Rmu_co8161446.1_g000001
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8161446.1
OG0002334
Physical Location & Seq
Forward (+)
1 .. 593
593 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8161446.1_g000001.1.cds

Sequence Viewer

Length: 372 bp
aaacgctgccacgacgagctcgcaaccaaaacgacatcgtccggtcgatgccattgttctaaaagaagaaaatccaaggtgaagaggacagtccgagtgccggcgattagttcgaaaatcgccgacataccttccgacgagttttcgtggaggaagtacggccaaaagccgatcaagggctccccatatccacgaggctattataagtgtagtagcgtgcgagggtgtccagcaagaaaacacgtggagcgcgcgcaagacgaccccaccatgcttatcgttacctacgaagcggagcaccgtcaccctcaccctcagcagtcaaactctgtcccggccggtgctgtcggactcgtgttccaatccgcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plant_zn_clust WRKY

Protein Analysis

123

Amino Acids

13.81

Weight (kDa)

9.99

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 204
AccII CGCG 2 cut(s) 252, 254
AciI CCGC 2 cut(s) 293, 366
AcoI YGGCCR 2 cut(s) 160, 336
AcvI CACGTG 1 cut(s) 244
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 2 cut(s) 100, 176
AflIII ACRYGT 1 cut(s) 241
AluBI AGCT 1 cut(s) 19
AluI AGCT 1 cut(s) 19
Alw21I GWGCWC 2 cut(s) 21, 300
AoxI GGCC 2 cut(s) 160, 336
ApeKI GCWGC 1 cut(s) 6
AspLEI GCGC 3 cut(s) 252, 254, 256
AsuC2I CCSGG 1 cut(s) 335
AsuHPI GGTGA 3 cut(s) 91, 296, 302
AsuII TTCGAA 1 cut(s) 113
BanII GRGCYC 2 cut(s) 21, 182
BauI CACGAG 2 cut(s) 192, 353
BbrPI CACGTG 1 cut(s) 244
Bbv12I GWGCWC 2 cut(s) 21, 300
BbvCI CCTCAGC 1 cut(s) 315
BceAI ACGGC 1 cut(s) 175
BcnI CCSGG 1 cut(s) 335
BisI GCNGC 1 cut(s) 7
BlsI GCNGC 1 cut(s) 8
Bme1390I CCNGG 1 cut(s) 335
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 1 cut(s) 335
BmsI GCATC 1 cut(s) 38
Bpu10I CCTNAGC 1 cut(s) 315
Bpu14I TTCGAA 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 335
BsaAI YACGTR 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 75
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 176
Bse118I RCCGGY 2 cut(s) 100, 338
BseDI CCNNGG 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 100, 176
BseMII CTCAG 1 cut(s) 329
BsePI GCGCGC 2 cut(s) 250, 252
BseX3I CGGCCG 1 cut(s) 336
Bsh1236I CGCG 2 cut(s) 252, 254
Bsh1285I CGRYCG 2 cut(s) 46, 339
BshFI GGCC 2 cut(s) 162, 338
BsiEI CGRYCG 2 cut(s) 46, 339
BsiHKAI GWGCWC 2 cut(s) 21, 300
BsiSI CCGG 4 cut(s) 42, 101, 335, 339
BslFI GGGAC 1 cut(s) 317
BslI CCNNNNNNNGG 2 cut(s) 100, 176
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 2 cut(s) 162, 338
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 3 cut(s) 21, 182, 300
Bsp143I GATC 1 cut(s) 171
BspACI CCGC 2 cut(s) 293, 366
BspANI GGCC 2 cut(s) 162, 338
BspCNI CTCAG 1 cut(s) 328
BspFNI CGCG 2 cut(s) 252, 254
BspLI GGNNCC 1 cut(s) 181
BspT104I TTCGAA 1 cut(s) 113
BsrFI RCCGGY 2 cut(s) 100, 338
BssAI RCCGGY 2 cut(s) 100, 338
BssECI CCNNGG 1 cut(s) 75
BssHII GCGCGC 2 cut(s) 250, 252
BssMI GATC 1 cut(s) 171
BssSI CACGAG 2 cut(s) 192, 353
BssT1I CCWWGG 1 cut(s) 75
Bst2BI CACGAG 2 cut(s) 192, 353
Bst4CI ACNGT 2 cut(s) 91, 302
Bst6I CTCTTC 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 244
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 5 cut(s) 21, 102, 218, 252, 254
BstDEI CTNAG 1 cut(s) 315
BstFNI CGCG 2 cut(s) 252, 254
BstHHI GCGC 3 cut(s) 252, 254, 256
BstKTI GATC 1 cut(s) 174
BstMBI GATC 1 cut(s) 171
BstMCI CGRYCG 2 cut(s) 46, 339
BstSCI CCNGG 1 cut(s) 333
BstUI CGCG 2 cut(s) 252, 254
BstZI CGGCCG 1 cut(s) 336
BsuRI GGCC 2 cut(s) 162, 338
Cac8I GCNNGC 5 cut(s) 21, 102, 218, 252, 254
CfoI GCGC 3 cut(s) 252, 254, 256
Cfr10I RCCGGY 2 cut(s) 100, 338
Csp6I GTAC 1 cut(s) 157
CviAII CATG 1 cut(s) 271
CviJI RGCY 6 cut(s) 19, 162, 169, 180, 198, 338
CviKI_1 RGCY 6 cut(s) 19, 162, 169, 180, 198, 338
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 1 cut(s) 315
DpnI GATC 1 cut(s) 173
DpnII GATC 1 cut(s) 171
EaeI YGGCCR 2 cut(s) 160, 336
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
EciI GGCGGA 1 cut(s) 355
Ecl136II GAGCTC 1 cut(s) 19
EclXI CGGCCG 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 2 cut(s) 21, 182
Eco52I CGGCCG 1 cut(s) 336
Eco53kI GAGCTC 1 cut(s) 19
Eco72I CACGTG 1 cut(s) 244
EcoICRI GAGCTC 1 cut(s) 19
EcoT14I CCWWGG 1 cut(s) 75
EcoT38I GRGCYC 2 cut(s) 21, 182
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 1 cut(s) 274
FaiI YATR 4 cut(s) 128, 187, 204, 272
FaqI GGGAC 1 cut(s) 317
FatI CATG 1 cut(s) 270
Fnu4HI GCNGC 1 cut(s) 7
FriOI GRGCYC 2 cut(s) 21, 182
Fsp4HI GCNGC 1 cut(s) 7
GlaI GCGC 3 cut(s) 251, 253, 255
GluI GCNGC 1 cut(s) 7
HaeIII GGCC 2 cut(s) 162, 338
HapII CCGG 4 cut(s) 42, 101, 335, 339
HhaI GCGC 3 cut(s) 252, 254, 256
Hin1II CATG 1 cut(s) 274
Hin6I GCGC 3 cut(s) 250, 252, 254
HinP1I GCGC 3 cut(s) 250, 252, 254
HinfI GANTC 1 cut(s) 351
HpaII CCGG 4 cut(s) 42, 101, 335, 339
HphI GGTGA 3 cut(s) 91, 296, 302
Hpy188I TCNGA 3 cut(s) 95, 136, 350
Hpy99I CGWCG 2 cut(s) 17, 140
HpyAV CCTTC 1 cut(s) 141
HpyCH4III ACNGT 2 cut(s) 91, 302
HpyCH4IV ACGT 1 cut(s) 243
HpyF3I CTNAG 1 cut(s) 315
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 1 cut(s) 274
HspAI GCGC 3 cut(s) 250, 252, 254
KroI GCCGGC 1 cut(s) 100
KroNI GCCGGC 1 cut(s) 102
Kzo9I GATC 1 cut(s) 171
LmnI GCTCC 3 cut(s) 185, 247, 295
LpnPI CCDG 5 cut(s) 55, 114, 243, 348, 352
LweI GCATC 1 cut(s) 38
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 2 cut(s) 280, 302
MalI GATC 1 cut(s) 173
MboI GATC 1 cut(s) 171
MboII GAAGA 2 cut(s) 78, 94
MhlI GDGCHC 3 cut(s) 21, 182, 300
MlyI GAGTC 1 cut(s) 345
MmeI TCCRAC 2 cut(s) 159, 328
MnlI CCTC 6 cut(s) 78, 144, 188, 215, 318, 324
MroNI GCCGGC 1 cut(s) 100
MspI CCGG 4 cut(s) 42, 101, 335, 339
MspR9I CCNGG 1 cut(s) 335
MvnI CGCG 2 cut(s) 252, 254
NaeI GCCGGC 1 cut(s) 102
NciI CCSGG 1 cut(s) 335
NdeII GATC 1 cut(s) 171
NgoMIV GCCGGC 1 cut(s) 100
NlaIII CATG 1 cut(s) 274
NlaIV GGNNCC 1 cut(s) 181
NmuCI GTSAC 1 cut(s) 302
NspV TTCGAA 1 cut(s) 113
PauI GCGCGC 2 cut(s) 250, 252
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PdiI GCCGGC 1 cut(s) 102
PflFI GACNNNGTC 1 cut(s) 37
PkrI GCNGC 1 cut(s) 8
PleI GAGTC 1 cut(s) 345
PmaCI CACGTG 1 cut(s) 244
PmlI CACGTG 1 cut(s) 244
PpsI GAGTC 1 cut(s) 345
Ppu21I YACGTR 1 cut(s) 244
PsiI TTATAA 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 21
PspCI CACGTG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 181
PsyI GACNNNGTC 1 cut(s) 37
PteI GCGCGC 2 cut(s) 250, 252
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
SacI GAGCTC 1 cut(s) 21
SatI GCNGC 1 cut(s) 7
Sau3AI GATC 1 cut(s) 171
SchI GAGTC 1 cut(s) 345
ScrFI CCNGG 1 cut(s) 335
SduI GDGCHC 3 cut(s) 21, 182, 300
SetI ASST 5 cut(s) 21, 81, 133, 246, 287
SfaNI GCATC 1 cut(s) 38
SfuI TTCGAA 1 cut(s) 113
SsiI CCGC 2 cut(s) 293, 366
SstI GAGCTC 1 cut(s) 21
StyD4I CCNGG 1 cut(s) 333
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 2 cut(s) 91, 302
TaiI ACGT 1 cut(s) 246
TaqI TCGA 2 cut(s) 46, 113
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 1 cut(s) 6
Tsp45I GTSAC 1 cut(s) 302
Tth111I GACNNNGTC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.