pycom16g20580
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002334
Physical Location & Seq
Reverse (-)
18329337 .. 18330438
1102 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g20580.1

Sequence Viewer

Length: 843 bp
ATGGCTGTAGATCTAGTTGGCTTCTCCAAGATTGATGATCGGGCGGCCATGCAAGAAGCTGCCTCCGCTGGCTTGCAAAGCATGGAGCACCTCATCCGCGCCCTATCCAATCACCCCCCGTCCCAAACCCCACTCGATTGCCGGGAAATCACCGACTTCACCGTGACAAAGTTCAAGCAGCTAATCTCTGTTCTGAACCGGACCGGTCACGCGCGGTTCCGCCGTGGACCGGCCAATCCACCTTCCGACCCGGTCCACCCAAAACCTCAGACGACTTTGACTGTTTTTCAAACACCAAAGTCCGATAAAGATTCCTCCACTACTCTGTCCCCGCCGCTATCCACTACGTCGTCGTTTCTATCCTCCATCACAATCGGTGACGGCAGCGTTTCCAACGGTAAAGCCTTCTCATCGATTTCCGTGCCTCCGGCGCCGGCTTTCTCCGCTGGAAAACCCCCCCTTCCACAATCTCACCGGAAACGGTGTCACGACGGCGAAACAGCTAAAACATCATCGTCTGGCCACTGCCATTGCTCTAAAAGAAGGAAGTCTAAGGTTAAGAGGACGATCAGAGTGCCGGCGGTTAGTTCGAAAATCGCCGATATACCGGCCGATGAGTTCACCTGGAGAAAGTACGGTCAAAAGCCGATCAAGGGCTCGCCTTACCCGAGAGGGTATTATAAGTGCAGTACAGTGAGAGGCTGTCCGGCGAGGAAACACGTGGAGAGAGCTCAGGACGACCCCACTATGCTCGTCGTTACCTACGAGGCTGAGCACCATCACCCGCACCCTTCCCTCACAGCCGCAAATGTGGGCCTTGTTTTCCAATCTTCCAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plant_zn_clust WRKY

Protein Analysis

281

Amino Acids

30.49

Weight (kDa)

9.78

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_zn_clust PF10533 159 - 201 4.6e-17 Plant zinc cluster domain
WRKY PF03106 205 - 262 6.1e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 681
AccB1I GGYRCC 1 cut(s) 430
AccII CGCG 3 cut(s) 99, 212, 214
AcoI YGGCCR 4 cut(s) 45, 231, 520, 609
AcvI CACGTG 1 cut(s) 721
AcyI GRCGYC 1 cut(s) 431
AfaI GTAC 2 cut(s) 635, 691
AfiI CCNNNNNNNGG 2 cut(s) 229, 653
AflIII ACRYGT 1 cut(s) 718
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 2 cut(s) 175, 290
AjnI CCWGG 1 cut(s) 623
AluBI AGCT 4 cut(s) 59, 181, 503, 731
AluI AGCT 4 cut(s) 59, 181, 503, 731
Alw21I GWGCWC 3 cut(s) 90, 733, 777
Ama87I CYCGRG 1 cut(s) 667
AoxI GGCC 5 cut(s) 45, 231, 520, 609, 814
ApeKI GCWGC 3 cut(s) 59, 178, 384
AsiGI ACCGGT 1 cut(s) 203
AspLEI GCGC 3 cut(s) 101, 214, 433
AspS9I GGNCC 4 cut(s) 201, 227, 253, 814
AsuC2I CCSGG 2 cut(s) 143, 251
AsuHPI GGTGA 7 cut(s) 104, 142, 151, 389, 464, 613, 773
AsuII TTCGAA 1 cut(s) 590
AvaI CYCGRG 1 cut(s) 667
AvaII GGWCC 3 cut(s) 201, 227, 253
BalI TGGCCA 1 cut(s) 522
BanI GGYRCC 1 cut(s) 430
BanII GRGCYC 2 cut(s) 659, 733
BbrPI CACGTG 1 cut(s) 721
Bbv12I GWGCWC 3 cut(s) 90, 733, 777
BbvI GCAGC 3 cut(s) 46, 190, 396
BccI CCATC 2 cut(s) 374, 786
BceAI ACGGC 3 cut(s) 207, 397, 508
BcgI CGANNNNNNTGC 4 cut(s) 403, 437, 556, 590
BciT130I CCWGG 1 cut(s) 625
BcnI CCSGG 2 cut(s) 143, 251
BfaI CTAG 1 cut(s) 14
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 434
BglII AGATCT 1 cut(s) 10
BisI GCNGC 6 cut(s) 45, 60, 179, 335, 385, 804
BlpI GCTNAGC 1 cut(s) 771
BlsI GCNGC 6 cut(s) 46, 61, 180, 336, 386, 805
Bme1390I CCNGG 3 cut(s) 143, 251, 625
Bme18I GGWCC 3 cut(s) 201, 227, 253
BmeT110I CYCGRG 1 cut(s) 667
BmgT120I GGNCC 4 cut(s) 201, 227, 253, 814
BmiI GGNNCC 2 cut(s) 218, 432
BmrFI CCNGG 3 cut(s) 143, 251, 625
BpmI CTGGAG 1 cut(s) 646
Bpu10I CCTNAGC 1 cut(s) 732
Bpu1102I GCTNAGC 1 cut(s) 771
Bpu14I TTCGAA 1 cut(s) 590
BpuMI CCSGG 2 cut(s) 143, 251
Bsa29I ATCGAT 1 cut(s) 413
BsaAI YACGTR 1 cut(s) 721
BsaHI GRCGYC 1 cut(s) 431
BsaJI CCNNGG 2 cut(s) 223, 834
BsaWI WCCGGW 3 cut(s) 198, 203, 474
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 653
Bse118I RCCGGY 5 cut(s) 203, 229, 433, 577, 607
Bse3DI GCAATG 1 cut(s) 529
BseBI CCWGG 1 cut(s) 625
BseCI ATCGAT 1 cut(s) 413
BseDI CCNNGG 2 cut(s) 223, 834
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 2 cut(s) 229, 653
BseMI GCAATG 1 cut(s) 529
BseMII CTCAG 3 cut(s) 281, 746, 762
BseX3I CGGCCG 1 cut(s) 609
BseXI GCAGC 3 cut(s) 46, 190, 396
BsgI GTGCAG 1 cut(s) 706
Bsh1236I CGCG 3 cut(s) 99, 212, 214
Bsh1285I CGRYCG 1 cut(s) 612
BshFI GGCC 5 cut(s) 47, 233, 522, 611, 816
BshNI GGYRCC 1 cut(s) 430
BshTI ACCGGT 1 cut(s) 203
BshVI ATCGAT 1 cut(s) 413
BsiEI CGRYCG 1 cut(s) 612
BsiHKAI GWGCWC 3 cut(s) 90, 733, 777
BsiHKCI CYCGRG 1 cut(s) 667
BslFI GGGAC 2 cut(s) 106, 313
BslI CCNNNNNNNGG 2 cut(s) 229, 653
BsmFI GGGAC 2 cut(s) 106, 313
BsnI GGCC 5 cut(s) 47, 233, 522, 611, 816
BsoBI CYCGRG 1 cut(s) 667
Bsp119I TTCGAA 1 cut(s) 590
Bsp1286I GDGCHC 4 cut(s) 90, 659, 733, 777
Bsp143I GATC 4 cut(s) 10, 37, 567, 648
Bsp1720I GCTNAGC 1 cut(s) 771
BspANI GGCC 5 cut(s) 47, 233, 522, 611, 816
BspCNI CTCAG 3 cut(s) 280, 745, 763
BspDI ATCGAT 1 cut(s) 413
BspFNI CGCG 3 cut(s) 99, 212, 214
BspLI GGNNCC 2 cut(s) 218, 432
BspT104I TTCGAA 1 cut(s) 590
BspT107I GGYRCC 1 cut(s) 430
BsrDI GCAATG 1 cut(s) 529
BsrFI RCCGGY 5 cut(s) 203, 229, 433, 577, 607
BssAI RCCGGY 5 cut(s) 203, 229, 433, 577, 607
BssECI CCNNGG 2 cut(s) 223, 834
BssMI GATC 4 cut(s) 10, 37, 567, 648
BssNI GRCGYC 1 cut(s) 431
BssT1I CCWWGG 1 cut(s) 834
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 6 cut(s) 163, 283, 398, 483, 638, 694
BstACI GRCGYC 1 cut(s) 431
BstBAI YACGTR 1 cut(s) 721
BstBI TTCGAA 1 cut(s) 590
BstC8I GCNNGC 5 cut(s) 70, 74, 435, 579, 659
BstDEI CTNAG 4 cut(s) 267, 552, 732, 771
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 3 cut(s) 99, 212, 214
BstH2I RGCGCY 1 cut(s) 434
BstHHI GCGC 3 cut(s) 101, 214, 433
BstKTI GATC 4 cut(s) 13, 40, 570, 651
BstMBI GATC 4 cut(s) 10, 37, 567, 648
BstMCI CGRYCG 1 cut(s) 612
BstMWI GCNNNNNNNGC 4 cut(s) 65, 78, 430, 443
BstNI CCWGG 1 cut(s) 625
BstSCI CCNGG 3 cut(s) 141, 249, 623
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 3 cut(s) 99, 212, 214
BstV1I GCAGC 3 cut(s) 46, 190, 396
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
BstZI CGGCCG 1 cut(s) 609
Bsu15I ATCGAT 1 cut(s) 413
BsuRI GGCC 5 cut(s) 47, 233, 522, 611, 816
BsuTUI ATCGAT 1 cut(s) 413
BtgI CCRYGG 1 cut(s) 223
BtsCI GGATG 1 cut(s) 93
BtsI GCAGTG 1 cut(s) 523
BtsIMutI CAGTG 2 cut(s) 523, 699
Cac8I GCNNGC 5 cut(s) 70, 74, 435, 579, 659
CfoI GCGC 3 cut(s) 101, 214, 433
Cfr10I RCCGGY 5 cut(s) 203, 229, 433, 577, 607
Cfr13I GGNCC 4 cut(s) 201, 227, 253, 814
ClaI ATCGAT 1 cut(s) 413
CpoI CGGWCCG 1 cut(s) 201
Csp6I GTAC 2 cut(s) 634, 690
CspAI ACCGGT 1 cut(s) 203
CspCI CAANNNNNGTGG 2 cut(s) 512, 547
CspI CGGWCCG 1 cut(s) 201
CviAII CATG 2 cut(s) 49, 82
CviQI GTAC 2 cut(s) 634, 690
DdeI CTNAG 4 cut(s) 267, 552, 732, 771
DinI GGCGCC 1 cut(s) 432
DpnI GATC 4 cut(s) 12, 39, 569, 650
DpnII GATC 4 cut(s) 10, 37, 567, 648
EaeI YGGCCR 4 cut(s) 45, 231, 520, 609
EagI CGGCCG 1 cut(s) 609
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 1 cut(s) 731
EclXI CGGCCG 1 cut(s) 609
Eco130I CCWWGG 1 cut(s) 834
Eco24I GRGCYC 2 cut(s) 659, 733
Eco47I GGWCC 3 cut(s) 201, 227, 253
Eco52I CGGCCG 1 cut(s) 609
Eco53kI GAGCTC 1 cut(s) 731
Eco72I CACGTG 1 cut(s) 721
Eco88I CYCGRG 1 cut(s) 667
EcoICRI GAGCTC 1 cut(s) 731
EcoRII CCWGG 1 cut(s) 623
EcoT14I CCWWGG 1 cut(s) 834
EcoT38I GRGCYC 2 cut(s) 659, 733
EgeI GGCGCC 1 cut(s) 432
EheI GGCGCC 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 834
FaeI CATG 2 cut(s) 52, 85
FaiI YATR 5 cut(s) 50, 83, 605, 681, 749
FaqI GGGAC 2 cut(s) 106, 313
FatI CATG 2 cut(s) 48, 81
FauI CCCGC 2 cut(s) 339, 792
Fnu4HI GCNGC 6 cut(s) 45, 60, 179, 335, 385, 804
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 2 cut(s) 659, 733
Fsp4HI GCNGC 6 cut(s) 45, 60, 179, 335, 385, 804
FspBI CTAG 1 cut(s) 14
GlaI GCGC 3 cut(s) 100, 213, 432
GluI GCNGC 6 cut(s) 45, 60, 179, 335, 385, 804
GsuI CTGGAG 1 cut(s) 646
HaeII RGCGCY 1 cut(s) 434
HaeIII GGCC 5 cut(s) 47, 233, 522, 611, 816
HhaI GCGC 3 cut(s) 101, 214, 433
Hin1I GRCGYC 1 cut(s) 431
Hin1II CATG 2 cut(s) 52, 85
Hin6I GCGC 3 cut(s) 99, 212, 431
HinP1I GCGC 3 cut(s) 99, 212, 431
HinfI GANTC 1 cut(s) 311
HphI GGTGA 7 cut(s) 104, 142, 151, 389, 464, 613, 773
Hpy166II GTNNAC 3 cut(s) 227, 256, 621
Hpy188I TCNGA 5 cut(s) 195, 247, 270, 304, 572
Hpy188III TCNNGA 2 cut(s) 488, 734
Hpy8I GTNNAC 3 cut(s) 227, 256, 621
Hpy99I CGWCG 4 cut(s) 352, 355, 494, 758
HpyAV CCTTC 5 cut(s) 252, 415, 470, 537, 801
HpyCH4III ACNGT 6 cut(s) 163, 283, 398, 483, 638, 694
HpyCH4IV ACGT 2 cut(s) 347, 720
HpyCH4V TGCA 3 cut(s) 52, 76, 687
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 78, 430, 443
HpyF3I CTNAG 4 cut(s) 267, 552, 732, 771
HpySE526I ACGT 2 cut(s) 347, 720
Hsp92I GRCGYC 1 cut(s) 431
Hsp92II CATG 2 cut(s) 52, 85
HspAI GCGC 3 cut(s) 99, 212, 431
KasI GGCGCC 1 cut(s) 430
KroI GCCGGC 2 cut(s) 433, 577
KroNI GCCGGC 2 cut(s) 435, 579
Kzo9I GATC 4 cut(s) 10, 37, 567, 648
LmnI GCTCC 1 cut(s) 85
Lsp1109I GCAGC 3 cut(s) 46, 190, 396
MaeI CTAG 1 cut(s) 14
MaeII ACGT 2 cut(s) 347, 720
MaeIII GTNAC 5 cut(s) 163, 206, 377, 485, 757
MalI GATC 4 cut(s) 12, 39, 569, 650
MboI GATC 4 cut(s) 10, 37, 567, 648
MboII GAAGA 1 cut(s) 822
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 4 cut(s) 90, 659, 733, 777
MlsI TGGCCA 1 cut(s) 522
MluNI TGGCCA 1 cut(s) 522
Mly113I GGCGCC 1 cut(s) 431
MmeI TCCRAC 2 cut(s) 270, 417
Mox20I TGGCCA 1 cut(s) 522
MroNI GCCGGC 2 cut(s) 433, 577
MscI TGGCCA 1 cut(s) 522
MseI TTAA 1 cut(s) 558
Msp20I TGGCCA 1 cut(s) 522
MspA1I CMGCKG 2 cut(s) 68, 446
MspR9I CCNGG 3 cut(s) 143, 251, 625
MvaI CCWGG 1 cut(s) 625
MvnI CGCG 3 cut(s) 99, 212, 214
MwoI GCNNNNNNNGC 4 cut(s) 65, 78, 430, 443
NaeI GCCGGC 2 cut(s) 435, 579
NarI GGCGCC 1 cut(s) 431
NciI CCSGG 2 cut(s) 143, 251
NdeII GATC 4 cut(s) 10, 37, 567, 648
NgoMIV GCCGGC 2 cut(s) 433, 577
NlaIII CATG 2 cut(s) 52, 85
NlaIV GGNNCC 2 cut(s) 218, 432
NmuCI GTSAC 4 cut(s) 163, 206, 377, 485
NspV TTCGAA 1 cut(s) 590
PcsI WCGNNNNNNNCGW 2 cut(s) 665, 762
PdiI GCCGGC 2 cut(s) 435, 579
PfeI GAWTC 1 cut(s) 311
PflFI GACNNNGTC 1 cut(s) 251
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 6 cut(s) 46, 61, 180, 336, 386, 805
PluTI GGCGCC 1 cut(s) 434
PmaCI CACGTG 1 cut(s) 721
PmlI CACGTG 1 cut(s) 721
Ppu21I YACGTR 1 cut(s) 721
PsiI TTATAA 1 cut(s) 681
Psp124BI GAGCTC 1 cut(s) 733
Psp6I CCWGG 1 cut(s) 623
PspCI CACGTG 1 cut(s) 721
PspGI CCWGG 1 cut(s) 623
PspN4I GGNNCC 2 cut(s) 218, 432
PspPI GGNCC 4 cut(s) 201, 227, 253, 814
PsuI RGATCY 1 cut(s) 10
PsyI GACNNNGTC 1 cut(s) 251
RsaI GTAC 2 cut(s) 635, 691
RsaNI GTAC 2 cut(s) 634, 690
Rsr2I CGGWCCG 1 cut(s) 201
RsrII CGGWCCG 1 cut(s) 201
SacI GAGCTC 1 cut(s) 733
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 6 cut(s) 45, 60, 179, 335, 385, 804
Sau3AI GATC 4 cut(s) 10, 37, 567, 648
Sau96I GGNCC 4 cut(s) 201, 227, 253, 814
ScrFI CCNGG 3 cut(s) 143, 251, 625
SduI GDGCHC 4 cut(s) 90, 659, 733, 777
SfcI CTRYAG 1 cut(s) 6
SfoI GGCGCC 1 cut(s) 432
SfuI TTCGAA 1 cut(s) 590
SinI GGWCC 3 cut(s) 201, 227, 253
SspDI GGCGCC 1 cut(s) 430
SspMI CTAG 1 cut(s) 14
SstI GAGCTC 1 cut(s) 733
StyD4I CCNGG 3 cut(s) 141, 249, 623
StyI CCWWGG 1 cut(s) 834
TaaI ACNGT 6 cut(s) 163, 283, 398, 483, 638, 694
TaiI ACGT 2 cut(s) 350, 723
TaqI TCGA 3 cut(s) 135, 413, 590
TatI WGTACW 1 cut(s) 689
TauI GCSGC 3 cut(s) 47, 337, 806
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TscAI CASTG 2 cut(s) 530, 699
TseFI GTSAC 4 cut(s) 163, 206, 377, 485
TseI GCWGC 3 cut(s) 59, 178, 384
Tsp45I GTSAC 4 cut(s) 163, 206, 377, 485
TspGWI ACGGA 1 cut(s) 409
TspRI CASTG 2 cut(s) 530, 699
Tth111I GACNNNGTC 1 cut(s) 251
VpaK11BI GGWCC 3 cut(s) 201, 227, 253
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.