pycom13g21150
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002334
Physical Location & Seq
Reverse (-)
17370895 .. 17371953
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g21150.2

Sequence Viewer

Length: 834 bp
ATGGCTATAGATCGATCTGGCTTCTCCAAGATTGACGATCGGACTGCCGTGCAAGAAGCTGCCTCCGCCGGCCTGCAAAGCATGCAGCACCTCATCCGCACCCTGTCCAATCAAACCCCGTCCCACACTCCACTCGATTGCCGGGAAATCACCGACTTCACCGTCACCAAGTTCAAGCACCTAATCTCCGTCTTGAACCGGACCGGTCACGCCCGGTTCCGCCGCGGACCGGCCAAACCAGCTTCAGATTCGGTCCATCCGAAACCTCAGACTACTTTGACTGCTTTTCAAACCCCAAAATCGGACAAGGACAACTCCACTACTGTGTCCCCGCCGGTCTCCACTACGTCGTCGTTTTTTTCCTCAAACACAATCGGAGACGGCAGCGTTTCCAACGGTAAACCCTTCTCATCGATTTCTGTGCCTACGCCTCCGGCTTTCTCCGCCGGAAAACCGCCCCTTCCACAGTCTCACCGGAAACGGTGTCACGAGGGCGAACCCGCTAAAACGTCGTCGTCGTCGGGCCACTGCCATTGTTCTAAAAGAAGGAAGTGTAAGGTTAAGAGGACGATTAGAGTGCCGGCGATTAGCTCGAAAACCGCCGATATACCTGCGGACGAGTTCACCTGGAGAAAGTATGGTCAAAAGCCGATCAAGGGCTCACCTTACCCGAGGTGCAGTACAGTGAGAGGGTGTCCAGCAAGGAAACACGTGGAGAGAGCACAGGACGACCCCAAGATGCTCGTCGTTACCTACGAAGCTGAGCACCGTCACCCGCACCCTTGCCCTTCCCTCACAGCCGCTAATGTGGGCCCTGTTTTCCAGTCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plant_zn_clust WRKY

Protein Analysis

278

Amino Acids

30.19

Weight (kDa)

9.97

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 619
AccII CGCG 1 cut(s) 225
AcoI YGGCCR 1 cut(s) 231
AcuI CTGAAG 1 cut(s) 228
AcvI CACGTG 1 cut(s) 712
AfaI GTAC 1 cut(s) 682
AfiI CCNNNNNNNGG 3 cut(s) 229, 301, 656
AflIII ACRYGT 1 cut(s) 709
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 3 cut(s) 175, 196, 290
AjnI CCWGG 1 cut(s) 626
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 4 cut(s) 59, 242, 591, 761
AluI AGCT 4 cut(s) 59, 242, 591, 761
Alw21I GWGCWC 2 cut(s) 724, 768
Alw26I GTCTC 3 cut(s) 343, 372, 474
Ama87I CYCGRG 1 cut(s) 670
AoxI GGCC 4 cut(s) 70, 231, 523, 811
ApaI GGGCCC 1 cut(s) 815
ApeKI GCWGC 3 cut(s) 59, 85, 384
AsiGI ACCGGT 1 cut(s) 203
AspS9I GGNCC 6 cut(s) 201, 227, 253, 523, 811, 812
AsuC2I CCSGG 2 cut(s) 143, 214
AsuHPI GGTGA 7 cut(s) 142, 151, 157, 464, 616, 654, 764
AvaI CYCGRG 1 cut(s) 670
AvaII GGWCC 3 cut(s) 201, 227, 253
BaeGI GKGCMC 1 cut(s) 815
BanII GRGCYC 2 cut(s) 662, 815
BauI CACGAG 1 cut(s) 488
BbrPI CACGTG 1 cut(s) 712
BbsI GAAGAC 1 cut(s) 819
Bbv12I GWGCWC 2 cut(s) 724, 768
BbvI GCAGC 3 cut(s) 46, 97, 396
BccI CCATC 1 cut(s) 264
BceAI ACGGC 2 cut(s) 32, 397
BcgI CGANNNNNNTGC 8 cut(s) 26, 60, 403, 437, 559, 593, 593, 627
BciT130I CCWGG 1 cut(s) 628
BcnI CCSGG 2 cut(s) 143, 214
BcoDI GTCTC 3 cut(s) 343, 372, 474
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 619
BisI GCNGC 5 cut(s) 60, 86, 223, 385, 801
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 5 cut(s) 61, 87, 224, 386, 802
Bme1390I CCNGG 3 cut(s) 143, 214, 628
Bme18I GGWCC 3 cut(s) 201, 227, 253
BmeT110I CYCGRG 1 cut(s) 670
BmgT120I GGNCC 6 cut(s) 201, 227, 253, 523, 811, 812
BmiI GGNNCC 2 cut(s) 218, 813
BmrFI CCNGG 3 cut(s) 143, 214, 628
BmsI GCATC 1 cut(s) 729
BpiI GAAGAC 1 cut(s) 819
BpmI CTGGAG 1 cut(s) 649
Bpu1102I GCTNAGC 1 cut(s) 762
BpuMI CCSGG 2 cut(s) 143, 214
Bsa29I ATCGAT 2 cut(s) 13, 413
BsaAI YACGTR 1 cut(s) 712
BsaI GGTCTC 1 cut(s) 343
BsaJI CCNNGG 2 cut(s) 223, 671
BsaWI WCCGGW 3 cut(s) 198, 203, 474
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 301, 656
Bse118I RCCGGY 5 cut(s) 68, 203, 229, 334, 580
Bse1I ACTGG 1 cut(s) 823
BseBI CCWGG 1 cut(s) 628
BseCI ATCGAT 2 cut(s) 13, 413
BseDI CCNNGG 2 cut(s) 223, 671
BseGI GGATG 2 cut(s) 93, 256
BseLI CCNNNNNNNGG 3 cut(s) 229, 301, 656
BseMII CTCAG 2 cut(s) 281, 753
BseNI ACTGG 1 cut(s) 823
BseSI GKGCMC 1 cut(s) 815
BseXI GCAGC 3 cut(s) 46, 97, 396
BsgI GTGCAG 1 cut(s) 697
Bsh1236I CGCG 1 cut(s) 225
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 4 cut(s) 72, 233, 525, 813
BshTI ACCGGT 1 cut(s) 203
BshVI ATCGAT 2 cut(s) 13, 413
BsiEI CGRYCG 1 cut(s) 40
BsiHKAI GWGCWC 2 cut(s) 724, 768
BsiHKCI CYCGRG 1 cut(s) 670
BslFI GGGAC 2 cut(s) 106, 313
BslI CCNNNNNNNGG 3 cut(s) 229, 301, 656
BsmAI GTCTC 3 cut(s) 343, 372, 474
BsmBI CGTCTC 1 cut(s) 372
BsmFI GGGAC 2 cut(s) 106, 313
BsnI GGCC 4 cut(s) 72, 233, 525, 813
Bso31I GGTCTC 1 cut(s) 343
BsoBI CYCGRG 1 cut(s) 670
Bsp120I GGGCCC 1 cut(s) 811
Bsp1286I GDGCHC 4 cut(s) 662, 724, 768, 815
Bsp143I GATC 4 cut(s) 10, 14, 37, 651
Bsp1720I GCTNAGC 1 cut(s) 762
BspANI GGCC 4 cut(s) 72, 233, 525, 813
BspCNI CTCAG 2 cut(s) 280, 754
BspDI ATCGAT 2 cut(s) 13, 413
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 2 cut(s) 218, 813
BspMI ACCTGC 1 cut(s) 619
BspTNI GGTCTC 1 cut(s) 343
BsrFI RCCGGY 5 cut(s) 68, 203, 229, 334, 580
BsrI ACTGG 1 cut(s) 823
BssAI RCCGGY 5 cut(s) 68, 203, 229, 334, 580
BssECI CCNNGG 2 cut(s) 223, 671
BssMI GATC 4 cut(s) 10, 14, 37, 651
BssSI CACGAG 1 cut(s) 488
Bst2BI CACGAG 1 cut(s) 488
Bst2UI CCWGG 1 cut(s) 628
Bst4CI ACNGT 7 cut(s) 163, 325, 398, 468, 483, 685, 770
BstAPI GCANNNNNTGC 1 cut(s) 82
BstBAI YACGTR 1 cut(s) 712
BstC8I GCNNGC 4 cut(s) 70, 74, 83, 582
BstDEI CTNAG 2 cut(s) 267, 762
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 2 cut(s) 93, 256
BstFNI CGCG 1 cut(s) 225
BstKTI GATC 4 cut(s) 13, 17, 40, 654
BstMAI GTCTC 3 cut(s) 343, 372, 474
BstMBI GATC 4 cut(s) 10, 14, 37, 651
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 5 cut(s) 65, 78, 82, 239, 443
BstNI CCWGG 1 cut(s) 628
BstNSI RCATGY 1 cut(s) 85
BstSCI CCNGG 3 cut(s) 141, 212, 626
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 815
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 3 cut(s) 46, 97, 396
BstV2I GAAGAC 1 cut(s) 819
Bsu15I ATCGAT 2 cut(s) 13, 413
BsuRI GGCC 4 cut(s) 72, 233, 525, 813
BsuTUI ATCGAT 2 cut(s) 13, 413
BtgI CCRYGG 1 cut(s) 223
BtsCI GGATG 2 cut(s) 93, 256
BtsI GCAGTG 1 cut(s) 526
BtsIMutI CAGTG 2 cut(s) 526, 690
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 4 cut(s) 70, 74, 83, 582
Cfr10I RCCGGY 5 cut(s) 68, 203, 229, 334, 580
Cfr13I GGNCC 6 cut(s) 201, 227, 253, 523, 811, 812
Cfr42I CCGCGG 1 cut(s) 226
ClaI ATCGAT 2 cut(s) 13, 413
CpoI CGGWCCG 2 cut(s) 201, 227
Csp6I GTAC 1 cut(s) 681
CspAI ACCGGT 1 cut(s) 203
CspCI CAANNNNNGTGG 2 cut(s) 515, 550
CspI CGGWCCG 2 cut(s) 201, 227
CviAII CATG 1 cut(s) 82
CviQI GTAC 1 cut(s) 681
DdeI CTNAG 2 cut(s) 267, 762
DpnI GATC 4 cut(s) 12, 16, 39, 653
DpnII GATC 4 cut(s) 10, 14, 37, 651
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 1 cut(s) 231
EciI GGCGGA 3 cut(s) 55, 209, 433
Eco24I GRGCYC 2 cut(s) 662, 815
Eco31I GGTCTC 1 cut(s) 343
Eco47I GGWCC 3 cut(s) 201, 227, 253
Eco57I CTGAAG 1 cut(s) 228
Eco72I CACGTG 1 cut(s) 712
Eco88I CYCGRG 1 cut(s) 670
EcoO109I RGGNCCY 1 cut(s) 812
EcoRII CCWGG 1 cut(s) 626
EcoT38I GRGCYC 2 cut(s) 662, 815
Esp3I CGTCTC 1 cut(s) 372
FaeI CATG 1 cut(s) 85
FaiI YATR 4 cut(s) 8, 83, 608, 639
FaqI GGGAC 2 cut(s) 106, 313
FatI CATG 1 cut(s) 81
FauI CCCGC 3 cut(s) 339, 508, 783
Fnu4HI GCNGC 5 cut(s) 60, 86, 223, 385, 801
FokI GGATG 2 cut(s) 80, 243
FriOI GRGCYC 2 cut(s) 662, 815
Fsp4HI GCNGC 5 cut(s) 60, 86, 223, 385, 801
GluI GCNGC 5 cut(s) 60, 86, 223, 385, 801
GsuI CTGGAG 1 cut(s) 649
HaeIII GGCC 4 cut(s) 72, 233, 525, 813
Hin1II CATG 1 cut(s) 85
HinfI GANTC 1 cut(s) 248
HphI GGTGA 7 cut(s) 142, 151, 157, 464, 616, 654, 764
Hpy166II GTNNAC 2 cut(s) 401, 624
Hpy188I TCNGA 6 cut(s) 42, 247, 261, 270, 304, 377
Hpy188III TCNNGA 3 cut(s) 193, 488, 831
Hpy8I GTNNAC 2 cut(s) 401, 624
Hpy99I CGWCG 7 cut(s) 352, 355, 514, 517, 520, 523, 749
HpyAV CCTTC 4 cut(s) 415, 470, 540, 798
HpyCH4III ACNGT 7 cut(s) 163, 325, 398, 468, 483, 685, 770
HpyCH4IV ACGT 3 cut(s) 347, 509, 711
HpyCH4V TGCA 4 cut(s) 52, 76, 85, 678
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 78, 82, 239, 443
HpyF3I CTNAG 2 cut(s) 267, 762
HpySE526I ACGT 3 cut(s) 347, 509, 711
Hsp92II CATG 1 cut(s) 85
KroI GCCGGC 2 cut(s) 68, 580
KroNI GCCGGC 2 cut(s) 70, 582
KspI CCGCGG 1 cut(s) 226
Kzo9I GATC 4 cut(s) 10, 14, 37, 651
Lsp1109I GCAGC 3 cut(s) 46, 97, 396
LweI GCATC 1 cut(s) 729
MaeII ACGT 3 cut(s) 347, 509, 711
MaeIII GTNAC 5 cut(s) 163, 206, 485, 748, 770
MalI GATC 4 cut(s) 12, 16, 39, 653
MboI GATC 4 cut(s) 10, 14, 37, 651
MboII GAAGA 1 cut(s) 819
MhlI GDGCHC 4 cut(s) 662, 724, 768, 815
MmeI TCCRAC 1 cut(s) 417
MroNI GCCGGC 2 cut(s) 68, 580
MseI TTAA 1 cut(s) 561
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 225
MspR9I CCNGG 3 cut(s) 143, 214, 628
MvaI CCWGG 1 cut(s) 628
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 5 cut(s) 65, 78, 82, 239, 443
NaeI GCCGGC 2 cut(s) 70, 582
NciI CCSGG 2 cut(s) 143, 214
NdeII GATC 4 cut(s) 10, 14, 37, 651
NgoMIV GCCGGC 2 cut(s) 68, 580
NlaIII CATG 1 cut(s) 85
NlaIV GGNNCC 2 cut(s) 218, 813
NmuCI GTSAC 4 cut(s) 163, 206, 485, 770
NspI RCATGY 1 cut(s) 85
OliI CACNNNNGTG 1 cut(s) 323
PaeI GCATGC 1 cut(s) 85
PcsI WCGNNNNNNNCGW 3 cut(s) 257, 515, 753
PdiI GCCGGC 2 cut(s) 70, 582
PfeI GAWTC 1 cut(s) 248
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 5 cut(s) 61, 87, 224, 386, 802
Ple19I CGATCG 1 cut(s) 40
PmaCI CACGTG 1 cut(s) 712
PmlI CACGTG 1 cut(s) 712
Ppu21I YACGTR 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 626
PspCI CACGTG 1 cut(s) 712
PspGI CCWGG 1 cut(s) 626
PspN4I GGNNCC 2 cut(s) 218, 813
PspOMI GGGCCC 1 cut(s) 811
PspPI GGNCC 6 cut(s) 201, 227, 253, 523, 811, 812
PvuI CGATCG 1 cut(s) 40
RsaI GTAC 1 cut(s) 682
RsaNI GTAC 1 cut(s) 681
RseI CAYNNNNRTG 1 cut(s) 323
Rsr2I CGGWCCG 2 cut(s) 201, 227
RsrII CGGWCCG 2 cut(s) 201, 227
SacII CCGCGG 1 cut(s) 226
SaqAI TTAA 1 cut(s) 561
SatI GCNGC 5 cut(s) 60, 86, 223, 385, 801
Sau3AI GATC 4 cut(s) 10, 14, 37, 651
Sau96I GGNCC 6 cut(s) 201, 227, 253, 523, 811, 812
ScrFI CCNGG 3 cut(s) 143, 214, 628
SduI GDGCHC 4 cut(s) 662, 724, 768, 815
SfaNI GCATC 1 cut(s) 729
SfcI CTRYAG 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 226
SgrBI CCGCGG 1 cut(s) 226
SinI GGWCC 3 cut(s) 201, 227, 253
SmiMI CAYNNNNRTG 1 cut(s) 323
SphI GCATGC 1 cut(s) 85
StyD4I CCNGG 3 cut(s) 141, 212, 626
TaaI ACNGT 7 cut(s) 163, 325, 398, 468, 483, 685, 770
TaiI ACGT 3 cut(s) 350, 512, 714
TaqI TCGA 4 cut(s) 13, 135, 413, 593
TaqII GACCGA 1 cut(s) 241
TatI WGTACW 1 cut(s) 680
TauI GCSGC 2 cut(s) 225, 803
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 561
Tru9I TTAA 1 cut(s) 561
TscAI CASTG 2 cut(s) 533, 690
TseFI GTSAC 4 cut(s) 163, 206, 485, 770
TseI GCWGC 3 cut(s) 59, 85, 384
Tsp45I GTSAC 4 cut(s) 163, 206, 485, 770
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 2 cut(s) 533, 690
VpaK11BI GGWCC 3 cut(s) 201, 227, 253
XceI RCATGY 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.