RchiOBHm_Chr5g0065561

oxidosqualene cyclase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000624
Physical Location & Seq
Forward (+)
71533965 .. 71536819
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34142

Sequence Viewer

Length: 2190 bp
ATGGATCCACGTTTCTCTGAATTTCCCAATTTCAATGATCAAACCCTTTTGCCCGATATGTTGAATGAGTACCCAAGTAGTGACCCTTTTGAAGATCTCAGCTTTTCGGACCCAAACCCCAGTAACTCTGCTCTATCATCTAGCTTTAGCCCCGAGGGAGATGATGTTGAGTTTTCTGATAGTGTGCTCAAGTACATAAACCAGGTGCTTATGGAGGAGGACATGGAGTCAAAGCCATGTATGTTCCCTGATCCTTTGGCTCTTCAAGCCACTGAGGAGTCTCTTTATGAGGTTATTGGGGGAGGGAAGTACCCAATTTCCCAAGACCAAAACCCTCTTTGTGTTGATCAAAATGTTCAGAGCCCAGATGGCGGTTATTTTTCTGGAAGTTCCAGTGATTCTAGCTCCAGTCCTGGTTCTTCCGTTGACTCTTGCCCGGTCACTGCTCAGTTTGTAGGGACTAGGAGCTCTATATTGCAAAACCCTATTCCTGAAAACTTTGTTTTCCAGTCCCCTTCCAGGGCTAGGTCTCAATCGTCGTCGGATGGGTATGGCAATGGGAATGGGATGGTGGCTTCTTATAGGAGTGAGCTAATGGTTCCTGATGTGCTTAGTAAGAATGAATTGTTAGTGCAGTTTAACCTAGGGGCAGAGGAGGGTAGTAAGTTTCTTCCTAGAGGTCAATTGATTAGTGCGGAGGGCAACAAGATGTATGCTGTGGTGAATAAAAAGGCTGGAGATGTGGTAGTAACAACGCAGAAGAATGGGAGTGAGCAATTTCATGTTGGTTCAAGAGGAAAAAAGAATCATCAAAGGGAAGATATAGATTTGGAGGATGGGAGGAGTAACAAGCAACCAGCAGTTTTTATGGACGATGAGGAGGGTGAGCTGTCTGATATTTTTGACAAGGTGTTGCTCTGTAAGCCTTTGGAGAGTAAAGACGATGAAGCCAGTCAGAATGGAGCAAACAATGGCCTGCAGCAGGATGTGCAATCTGTTGGAATTGGTAATGGGAAGGGTCGCGGCAAGATTCACAGCAACAAGAAGGAAGTGATAGATTTGAGGGCTCTTCTGATTTTATGTGCACAAGCTGTTTCTGTTGATGATCGCGAGACTGCTAGTGAGCTACTAAAGCAGATTATGCAGCACTCTTCCCCATTTGGTGATTGTGCTCAGAGGTTGGCTCATTGCTTTGCAAATGCTCTTGAAGCGCGGTTAGCTGGCACTGGAACTCAAATATATACTGCTTTATGTGCTAAACGAATGTCAGCTGCTGATATGCTGAAGTTTTATCAAGCTTATATTGCAGCCTGCCCATTCATGAAGATGTCCATGATCTTTGCAAACCATATGATTTTAAAAGCAGCTGAGAAAGCAGAAACACTTCATATAATAGATTTTGGTATCCTTTATGGTTTTCAGTGGCCTGCTCTCATTCATTGCCTTTCAAGAAGACCTGGTGGACCTCCCAAATTACGTATTACAGGGGTAGAGCTCCCCTTAAGTGGTTTTCGACCAAAAGAAAGAGTCCAAGAGACAGGGCATCGCTTGGCAAAGTATTGTAAGCGATTTAATGTTCCTTTTGAGTACATTGCCATAGCCAAAAAATGGGAAACTGTCCAATTTGATGACCTCAAAGTTCAAAGAAATGAGGTGCTTGCTGTTAATTGTCTCTGCCGATTTAAGAACCTCCTTGATGAGACCGTTGTGGTAAACAGTCCAAGGGATACTGTTCTAAATTTAATTAGGAAGTTAAATCCTGATGTCTTTGTCCAAAGTGTTGTTAATGGATCCTATCATGCACCCTTCTTTGTTTCACGCTTCCGAGAGGCACTATTCCACTTCTCTGCATTGTTTGATATGTTTGATAACAATTTGCCTCGTGAAGATCTGATGAGATTAGGGTTTGAGAAAGAGTTTATTGGACGTGAAGTTGTGAATATAGTAGCTTGTGAGGGTTCAGAAAGAACTGTGAGGCCAGAAACATATAAGCAGTGGCAGGTTCGGAACATGAGGGCTGGGTTCAGACAGCTGCCCTTGGACCGTGAAGTCATGAACAAAATAAAGGATAAAGTAAAAACGGGGTACAGCCCTGATTTTGTGGTTGATGAGGATGGCCGCTGGATGCTGCAGGGATGGAAGGGCAGAATTATGTTTTGTTCCGCCTGTTGGGCATTGCCTAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 map00100 map00909 map01100 map01110
Pfam Domains
Protein Families
Prenyltrans SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

729

Amino Acids

81.51

Weight (kDa)

5.43

Isoelectric Point (pI)

47.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 354 - 724 2.4e-117 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2048
Acc36I ACCTGC 1 cut(s) 1990
AccB7I CCANNNNNTGG 1 cut(s) 1608
AccII CGCG 3 cut(s) 1023, 1110, 1213
AciI CCGC 6 cut(s) 372, 695, 1023, 1213, 2119, 2163
AclWI GGATC 4 cut(s) 12, 245, 1785, 1798
AcoI YGGCCR 1 cut(s) 2116
AcsI RAATTY 2 cut(s) 20, 1738
AcuI CTGAAG 1 cut(s) 1304
AfaI GTAC 5 cut(s) 71, 194, 311, 1589, 2087
AfiI CCNNNNNNNGG 8 cut(s) 371, 519, 520, 525, 982, 1505, 1608, 2169
AflII CTTAAG 1 cut(s) 1501
AgsI TTSAA 8 cut(s) 34, 64, 92, 266, 792, 1208, 1449, 1643
AhdI GACNNNNNGTC 1 cut(s) 226
AjiI CACGTC 1 cut(s) 1928
AjnI CCWGG 4 cut(s) 201, 412, 518, 1456
Alw21I GWGCWC 5 cut(s) 189, 470, 1087, 1174, 1497
Alw26I GTCTC 6 cut(s) 285, 534, 1106, 1529, 1676, 1694
Alw44I GTGCAC 1 cut(s) 1083
AlwI GGATC 4 cut(s) 12, 245, 1785, 1798
AlwNI CAGNNNCTG 1 cut(s) 1274
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 4 cut(s) 973, 1424, 1976, 2116
ApaLI GTGCAC 1 cut(s) 1083
ApeKI GCWGC 7 cut(s) 979, 1144, 1271, 1307, 1364, 2032, 2128
ApoI RAATTY 2 cut(s) 20, 1738
Asp700I GAANNNNTTC 1 cut(s) 1383
AspA2I CCTAGG 2 cut(s) 643, 2180
AspLEI GCGC 1 cut(s) 1213
AspS9I GGNCC 3 cut(s) 109, 1463, 2041
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 3 cut(s) 733, 896, 1175
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 3 cut(s) 109, 1463, 2041
AvrII CCTAGG 2 cut(s) 643, 2180
BaeGI GKGCMC 1 cut(s) 1087
BamHI GGATCC 2 cut(s) 4, 1790
BanII GRGCYC 4 cut(s) 365, 470, 1069, 1497
BauI CACGAG 1 cut(s) 1881
BbsI GAAGAC 1 cut(s) 1459
Bbv12I GWGCWC 5 cut(s) 189, 470, 1087, 1174, 1497
BbvI GCAGC 7 cut(s) 991, 1156, 1258, 1319, 1376, 2019, 2115
BccI CCATC 6 cut(s) 362, 539, 562, 830, 2108, 2130
BciT130I CCWGG 4 cut(s) 203, 414, 520, 1458
BciVI GTATCC 2 cut(s) 1415, 1720
BclI TGATCA 2 cut(s) 37, 346
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 6 cut(s) 285, 534, 1106, 1529, 1676, 1694
BfaI CTAG 8 cut(s) 141, 402, 462, 525, 644, 675, 1119, 2181
BfmI CTRYAG 2 cut(s) 977, 2129
BfrI CTTAAG 1 cut(s) 1501
BfuAI ACCTGC 1 cut(s) 1990
BfuI GTATCC 2 cut(s) 1415, 1720
BglI GCCNNNNNGGC 2 cut(s) 369, 2171
BglII AGATCT 2 cut(s) 94, 1888
BisI GCNGC 9 cut(s) 980, 1024, 1145, 1272, 1308, 1365, 2033, 2119, 2129
BlnI CCTAGG 2 cut(s) 643, 2180
BlsI GCNGC 9 cut(s) 981, 1025, 1146, 1273, 1309, 1366, 2034, 2120, 2130
Bme1390I CCNGG 5 cut(s) 203, 414, 437, 520, 1458
Bme18I GGWCC 3 cut(s) 109, 1463, 2041
BmeRI GACNNNNNGTC 1 cut(s) 226
BmeT110I CYCGRG 1 cut(s) 152
BmgBI CACGTC 1 cut(s) 1928
BmgT120I GGNCC 3 cut(s) 109, 1463, 2041
BmiI GGNNCC 4 cut(s) 6, 111, 600, 1792
BmrFI CCNGG 5 cut(s) 203, 414, 437, 520, 1458
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 2 cut(s) 1552, 2115
BmuI ACTGGG 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 1459
BplI GAGNNNNNCTC 2 cut(s) 1168, 1200
BpmI CTGGAG 2 cut(s) 391, 756
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 437
BsaAI YACGTR 1 cut(s) 1478
BsaI GGTCTC 2 cut(s) 534, 1694
BsaJI CCNNGG 6 cut(s) 153, 519, 643, 1721, 2037, 2180
BsaXI ACNNNNNCTCC 4 cut(s) 150, 180, 729, 759
Bsc4I CCNNNNNNNGG 8 cut(s) 371, 519, 520, 525, 982, 1505, 1608, 2169
Bse1I ACTGG 6 cut(s) 120, 393, 408, 508, 951, 1231
Bse3DI GCAATG 5 cut(s) 562, 1186, 1438, 1590, 2174
BseBI CCWGG 4 cut(s) 203, 414, 520, 1458
BseDI CCNNGG 6 cut(s) 153, 519, 643, 1721, 2037, 2180
BseGI GGATG 7 cut(s) 550, 573, 841, 991, 2119, 2130, 2141
BseLI CCNNNNNNNGG 8 cut(s) 371, 519, 520, 525, 982, 1505, 1608, 2169
BseMI GCAATG 5 cut(s) 562, 1186, 1438, 1590, 2174
BseMII CTCAG 5 cut(s) 112, 264, 461, 1187, 1359
BseNI ACTGG 6 cut(s) 120, 393, 408, 508, 951, 1231
BseRI GAGGAG 5 cut(s) 230, 290, 668, 856, 893
BseSI GKGCMC 1 cut(s) 1087
BseXI GCAGC 7 cut(s) 991, 1156, 1258, 1319, 1376, 2019, 2115
BseYI CCCAGC 1 cut(s) 2018
BsgI GTGCAG 1 cut(s) 653
Bsh1236I CGCG 3 cut(s) 1023, 1110, 1213
BshFI GGCC 4 cut(s) 975, 1426, 1978, 2118
BsiHKAI GWGCWC 5 cut(s) 189, 470, 1087, 1174, 1497
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 1 cut(s) 437
BslFI GGGAC 2 cut(s) 472, 496
BslI CCNNNNNNNGG 8 cut(s) 371, 519, 520, 525, 982, 1505, 1608, 2169
BsmAI GTCTC 6 cut(s) 285, 534, 1106, 1529, 1676, 1694
BsmFI GGGAC 2 cut(s) 472, 496
BsnI GGCC 4 cut(s) 975, 1426, 1978, 2118
Bso31I GGTCTC 2 cut(s) 534, 1694
BsoBI CYCGRG 1 cut(s) 152
Bsp1286I GDGCHC 7 cut(s) 189, 365, 470, 1069, 1087, 1174, 1497
Bsp143I GATC 9 cut(s) 4, 37, 94, 250, 346, 1105, 1335, 1790, 1888
Bsp68I TCGCGA 1 cut(s) 1110
BspACI CCGC 6 cut(s) 372, 695, 1023, 1213, 2119, 2163
BspANI GGCC 4 cut(s) 975, 1426, 1978, 2118
BspCNI CTCAG 5 cut(s) 111, 265, 460, 1186, 1360
BspFNI CGCG 3 cut(s) 1023, 1110, 1213
BspHI TCATGA 2 cut(s) 1320, 2052
BspLI GGNNCC 4 cut(s) 6, 111, 600, 1792
BspMAI CTGCAG 2 cut(s) 981, 2133
BspMI ACCTGC 1 cut(s) 1990
BspPI GGATC 4 cut(s) 12, 245, 1785, 1798
BspQI GCTCTTC 2 cut(s) 267, 1074
BspTI CTTAAG 1 cut(s) 1501
BspTNI GGTCTC 2 cut(s) 534, 1694
BsrDI GCAATG 5 cut(s) 562, 1186, 1438, 1590, 2174
BsrI ACTGG 6 cut(s) 120, 393, 408, 508, 951, 1231
BssECI CCNNGG 6 cut(s) 153, 519, 643, 1721, 2037, 2180
BssMI GATC 9 cut(s) 4, 37, 94, 250, 346, 1105, 1335, 1790, 1888
BssSI CACGAG 1 cut(s) 1881
BssT1I CCWWGG 4 cut(s) 643, 1721, 2037, 2180
Bst2BI CACGAG 1 cut(s) 1881
Bst2UI CCWGG 4 cut(s) 203, 414, 520, 1458
Bst4CI ACNGT 6 cut(s) 1618, 1705, 1718, 1732, 1972, 2045
Bst6I CTCTTC 3 cut(s) 267, 1074, 1156
BstAFI CTTAAG 1 cut(s) 1501
BstAPI GCANNNNNTGC 2 cut(s) 988, 1141
BstBAI YACGTR 1 cut(s) 1478
BstC8I GCNNGC 5 cut(s) 977, 1222, 1312, 1428, 1659
BstDEI CTNAG 6 cut(s) 98, 273, 447, 611, 1173, 1368
BstENI CCTNNNNNAGG 1 cut(s) 980
BstF5I GGATG 7 cut(s) 550, 573, 841, 991, 2119, 2130, 2141
BstFNI CGCG 3 cut(s) 1023, 1110, 1213
BstHHI GCGC 1 cut(s) 1213
BstKTI GATC 9 cut(s) 7, 40, 97, 253, 349, 1108, 1338, 1793, 1891
BstMAI GTCTC 6 cut(s) 285, 534, 1106, 1529, 1676, 1694
BstMBI GATC 9 cut(s) 4, 37, 94, 250, 346, 1105, 1335, 1790, 1888
BstNI CCWGG 4 cut(s) 203, 414, 520, 1458
BstSCI CCNGG 5 cut(s) 201, 412, 435, 518, 1456
BstSFI CTRYAG 2 cut(s) 977, 2129
BstSLI GKGCMC 1 cut(s) 1087
BstSNI TACGTA 1 cut(s) 1478
BstUI CGCG 3 cut(s) 1023, 1110, 1213
BstV1I GCAGC 7 cut(s) 991, 1156, 1258, 1319, 1376, 2019, 2115
BstV2I GAAGAC 1 cut(s) 1459
BstX2I RGATCY 4 cut(s) 4, 94, 1790, 1888
BstYI RGATCY 4 cut(s) 4, 94, 1790, 1888
BsuI GTATCC 2 cut(s) 1415, 1720
BsuRI GGCC 4 cut(s) 975, 1426, 1978, 2118
BtgZI GCGATG 1 cut(s) 1529
BtrI CACGTC 1 cut(s) 1928
BtsCI GGATG 7 cut(s) 550, 573, 841, 991, 2119, 2130, 2141
BtsI GCAGTG 2 cut(s) 441, 2000
BtsIMutI CAGTG 6 cut(s) 270, 400, 441, 1224, 1427, 2000
BtuMI TCGCGA 1 cut(s) 1110
BveI ACCTGC 1 cut(s) 1990
Cac8I GCNNGC 5 cut(s) 977, 1222, 1312, 1428, 1659
CaiI CAGNNNCTG 1 cut(s) 1274
CciI TCATGA 2 cut(s) 1320, 2052
CfoI GCGC 1 cut(s) 1213
Cfr13I GGNCC 3 cut(s) 109, 1463, 2041
CsiI ACCWGGT 2 cut(s) 201, 1456
Csp6I GTAC 5 cut(s) 70, 193, 310, 1588, 2086
CviAII CATG 8 cut(s) 223, 237, 782, 1321, 1333, 1799, 2011, 2053
CviQI GTAC 5 cut(s) 70, 193, 310, 1588, 2086
DdeI CTNAG 6 cut(s) 98, 273, 447, 611, 1173, 1368
DpnI GATC 9 cut(s) 6, 39, 96, 252, 348, 1107, 1337, 1792, 1890
DpnII GATC 9 cut(s) 4, 37, 94, 250, 346, 1105, 1335, 1790, 1888
DraI TTTAAA 1 cut(s) 1359
DrdI GACNNNNNNGTC 1 cut(s) 2048
DriI GACNNNNNGTC 1 cut(s) 226
DseDI GACNNNNNNGTC 1 cut(s) 2048
EaeI YGGCCR 1 cut(s) 2116
Eam1104I CTCTTC 3 cut(s) 267, 1074, 1156
Eam1105I GACNNNNNGTC 1 cut(s) 226
EarI CTCTTC 3 cut(s) 267, 1074, 1156
EciI GGCGGA 1 cut(s) 2152
Ecl136II GAGCTC 2 cut(s) 468, 1495
Eco105I TACGTA 1 cut(s) 1478
Eco130I CCWWGG 4 cut(s) 643, 1721, 2037, 2180
Eco24I GRGCYC 4 cut(s) 365, 470, 1069, 1497
Eco31I GGTCTC 2 cut(s) 534, 1694
Eco47I GGWCC 3 cut(s) 109, 1463, 2041
Eco53kI GAGCTC 2 cut(s) 468, 1495
Eco57I CTGAAG 1 cut(s) 1304
Eco88I CYCGRG 1 cut(s) 152
EcoICRI GAGCTC 2 cut(s) 468, 1495
EcoNI CCTNNNNNAGG 1 cut(s) 980
EcoRII CCWGG 4 cut(s) 201, 412, 518, 1456
EcoT14I CCWWGG 4 cut(s) 643, 1721, 2037, 2180
EcoT38I GRGCYC 4 cut(s) 365, 470, 1069, 1497
ErhI CCWWGG 4 cut(s) 643, 1721, 2037, 2180
FaeI CATG 8 cut(s) 226, 240, 785, 1324, 1336, 1802, 2014, 2056
FaqI GGGAC 2 cut(s) 472, 496
FatI CATG 8 cut(s) 222, 236, 781, 1320, 1332, 1798, 2010, 2052
FauNDI CATATG 1 cut(s) 1350
FbaI TGATCA 2 cut(s) 37, 346
Fnu4HI GCNGC 9 cut(s) 980, 1024, 1145, 1272, 1308, 1365, 2033, 2119, 2129
FokI GGATG 7 cut(s) 557, 580, 848, 998, 2126, 2137, 2148
FriOI GRGCYC 4 cut(s) 365, 470, 1069, 1497
Fsp4HI GCNGC 9 cut(s) 980, 1024, 1145, 1272, 1308, 1365, 2033, 2119, 2129
FspBI CTAG 8 cut(s) 141, 402, 462, 525, 644, 675, 1119, 2181
GlaI GCGC 1 cut(s) 1212
GluI GCNGC 9 cut(s) 980, 1024, 1145, 1272, 1308, 1365, 2033, 2119, 2129
GsaI CCCAGC 1 cut(s) 2022
GsuI CTGGAG 2 cut(s) 391, 756
HaeIII GGCC 4 cut(s) 975, 1426, 1978, 2118
HapII CCGG 1 cut(s) 437
HhaI GCGC 1 cut(s) 1213
Hin1II CATG 8 cut(s) 226, 240, 785, 1324, 1336, 1802, 2014, 2056
Hin6I GCGC 1 cut(s) 1211
HinP1I GCGC 1 cut(s) 1211
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 1296
HinfI GANTC 7 cut(s) 227, 278, 398, 428, 805, 1030, 1527
HpaII CCGG 1 cut(s) 437
HphI GGTGA 3 cut(s) 733, 896, 1175
Hpy166II GTNNAC 4 cut(s) 427, 1085, 1463, 1714
Hpy8I GTNNAC 4 cut(s) 427, 1085, 1463, 1714
Hpy99I CGWCG 2 cut(s) 541, 544
HpyAV CCTTC 5 cut(s) 525, 1009, 1039, 1816, 2134
HpyCH4III ACNGT 6 cut(s) 1618, 1705, 1718, 1732, 1972, 2045
HpyCH4IV ACGT 3 cut(s) 10, 1477, 1927
HpyF3I CTNAG 6 cut(s) 98, 273, 447, 611, 1173, 1368
HpySE526I ACGT 3 cut(s) 10, 1477, 1927
Hsp92II CATG 8 cut(s) 226, 240, 785, 1324, 1336, 1802, 2014, 2056
HspAI GCGC 1 cut(s) 1211
Ksp22I TGATCA 2 cut(s) 37, 346
Kzo9I GATC 9 cut(s) 4, 37, 94, 250, 346, 1105, 1335, 1790, 1888
LguI GCTCTTC 2 cut(s) 267, 1074
LmnI GCTCC 4 cut(s) 410, 465, 962, 1500
Lsp1109I GCAGC 7 cut(s) 991, 1156, 1258, 1319, 1376, 2019, 2115
LweI GCATC 2 cut(s) 1552, 2115
MabI ACCWGGT 2 cut(s) 201, 1456
MaeI CTAG 8 cut(s) 141, 402, 462, 525, 644, 675, 1119, 2181
MaeII ACGT 3 cut(s) 10, 1477, 1927
MaeIII GTNAC 5 cut(s) 80, 122, 439, 748, 845
MalI GATC 9 cut(s) 6, 39, 96, 252, 348, 1107, 1337, 1792, 1890
MboI GATC 9 cut(s) 4, 37, 94, 250, 346, 1105, 1335, 1790, 1888
MfeI CAATTG 1 cut(s) 683
MflI RGATCY 4 cut(s) 4, 94, 1790, 1888
MhlI GDGCHC 7 cut(s) 189, 365, 470, 1069, 1087, 1174, 1497
MlyI GAGTC 4 cut(s) 236, 287, 422, 1536
MmeI TCCRAC 2 cut(s) 522, 979
MroXI GAANNNNTTC 1 cut(s) 1383
MseI TTAA 9 cut(s) 639, 1358, 1502, 1572, 1665, 1683, 1742, 1754, 1785
MslI CAYNNNNRTG 1 cut(s) 1325
MspA1I CMGCKG 4 cut(s) 1271, 1367, 2032, 2121
MspCI CTTAAG 1 cut(s) 1501
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 5 cut(s) 203, 414, 437, 520, 1458
MunI CAATTG 1 cut(s) 683
MvaI CCWGG 4 cut(s) 203, 414, 520, 1458
MvnI CGCG 3 cut(s) 1023, 1110, 1213
NciI CCSGG 1 cut(s) 437
NdeI CATATG 1 cut(s) 1350
NdeII GATC 9 cut(s) 4, 37, 94, 250, 346, 1105, 1335, 1790, 1888
NlaIII CATG 8 cut(s) 226, 240, 785, 1324, 1336, 1802, 2014, 2056
NlaIV GGNNCC 4 cut(s) 6, 111, 600, 1792
NmuCI GTSAC 2 cut(s) 80, 439
NruI TCGCGA 1 cut(s) 1110
PagI TCATGA 2 cut(s) 1320, 2052
PciSI GCTCTTC 2 cut(s) 267, 1074
PdmI GAANNNNTTC 1 cut(s) 1383
PfeI GAWTC 3 cut(s) 398, 805, 1030
PflMI CCANNNNNTGG 1 cut(s) 1608
PkrI GCNGC 9 cut(s) 981, 1025, 1146, 1273, 1309, 1366, 2034, 2120, 2130
PleI GAGTC 4 cut(s) 235, 286, 422, 1535
PpsI GAGTC 4 cut(s) 235, 286, 422, 1535
Ppu21I YACGTR 1 cut(s) 1478
Psp124BI GAGCTC 2 cut(s) 470, 1497
Psp6I CCWGG 4 cut(s) 201, 412, 518, 1456
PspFI CCCAGC 1 cut(s) 2018
PspGI CCWGG 4 cut(s) 201, 412, 518, 1456
PspN4I GGNNCC 4 cut(s) 6, 111, 600, 1792
PspPI GGNCC 3 cut(s) 109, 1463, 2041
PstI CTGCAG 2 cut(s) 981, 2133
PstNI CAGNNNCTG 1 cut(s) 1274
PsuI RGATCY 4 cut(s) 4, 94, 1790, 1888
PvuII CAGCTG 3 cut(s) 1271, 1367, 2032
RruI TCGCGA 1 cut(s) 1110
RsaI GTAC 5 cut(s) 71, 194, 311, 1589, 2087
RsaNI GTAC 5 cut(s) 70, 193, 310, 1588, 2086
RseI CAYNNNNRTG 1 cut(s) 1325
SacI GAGCTC 2 cut(s) 470, 1497
SapI GCTCTTC 2 cut(s) 267, 1074
SaqAI TTAA 9 cut(s) 639, 1358, 1502, 1572, 1665, 1683, 1742, 1754, 1785
SatI GCNGC 9 cut(s) 980, 1024, 1145, 1272, 1308, 1365, 2033, 2119, 2129
Sau3AI GATC 9 cut(s) 4, 37, 94, 250, 346, 1105, 1335, 1790, 1888
Sau96I GGNCC 3 cut(s) 109, 1463, 2041
SchI GAGTC 4 cut(s) 236, 287, 422, 1536
ScrFI CCNGG 5 cut(s) 203, 414, 437, 520, 1458
SduI GDGCHC 7 cut(s) 189, 365, 470, 1069, 1087, 1174, 1497
SexAI ACCWGGT 2 cut(s) 201, 1456
SfaNI GCATC 2 cut(s) 1552, 2115
SfcI CTRYAG 2 cut(s) 977, 2129
SinI GGWCC 3 cut(s) 109, 1463, 2041
SmiMI CAYNNNNRTG 1 cut(s) 1325
SmlI CTYRAG 2 cut(s) 188, 1501
SmoI CTYRAG 2 cut(s) 188, 1501
SnaBI TACGTA 1 cut(s) 1478
SsiI CCGC 6 cut(s) 372, 695, 1023, 1213, 2119, 2163
SspMI CTAG 8 cut(s) 141, 402, 462, 525, 644, 675, 1119, 2181
SstI GAGCTC 2 cut(s) 470, 1497
StyD4I CCNGG 5 cut(s) 201, 412, 435, 518, 1456
StyI CCWWGG 4 cut(s) 643, 1721, 2037, 2180
TaaI ACNGT 6 cut(s) 1618, 1705, 1718, 1732, 1972, 2045
TaiI ACGT 3 cut(s) 13, 1480, 1930
TaqI TCGA 1 cut(s) 1513
TatI WGTACW 2 cut(s) 192, 1587
TauI GCSGC 2 cut(s) 1026, 2121
TfiI GAWTC 3 cut(s) 398, 805, 1030
Tru1I TTAA 9 cut(s) 639, 1358, 1502, 1572, 1665, 1683, 1742, 1754, 1785
Tru9I TTAA 9 cut(s) 639, 1358, 1502, 1572, 1665, 1683, 1742, 1754, 1785
TscAI CASTG 6 cut(s) 277, 400, 448, 1231, 1427, 2000
TseFI GTSAC 2 cut(s) 80, 439
TseI GCWGC 7 cut(s) 979, 1144, 1271, 1307, 1364, 2032, 2128
Tsp45I GTSAC 2 cut(s) 80, 439
TspDTI ATGAA 8 cut(s) 636, 770, 960, 1309, 1337, 1376, 1427, 2069
TspGWI ACGGA 1 cut(s) 412
TspRI CASTG 6 cut(s) 277, 400, 448, 1231, 1427, 2000
Van91I CCANNNNNTGG 1 cut(s) 1608
Vha464I CTTAAG 1 cut(s) 1501
VneI GTGCAC 1 cut(s) 1083
VpaK11BI GGWCC 3 cut(s) 109, 1463, 2041
XagI CCTNNNNNAGG 1 cut(s) 980
XapI RAATTY 2 cut(s) 20, 1738
XmaJI CCTAGG 2 cut(s) 643, 2180
XmnI GAANNNNTTC 1 cut(s) 1383
XspI CTAG 8 cut(s) 141, 402, 462, 525, 644, 675, 1119, 2181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.