Rroxscaffold_5G00343690
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002334
Physical Location & Seq
Reverse (-)
13316993 .. 13318454
1462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00343690.1

Sequence Viewer

Length: 927 bp
ATGGCAATTGATCTAGTTAGGTTCTCCAAGATCGACGATCGAACGGCCATGGAAGAGGCCGCCTCCGCCGGCTTACAGAGCATGGAGCATCTCATCGGTGTCTTGTCCAGTCAAACCCCGTCTCACAACAACCTCGACTGCCGTGAAATCACCGACTTCACCGTCGCCAAGTTCAAGCAGTTCATCTCCGTCTTGAACCGGACCGGTCACGCCCGCTTCCGCCGCGGACCGGCCAAGCAACCTAGTGAATCTACCACCACCTCTTCTCCTCCTCCTCCTCCTCCTCCACCTCCTGCCAACCCGGTCCCACCAAAGCCTCAGGTTACTTTGTCTTTTGAAACCGGAAATCCCAATACCAATACCAATACAAACACCAATTCCAAGTCCAAAGACTGCCTCACCTTATCTCCTCCCATGTCCACAACGTCGTCGTTTCTTTCTTCCGTCACCACCGGAGACGGCAGCGTTTCCAACGGCAAAGTTTTCTCCTCAGCTGCCGTACCCCCGGCGCCGGCCTTCTCCGCCGGCAAACCCCCGCTCGCCCAATCCCACCGGAAACGCTGCCACGACGAGCTCGCAACCAAAACGACATCGTCCTGTCGATGCCATTGTTCTAAAAGAAGAAAATCCAAGGTGAAGAGGACAGTCCGAGTTCCGGCGATTAGTTCGAAAATCGCCGATATCCCTTCCGACGAGTTTTCGTGGAGGAAGTACGGCCAAAAGCCGATCAAGGGCTCCCCATACCCACGAGGCTATTATAAGTGTAGTAGCGTTCGAGGGTGTCCAGCGAGAAAACACGTGGAGCGCGCTCAAGACGACCCCACCATGCTTATCGTTACCTACGAAGCCGAGCACCGTCACCCTCACCCGCAGCAGTCAAACTCAGTCCCGGCCGGTGTTGTGGGACTCGTGTTCCAATCCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plant_zn_clust WRKY

Protein Analysis

308

Amino Acids

33.39

Weight (kDa)

9.79

Isoelectric Point (pI)

53.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_zn_clust PF10533 185 - 227 1.5e-15 Plant zinc cluster domain
WRKY PF03106 231 - 288 3.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
AasI GACNNNNNNGTC 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 508
AccBSI CCGCTC 1 cut(s) 538
AccII CGCG 2 cut(s) 225, 807
AcoI YGGCCR 4 cut(s) 45, 231, 715, 891
AcvI CACGTG 1 cut(s) 799
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 2 cut(s) 501, 713
AfiI CCNNNNNNNGG 4 cut(s) 229, 511, 655, 731
AflIII ACRYGT 1 cut(s) 796
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 3 cut(s) 175, 196, 338
AluBI AGCT 2 cut(s) 494, 574
AluI AGCT 2 cut(s) 494, 574
Alw21I GWGCWC 2 cut(s) 576, 855
Alw26I GTCTC 2 cut(s) 126, 450
AoxI GGCC 6 cut(s) 45, 57, 231, 513, 715, 891
ApeKI GCWGC 4 cut(s) 462, 494, 561, 871
AsiGI ACCGGT 1 cut(s) 203
AspLEI GCGC 3 cut(s) 511, 807, 809
AspS9I GGNCC 3 cut(s) 201, 227, 304
AsuC2I CCSGG 3 cut(s) 302, 506, 890
AsuHPI GGTGA 7 cut(s) 142, 151, 391, 439, 646, 851, 857
AsuII TTCGAA 1 cut(s) 668
AvaII GGWCC 3 cut(s) 201, 227, 304
AxyI CCTNAGG 1 cut(s) 318
BanI GGYRCC 1 cut(s) 508
BanII GRGCYC 2 cut(s) 576, 737
BauI CACGAG 2 cut(s) 747, 908
BbrPI CACGTG 1 cut(s) 799
Bbv12I GWGCWC 2 cut(s) 576, 855
BbvCI CCTCAGC 1 cut(s) 490
BbvI GCAGC 4 cut(s) 474, 481, 548, 883
BceAI ACGGC 6 cut(s) 60, 126, 475, 482, 490, 730
BcnI CCSGG 3 cut(s) 302, 506, 890
BcoDI GTCTC 2 cut(s) 126, 450
BfaI CTAG 2 cut(s) 14, 243
BfoI RGCGCY 1 cut(s) 512
BisI GCNGC 6 cut(s) 60, 223, 463, 495, 562, 872
BlsI GCNGC 6 cut(s) 61, 224, 464, 496, 563, 873
Bme1390I CCNGG 3 cut(s) 302, 506, 890
Bme18I GGWCC 3 cut(s) 201, 227, 304
BmgT120I GGNCC 3 cut(s) 201, 227, 304
BmiI GGNNCC 3 cut(s) 306, 510, 736
BmrFI CCNGG 3 cut(s) 302, 506, 890
BmsI GCATC 2 cut(s) 97, 593
BplI GAGNNNNNCTC 2 cut(s) 47, 79
Bpu10I CCTNAGC 1 cut(s) 490
Bpu14I TTCGAA 1 cut(s) 668
BpuEI CTTGAG 1 cut(s) 795
BpuMI CCSGG 3 cut(s) 302, 506, 890
BsaAI YACGTR 1 cut(s) 799
BsaHI GRCGYC 1 cut(s) 509
BsaJI CCNNGG 4 cut(s) 48, 223, 504, 630
BsaWI WCCGGW 5 cut(s) 198, 203, 341, 452, 552
BsaXI ACNNNNNCTCC 4 cut(s) 250, 280, 391, 421
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 511, 655, 731
Bse118I RCCGGY 6 cut(s) 68, 203, 229, 511, 524, 893
Bse1I ACTGG 1 cut(s) 108
Bse21I CCTNAGG 1 cut(s) 318
BseDI CCNNGG 4 cut(s) 48, 223, 504, 630
BseLI CCNNNNNNNGG 4 cut(s) 229, 511, 655, 731
BseMII CTCAG 3 cut(s) 332, 504, 897
BseNI ACTGG 1 cut(s) 108
BsePI GCGCGC 1 cut(s) 805
BseRI GAGGAG 8 cut(s) 258, 261, 264, 267, 270, 273, 399, 478
BseX3I CGGCCG 1 cut(s) 891
BseXI GCAGC 4 cut(s) 474, 481, 548, 883
Bsh1236I CGCG 2 cut(s) 225, 807
Bsh1285I CGRYCG 2 cut(s) 40, 894
BshFI GGCC 6 cut(s) 47, 59, 233, 515, 717, 893
BshNI GGYRCC 1 cut(s) 508
BshTI ACCGGT 1 cut(s) 203
BsiEI CGRYCG 2 cut(s) 40, 894
BsiHKAI GWGCWC 2 cut(s) 576, 855
BslFI GGGAC 3 cut(s) 290, 872, 918
BslI CCNNNNNNNGG 4 cut(s) 229, 511, 655, 731
BsmAI GTCTC 2 cut(s) 126, 450
BsmBI CGTCTC 2 cut(s) 126, 450
BsmFI GGGAC 3 cut(s) 290, 872, 918
BsnI GGCC 6 cut(s) 47, 59, 233, 515, 717, 893
Bsp119I TTCGAA 1 cut(s) 668
Bsp1286I GDGCHC 3 cut(s) 576, 737, 855
Bsp143I GATC 4 cut(s) 10, 30, 37, 726
Bsp19I CCATGG 1 cut(s) 48
BspANI GGCC 6 cut(s) 47, 59, 233, 515, 717, 893
BspCNI CTCAG 3 cut(s) 331, 503, 896
BspFNI CGCG 2 cut(s) 225, 807
BspLI GGNNCC 3 cut(s) 306, 510, 736
BspT104I TTCGAA 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 508
BsrBI CCGCTC 1 cut(s) 538
BsrFI RCCGGY 6 cut(s) 68, 203, 229, 511, 524, 893
BsrI ACTGG 1 cut(s) 108
BssAI RCCGGY 6 cut(s) 68, 203, 229, 511, 524, 893
BssECI CCNNGG 4 cut(s) 48, 223, 504, 630
BssHII GCGCGC 1 cut(s) 805
BssMI GATC 4 cut(s) 10, 30, 37, 726
BssNI GRCGYC 1 cut(s) 509
BssSI CACGAG 2 cut(s) 747, 908
BssT1I CCWWGG 2 cut(s) 48, 630
Bst2BI CACGAG 2 cut(s) 747, 908
Bst4CI ACNGT 3 cut(s) 163, 646, 857
Bst6I CTCTTC 3 cut(s) 48, 268, 632
BstACI GRCGYC 1 cut(s) 509
BstBAI YACGTR 1 cut(s) 799
BstBI TTCGAA 1 cut(s) 668
BstC8I GCNNGC 7 cut(s) 70, 214, 513, 526, 540, 576, 807
BstDEI CTNAG 3 cut(s) 318, 490, 883
BstDSI CCRYGG 2 cut(s) 48, 223
BstFNI CGCG 2 cut(s) 225, 807
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 3 cut(s) 511, 807, 809
BstKTI GATC 4 cut(s) 13, 33, 40, 729
BstMAI GTCTC 2 cut(s) 126, 450
BstMBI GATC 4 cut(s) 10, 30, 37, 726
BstMCI CGRYCG 2 cut(s) 40, 894
BstMWI GCNNNNNNNGC 4 cut(s) 65, 78, 222, 521
BstSCI CCNGG 3 cut(s) 300, 504, 888
BstUI CGCG 2 cut(s) 225, 807
BstV1I GCAGC 4 cut(s) 474, 481, 548, 883
BstZI CGGCCG 1 cut(s) 891
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 6 cut(s) 47, 59, 233, 515, 717, 893
BtgI CCRYGG 2 cut(s) 48, 223
Cac8I GCNNGC 7 cut(s) 70, 214, 513, 526, 540, 576, 807
CfoI GCGC 3 cut(s) 511, 807, 809
Cfr10I RCCGGY 6 cut(s) 68, 203, 229, 511, 524, 893
Cfr13I GGNCC 3 cut(s) 201, 227, 304
Cfr42I CCGCGG 1 cut(s) 226
CpoI CGGWCCG 2 cut(s) 201, 227
Csp6I GTAC 2 cut(s) 500, 712
CspAI ACCGGT 1 cut(s) 203
CspI CGGWCCG 2 cut(s) 201, 227
CviAII CATG 4 cut(s) 49, 82, 415, 826
CviQI GTAC 2 cut(s) 500, 712
DdeI CTNAG 3 cut(s) 318, 490, 883
DinI GGCGCC 1 cut(s) 510
DpnI GATC 4 cut(s) 12, 32, 39, 728
DpnII GATC 4 cut(s) 10, 30, 37, 726
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 4 cut(s) 45, 231, 715, 891
EagI CGGCCG 1 cut(s) 891
Eam1104I CTCTTC 3 cut(s) 48, 268, 632
EarI CTCTTC 3 cut(s) 48, 268, 632
EciI GGCGGA 4 cut(s) 55, 209, 511, 910
Ecl136II GAGCTC 1 cut(s) 574
EclXI CGGCCG 1 cut(s) 891
Eco130I CCWWGG 2 cut(s) 48, 630
Eco24I GRGCYC 2 cut(s) 576, 737
Eco32I GATATC 1 cut(s) 682
Eco47I GGWCC 3 cut(s) 201, 227, 304
Eco52I CGGCCG 1 cut(s) 891
Eco53kI GAGCTC 1 cut(s) 574
Eco72I CACGTG 1 cut(s) 799
Eco81I CCTNAGG 1 cut(s) 318
EcoICRI GAGCTC 1 cut(s) 574
EcoRV GATATC 1 cut(s) 682
EcoT14I CCWWGG 2 cut(s) 48, 630
EcoT38I GRGCYC 2 cut(s) 576, 737
EgeI GGCGCC 1 cut(s) 510
EheI GGCGCC 1 cut(s) 510
ErhI CCWWGG 2 cut(s) 48, 630
Esp3I CGTCTC 2 cut(s) 126, 450
FaeI CATG 4 cut(s) 52, 85, 418, 829
FaiI YATR 6 cut(s) 50, 83, 416, 742, 759, 827
FaqI GGGAC 3 cut(s) 290, 872, 918
FatI CATG 4 cut(s) 48, 81, 414, 825
FauI CCCGC 3 cut(s) 221, 543, 876
Fnu4HI GCNGC 6 cut(s) 60, 223, 463, 495, 562, 872
FriOI GRGCYC 2 cut(s) 576, 737
Fsp4HI GCNGC 6 cut(s) 60, 223, 463, 495, 562, 872
FspBI CTAG 2 cut(s) 14, 243
GlaI GCGC 3 cut(s) 510, 806, 808
GluI GCNGC 6 cut(s) 60, 223, 463, 495, 562, 872
HaeII RGCGCY 1 cut(s) 512
HaeIII GGCC 6 cut(s) 47, 59, 233, 515, 717, 893
HhaI GCGC 3 cut(s) 511, 807, 809
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 4 cut(s) 52, 85, 418, 829
Hin6I GCGC 3 cut(s) 509, 805, 807
HinP1I GCGC 3 cut(s) 509, 805, 807
HinfI GANTC 2 cut(s) 248, 906
HphI GGTGA 7 cut(s) 142, 151, 391, 439, 646, 851, 857
Hpy166II GTNNAC 1 cut(s) 420
Hpy188I TCNGA 2 cut(s) 650, 691
Hpy188III TCNNGA 2 cut(s) 193, 812
Hpy8I GTNNAC 1 cut(s) 420
Hpy99I CGWCG 6 cut(s) 38, 167, 430, 433, 572, 695
HpyAV CCTTC 2 cut(s) 526, 696
HpyCH4III ACNGT 3 cut(s) 163, 646, 857
HpyCH4IV ACGT 2 cut(s) 425, 798
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 78, 222, 521
HpyF3I CTNAG 3 cut(s) 318, 490, 883
HpySE526I ACGT 2 cut(s) 425, 798
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 4 cut(s) 52, 85, 418, 829
HspAI GCGC 3 cut(s) 509, 805, 807
KasI GGCGCC 1 cut(s) 508
KroI GCCGGC 3 cut(s) 68, 511, 524
KroNI GCCGGC 3 cut(s) 70, 513, 526
KspI CCGCGG 1 cut(s) 226
Kzo9I GATC 4 cut(s) 10, 30, 37, 726
LmnI GCTCC 3 cut(s) 85, 740, 802
Lsp1109I GCAGC 4 cut(s) 474, 481, 548, 883
LweI GCATC 2 cut(s) 97, 593
MaeI CTAG 2 cut(s) 14, 243
MaeII ACGT 2 cut(s) 425, 798
MaeIII GTNAC 5 cut(s) 206, 322, 445, 835, 857
MalI GATC 4 cut(s) 12, 32, 39, 728
MbiI CCGCTC 1 cut(s) 538
MboI GATC 4 cut(s) 10, 30, 37, 726
MboII GAAGA 5 cut(s) 65, 255, 432, 633, 649
MfeI CAATTG 1 cut(s) 6
MhlI GDGCHC 3 cut(s) 576, 737, 855
MluCI AATT 2 cut(s) 6, 376
Mly113I GGCGCC 1 cut(s) 509
MlyI GAGTC 1 cut(s) 900
MmeI TCCRAC 2 cut(s) 495, 714
MroNI GCCGGC 3 cut(s) 68, 511, 524
MspA1I CMGCKG 2 cut(s) 225, 494
MspR9I CCNGG 3 cut(s) 302, 506, 890
MunI CAATTG 1 cut(s) 6
MvnI CGCG 2 cut(s) 225, 807
MwoI GCNNNNNNNGC 4 cut(s) 65, 78, 222, 521
NaeI GCCGGC 3 cut(s) 70, 513, 526
NarI GGCGCC 1 cut(s) 509
NciI CCSGG 3 cut(s) 302, 506, 890
NcoI CCATGG 1 cut(s) 48
NdeII GATC 4 cut(s) 10, 30, 37, 726
NgoMIV GCCGGC 3 cut(s) 68, 511, 524
NlaIII CATG 4 cut(s) 52, 85, 418, 829
NlaIV GGNNCC 3 cut(s) 306, 510, 736
NmeAIII GCCGAG 1 cut(s) 874
NmuCI GTSAC 3 cut(s) 206, 445, 857
NspV TTCGAA 1 cut(s) 668
PauI GCGCGC 1 cut(s) 805
PcsI WCGNNNNNNNCGW 1 cut(s) 840
PdiI GCCGGC 3 cut(s) 70, 513, 526
PfeI GAWTC 1 cut(s) 248
PflFI GACNNNGTC 1 cut(s) 592
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 6 cut(s) 61, 224, 464, 496, 563, 873
Ple19I CGATCG 1 cut(s) 40
PleI GAGTC 1 cut(s) 900
PluTI GGCGCC 1 cut(s) 512
PmaCI CACGTG 1 cut(s) 799
PmlI CACGTG 1 cut(s) 799
PpsI GAGTC 1 cut(s) 900
Ppu21I YACGTR 1 cut(s) 799
PsiI TTATAA 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 576
PspCI CACGTG 1 cut(s) 799
PspN4I GGNNCC 3 cut(s) 306, 510, 736
PspPI GGNCC 3 cut(s) 201, 227, 304
PsyI GACNNNGTC 1 cut(s) 592
PteI GCGCGC 1 cut(s) 805
PvuI CGATCG 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 2 cut(s) 501, 713
RsaNI GTAC 2 cut(s) 500, 712
Rsr2I CGGWCCG 2 cut(s) 201, 227
RsrII CGGWCCG 2 cut(s) 201, 227
SacI GAGCTC 1 cut(s) 576
SacII CCGCGG 1 cut(s) 226
SatI GCNGC 6 cut(s) 60, 223, 463, 495, 562, 872
Sau3AI GATC 4 cut(s) 10, 30, 37, 726
Sau96I GGNCC 3 cut(s) 201, 227, 304
SchI GAGTC 1 cut(s) 900
ScrFI CCNGG 3 cut(s) 302, 506, 890
SduI GDGCHC 3 cut(s) 576, 737, 855
SfaNI GCATC 2 cut(s) 97, 593
SfoI GGCGCC 1 cut(s) 510
Sfr303I CCGCGG 1 cut(s) 226
SfuI TTCGAA 1 cut(s) 668
SgrBI CCGCGG 1 cut(s) 226
SinI GGWCC 3 cut(s) 201, 227, 304
SmlI CTYRAG 1 cut(s) 810
SmoI CTYRAG 1 cut(s) 810
Sse9I AATT 2 cut(s) 6, 376
SspDI GGCGCC 1 cut(s) 508
SspMI CTAG 2 cut(s) 14, 243
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 3 cut(s) 300, 504, 888
StyI CCWWGG 2 cut(s) 48, 630
TaaI ACNGT 3 cut(s) 163, 646, 857
TaiI ACGT 2 cut(s) 428, 801
TaqI TCGA 6 cut(s) 33, 40, 135, 601, 668, 775
TasI AATT 2 cut(s) 6, 376
TauI GCSGC 2 cut(s) 62, 225
TfiI GAWTC 1 cut(s) 248
TseFI GTSAC 3 cut(s) 206, 445, 857
TseI GCWGC 4 cut(s) 462, 494, 561, 871
Tsp45I GTSAC 3 cut(s) 206, 445, 857
TspDTI ATGAA 1 cut(s) 172
TspGWI ACGGA 2 cut(s) 178, 433
Tth111I GACNNNGTC 1 cut(s) 592
VpaK11BI GGWCC 3 cut(s) 201, 227, 304
XspI CTAG 2 cut(s) 14, 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.