RLG00000009325
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002334
Physical Location & Seq
Forward (+)
51073733 .. 51074880
1148 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009325

Sequence Viewer

Length: 927 bp
ATGGCAATCGATCTCGTTAGGTTCTCCAAGATCGACGATCGGACGGCCATGGAAGAGGCCGCCTCCGCCGGCTTACAGAGCATGGAGCATCTCATCCGTGTCTTGTCCACTCAAACCCCGTCTCACAACAACCTCGACTGCCGTGAAATCACCGACTTCACCGTCGCCAAGTTCAAGCAGTTCATCTCCGTCTTGAACCGGACCGGTCACGCCCGCTTCCGCCGCGGACCGGTCAAGCAACCTAGTGAATCTACCACCACCTATTCTCCTCCTCCTCCTCCACCTCCACCTCCTGCCAACCCGGTCCCACCAAAGCCTCAGGTTACTTTGTCTTTTGAAACCGGAAATCCTAATACCAATACCAATACAAACACCAATTCCAAGTCCAAAGACTGCCTCACCTCATCTCCTCCCATGTCCACAACATCGTCGTTTCTTTCTTCCGTGACCACCGGAGACGGCAGCGTTTCCAACGGCAAAGTTTTCTCTTCAGCTGCCGTACCCCCGGCGCCGGCCTTCTCCACCGGCAAACCCCCGCTCTCCCAATCCCACCGGAAACGCTGCCACGACGAGCTCGCAACCAAAACGACATCGTCCGGTCGATGCCATTGTTCTAAAAGAAGAAAATCCAAGGTGAAGAGGACAGTCCGAGTGCCGGCGATTAGTTCGAAAATCGCCGATATACCTTCCGACGAGTTTTCGTGGAGGAAGTACGGCCAAAAGCCGATCAAGGGCTCCCCATACCCACGAGGCTATTATAAGTGTAGTAGCGTTCGAGGGTGTCCAGCAAGAAAGCACGTGGAGCGCGCCCAAGACGACCCCACCATGCTTATCGTTACCTACGAAGCGGAGCACCGTCACCCTCACCCGCAGCAGTCAAACTCGGTCCCGGCTGGTGTTGTCGGACTCGTGTTCCAATCCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plant_zn_clust WRKY

Protein Analysis

309

Amino Acids

33.58

Weight (kDa)

9.88

Isoelectric Point (pI)

50.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_zn_clust PF10533 185 - 227 1.9e-17 Plant zinc cluster domain
WRKY PF03106 231 - 288 3.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
AasI GACNNNNNNGTC 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 508
AccBSI CCGCTC 1 cut(s) 538
AccII CGCG 2 cut(s) 225, 807
AcoI YGGCCR 2 cut(s) 45, 715
AcuI CTGAAG 1 cut(s) 474
AcvI CACGTG 1 cut(s) 799
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 2 cut(s) 501, 713
AfiI CCNNNNNNNGG 4 cut(s) 229, 511, 655, 731
AgeI ACCGGT 2 cut(s) 203, 229
AgsI TTSAA 3 cut(s) 175, 196, 338
AluBI AGCT 2 cut(s) 494, 574
AluI AGCT 2 cut(s) 494, 574
Alw21I GWGCWC 2 cut(s) 576, 855
Alw26I GTCTC 2 cut(s) 126, 450
AoxI GGCC 4 cut(s) 45, 57, 513, 715
ApeKI GCWGC 4 cut(s) 462, 494, 561, 871
AsiGI ACCGGT 2 cut(s) 203, 229
AspLEI GCGC 3 cut(s) 511, 807, 809
AspS9I GGNCC 4 cut(s) 201, 227, 304, 886
AsuC2I CCSGG 3 cut(s) 302, 506, 890
AsuHPI GGTGA 6 cut(s) 142, 151, 391, 646, 851, 857
AsuII TTCGAA 1 cut(s) 668
AvaII GGWCC 4 cut(s) 201, 227, 304, 886
AxyI CCTNAGG 1 cut(s) 318
BanI GGYRCC 1 cut(s) 508
BanII GRGCYC 2 cut(s) 576, 737
BauI CACGAG 2 cut(s) 747, 908
BbrPI CACGTG 1 cut(s) 799
Bbv12I GWGCWC 2 cut(s) 576, 855
BbvI GCAGC 4 cut(s) 474, 481, 548, 883
BceAI ACGGC 6 cut(s) 60, 126, 475, 482, 490, 730
BcnI CCSGG 3 cut(s) 302, 506, 890
BcoDI GTCTC 2 cut(s) 126, 450
BfaI CTAG 1 cut(s) 243
BfoI RGCGCY 1 cut(s) 512
BisI GCNGC 6 cut(s) 60, 223, 463, 495, 562, 872
BlsI GCNGC 6 cut(s) 61, 224, 464, 496, 563, 873
Bme1390I CCNGG 3 cut(s) 302, 506, 890
Bme18I GGWCC 4 cut(s) 201, 227, 304, 886
BmgT120I GGNCC 4 cut(s) 201, 227, 304, 886
BmiI GGNNCC 4 cut(s) 306, 510, 736, 888
BmrFI CCNGG 3 cut(s) 302, 506, 890
BmsI GCATC 2 cut(s) 97, 593
BplI GAGNNNNNCTC 2 cut(s) 47, 79
Bpu14I TTCGAA 1 cut(s) 668
BpuMI CCSGG 3 cut(s) 302, 506, 890
Bsa29I ATCGAT 1 cut(s) 9
BsaAI YACGTR 1 cut(s) 799
BsaHI GRCGYC 1 cut(s) 509
BsaJI CCNNGG 4 cut(s) 48, 223, 504, 630
BsaWI WCCGGW 7 cut(s) 198, 203, 229, 341, 452, 552, 596
BsaXI ACNNNNNCTCC 4 cut(s) 250, 280, 391, 421
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 511, 655, 731
Bse118I RCCGGY 6 cut(s) 68, 203, 229, 511, 524, 655
Bse21I CCTNAGG 1 cut(s) 318
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 4 cut(s) 48, 223, 504, 630
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 4 cut(s) 229, 511, 655, 731
BseMII CTCAG 1 cut(s) 332
BsePI GCGCGC 1 cut(s) 805
BseRI GAGGAG 5 cut(s) 258, 261, 264, 267, 399
BseXI GCAGC 4 cut(s) 474, 481, 548, 883
Bsh1236I CGCG 2 cut(s) 225, 807
Bsh1285I CGRYCG 2 cut(s) 40, 601
BshFI GGCC 4 cut(s) 47, 59, 515, 717
BshNI GGYRCC 1 cut(s) 508
BshTI ACCGGT 2 cut(s) 203, 229
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 2 cut(s) 40, 601
BsiHKAI GWGCWC 2 cut(s) 576, 855
BslFI GGGAC 2 cut(s) 290, 872
BslI CCNNNNNNNGG 4 cut(s) 229, 511, 655, 731
BsmAI GTCTC 2 cut(s) 126, 450
BsmBI CGTCTC 2 cut(s) 126, 450
BsmFI GGGAC 2 cut(s) 290, 872
BsnI GGCC 4 cut(s) 47, 59, 515, 717
Bsp119I TTCGAA 1 cut(s) 668
Bsp1286I GDGCHC 3 cut(s) 576, 737, 855
Bsp143I GATC 4 cut(s) 10, 30, 37, 726
Bsp19I CCATGG 1 cut(s) 48
BspANI GGCC 4 cut(s) 47, 59, 515, 717
BspCNI CTCAG 1 cut(s) 331
BspDI ATCGAT 1 cut(s) 9
BspFNI CGCG 2 cut(s) 225, 807
BspLI GGNNCC 4 cut(s) 306, 510, 736, 888
BspT104I TTCGAA 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 508
BsrBI CCGCTC 1 cut(s) 538
BsrFI RCCGGY 6 cut(s) 68, 203, 229, 511, 524, 655
BssAI RCCGGY 6 cut(s) 68, 203, 229, 511, 524, 655
BssECI CCNNGG 4 cut(s) 48, 223, 504, 630
BssHII GCGCGC 1 cut(s) 805
BssMI GATC 4 cut(s) 10, 30, 37, 726
BssNI GRCGYC 1 cut(s) 509
BssSI CACGAG 2 cut(s) 747, 908
BssT1I CCWWGG 2 cut(s) 48, 630
Bst2BI CACGAG 2 cut(s) 747, 908
Bst4CI ACNGT 3 cut(s) 163, 646, 857
Bst6I CTCTTC 3 cut(s) 48, 493, 632
BstACI GRCGYC 1 cut(s) 509
BstBAI YACGTR 1 cut(s) 799
BstBI TTCGAA 1 cut(s) 668
BstC8I GCNNGC 6 cut(s) 70, 214, 513, 576, 657, 807
BstDEI CTNAG 1 cut(s) 318
BstDSI CCRYGG 2 cut(s) 48, 223
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 2 cut(s) 225, 807
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 3 cut(s) 511, 807, 809
BstKTI GATC 4 cut(s) 13, 33, 40, 729
BstMAI GTCTC 2 cut(s) 126, 450
BstMBI GATC 4 cut(s) 10, 30, 37, 726
BstMCI CGRYCG 2 cut(s) 40, 601
BstMWI GCNNNNNNNGC 4 cut(s) 65, 78, 222, 802
BstSCI CCNGG 3 cut(s) 300, 504, 888
BstUI CGCG 2 cut(s) 225, 807
BstV1I GCAGC 4 cut(s) 474, 481, 548, 883
Bsu15I ATCGAT 1 cut(s) 9
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 4 cut(s) 47, 59, 515, 717
BsuTUI ATCGAT 1 cut(s) 9
BtgI CCRYGG 2 cut(s) 48, 223
BtsCI GGATG 1 cut(s) 93
Cac8I GCNNGC 6 cut(s) 70, 214, 513, 576, 657, 807
CfoI GCGC 3 cut(s) 511, 807, 809
Cfr10I RCCGGY 6 cut(s) 68, 203, 229, 511, 524, 655
Cfr13I GGNCC 4 cut(s) 201, 227, 304, 886
Cfr42I CCGCGG 1 cut(s) 226
ClaI ATCGAT 1 cut(s) 9
CpoI CGGWCCG 2 cut(s) 201, 227
Csp6I GTAC 2 cut(s) 500, 712
CspAI ACCGGT 2 cut(s) 203, 229
CspI CGGWCCG 2 cut(s) 201, 227
CviAII CATG 4 cut(s) 49, 82, 415, 826
CviQI GTAC 2 cut(s) 500, 712
DdeI CTNAG 1 cut(s) 318
DinI GGCGCC 1 cut(s) 510
DpnI GATC 4 cut(s) 12, 32, 39, 728
DpnII GATC 4 cut(s) 10, 30, 37, 726
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 2 cut(s) 45, 715
Eam1104I CTCTTC 3 cut(s) 48, 493, 632
EarI CTCTTC 3 cut(s) 48, 493, 632
EciI GGCGGA 3 cut(s) 55, 209, 910
Ecl136II GAGCTC 1 cut(s) 574
Eco130I CCWWGG 2 cut(s) 48, 630
Eco24I GRGCYC 2 cut(s) 576, 737
Eco47I GGWCC 4 cut(s) 201, 227, 304, 886
Eco53kI GAGCTC 1 cut(s) 574
Eco57I CTGAAG 1 cut(s) 474
Eco72I CACGTG 1 cut(s) 799
Eco81I CCTNAGG 1 cut(s) 318
EcoICRI GAGCTC 1 cut(s) 574
EcoT14I CCWWGG 2 cut(s) 48, 630
EcoT38I GRGCYC 2 cut(s) 576, 737
EgeI GGCGCC 1 cut(s) 510
EheI GGCGCC 1 cut(s) 510
ErhI CCWWGG 2 cut(s) 48, 630
Esp3I CGTCTC 2 cut(s) 126, 450
FaeI CATG 4 cut(s) 52, 85, 418, 829
FaiI YATR 7 cut(s) 50, 83, 416, 683, 742, 759, 827
FaqI GGGAC 2 cut(s) 290, 872
FatI CATG 4 cut(s) 48, 81, 414, 825
FauI CCCGC 3 cut(s) 221, 543, 876
Fnu4HI GCNGC 6 cut(s) 60, 223, 463, 495, 562, 872
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 2 cut(s) 576, 737
Fsp4HI GCNGC 6 cut(s) 60, 223, 463, 495, 562, 872
FspBI CTAG 1 cut(s) 243
GlaI GCGC 3 cut(s) 510, 806, 808
GluI GCNGC 6 cut(s) 60, 223, 463, 495, 562, 872
HaeII RGCGCY 1 cut(s) 512
HaeIII GGCC 4 cut(s) 47, 59, 515, 717
HhaI GCGC 3 cut(s) 511, 807, 809
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 4 cut(s) 52, 85, 418, 829
Hin6I GCGC 3 cut(s) 509, 805, 807
HinP1I GCGC 3 cut(s) 509, 805, 807
HinfI GANTC 2 cut(s) 248, 906
HphI GGTGA 6 cut(s) 142, 151, 391, 646, 851, 857
Hpy166II GTNNAC 2 cut(s) 108, 420
Hpy188I TCNGA 4 cut(s) 42, 650, 691, 905
Hpy188III TCNNGA 1 cut(s) 193
Hpy8I GTNNAC 2 cut(s) 108, 420
Hpy99I CGWCG 5 cut(s) 38, 167, 433, 572, 695
HpyAV CCTTC 2 cut(s) 526, 696
HpyCH4III ACNGT 3 cut(s) 163, 646, 857
HpyCH4IV ACGT 1 cut(s) 798
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 78, 222, 802
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 798
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 4 cut(s) 52, 85, 418, 829
HspAI GCGC 3 cut(s) 509, 805, 807
KasI GGCGCC 1 cut(s) 508
KroI GCCGGC 3 cut(s) 68, 511, 655
KroNI GCCGGC 3 cut(s) 70, 513, 657
KspI CCGCGG 1 cut(s) 226
Kzo9I GATC 4 cut(s) 10, 30, 37, 726
LmnI GCTCC 4 cut(s) 85, 740, 802, 850
Lsp1109I GCAGC 4 cut(s) 474, 481, 548, 883
LweI GCATC 2 cut(s) 97, 593
MaeI CTAG 1 cut(s) 243
MaeII ACGT 1 cut(s) 798
MaeIII GTNAC 5 cut(s) 206, 322, 445, 835, 857
MalI GATC 4 cut(s) 12, 32, 39, 728
MbiI CCGCTC 1 cut(s) 538
MboI GATC 4 cut(s) 10, 30, 37, 726
MboII GAAGA 5 cut(s) 65, 432, 480, 633, 649
MhlI GDGCHC 3 cut(s) 576, 737, 855
MluCI AATT 1 cut(s) 376
Mly113I GGCGCC 1 cut(s) 509
MlyI GAGTC 1 cut(s) 900
MmeI TCCRAC 3 cut(s) 495, 714, 883
MroNI GCCGGC 3 cut(s) 68, 511, 655
MspA1I CMGCKG 2 cut(s) 225, 494
MspR9I CCNGG 3 cut(s) 302, 506, 890
MvnI CGCG 2 cut(s) 225, 807
MwoI GCNNNNNNNGC 4 cut(s) 65, 78, 222, 802
NaeI GCCGGC 3 cut(s) 70, 513, 657
NarI GGCGCC 1 cut(s) 509
NciI CCSGG 3 cut(s) 302, 506, 890
NcoI CCATGG 1 cut(s) 48
NdeII GATC 4 cut(s) 10, 30, 37, 726
NgoMIV GCCGGC 3 cut(s) 68, 511, 655
NlaIII CATG 4 cut(s) 52, 85, 418, 829
NlaIV GGNNCC 4 cut(s) 306, 510, 736, 888
NmuCI GTSAC 3 cut(s) 206, 445, 857
NspV TTCGAA 1 cut(s) 668
PauI GCGCGC 1 cut(s) 805
PcsI WCGNNNNNNNCGW 1 cut(s) 840
PdiI GCCGGC 3 cut(s) 70, 513, 657
PfeI GAWTC 1 cut(s) 248
PflFI GACNNNGTC 1 cut(s) 592
PinAI ACCGGT 2 cut(s) 203, 229
PkrI GCNGC 6 cut(s) 61, 224, 464, 496, 563, 873
Ple19I CGATCG 1 cut(s) 40
PleI GAGTC 1 cut(s) 900
PluTI GGCGCC 1 cut(s) 512
PmaCI CACGTG 1 cut(s) 799
PmlI CACGTG 1 cut(s) 799
PpsI GAGTC 1 cut(s) 900
Ppu21I YACGTR 1 cut(s) 799
PsiI TTATAA 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 576
PspCI CACGTG 1 cut(s) 799
PspN4I GGNNCC 4 cut(s) 306, 510, 736, 888
PspPI GGNCC 4 cut(s) 201, 227, 304, 886
PsyI GACNNNGTC 1 cut(s) 592
PteI GCGCGC 1 cut(s) 805
PvuI CGATCG 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 2 cut(s) 501, 713
RsaNI GTAC 2 cut(s) 500, 712
Rsr2I CGGWCCG 2 cut(s) 201, 227
RsrII CGGWCCG 2 cut(s) 201, 227
SacI GAGCTC 1 cut(s) 576
SacII CCGCGG 1 cut(s) 226
SatI GCNGC 6 cut(s) 60, 223, 463, 495, 562, 872
Sau3AI GATC 4 cut(s) 10, 30, 37, 726
Sau96I GGNCC 4 cut(s) 201, 227, 304, 886
SchI GAGTC 1 cut(s) 900
ScrFI CCNGG 3 cut(s) 302, 506, 890
SduI GDGCHC 3 cut(s) 576, 737, 855
SfaNI GCATC 2 cut(s) 97, 593
SfoI GGCGCC 1 cut(s) 510
Sfr303I CCGCGG 1 cut(s) 226
SfuI TTCGAA 1 cut(s) 668
SgrBI CCGCGG 1 cut(s) 226
SinI GGWCC 4 cut(s) 201, 227, 304, 886
Sse9I AATT 1 cut(s) 376
SspDI GGCGCC 1 cut(s) 508
SspMI CTAG 1 cut(s) 243
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 3 cut(s) 300, 504, 888
StyI CCWWGG 2 cut(s) 48, 630
TaaI ACNGT 3 cut(s) 163, 646, 857
TaiI ACGT 1 cut(s) 801
TaqI TCGA 6 cut(s) 9, 33, 135, 601, 668, 775
TaqII GACCGA 1 cut(s) 874
TasI AATT 1 cut(s) 376
TauI GCSGC 2 cut(s) 62, 225
TfiI GAWTC 1 cut(s) 248
TseFI GTSAC 3 cut(s) 206, 445, 857
TseI GCWGC 4 cut(s) 462, 494, 561, 871
Tsp45I GTSAC 3 cut(s) 206, 445, 857
TspDTI ATGAA 1 cut(s) 172
TspGWI ACGGA 3 cut(s) 86, 178, 433
Tth111I GACNNNGTC 1 cut(s) 592
VpaK11BI GGWCC 4 cut(s) 201, 227, 304, 886
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.