTCAGAATGGAGCAAACAATAGCCTGCAGCAGGATGGGCTATCTGTTGGAATTGGTAATGGGAAGGTTTGCGGCAAGAATCAAGGCAACAAGAAGGAAGTGATAGATTTGAGGGCTCTTCTGATTTCATGTGCAGAAGCTGTATCTGTTGATGACCGCGAGACTGCTAGTGAGCTACTAAAGCAGATTATGCAGCACTCTTCCCCATTTGGTGATTGTACTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTGAAGCGCGGTTGGCTGGCACAGGAACTCAAATATATACTGCTTTAACTACTAACCGAATGTCAGCTGCTGATTCGCTGAAGTTTTACCAAGCTTATATTGCAGTCTGCCCGTTCATGAAGTTGTTGATGATCTTTGCAAACGATATGATTTTAAAAGCTGCTGGGAAAGCAGAAACACTACATATAATAGATTTTGGTATCCGTTATGGTTTTCAATGGCCTGCTCTCATTCATTGCCTCTCAAGAAGACCTGGTGGACCTCCCAAATTACGCATTACAGGGTTAGAGTTTCCCTCAAGTGGTTTTCGACCAGAAGAAGGAGTCCAAGAGACAGGGCATCGCTTGGCAAAGTATTGTAGGCGATTTAATGTTCATTTTGAGTACCATGGCATAGCCAAAAAATGGGAAACTCTCCAAGTTGATGACCTTAGAGTTCAAAGAAATGAGGTACTTGCTGTTAATTGTCTCAACCGATTCAAGAACCTCCTTGATGAGACTGTTGTGGTAAACAGTCCAAGGGATACTGTTCTAAATTTAATTAGGAATTTGAATCCTGATGTCTTTGTCCAAAGTGTTGTTAATGGATGCCATCATGCACCCTTCTTTGTTCCACGCTTCGAAGAGGCACTATTCCACTTCTCTGCATTGTTTGATATGTTGGATACCAATTTGCCTCGTGAAGATCAGATGAGATTGGGGTTTGAGAAAGAGTTTATTGGACGTGAAGTTGTGAATATAGTAGCTTGTGAGGGCTTTCAAAGAATTGTTAGGCCAGAAACATACAAGCAGTGGCAGATTCGAAACATGAGGGCTGGGTTCAGACAGCTGCCATTGGACCGTGAAGTCATGAACAAAATAAAGGATAAGGTAAAAATGGGGTACCACCCTTCTTTTGTGGTAGATGAAGATGGCCGCTGGATGCTGCATGAATGGAAGGGCAGAATTATGTATGCTTCTGCCTGTTGGGCACCATCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

644

Amino Acids

72.57

Weight (kDa)

6.31

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 269 - 639 6e-117 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 692, 1793
Acc65I GGTACC 1 cut(s) 1830
AccB1I GGYRCC 2 cut(s) 1830, 1918
AccB7I CCANNNNNTGG 1 cut(s) 1353
AccII CGCG 3 cut(s) 440, 855, 958
AciI CCGC 6 cut(s) 440, 467, 768, 853, 958, 1864
AclWI GGATC 1 cut(s) 20
AcoI YGGCCR 1 cut(s) 1861
AcsI RAATTY 2 cut(s) 1483, 1495
AcuI CTGAAG 1 cut(s) 1049
AfaI GTAC 6 cut(s) 14, 131, 916, 1334, 1401, 1832
AfiI CCNNNNNNNGG 4 cut(s) 727, 1250, 1268, 1353
AjiI CACGTC 1 cut(s) 1673
AjnI CCWGG 2 cut(s) 138, 1201
AloI GAACNNNNNNTCC 2 cut(s) 304, 336
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 5 cut(s) 222, 851, 1274, 1421, 1439
AlwI GGATC 1 cut(s) 20
AlwNI CAGNNNCTG 2 cut(s) 836, 1019
AoxI GGCC 3 cut(s) 1169, 1721, 1861
ApeKI GCWGC 6 cut(s) 724, 889, 1016, 1109, 1777, 1873
ApoI RAATTY 2 cut(s) 1483, 1495
Asp718I GGTACC 1 cut(s) 1830
AspLEI GCGC 2 cut(s) 440, 958
AspS9I GGNCC 3 cut(s) 52, 1208, 1786
AsuHPI GGTGA 4 cut(s) 116, 632, 641, 920
AsuII TTCGAA 2 cut(s) 1569, 1750
AvaII GGWCC 3 cut(s) 52, 1208, 1786
BaeGI GKGCMC 1 cut(s) 1921
BanI GGYRCC 2 cut(s) 1830, 1918
BanII GRGCYC 1 cut(s) 814
BarI GAAGNNNNNNTAC 2 cut(s) 13, 45
BauI CACGAG 1 cut(s) 1626
BbsI GAAGAC 1 cut(s) 1204
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 6 cut(s) 736, 901, 1003, 1096, 1764, 1860
BccI CCATC 7 cut(s) 95, 307, 575, 725, 1548, 1853, 1930
BceAI ACGGC 1 cut(s) 439
BciT130I CCWGG 2 cut(s) 140, 1203
BciVI GTATCC 3 cut(s) 1160, 1465, 1606
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 5 cut(s) 222, 851, 1274, 1421, 1439
BfaI CTAG 4 cut(s) 420, 537, 864, 1926
BfmI CTRYAG 1 cut(s) 722
BfuI GTATCC 3 cut(s) 1160, 1465, 1606
BglI GCCNNNNNGGC 1 cut(s) 1916
BglII AGATCT 1 cut(s) 37
BisI GCNGC 8 cut(s) 725, 769, 890, 1017, 1110, 1778, 1864, 1874
BlsI GCNGC 8 cut(s) 726, 770, 891, 1018, 1111, 1779, 1865, 1875
Bme1390I CCNGG 2 cut(s) 140, 1203
Bme18I GGWCC 3 cut(s) 52, 1208, 1786
BmgBI CACGTC 1 cut(s) 1673
BmgT120I GGNCC 3 cut(s) 52, 1208, 1786
BmiI GGNNCC 4 cut(s) 54, 345, 1832, 1920
BmrFI CCNGG 2 cut(s) 140, 1203
BmsI GCATC 3 cut(s) 1297, 1526, 1860
BpiI GAAGAC 1 cut(s) 1204
BplI GAGNNNNNCTC 4 cut(s) 913, 945, 1229, 1261
BpmI CTGGAG 1 cut(s) 501
Bpu14I TTCGAA 2 cut(s) 1569, 1750
BpuEI CTTGAG 5 cut(s) 18, 110, 243, 1177, 1231
BsaJI CCNNGG 2 cut(s) 1336, 1466
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bsc4I CCNNNNNNNGG 4 cut(s) 727, 1250, 1268, 1353
Bse3DI GCAATG 2 cut(s) 931, 1183
BseBI CCWGG 2 cut(s) 140, 1203
BseDI CCNNGG 2 cut(s) 1336, 1466
BseGI GGATG 5 cut(s) 318, 586, 736, 1541, 1875
BseLI CCNNNNNNNGG 4 cut(s) 727, 1250, 1268, 1353
BseMI GCAATG 2 cut(s) 931, 1183
BseMII CTCAG 3 cut(s) 81, 201, 932
BseRI GAGGAG 5 cut(s) 227, 252, 413, 601, 638
BseSI GKGCMC 1 cut(s) 1921
BseXI GCAGC 6 cut(s) 736, 901, 1003, 1096, 1764, 1860
BseYI CCCAGC 2 cut(s) 1112, 1763
BsgI GTGCAG 1 cut(s) 849
Bsh1236I CGCG 3 cut(s) 440, 855, 958
BshFI GGCC 3 cut(s) 1171, 1723, 1863
BshNI GGYRCC 2 cut(s) 1830, 1918
BsiHKAI GWGCWC 1 cut(s) 126
BslI CCNNNNNNNGG 4 cut(s) 727, 1250, 1268, 1353
BsmAI GTCTC 5 cut(s) 222, 851, 1274, 1421, 1439
BsnI GGCC 3 cut(s) 1171, 1723, 1863
Bsp119I TTCGAA 2 cut(s) 1569, 1750
Bsp1286I GDGCHC 3 cut(s) 126, 814, 1921
Bsp143I GATC 6 cut(s) 25, 37, 283, 688, 1080, 1633
Bsp19I CCATGG 1 cut(s) 1336
BspACI CCGC 6 cut(s) 440, 467, 768, 853, 958, 1864
BspANI GGCC 3 cut(s) 1171, 1723, 1863
BspCNI CTCAG 3 cut(s) 82, 202, 931
BspFNI CGCG 3 cut(s) 440, 855, 958
BspHI TCATGA 3 cut(s) 223, 1065, 1797
BspLI GGNNCC 4 cut(s) 54, 345, 1832, 1920
BspMAI CTGCAG 1 cut(s) 726
BspPI GGATC 1 cut(s) 20
BspQI GCTCTTC 2 cut(s) 204, 819
BspT104I TTCGAA 2 cut(s) 1569, 1750
BspT107I GGYRCC 2 cut(s) 1830, 1918
BsrDI GCAATG 2 cut(s) 931, 1183
BssECI CCNNGG 2 cut(s) 1336, 1466
BssMI GATC 6 cut(s) 25, 37, 283, 688, 1080, 1633
BssSI CACGAG 1 cut(s) 1626
BssT1I CCWWGG 2 cut(s) 1336, 1466
Bst2BI CACGAG 1 cut(s) 1626
Bst2UI CCWGG 2 cut(s) 140, 1203
Bst4CI ACNGT 5 cut(s) 119, 1450, 1463, 1477, 1790
Bst6I CTCTTC 5 cut(s) 204, 225, 819, 901, 1566
BstAPI GCANNNNNTGC 1 cut(s) 886
BstBI TTCGAA 2 cut(s) 1569, 1750
BstC8I GCNNGC 3 cut(s) 722, 967, 1173
BstDEI CTNAG 6 cut(s) 41, 90, 210, 356, 918, 1379
BstDSI CCRYGG 1 cut(s) 1336
BstENI CCTNNNNNAGG 1 cut(s) 725
BstF5I GGATG 5 cut(s) 318, 586, 736, 1541, 1875
BstFNI CGCG 3 cut(s) 440, 855, 958
BstHHI GCGC 2 cut(s) 440, 958
BstKTI GATC 6 cut(s) 28, 40, 286, 691, 1083, 1636
BstMAI GTCTC 5 cut(s) 222, 851, 1274, 1421, 1439
BstMBI GATC 6 cut(s) 25, 37, 283, 688, 1080, 1633
BstNI CCWGG 2 cut(s) 140, 1203
BstSCI CCNGG 2 cut(s) 138, 1201
BstSFI CTRYAG 1 cut(s) 722
BstSLI GKGCMC 1 cut(s) 1921
BstUI CGCG 3 cut(s) 440, 855, 958
BstV1I GCAGC 6 cut(s) 736, 901, 1003, 1096, 1764, 1860
BstV2I GAAGAC 1 cut(s) 1204
BstX2I RGATCY 1 cut(s) 37
BstXI CCANNNNNNTGG 1 cut(s) 509
BstYI RGATCY 1 cut(s) 37
BsuI GTATCC 3 cut(s) 1160, 1465, 1606
BsuRI GGCC 3 cut(s) 1171, 1723, 1863
BtgI CCRYGG 1 cut(s) 1336
BtgZI GCGATG 1 cut(s) 1274
BtrI CACGTC 1 cut(s) 1673
BtsCI GGATG 5 cut(s) 318, 586, 736, 1541, 1875
BtsI GCAGTG 1 cut(s) 1745
BtsIMutI CAGTG 3 cut(s) 124, 207, 1745
Cac8I GCNNGC 3 cut(s) 722, 967, 1173
CaiI CAGNNNCTG 2 cut(s) 836, 1019
CciI TCATGA 3 cut(s) 223, 1065, 1797
CfoI GCGC 2 cut(s) 440, 958
Cfr13I GGNCC 3 cut(s) 52, 1208, 1786
CsiI ACCWGGT 2 cut(s) 138, 1201
Csp6I GTAC 6 cut(s) 13, 130, 915, 1333, 1400, 1831
CviQI GTAC 6 cut(s) 13, 130, 915, 1333, 1400, 1831
DdeI CTNAG 6 cut(s) 41, 90, 210, 356, 918, 1379
DpnI GATC 6 cut(s) 27, 39, 285, 690, 1082, 1635
DpnII GATC 6 cut(s) 25, 37, 283, 688, 1080, 1633
DraI TTTAAA 1 cut(s) 1104
DrdI GACNNNNNNGTC 2 cut(s) 692, 1793
DseDI GACNNNNNNGTC 2 cut(s) 692, 1793
EaeI YGGCCR 1 cut(s) 1861
Eam1104I CTCTTC 5 cut(s) 204, 225, 819, 901, 1566
EarI CTCTTC 5 cut(s) 204, 225, 819, 901, 1566
EciI GGCGGA 1 cut(s) 482
Eco130I CCWWGG 2 cut(s) 1336, 1466
Eco24I GRGCYC 1 cut(s) 814
Eco47I GGWCC 3 cut(s) 52, 1208, 1786
Eco57I CTGAAG 1 cut(s) 1049
EcoNI CCTNNNNNAGG 1 cut(s) 725
EcoRII CCWGG 2 cut(s) 138, 1201
EcoT14I CCWWGG 2 cut(s) 1336, 1466
EcoT22I ATGCAT 1 cut(s) 618
EcoT38I GRGCYC 1 cut(s) 814
ErhI CCWWGG 2 cut(s) 1336, 1466
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 8 cut(s) 725, 769, 890, 1017, 1110, 1778, 1864, 1874
FokI GGATG 5 cut(s) 325, 593, 743, 1548, 1882
FriOI GRGCYC 1 cut(s) 814
Fsp4HI GCNGC 8 cut(s) 725, 769, 890, 1017, 1110, 1778, 1864, 1874
FspBI CTAG 4 cut(s) 420, 537, 864, 1926
GlaI GCGC 2 cut(s) 439, 957
GluI GCNGC 8 cut(s) 725, 769, 890, 1017, 1110, 1778, 1864, 1874
GsaI CCCAGC 2 cut(s) 1116, 1767
GsuI CTGGAG 1 cut(s) 501
HaeIII GGCC 3 cut(s) 1171, 1723, 1863
HhaI GCGC 2 cut(s) 440, 958
Hin6I GCGC 2 cut(s) 438, 956
HinP1I GCGC 2 cut(s) 438, 956
HindIII AAGCTT 2 cut(s) 79, 1041
HinfI GANTC 8 cut(s) 164, 215, 775, 1022, 1272, 1425, 1501, 1747
HphI GGTGA 4 cut(s) 116, 632, 641, 920
Hpy166II GTNNAC 3 cut(s) 52, 1208, 1459
Hpy188I TCNGA 7 cut(s) 115, 640, 702, 819, 921, 1638, 1772
Hpy8I GTNNAC 3 cut(s) 52, 1208, 1459
HpyAV CCTTC 7 cut(s) 39, 754, 784, 1262, 1561, 1848, 1879
HpyCH4III ACNGT 5 cut(s) 119, 1450, 1463, 1477, 1790
HpyCH4IV ACGT 1 cut(s) 1672
HpyF3I CTNAG 6 cut(s) 41, 90, 210, 356, 918, 1379
HpySE526I ACGT 1 cut(s) 1672
HspAI GCGC 2 cut(s) 438, 956
KpnI GGTACC 1 cut(s) 1834
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 6 cut(s) 25, 37, 283, 688, 1080, 1633
LguI GCTCTTC 2 cut(s) 204, 819
LmnI GCTCC 1 cut(s) 707
Lsp1109I GCAGC 6 cut(s) 736, 901, 1003, 1096, 1764, 1860
LweI GCATC 3 cut(s) 1297, 1526, 1860
MabI ACCWGGT 2 cut(s) 138, 1201
MaeI CTAG 4 cut(s) 420, 537, 864, 1926
MaeII ACGT 1 cut(s) 1672
MaeIII GTNAC 2 cut(s) 493, 590
MalI GATC 6 cut(s) 27, 39, 285, 690, 1082, 1635
MboI GATC 6 cut(s) 25, 37, 283, 688, 1080, 1633
MfeI CAATTG 1 cut(s) 428
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 3 cut(s) 126, 814, 1921
MlyI GAGTC 2 cut(s) 224, 1281
MmeI TCCRAC 2 cut(s) 724, 1590
Mph1103I ATGCAT 1 cut(s) 618
MseI TTAA 8 cut(s) 150, 384, 995, 1103, 1317, 1410, 1487, 1530
MslI CAYNNNNRTG 1 cut(s) 525
MspA1I CMGCKG 3 cut(s) 1016, 1777, 1866
MspR9I CCNGG 2 cut(s) 140, 1203
MunI CAATTG 1 cut(s) 428
MvaI CCWGG 2 cut(s) 140, 1203
MvnI CGCG 3 cut(s) 440, 855, 958
NcoI CCATGG 1 cut(s) 1336
NdeII GATC 6 cut(s) 25, 37, 283, 688, 1080, 1633
NlaIV GGNNCC 4 cut(s) 54, 345, 1832, 1920
NsiI ATGCAT 1 cut(s) 618
NspV TTCGAA 2 cut(s) 1569, 1750
PagI TCATGA 3 cut(s) 223, 1065, 1797
PciSI GCTCTTC 2 cut(s) 204, 819
PfeI GAWTC 6 cut(s) 164, 775, 1022, 1425, 1501, 1747
PflMI CCANNNNNTGG 1 cut(s) 1353
PkrI GCNGC 8 cut(s) 726, 770, 891, 1018, 1111, 1779, 1865, 1875
PleI GAGTC 2 cut(s) 223, 1280
PpsI GAGTC 2 cut(s) 223, 1280
Psp6I CCWGG 2 cut(s) 138, 1201
PspFI CCCAGC 2 cut(s) 1112, 1763
PspGI CCWGG 2 cut(s) 138, 1201
PspN4I GGNNCC 4 cut(s) 54, 345, 1832, 1920
PspPI GGNCC 3 cut(s) 52, 1208, 1786
PstI CTGCAG 1 cut(s) 726
PstNI CAGNNNCTG 2 cut(s) 836, 1019
PsuI RGATCY 1 cut(s) 37
PvuII CAGCTG 2 cut(s) 1016, 1777
RsaI GTAC 6 cut(s) 14, 131, 916, 1334, 1401, 1832
RsaNI GTAC 6 cut(s) 13, 130, 915, 1333, 1400, 1831
RseI CAYNNNNRTG 1 cut(s) 525
SapI GCTCTTC 2 cut(s) 204, 819
SaqAI TTAA 8 cut(s) 150, 384, 995, 1103, 1317, 1410, 1487, 1530
SatI GCNGC 8 cut(s) 725, 769, 890, 1017, 1110, 1778, 1864, 1874
Sau3AI GATC 6 cut(s) 25, 37, 283, 688, 1080, 1633
Sau96I GGNCC 3 cut(s) 52, 1208, 1786
SchI GAGTC 2 cut(s) 224, 1281
ScrFI CCNGG 2 cut(s) 140, 1203
SduI GDGCHC 3 cut(s) 126, 814, 1921
SexAI ACCWGGT 2 cut(s) 138, 1201
SfaNI GCATC 3 cut(s) 1297, 1526, 1860
SfcI CTRYAG 1 cut(s) 722
SfuI TTCGAA 2 cut(s) 1569, 1750
SinI GGWCC 3 cut(s) 52, 1208, 1786
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 5 cut(s) 33, 125, 258, 1192, 1246
SmoI CTYRAG 5 cut(s) 33, 125, 258, 1192, 1246
SsiI CCGC 6 cut(s) 440, 467, 768, 853, 958, 1864
SspMI CTAG 4 cut(s) 420, 537, 864, 1926
StyD4I CCNGG 2 cut(s) 138, 1201
StyI CCWWGG 2 cut(s) 1336, 1466
TaaI ACNGT 5 cut(s) 119, 1450, 1463, 1477, 1790
TaiI ACGT 1 cut(s) 1675
TaqI TCGA 3 cut(s) 1258, 1569, 1750
TatI WGTACW 2 cut(s) 129, 914
TauI GCSGC 2 cut(s) 771, 1866
TfiI GAWTC 6 cut(s) 164, 775, 1022, 1425, 1501, 1747
Tru1I TTAA 8 cut(s) 150, 384, 995, 1103, 1317, 1410, 1487, 1530
Tru9I TTAA 8 cut(s) 150, 384, 995, 1103, 1317, 1410, 1487, 1530
TscAI CASTG 3 cut(s) 124, 214, 1745
TseI GCWGC 6 cut(s) 724, 889, 1016, 1109, 1777, 1873
TspGWI ACGGA 1 cut(s) 1142
TspRI CASTG 3 cut(s) 124, 214, 1745
Van91I CCANNNNNTGG 1 cut(s) 1353
VpaK11BI GGWCC 3 cut(s) 52, 1208, 1786
XagI CCTNNNNNAGG 1 cut(s) 725
XapI RAATTY 2 cut(s) 1483, 1495
XbaI TCTAGA 1 cut(s) 536
XspI CTAG 4 cut(s) 420, 537, 864, 1926
Zsp2I ATGCAT 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.