RchiOBHm_Chr4g0398741
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002334
Physical Location & Seq
Reverse (-)
15388947 .. 15390452
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37090

Sequence Viewer

Length: 918 bp
ATGGCAATTGATCTCGTTAGGTTCTCCAAGATCGACGATCGGACGGCCATGGAAGAGGCCGCCTCCGCTGGCTTACAGAGCATGGAGCATCTCATCCGTGTCTTGTCCAGTCAAACCCCGTCTCACAACAACCTCGACTGCGGTGAAATCACCGACTTCACCGTCGCTAAGTTCAAGCAGTTCATCTCCGTCTTGAACCGGACCGGTCACGCCCGCTTCCGCCGCGGACCGGTCAAGCAACCTAGTGACTCTACCACTACTTCTTCTCCTCCTCCTCCACCTCCTCCCAACCCGGTCCCACCAAAGCCTCAGGTTACTTTGTCTTTGGAAACCGGAAATCCCAATACCAATACCAATACGAACACCCATTCCAAGTCCAAAGACTGCCTCACCTTATCTCCTCCCATGTCCACAACGTCATCGTTTCTTTCTTCCATCACCACCGGAGACGGCAGCGTTTCGAACGGCAAAGTTTTCTCCTCAGCTGCCTTTCCCCCGGCTCCGGCCTTTTCCGCCGGTAAACCCCCGCTCTCCCAATCCCACCGGAAACGCTGCCACGACGAGCTCGCAACCAAAACGACATCGTCCGGTCGATGCCATTGTTCTAAAAGAAGAAAATCCAAGGTGAAGAGGACAGTCCGAGTGCCGGCGATTAGTTCGAAAATCGCCGACATACCTTCCGACGAGTTTTCGTGGAGGAAGTACGGCCAAAAGCCGATCAAGGGCTCCCCATACCCACGAGGCTATTATAAGTGTAGTAGCGTGCGAGGGTGTCCAGCAAGAAAACACGTGGAGCGCGCGCAAGACGACCCCACCATGCTTATCGTTACCTACGAAGCGGAGCACCGTCACCCTCACCCGCAGCAGTCAAACTCTGTCCCGGCGGGTGTTGTCGGACTCGTGTTCCAATCCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plant_zn_clust WRKY

Protein Analysis

305

Amino Acids

33.16

Weight (kDa)

9.85

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_zn_clust PF10533 182 - 224 1.9e-17 Plant zinc cluster domain
WRKY PF03106 228 - 285 3.4e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
AasI GACNNNNNNGTC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 529
AccII CGCG 3 cut(s) 225, 798, 800
AcoI YGGCCR 2 cut(s) 45, 706
AcvI CACGTG 1 cut(s) 790
AfaI GTAC 1 cut(s) 704
AfiI CCNNNNNNNGG 4 cut(s) 229, 502, 646, 722
AflIII ACRYGT 1 cut(s) 787
AgeI ACCGGT 2 cut(s) 203, 229
AgsI TTSAA 2 cut(s) 175, 196
AloI GAACNNNNNNTCC 2 cut(s) 353, 385
AluBI AGCT 2 cut(s) 485, 565
AluI AGCT 2 cut(s) 485, 565
Alw21I GWGCWC 2 cut(s) 567, 846
Alw26I GTCTC 2 cut(s) 126, 441
AoxI GGCC 4 cut(s) 45, 57, 504, 706
ApeKI GCWGC 4 cut(s) 453, 485, 552, 862
AsiGI ACCGGT 2 cut(s) 203, 229
AspLEI GCGC 3 cut(s) 798, 800, 802
AspS9I GGNCC 3 cut(s) 201, 227, 295
AsuC2I CCSGG 3 cut(s) 293, 497, 881
AsuHPI GGTGA 8 cut(s) 142, 151, 155, 382, 430, 637, 842, 848
AsuII TTCGAA 2 cut(s) 461, 659
AvaII GGWCC 3 cut(s) 201, 227, 295
AxyI CCTNAGG 1 cut(s) 309
BanII GRGCYC 2 cut(s) 567, 728
BauI CACGAG 2 cut(s) 738, 899
BbrPI CACGTG 1 cut(s) 790
Bbv12I GWGCWC 2 cut(s) 567, 846
BbvCI CCTCAGC 1 cut(s) 481
BbvI GCAGC 4 cut(s) 465, 472, 539, 874
BccI CCATC 1 cut(s) 443
BceAI ACGGC 4 cut(s) 60, 466, 481, 721
BcnI CCSGG 3 cut(s) 293, 497, 881
BcoDI GTCTC 2 cut(s) 126, 441
BfaI CTAG 1 cut(s) 243
BisI GCNGC 6 cut(s) 60, 223, 454, 486, 553, 863
BlsI GCNGC 6 cut(s) 61, 224, 455, 487, 554, 864
Bme1390I CCNGG 3 cut(s) 293, 497, 881
Bme18I GGWCC 3 cut(s) 201, 227, 295
BmgT120I GGNCC 3 cut(s) 201, 227, 295
BmiI GGNNCC 3 cut(s) 297, 501, 727
BmrFI CCNGG 3 cut(s) 293, 497, 881
BmsI GCATC 2 cut(s) 97, 584
BplI GAGNNNNNCTC 2 cut(s) 47, 79
Bpu10I CCTNAGC 1 cut(s) 481
Bpu14I TTCGAA 2 cut(s) 461, 659
BpuMI CCSGG 3 cut(s) 293, 497, 881
BsaAI YACGTR 1 cut(s) 790
BsaJI CCNNGG 4 cut(s) 48, 223, 495, 621
BsaWI WCCGGW 7 cut(s) 198, 203, 229, 332, 443, 543, 587
BsaXI ACNNNNNCTCC 4 cut(s) 250, 280, 382, 412
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 502, 646, 722
Bse118I RCCGGY 4 cut(s) 203, 229, 515, 646
Bse1I ACTGG 1 cut(s) 108
Bse21I CCTNAGG 1 cut(s) 309
BseDI CCNNGG 4 cut(s) 48, 223, 495, 621
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 4 cut(s) 229, 502, 646, 722
BseMII CTCAG 2 cut(s) 323, 495
BseNI ACTGG 1 cut(s) 108
BsePI GCGCGC 2 cut(s) 796, 798
BseRI GAGGAG 6 cut(s) 258, 261, 264, 273, 390, 469
BseXI GCAGC 4 cut(s) 465, 472, 539, 874
Bsh1236I CGCG 3 cut(s) 225, 798, 800
Bsh1285I CGRYCG 2 cut(s) 40, 592
BshFI GGCC 4 cut(s) 47, 59, 506, 708
BshTI ACCGGT 2 cut(s) 203, 229
BsiEI CGRYCG 2 cut(s) 40, 592
BsiHKAI GWGCWC 2 cut(s) 567, 846
BslFI GGGAC 2 cut(s) 281, 863
BslI CCNNNNNNNGG 4 cut(s) 229, 502, 646, 722
BsmAI GTCTC 2 cut(s) 126, 441
BsmBI CGTCTC 2 cut(s) 126, 441
BsmFI GGGAC 2 cut(s) 281, 863
BsnI GGCC 4 cut(s) 47, 59, 506, 708
Bsp119I TTCGAA 2 cut(s) 461, 659
Bsp1286I GDGCHC 3 cut(s) 567, 728, 846
Bsp143I GATC 4 cut(s) 10, 30, 37, 717
Bsp19I CCATGG 1 cut(s) 48
BspANI GGCC 4 cut(s) 47, 59, 506, 708
BspCNI CTCAG 2 cut(s) 322, 494
BspFNI CGCG 3 cut(s) 225, 798, 800
BspLI GGNNCC 3 cut(s) 297, 501, 727
BspT104I TTCGAA 2 cut(s) 461, 659
BsrBI CCGCTC 1 cut(s) 529
BsrFI RCCGGY 4 cut(s) 203, 229, 515, 646
BsrI ACTGG 1 cut(s) 108
BssAI RCCGGY 4 cut(s) 203, 229, 515, 646
BssECI CCNNGG 4 cut(s) 48, 223, 495, 621
BssHII GCGCGC 2 cut(s) 796, 798
BssMI GATC 4 cut(s) 10, 30, 37, 717
BssSI CACGAG 2 cut(s) 738, 899
BssT1I CCWWGG 2 cut(s) 48, 621
Bst2BI CACGAG 2 cut(s) 738, 899
Bst4CI ACNGT 3 cut(s) 163, 637, 848
Bst6I CTCTTC 2 cut(s) 48, 623
BstBAI YACGTR 1 cut(s) 790
BstBI TTCGAA 2 cut(s) 461, 659
BstC8I GCNNGC 7 cut(s) 70, 214, 567, 648, 764, 798, 800
BstDEI CTNAG 3 cut(s) 168, 309, 481
BstDSI CCRYGG 2 cut(s) 48, 223
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 3 cut(s) 225, 798, 800
BstHHI GCGC 3 cut(s) 798, 800, 802
BstKTI GATC 4 cut(s) 13, 33, 40, 720
BstMAI GTCTC 2 cut(s) 126, 441
BstMBI GATC 4 cut(s) 10, 30, 37, 717
BstMCI CGRYCG 2 cut(s) 40, 592
BstMWI GCNNNNNNNGC 4 cut(s) 65, 78, 222, 512
BstSCI CCNGG 3 cut(s) 291, 495, 879
BstUI CGCG 3 cut(s) 225, 798, 800
BstV1I GCAGC 4 cut(s) 465, 472, 539, 874
Bsu36I CCTNAGG 1 cut(s) 309
BsuRI GGCC 4 cut(s) 47, 59, 506, 708
BtgI CCRYGG 2 cut(s) 48, 223
BtsCI GGATG 1 cut(s) 93
Cac8I GCNNGC 7 cut(s) 70, 214, 567, 648, 764, 798, 800
CfoI GCGC 3 cut(s) 798, 800, 802
Cfr10I RCCGGY 4 cut(s) 203, 229, 515, 646
Cfr13I GGNCC 3 cut(s) 201, 227, 295
Cfr42I CCGCGG 1 cut(s) 226
CpoI CGGWCCG 2 cut(s) 201, 227
Csp6I GTAC 1 cut(s) 703
CspAI ACCGGT 2 cut(s) 203, 229
CspI CGGWCCG 2 cut(s) 201, 227
CviAII CATG 4 cut(s) 49, 82, 406, 817
CviQI GTAC 1 cut(s) 703
DdeI CTNAG 3 cut(s) 168, 309, 481
DpnI GATC 4 cut(s) 12, 32, 39, 719
DpnII GATC 4 cut(s) 10, 30, 37, 717
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 2 cut(s) 45, 706
Eam1104I CTCTTC 2 cut(s) 48, 623
EarI CTCTTC 2 cut(s) 48, 623
EciI GGCGGA 3 cut(s) 209, 502, 901
Ecl136II GAGCTC 1 cut(s) 565
Eco130I CCWWGG 2 cut(s) 48, 621
Eco24I GRGCYC 2 cut(s) 567, 728
Eco47I GGWCC 3 cut(s) 201, 227, 295
Eco53kI GAGCTC 1 cut(s) 565
Eco72I CACGTG 1 cut(s) 790
Eco81I CCTNAGG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 565
EcoT14I CCWWGG 2 cut(s) 48, 621
EcoT38I GRGCYC 2 cut(s) 567, 728
ErhI CCWWGG 2 cut(s) 48, 621
Esp3I CGTCTC 2 cut(s) 126, 441
FaeI CATG 4 cut(s) 52, 85, 409, 820
FaiI YATR 7 cut(s) 50, 83, 407, 674, 733, 750, 818
FaqI GGGAC 2 cut(s) 281, 863
FatI CATG 4 cut(s) 48, 81, 405, 816
FauI CCCGC 4 cut(s) 221, 534, 867, 877
Fnu4HI GCNGC 6 cut(s) 60, 223, 454, 486, 553, 863
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 2 cut(s) 567, 728
Fsp4HI GCNGC 6 cut(s) 60, 223, 454, 486, 553, 863
FspBI CTAG 1 cut(s) 243
GlaI GCGC 3 cut(s) 797, 799, 801
GluI GCNGC 6 cut(s) 60, 223, 454, 486, 553, 863
HaeIII GGCC 4 cut(s) 47, 59, 506, 708
HhaI GCGC 3 cut(s) 798, 800, 802
Hin1II CATG 4 cut(s) 52, 85, 409, 820
Hin6I GCGC 3 cut(s) 796, 798, 800
HinP1I GCGC 3 cut(s) 796, 798, 800
HinfI GANTC 2 cut(s) 248, 897
HphI GGTGA 8 cut(s) 142, 151, 155, 382, 430, 637, 842, 848
Hpy166II GTNNAC 2 cut(s) 411, 521
Hpy188I TCNGA 4 cut(s) 42, 641, 682, 896
Hpy188III TCNNGA 1 cut(s) 193
Hpy8I GTNNAC 2 cut(s) 411, 521
Hpy99I CGWCG 4 cut(s) 38, 167, 563, 686
HpyAV CCTTC 1 cut(s) 687
HpyCH4III ACNGT 3 cut(s) 163, 637, 848
HpyCH4IV ACGT 2 cut(s) 416, 789
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 78, 222, 512
HpyF3I CTNAG 3 cut(s) 168, 309, 481
HpySE526I ACGT 2 cut(s) 416, 789
Hsp92II CATG 4 cut(s) 52, 85, 409, 820
HspAI GCGC 3 cut(s) 796, 798, 800
KroI GCCGGC 1 cut(s) 646
KroNI GCCGGC 1 cut(s) 648
KspI CCGCGG 1 cut(s) 226
Kzo9I GATC 4 cut(s) 10, 30, 37, 717
LmnI GCTCC 5 cut(s) 85, 505, 731, 793, 841
Lsp1109I GCAGC 4 cut(s) 465, 472, 539, 874
LweI GCATC 2 cut(s) 97, 584
MaeI CTAG 1 cut(s) 243
MaeII ACGT 2 cut(s) 416, 789
MaeIII GTNAC 5 cut(s) 206, 245, 313, 826, 848
MalI GATC 4 cut(s) 12, 32, 39, 719
MbiI CCGCTC 1 cut(s) 529
MboI GATC 4 cut(s) 10, 30, 37, 717
MboII GAAGA 5 cut(s) 65, 255, 423, 624, 640
MfeI CAATTG 1 cut(s) 6
MhlI GDGCHC 3 cut(s) 567, 728, 846
MluCI AATT 1 cut(s) 6
MlyI GAGTC 2 cut(s) 242, 891
MmeI TCCRAC 2 cut(s) 705, 874
MroNI GCCGGC 1 cut(s) 646
MspA1I CMGCKG 3 cut(s) 68, 225, 485
MspR9I CCNGG 3 cut(s) 293, 497, 881
MunI CAATTG 1 cut(s) 6
MvnI CGCG 3 cut(s) 225, 798, 800
MwoI GCNNNNNNNGC 4 cut(s) 65, 78, 222, 512
NaeI GCCGGC 1 cut(s) 648
NciI CCSGG 3 cut(s) 293, 497, 881
NcoI CCATGG 1 cut(s) 48
NdeII GATC 4 cut(s) 10, 30, 37, 717
NgoMIV GCCGGC 1 cut(s) 646
NlaIII CATG 4 cut(s) 52, 85, 409, 820
NlaIV GGNNCC 3 cut(s) 297, 501, 727
NmuCI GTSAC 3 cut(s) 206, 245, 848
NspV TTCGAA 2 cut(s) 461, 659
PauI GCGCGC 2 cut(s) 796, 798
PcsI WCGNNNNNNNCGW 1 cut(s) 831
PdiI GCCGGC 1 cut(s) 648
PflFI GACNNNGTC 1 cut(s) 583
PinAI ACCGGT 2 cut(s) 203, 229
PkrI GCNGC 6 cut(s) 61, 224, 455, 487, 554, 864
Ple19I CGATCG 1 cut(s) 40
PleI GAGTC 2 cut(s) 242, 891
PmaCI CACGTG 1 cut(s) 790
PmlI CACGTG 1 cut(s) 790
PpsI GAGTC 2 cut(s) 242, 891
Ppu21I YACGTR 1 cut(s) 790
PsiI TTATAA 1 cut(s) 750
Psp124BI GAGCTC 1 cut(s) 567
PspCI CACGTG 1 cut(s) 790
PspN4I GGNNCC 3 cut(s) 297, 501, 727
PspPI GGNCC 3 cut(s) 201, 227, 295
PsyI GACNNNGTC 1 cut(s) 583
PteI GCGCGC 2 cut(s) 796, 798
PvuI CGATCG 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 1 cut(s) 704
RsaNI GTAC 1 cut(s) 703
Rsr2I CGGWCCG 2 cut(s) 201, 227
RsrII CGGWCCG 2 cut(s) 201, 227
SacI GAGCTC 1 cut(s) 567
SacII CCGCGG 1 cut(s) 226
SatI GCNGC 6 cut(s) 60, 223, 454, 486, 553, 863
Sau3AI GATC 4 cut(s) 10, 30, 37, 717
Sau96I GGNCC 3 cut(s) 201, 227, 295
SchI GAGTC 2 cut(s) 242, 891
ScrFI CCNGG 3 cut(s) 293, 497, 881
SduI GDGCHC 3 cut(s) 567, 728, 846
SfaNI GCATC 2 cut(s) 97, 584
Sfr303I CCGCGG 1 cut(s) 226
SfuI TTCGAA 2 cut(s) 461, 659
SgrBI CCGCGG 1 cut(s) 226
SinI GGWCC 3 cut(s) 201, 227, 295
Sse9I AATT 1 cut(s) 6
SspMI CTAG 1 cut(s) 243
SstI GAGCTC 1 cut(s) 567
StyD4I CCNGG 3 cut(s) 291, 495, 879
StyI CCWWGG 2 cut(s) 48, 621
TaaI ACNGT 3 cut(s) 163, 637, 848
TaiI ACGT 2 cut(s) 419, 792
TaqI TCGA 5 cut(s) 33, 135, 461, 592, 659
TasI AATT 1 cut(s) 6
TauI GCSGC 2 cut(s) 62, 225
TseFI GTSAC 3 cut(s) 206, 245, 848
TseI GCWGC 4 cut(s) 453, 485, 552, 862
Tsp45I GTSAC 3 cut(s) 206, 245, 848
TspDTI ATGAA 1 cut(s) 172
TspGWI ACGGA 2 cut(s) 86, 178
Tth111I GACNNNGTC 1 cut(s) 583
VpaK11BI GGWCC 3 cut(s) 201, 227, 295
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.