FvH4_4g06830
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002334
Physical Location & Seq
Reverse (-)
6132248 .. 6133996
1749 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06830.t2

Sequence Viewer

Length: 906 bp
ATGGCAATTGATCTTGTTAGGTTCTCCAAGATCGACGATCGGACGGCCATGGAAGAAGCCGCCTCCGCCGGTTTGCAGAGCATGGAGCACCTTATCCGCGTCTTGTCAAGCCAAACCCCGTCTCAGAACAACCTCGATTGCCGTGAAATCACCGACTTCACCGTCTCCAAGTTCAAGCAGTTCATCTCCGTCTTGAACCGGACCGGCCACGCCCGCTTCCGCCGCGGACCGGCTAAGCAACCTAGTGAACCGACAACCACCTATTCAGCTCCTCCACCTCCTCCCAACCCGGTCCCACCAAAGTCTCAGACCACTTTGTCTTTTGAATCCAGAAATCCCAAAGCAAACACCAAAACCAATTCCAACTCCAAAGACTGCCTCACCTTGTCTCCACCCATGTCAACTACGTCGTCGTTTCTTTCTTCCGTCACCACCGGAGACGGCAGCATTTCGAACGGGAAAGTCTTCTCATCAGCTGCCGTAGCCCCGGCCCCGGCCTTCTCCGCCGGTAAGCCTCCGCTCTCACAATCTCACCGGAAACGCTGCCACGACGAGCTGGCGACCAAAACTACATCCTCCGGCCGATGCCATTGTTCTAAGAGAAGAAAAACCAAGGTGAAGAGGACAGTCCGTGTGCCGGCGATTAGTTCCAAAATCGCCGATATACCTTCCGACGAGTTTTCATGGAGGAAGTACGGCCAAAAGCCGATCAAGGGCTCCCCTTACCCACGAGGCTATTATAAGTGTAGTAGCGTGCGAGGGTGCCCAGCGAGGAAACACGTGGAGCGCGCACAGGACGACCCCACCATGCTTATCGTTACCTACGAAGCCGAGCACCGTCACCCTCACCCGCAGTCTAACTCAGTCCCCGCCGGTGTCGTGGGTCTCGTGTTCCAGTCGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plant_zn_clust WRKY

Protein Analysis

302

Amino Acids

32.9

Weight (kDa)

9.97

Isoelectric Point (pI)

53.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_zn_clust PF10533 179 - 221 1.2e-17 Plant zinc cluster domain
WRKY PF03106 225 - 244 3.2e-06 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 741
AasI GACNNNNNNGTC 2 cut(s) 161, 316
AccB1I GGYRCC 1 cut(s) 762
AccBSI CCGCTC 1 cut(s) 520
AccII CGCG 3 cut(s) 99, 225, 789
AcoI YGGCCR 4 cut(s) 45, 205, 580, 697
AcvI CACGTG 1 cut(s) 781
AfaI GTAC 1 cut(s) 695
AfiI CCNNNNNNNGG 4 cut(s) 229, 493, 637, 713
AflIII ACRYGT 1 cut(s) 778
AgsI TTSAA 3 cut(s) 175, 196, 326
AluBI AGCT 3 cut(s) 269, 476, 556
AluI AGCT 3 cut(s) 269, 476, 556
Alw21I GWGCWC 2 cut(s) 90, 837
Alw26I GTCTC 6 cut(s) 126, 169, 309, 393, 432, 890
AoxI GGCC 6 cut(s) 45, 205, 489, 495, 580, 697
ApeKI GCWGC 3 cut(s) 444, 476, 543
Asp700I GAANNNNTTC 1 cut(s) 464
AspLEI GCGC 2 cut(s) 789, 791
AspS9I GGNCC 4 cut(s) 201, 227, 292, 490
AsuC2I CCSGG 3 cut(s) 290, 488, 494
AsuHPI GGTGA 8 cut(s) 142, 151, 373, 421, 524, 628, 833, 839
AsuII TTCGAA 1 cut(s) 452
AvaII GGWCC 3 cut(s) 201, 227, 292
BaeGI GKGCMC 1 cut(s) 767
BanI GGYRCC 1 cut(s) 762
BanII GRGCYC 1 cut(s) 719
BauI CACGAG 2 cut(s) 729, 887
BbrPI CACGTG 1 cut(s) 781
BbsI GAAGAC 1 cut(s) 457
Bbv12I GWGCWC 2 cut(s) 90, 837
BbvI GCAGC 3 cut(s) 456, 463, 530
BceAI ACGGC 5 cut(s) 60, 126, 457, 464, 712
BcnI CCSGG 3 cut(s) 290, 488, 494
BcoDI GTCTC 6 cut(s) 126, 169, 309, 393, 432, 890
BfaI CTAG 1 cut(s) 243
BisI GCNGC 5 cut(s) 60, 223, 445, 477, 544
BlpI GCTNAGC 1 cut(s) 234
BlsI GCNGC 5 cut(s) 61, 224, 446, 478, 545
Bme1390I CCNGG 3 cut(s) 290, 488, 494
Bme18I GGWCC 3 cut(s) 201, 227, 292
BmgT120I GGNCC 4 cut(s) 201, 227, 292, 490
BmiI GGNNCC 4 cut(s) 294, 492, 718, 764
BmrFI CCNGG 3 cut(s) 290, 488, 494
BmsI GCATC 1 cut(s) 575
BpiI GAAGAC 1 cut(s) 457
Bpu1102I GCTNAGC 1 cut(s) 234
Bpu14I TTCGAA 1 cut(s) 452
BpuMI CCSGG 3 cut(s) 290, 488, 494
BsaAI YACGTR 1 cut(s) 781
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 5 cut(s) 48, 223, 486, 492, 612
BsaWI WCCGGW 3 cut(s) 198, 434, 534
BsaXI ACNNNNNCTCC 2 cut(s) 373, 403
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 493, 637, 713
Bse118I RCCGGY 6 cut(s) 68, 203, 229, 506, 637, 872
Bse1I ACTGG 1 cut(s) 895
BseDI CCNNGG 5 cut(s) 48, 223, 486, 492, 612
BseGI GGATG 1 cut(s) 572
BseLI CCNNNNNNNGG 4 cut(s) 229, 493, 637, 713
BseMII CTCAG 3 cut(s) 137, 320, 876
BseNI ACTGG 1 cut(s) 895
BsePI GCGCGC 1 cut(s) 787
BseRI GAGGAG 2 cut(s) 261, 270
BseSI GKGCMC 1 cut(s) 767
BseX3I CGGCCG 1 cut(s) 580
BseXI GCAGC 3 cut(s) 456, 463, 530
BseYI CCCAGC 1 cut(s) 766
Bsh1236I CGCG 3 cut(s) 99, 225, 789
Bsh1285I CGRYCG 2 cut(s) 40, 583
BshFI GGCC 6 cut(s) 47, 207, 491, 497, 582, 699
BshNI GGYRCC 1 cut(s) 762
BsiEI CGRYCG 2 cut(s) 40, 583
BsiHKAI GWGCWC 2 cut(s) 90, 837
BslFI GGGAC 2 cut(s) 278, 851
BslI CCNNNNNNNGG 4 cut(s) 229, 493, 637, 713
BsmAI GTCTC 6 cut(s) 126, 169, 309, 393, 432, 890
BsmBI CGTCTC 3 cut(s) 126, 169, 432
BsmFI GGGAC 2 cut(s) 278, 851
BsnI GGCC 6 cut(s) 47, 207, 491, 497, 582, 699
Bso31I GGTCTC 1 cut(s) 890
Bsp119I TTCGAA 1 cut(s) 452
Bsp1286I GDGCHC 4 cut(s) 90, 719, 767, 837
Bsp143I GATC 4 cut(s) 10, 30, 37, 708
Bsp1720I GCTNAGC 1 cut(s) 234
Bsp19I CCATGG 1 cut(s) 48
BspANI GGCC 6 cut(s) 47, 207, 491, 497, 582, 699
BspCNI CTCAG 3 cut(s) 136, 319, 875
BspFNI CGCG 3 cut(s) 99, 225, 789
BspLI GGNNCC 4 cut(s) 294, 492, 718, 764
BspT104I TTCGAA 1 cut(s) 452
BspT107I GGYRCC 1 cut(s) 762
BspTNI GGTCTC 1 cut(s) 890
BsrBI CCGCTC 1 cut(s) 520
BsrFI RCCGGY 6 cut(s) 68, 203, 229, 506, 637, 872
BsrI ACTGG 1 cut(s) 895
BssAI RCCGGY 6 cut(s) 68, 203, 229, 506, 637, 872
BssECI CCNNGG 5 cut(s) 48, 223, 486, 492, 612
BssHII GCGCGC 1 cut(s) 787
BssMI GATC 4 cut(s) 10, 30, 37, 708
BssSI CACGAG 2 cut(s) 729, 887
BssT1I CCWWGG 2 cut(s) 48, 612
Bst2BI CACGAG 2 cut(s) 729, 887
Bst4CI ACNGT 3 cut(s) 163, 628, 839
Bst6I CTCTTC 1 cut(s) 614
BstBAI YACGTR 1 cut(s) 781
BstBI TTCGAA 1 cut(s) 452
BstC8I GCNNGC 5 cut(s) 214, 558, 639, 755, 789
BstDEI CTNAG 5 cut(s) 123, 234, 306, 597, 862
BstDSI CCRYGG 2 cut(s) 48, 223
BstF5I GGATG 1 cut(s) 572
BstFNI CGCG 3 cut(s) 99, 225, 789
BstHHI GCGC 2 cut(s) 789, 791
BstKTI GATC 4 cut(s) 13, 33, 40, 711
BstMAI GTCTC 6 cut(s) 126, 169, 309, 393, 432, 890
BstMBI GATC 4 cut(s) 10, 30, 37, 708
BstMCI CGRYCG 2 cut(s) 40, 583
BstMWI GCNNNNNNNGC 5 cut(s) 65, 213, 222, 482, 503
BstSCI CCNGG 3 cut(s) 288, 486, 492
BstSLI GKGCMC 1 cut(s) 767
BstUI CGCG 3 cut(s) 99, 225, 789
BstV1I GCAGC 3 cut(s) 456, 463, 530
BstV2I GAAGAC 1 cut(s) 457
BstZI CGGCCG 1 cut(s) 580
BsuRI GGCC 6 cut(s) 47, 207, 491, 497, 582, 699
BtgI CCRYGG 2 cut(s) 48, 223
BtsCI GGATG 1 cut(s) 572
Cac8I GCNNGC 5 cut(s) 214, 558, 639, 755, 789
CfoI GCGC 2 cut(s) 789, 791
Cfr10I RCCGGY 6 cut(s) 68, 203, 229, 506, 637, 872
Cfr13I GGNCC 4 cut(s) 201, 227, 292, 490
Cfr42I CCGCGG 1 cut(s) 226
CpoI CGGWCCG 2 cut(s) 201, 227
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 694
CspI CGGWCCG 2 cut(s) 201, 227
CviAII CATG 5 cut(s) 49, 82, 397, 684, 808
CviQI GTAC 1 cut(s) 694
DdeI CTNAG 5 cut(s) 123, 234, 306, 597, 862
DpnI GATC 4 cut(s) 12, 32, 39, 710
DpnII GATC 4 cut(s) 10, 30, 37, 708
DrdI GACNNNNNNGTC 2 cut(s) 161, 316
DseDI GACNNNNNNGTC 2 cut(s) 161, 316
EaeI YGGCCR 4 cut(s) 45, 205, 580, 697
EagI CGGCCG 1 cut(s) 580
Eam1104I CTCTTC 1 cut(s) 614
EarI CTCTTC 1 cut(s) 614
EciI GGCGGA 3 cut(s) 55, 209, 493
EclXI CGGCCG 1 cut(s) 580
Eco130I CCWWGG 2 cut(s) 48, 612
Eco24I GRGCYC 1 cut(s) 719
Eco31I GGTCTC 1 cut(s) 890
Eco47I GGWCC 3 cut(s) 201, 227, 292
Eco52I CGGCCG 1 cut(s) 580
Eco72I CACGTG 1 cut(s) 781
EcoT14I CCWWGG 2 cut(s) 48, 612
EcoT38I GRGCYC 1 cut(s) 719
ErhI CCWWGG 2 cut(s) 48, 612
Esp3I CGTCTC 3 cut(s) 126, 169, 432
FaeI CATG 5 cut(s) 52, 85, 400, 687, 811
FaiI YATR 7 cut(s) 50, 83, 398, 665, 685, 741, 809
FaqI GGGAC 2 cut(s) 278, 851
FatI CATG 5 cut(s) 48, 81, 396, 683, 807
FauI CCCGC 3 cut(s) 221, 858, 877
Fnu4HI GCNGC 5 cut(s) 60, 223, 445, 477, 544
FokI GGATG 1 cut(s) 559
FriOI GRGCYC 1 cut(s) 719
Fsp4HI GCNGC 5 cut(s) 60, 223, 445, 477, 544
FspBI CTAG 1 cut(s) 243
GlaI GCGC 2 cut(s) 788, 790
GluI GCNGC 5 cut(s) 60, 223, 445, 477, 544
GsaI CCCAGC 1 cut(s) 770
HaeIII GGCC 6 cut(s) 47, 207, 491, 497, 582, 699
HgaI GACGC 1 cut(s) 88
HhaI GCGC 2 cut(s) 789, 791
Hin1II CATG 5 cut(s) 52, 85, 400, 687, 811
Hin6I GCGC 2 cut(s) 787, 789
HinP1I GCGC 2 cut(s) 787, 789
HincII GTYRAC 1 cut(s) 402
HindII GTYRAC 1 cut(s) 402
HinfI GANTC 1 cut(s) 326
HphI GGTGA 8 cut(s) 142, 151, 373, 421, 524, 628, 833, 839
Hpy166II GTNNAC 2 cut(s) 248, 402
Hpy188I TCNGA 4 cut(s) 42, 126, 309, 673
Hpy188III TCNNGA 3 cut(s) 193, 330, 903
Hpy8I GTNNAC 2 cut(s) 248, 402
Hpy99I CGWCG 5 cut(s) 38, 412, 415, 554, 677
HpyAV CCTTC 2 cut(s) 508, 678
HpyCH4III ACNGT 3 cut(s) 163, 628, 839
HpyCH4IV ACGT 2 cut(s) 407, 780
HpyCH4V TGCA 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 213, 222, 482, 503
HpyF3I CTNAG 5 cut(s) 123, 234, 306, 597, 862
HpySE526I ACGT 2 cut(s) 407, 780
Hsp92II CATG 5 cut(s) 52, 85, 400, 687, 811
HspAI GCGC 2 cut(s) 787, 789
KroI GCCGGC 1 cut(s) 637
KroNI GCCGGC 1 cut(s) 639
KspI CCGCGG 1 cut(s) 226
Kzo9I GATC 4 cut(s) 10, 30, 37, 708
LmnI GCTCC 4 cut(s) 85, 274, 722, 784
Lsp1109I GCAGC 3 cut(s) 456, 463, 530
LweI GCATC 1 cut(s) 575
MaeI CTAG 1 cut(s) 243
MaeII ACGT 2 cut(s) 407, 780
MaeIII GTNAC 3 cut(s) 427, 817, 839
MalI GATC 4 cut(s) 12, 32, 39, 710
MbiI CCGCTC 1 cut(s) 520
MboI GATC 4 cut(s) 10, 30, 37, 708
MboII GAAGA 5 cut(s) 65, 414, 457, 615, 631
MfeI CAATTG 1 cut(s) 6
MhlI GDGCHC 4 cut(s) 90, 719, 767, 837
MluCI AATT 2 cut(s) 6, 358
MmeI TCCRAC 2 cut(s) 387, 696
MroNI GCCGGC 1 cut(s) 637
MroXI GAANNNNTTC 1 cut(s) 464
MspA1I CMGCKG 2 cut(s) 225, 476
MspR9I CCNGG 3 cut(s) 290, 488, 494
MunI CAATTG 1 cut(s) 6
MvnI CGCG 3 cut(s) 99, 225, 789
MwoI GCNNNNNNNGC 5 cut(s) 65, 213, 222, 482, 503
NaeI GCCGGC 1 cut(s) 639
NciI CCSGG 3 cut(s) 290, 488, 494
NcoI CCATGG 1 cut(s) 48
NdeII GATC 4 cut(s) 10, 30, 37, 708
NgoMIV GCCGGC 1 cut(s) 637
NlaIII CATG 5 cut(s) 52, 85, 400, 687, 811
NlaIV GGNNCC 4 cut(s) 294, 492, 718, 764
NmeAIII GCCGAG 1 cut(s) 856
NmuCI GTSAC 2 cut(s) 427, 839
NspV TTCGAA 1 cut(s) 452
PauI GCGCGC 1 cut(s) 787
PcsI WCGNNNNNNNCGW 2 cut(s) 822, 885
PdiI GCCGGC 1 cut(s) 639
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 5 cut(s) 61, 224, 446, 478, 545
Ple19I CGATCG 1 cut(s) 40
PmaCI CACGTG 1 cut(s) 781
PmlI CACGTG 1 cut(s) 781
Ppu21I YACGTR 1 cut(s) 781
PsiI TTATAA 1 cut(s) 741
PspCI CACGTG 1 cut(s) 781
PspFI CCCAGC 1 cut(s) 766
PspN4I GGNNCC 4 cut(s) 294, 492, 718, 764
PspPI GGNCC 4 cut(s) 201, 227, 292, 490
PteI GCGCGC 1 cut(s) 787
PvuI CGATCG 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 1 cut(s) 695
RsaNI GTAC 1 cut(s) 694
Rsr2I CGGWCCG 2 cut(s) 201, 227
RsrII CGGWCCG 2 cut(s) 201, 227
SacII CCGCGG 1 cut(s) 226
SatI GCNGC 5 cut(s) 60, 223, 445, 477, 544
Sau3AI GATC 4 cut(s) 10, 30, 37, 708
Sau96I GGNCC 4 cut(s) 201, 227, 292, 490
ScrFI CCNGG 3 cut(s) 290, 488, 494
SduI GDGCHC 4 cut(s) 90, 719, 767, 837
SfaNI GCATC 1 cut(s) 575
Sfr303I CCGCGG 1 cut(s) 226
SfuI TTCGAA 1 cut(s) 452
SgrAI CRCCGGYG 1 cut(s) 872
SgrBI CCGCGG 1 cut(s) 226
SinI GGWCC 3 cut(s) 201, 227, 292
Sse9I AATT 2 cut(s) 6, 358
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 3 cut(s) 288, 486, 492
StyI CCWWGG 2 cut(s) 48, 612
TaaI ACNGT 3 cut(s) 163, 628, 839
TaiI ACGT 2 cut(s) 410, 783
TaqI TCGA 3 cut(s) 33, 135, 452
TasI AATT 2 cut(s) 6, 358
TauI GCSGC 2 cut(s) 62, 225
TfiI GAWTC 1 cut(s) 326
TseFI GTSAC 2 cut(s) 427, 839
TseI GCWGC 3 cut(s) 444, 476, 543
Tsp45I GTSAC 2 cut(s) 427, 839
TspDTI ATGAA 2 cut(s) 172, 672
TspGWI ACGGA 3 cut(s) 178, 415, 620
VpaK11BI GGWCC 3 cut(s) 201, 227, 292
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.