Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002334

Physical Location & Seq

Genomic Coordinates Forward (+)

7467131 .. 7469028

Gene Structure 1898 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1106600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1017 bp

ATGGCGGTGGAGCTGATGAACTTCCCGAAGATGGAGGATCAGAAGGCCATACAGGAAGCGGCATCACAAGGATTGCAGAGCATGGAGCACCTGATCCGGTTCCTCTCTCATCAGCAGCAACAGTCAAACAACCAATCGGCCCGCGTCGACTGTACGGACATCACCGATCACACCGTCTCCAAGTTCAAGAAGGTCATCTCCCTCCTCAATCGGACCGGCCACGCCCGGTTCCGACGCGGTCCGGTTCAGCCGGTTCACTTCCCTACTTCTCACCCCTCGTCTTCACAAACACTCAACTTAGCTTCGGCTCTAAATCTTACTCCGACTCCGGCGCCGGCTCCAGTGACTACTCCGGCTATCGTCCCTTCGACTCCGATCGAGTTGAGTTTCGTCCAATCCCAGCCACACAGCATGACGCTCGACTTCACGAGGCCCAACGCCTTCACGTCGAACCCGAAGAGCGCGGAGATTGAGTTCGCGAAGGAGAGCTTCAGTGTCTCGTCGAGCTCGTCATTCATGTCGTCGGCGATCACCGGAGACGGCAGCGTTTCGAACGGGAAGCTCGGATCGTCGATCTTCTTGGCGCCCGCCCCGGCCGTTTCCGGTGCGAAGCCTCCCCTCTCTACGGCGCCGTATAAGAAGAGGTGCCACGAGCACGACCACTCCGACGACACGTCGTGCAAGTTTTCCGGCTCGGGATCTGCTTCCGGCTCCGGCAAGTGCCATTGCTCCAAGAGAAGGAAAAATCGGGTGAAGAAAACGATTCGTGTGCCGGCGATAAGTTCAAAAATCGCGGATATTCCAGCGGACGAGTACTCCTGGAGAAAGTACGGTCAGAAGCCGATCAAGGGCTCGCCTTACCCAAGGGGATATTACAAGTGCAGTACGGTAAGGGGGTGCCCGGCGAGGAAACACGTGGAGCGCGCTCCGGATGATCCTGCGATGCTGATCGTAACGTACGAAGGGGAGCACCGGCACGCGCCGGAGAACGTGGGGTTGGTGTCTGAGTCGACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Plant_zn_clust WRKY

Protein Analysis

339

Amino Acids

36.7

Weight (kDa)

9.46

Isoelectric Point (pI)

51.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Plant_zn_clust	PF10533	213 - 266	5.9e-20	Plant zinc cluster domain
WRKY	PF03106	270 - 326	6.4e-27	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002334)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24570 AT4G31550 AT4G31550 AT4G31550
fragaria_vesca	FvH4_2g36730 FvH4_2g36730 FvH4_4g06830 FvH4_4g06830
malus_domestica	MD08G1127200.v1.1 MD13G1239100.v1.1 MD15G1106600.v1.1 MD16G1244300.v1.1
prunus_persica	Prupe.1G071400_v2.0.a1 Prupe.1G459100_v2.0.a1
pyrus_communis	pycom13g21150 pycom15g09730 pycom16g20580
rosa_chinensis	RchiOBHm_Chr4g0398741 RchiOBHm_Chr6g0305101
rosa_laevigata	RLG00000009325 RLG00000010919
rosa_multiflora	Rmu_co8094374.1_g000001 Rmu_co8161446.1_g000001 Rmu_co8180642.1_g000001 Rmu_co8389589.1_g000001 Rmu_sc0000061.1_g000025 Rmu_sc0004729.1_g000022 Rmu_sc0011133.1_g000003
rosa_roxburghii	Rroxscaffold_5G00343690 Rroxscaffold_7G00163150
rosa_rugosa	Rorug04G0011200 Rorug06G0337300
rosa_samantha	Rh4AG089100 Rh4BG086100 Rh4CG097100 Rh4DG080700 Rh6AG449100 Rh6BG452000 Rh6CG462800 Rh6DG449500
rosa_wichuraiana	Rw4G007360 Rw6G039120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	5 cut(s)	331, 583, 628, 645, 897
AccI	GTMKAC	2 cut(s)	147, 1010
AccII	CGCG	7 cut(s)	144, 237, 464, 479, 794, 924, 980
AccIII	TCCGGA	1 cut(s)	928
AciI	CCGC	8 cut(s)	5, 59, 142, 237, 464, 588, 794, 806
AclWI	GGATC	5 cut(s)	45, 88, 574, 706, 929
AcoI	YGGCCR	2 cut(s)	217, 594
AcuI	CTGAAG	1 cut(s)	475
AcvI	CACGTG	1 cut(s)	916
AcyI	GRCGYC	3 cut(s)	332, 584, 629
AdeI	CACNNNGTG	1 cut(s)	678
AfaI	GTAC	5 cut(s)	154, 815, 830, 886, 959
AfiI	CCNNNNNNNGG	4 cut(s)	31, 625, 738, 848
AflIII	ACRYGT	2 cut(s)	672, 913
AgsI	TTSAA	2 cut(s)	187, 786
AhdI	GACNNNNNGTC	1 cut(s)	673
AjiI	CACGTC	3 cut(s)	447, 675, 1014
AjnI	CCWGG	1 cut(s)	818
AjuI	GAANNNNNNNTTGG	2 cut(s)	980, 1012
AloI	GAACNNNNNNTCC	2 cut(s)	458, 490
AluBI	AGCT	5 cut(s)	13, 302, 489, 507, 562
AluI	AGCT	5 cut(s)	13, 302, 489, 507, 562
Alw21I	GWGCWC	4 cut(s)	90, 509, 657, 972
Alw26I	GTCTC	3 cut(s)	181, 502, 531
AlwI	GGATC	5 cut(s)	45, 88, 574, 706, 929
Ama87I	CYCGRG	1 cut(s)	694
Aor13HI	TCCGGA	1 cut(s)	928
AoxI	GGCC	5 cut(s)	45, 138, 217, 431, 594
ApeKI	GCWGC	2 cut(s)	115, 543
AspLEI	GCGC	7 cut(s)	334, 464, 586, 631, 924, 926, 982
AspS9I	GGNCC	4 cut(s)	139, 213, 239, 432
AsuC2I	CCSGG	3 cut(s)	226, 593, 902
AsuHPI	GGTGA	4 cut(s)	154, 263, 523, 763
AsuII	TTCGAA	1 cut(s)	551
AvaI	CYCGRG	1 cut(s)	694
AvaII	GGWCC	2 cut(s)	213, 239
BaeGI	GKGCMC	1 cut(s)	902
BanI	GGYRCC	5 cut(s)	331, 583, 628, 645, 897
BanII	GRGCYC	2 cut(s)	509, 854
BauI	CACGAG	2 cut(s)	427, 650
BbrPI	CACGTG	1 cut(s)	916
BbsI	GAAGAC	1 cut(s)	273
Bbv12I	GWGCWC	4 cut(s)	90, 509, 657, 972
BbvI	GCAGC	2 cut(s)	127, 555
BccI	CCATC	1 cut(s)	25
BceAI	ACGGC	4 cut(s)	556, 581, 616, 642
BcgI	CGANNNNNNTGC	4 cut(s)	400, 434, 751, 785
BciT130I	CCWGG	1 cut(s)	820
BcnI	CCSGG	3 cut(s)	226, 593, 902
BcoDI	GTCTC	3 cut(s)	181, 502, 531
BfoI	RGCGCY	3 cut(s)	335, 587, 632
BisI	GCNGC	3 cut(s)	60, 116, 544
BlsI	GCNGC	3 cut(s)	61, 117, 545
BmcAI	AGTACT	1 cut(s)	815
Bme1390I	CCNGG	4 cut(s)	226, 593, 820, 902
Bme18I	GGWCC	2 cut(s)	213, 239
BmeRI	GACNNNNNGTC	1 cut(s)	673
BmeT110I	CYCGRG	1 cut(s)	694
BmgBI	CACGTC	3 cut(s)	447, 675, 1014
BmgT120I	GGNCC	4 cut(s)	139, 213, 239, 432
BmiI	GGNNCC	9 cut(s)	101, 230, 333, 339, 585, 630, 647, 712, 899
BmrFI	CCNGG	4 cut(s)	226, 593, 820, 902
BmsI	GCATC	2 cut(s)	71, 933
BpiI	GAAGAC	1 cut(s)	273
BpmI	CTGGAG	2 cut(s)	324, 841
Bpu14I	TTCGAA	1 cut(s)	551
BpuMI	CCSGG	3 cut(s)	226, 593, 902
BsaAI	YACGTR	1 cut(s)	916
BsaBI	GATNNNNATC	1 cut(s)	947
BsaHI	GRCGYC	3 cut(s)	332, 584, 629
BsaJI	CCNNGG	2 cut(s)	591, 863
BsaWI	WCCGGW	5 cut(s)	96, 241, 533, 602, 928
Bsc4I	CCNNNNNNNGG	4 cut(s)	31, 625, 738, 848
Bse118I	RCCGGY	5 cut(s)	215, 250, 334, 772, 972
Bse1I	ACTGG	1 cut(s)	341
Bse3DI	GCAATG	1 cut(s)	724
Bse8I	GATNNNNATC	1 cut(s)	947
BseAI	TCCGGA	1 cut(s)	928
BseBI	CCWGG	1 cut(s)	820
BseDI	CCNNGG	2 cut(s)	591, 863
BseGI	GGATG	1 cut(s)	937
BseJI	GATNNNNATC	1 cut(s)	947
BseLI	CCNNNNNNNGG	4 cut(s)	31, 625, 738, 848
BseMI	GCAATG	1 cut(s)	724
BseMII	CTCAG	1 cut(s)	996
BseNI	ACTGG	1 cut(s)	341
BsePI	GCGCGC	1 cut(s)	922
BseRI	GAGGAG	1 cut(s)	194
BseSI	GKGCMC	1 cut(s)	902
BseX3I	CGGCCG	1 cut(s)	594
BseXI	GCAGC	2 cut(s)	127, 555
BseYI	CCCAGC	1 cut(s)	399
BsgI	GTGCAG	1 cut(s)	901
Bsh1236I	CGCG	7 cut(s)	144, 237, 464, 479, 794, 924, 980
Bsh1285I	CGRYCG	2 cut(s)	378, 597
BshFI	GGCC	5 cut(s)	47, 140, 219, 433, 596
BshNI	GGYRCC	5 cut(s)	331, 583, 628, 645, 897
BsiEI	CGRYCG	2 cut(s)	378, 597
BsiHKAI	GWGCWC	4 cut(s)	90, 509, 657, 972
BsiHKCI	CYCGRG	1 cut(s)	694
BsiWI	CGTACG	1 cut(s)	957
BslFI	GGGAC	1 cut(s)	347
BslI	CCNNNNNNNGG	4 cut(s)	31, 625, 738, 848
BsmAI	GTCTC	3 cut(s)	181, 502, 531
BsmBI	CGTCTC	2 cut(s)	181, 531
BsmFI	GGGAC	1 cut(s)	347
BsnI	GGCC	5 cut(s)	47, 140, 219, 433, 596
BsoBI	CYCGRG	1 cut(s)	694
Bsp119I	TTCGAA	1 cut(s)	551
Bsp1286I	GDGCHC	6 cut(s)	90, 509, 657, 854, 902, 972
Bsp13I	TCCGGA	1 cut(s)	928
Bsp68I	TCGCGA	1 cut(s)	479
BspACI	CCGC	8 cut(s)	5, 59, 142, 237, 464, 588, 794, 806
BspANI	GGCC	5 cut(s)	47, 140, 219, 433, 596
BspCNI	CTCAG	1 cut(s)	997
BspEI	TCCGGA	1 cut(s)	928
BspFNI	CGCG	7 cut(s)	144, 237, 464, 479, 794, 924, 980
BspLI	GGNNCC	9 cut(s)	101, 230, 333, 339, 585, 630, 647, 712, 899
BspPI	GGATC	5 cut(s)	45, 88, 574, 706, 929
BspQI	GCTCTTC	1 cut(s)	452
BspT104I	TTCGAA	1 cut(s)	551
BspT107I	GGYRCC	5 cut(s)	331, 583, 628, 645, 897
BsrDI	GCAATG	1 cut(s)	724
BsrFI	RCCGGY	5 cut(s)	215, 250, 334, 772, 972
BsrI	ACTGG	1 cut(s)	341
BssAI	RCCGGY	5 cut(s)	215, 250, 334, 772, 972
BssECI	CCNNGG	2 cut(s)	591, 863
BssHII	GCGCGC	1 cut(s)	922
BssNI	GRCGYC	3 cut(s)	332, 584, 629
BssSI	CACGAG	2 cut(s)	427, 650
BssT1I	CCWWGG	1 cut(s)	863
Bst2BI	CACGAG	2 cut(s)	427, 650
Bst2UI	CCWGG	1 cut(s)	820
Bst4CI	ACNGT	5 cut(s)	123, 152, 175, 833, 889
Bst6I	CTCTTC	2 cut(s)	452, 635
BstACI	GRCGYC	3 cut(s)	332, 584, 629
BstBAI	YACGTR	1 cut(s)	916
BstBI	TTCGAA	1 cut(s)	551
BstC8I	GCNNGC	7 cut(s)	142, 336, 588, 774, 854, 924, 978
BstDEI	CTNAG	2 cut(s)	298, 1005
BstF5I	GGATG	1 cut(s)	937
BstFNI	CGCG	7 cut(s)	144, 237, 464, 479, 794, 924, 980
BstH2I	RGCGCY	3 cut(s)	335, 587, 632
BstHHI	GCGC	7 cut(s)	334, 464, 586, 631, 924, 926, 982
BstMAI	GTCTC	3 cut(s)	181, 502, 531
BstMCI	CGRYCG	2 cut(s)	378, 597
BstNI	CCWGG	1 cut(s)	820
BstSCI	CCNGG	4 cut(s)	224, 591, 818, 900
BstSLI	GKGCMC	1 cut(s)	902
BstUI	CGCG	7 cut(s)	144, 237, 464, 479, 794, 924, 980
BstV1I	GCAGC	2 cut(s)	127, 555
BstV2I	GAAGAC	1 cut(s)	273
BstX2I	RGATCY	1 cut(s)	698
BstYI	RGATCY	1 cut(s)	698
BstZI	CGGCCG	1 cut(s)	594
BsuRI	GGCC	5 cut(s)	47, 140, 219, 433, 596
BtgZI	GCGATG	1 cut(s)	956
BtrI	CACGTC	3 cut(s)	447, 675, 1014
BtsCI	GGATG	1 cut(s)	937
BtsIMutI	CAGTG	2 cut(s)	348, 499
BtuMI	TCGCGA	1 cut(s)	479
Cac8I	GCNNGC	7 cut(s)	142, 336, 588, 774, 854, 924, 978
CfoI	GCGC	7 cut(s)	334, 464, 586, 631, 924, 926, 982
Cfr10I	RCCGGY	5 cut(s)	215, 250, 334, 772, 972
Cfr13I	GGNCC	4 cut(s)	139, 213, 239, 432
CpoI	CGGWCCG	2 cut(s)	213, 239
CseI	GACGC	3 cut(s)	133, 243, 424
Csp6I	GTAC	5 cut(s)	153, 814, 829, 885, 958
CspI	CGGWCCG	2 cut(s)	213, 239
CviAII	CATG	3 cut(s)	82, 412, 517
CviQI	GTAC	5 cut(s)	153, 814, 829, 885, 958
DdeI	CTNAG	2 cut(s)	298, 1005
DinI	GGCGCC	3 cut(s)	333, 585, 630
DraIII	CACNNNGTG	1 cut(s)	678
DriI	GACNNNNNGTC	1 cut(s)	673
EaeI	YGGCCR	2 cut(s)	217, 594
EagI	CGGCCG	1 cut(s)	594
Eam1104I	CTCTTC	2 cut(s)	452, 635
Eam1105I	GACNNNNNGTC	1 cut(s)	673
EarI	CTCTTC	2 cut(s)	452, 635
Ecl136II	GAGCTC	1 cut(s)	507
EclXI	CGGCCG	1 cut(s)	594
Eco130I	CCWWGG	1 cut(s)	863
Eco24I	GRGCYC	2 cut(s)	509, 854
Eco47I	GGWCC	2 cut(s)	213, 239
Eco52I	CGGCCG	1 cut(s)	594
Eco53kI	GAGCTC	1 cut(s)	507
Eco57I	CTGAAG	1 cut(s)	475
Eco72I	CACGTG	1 cut(s)	916
Eco88I	CYCGRG	1 cut(s)	694
EcoICRI	GAGCTC	1 cut(s)	507
EcoRII	CCWGG	1 cut(s)	818
EcoT14I	CCWWGG	1 cut(s)	863
EcoT38I	GRGCYC	2 cut(s)	509, 854
EgeI	GGCGCC	3 cut(s)	333, 585, 630
EheI	GGCGCC	3 cut(s)	333, 585, 630
ErhI	CCWWGG	1 cut(s)	863
Esp3I	CGTCTC	2 cut(s)	181, 531
FaeI	CATG	3 cut(s)	85, 415, 520
FaiI	YATR	5 cut(s)	50, 83, 413, 518, 636
FalI	AAGNNNNNCTT	2 cut(s)	473, 505
FaqI	GGGAC	1 cut(s)	347
FatI	CATG	3 cut(s)	81, 411, 516
FauI	CCCGC	2 cut(s)	149, 595
FblI	GTMKAC	2 cut(s)	147, 1010
Fnu4HI	GCNGC	3 cut(s)	60, 116, 544
FokI	GGATG	1 cut(s)	944
FriOI	GRGCYC	2 cut(s)	509, 854
Fsp4HI	GCNGC	3 cut(s)	60, 116, 544
GlaI	GCGC	7 cut(s)	333, 463, 585, 630, 923, 925, 981
GluI	GCNGC	3 cut(s)	60, 116, 544
GsaI	CCCAGC	1 cut(s)	403
GsuI	CTGGAG	2 cut(s)	324, 841
HaeII	RGCGCY	3 cut(s)	335, 587, 632
HaeIII	GGCC	5 cut(s)	47, 140, 219, 433, 596
HgaI	GACGC	3 cut(s)	133, 243, 424
HhaI	GCGC	7 cut(s)	334, 464, 586, 631, 924, 926, 982
Hin1I	GRCGYC	3 cut(s)	332, 584, 629
Hin1II	CATG	3 cut(s)	85, 415, 520
Hin6I	GCGC	7 cut(s)	332, 462, 584, 629, 922, 924, 980
HinP1I	GCGC	7 cut(s)	332, 462, 584, 629, 922, 924, 980
HincII	GTYRAC	2 cut(s)	148, 1011
HindII	GTYRAC	2 cut(s)	148, 1011
HinfI	GANTC	4 cut(s)	325, 370, 763, 1007
HphI	GGTGA	4 cut(s)	154, 263, 523, 763
Hpy166II	GTNNAC	3 cut(s)	148, 256, 1011
Hpy188I	TCNGA	9 cut(s)	42, 213, 233, 324, 375, 566, 667, 837, 1006
Hpy188III	TCNNGA	6 cut(s)	25, 187, 427, 478, 696, 929
Hpy8I	GTNNAC	3 cut(s)	148, 256, 1011
Hpy99I	CGWCG	9 cut(s)	149, 237, 451, 505, 526, 574, 671, 679, 1015
HpyAV	CCTTC	7 cut(s)	37, 184, 375, 451, 475, 732, 956
HpyCH4III	ACNGT	5 cut(s)	123, 152, 175, 833, 889
HpyCH4IV	ACGT	6 cut(s)	446, 674, 915, 956, 990, 1013
HpyCH4V	TGCA	3 cut(s)	76, 681, 882
HpyF3I	CTNAG	2 cut(s)	298, 1005
HpySE526I	ACGT	6 cut(s)	446, 674, 915, 956, 990, 1013
Hsp92I	GRCGYC	3 cut(s)	332, 584, 629
Hsp92II	CATG	3 cut(s)	85, 415, 520
HspAI	GCGC	7 cut(s)	332, 462, 584, 629, 922, 924, 980
KasI	GGCGCC	3 cut(s)	331, 583, 628
Kpn2I	TCCGGA	1 cut(s)	928
KroI	GCCGGC	2 cut(s)	334, 772
KroNI	GCCGGC	2 cut(s)	336, 774
LguI	GCTCTTC	1 cut(s)	452
LmnI	GCTCC	8 cut(s)	10, 85, 343, 716, 734, 919, 931, 967
Lsp1109I	GCAGC	2 cut(s)	127, 555
LweI	GCATC	2 cut(s)	71, 933
MaeII	ACGT	6 cut(s)	446, 674, 915, 956, 990, 1013
MaeIII	GTNAC	2 cut(s)	343, 952
MboII	GAAGA	6 cut(s)	40, 273, 469, 568, 652, 766
MflI	RGATCY	1 cut(s)	698
MhlI	GDGCHC	6 cut(s)	90, 509, 657, 854, 902, 972
Mly113I	GGCGCC	3 cut(s)	332, 584, 629
MlyI	GAGTC	3 cut(s)	319, 364, 1016
MmeI	TCCRAC	3 cut(s)	256, 347, 690
MroI	TCCGGA	1 cut(s)	928
MroNI	GCCGGC	2 cut(s)	334, 772
MspA1I	CMGCKG	1 cut(s)	806
MspR9I	CCNGG	4 cut(s)	226, 593, 820, 902
MvaI	CCWGG	1 cut(s)	820
MvnI	CGCG	7 cut(s)	144, 237, 464, 479, 794, 924, 980
NaeI	GCCGGC	2 cut(s)	336, 774
NarI	GGCGCC	3 cut(s)	332, 584, 629
NciI	CCSGG	3 cut(s)	226, 593, 902
NgoMIV	GCCGGC	2 cut(s)	334, 772
NlaIII	CATG	3 cut(s)	85, 415, 520
NlaIV	GGNNCC	9 cut(s)	101, 230, 333, 339, 585, 630, 647, 712, 899
NmuCI	GTSAC	1 cut(s)	343
NruI	TCGCGA	1 cut(s)	479
NspV	TTCGAA	1 cut(s)	551
PauI	GCGCGC	1 cut(s)	922
PciSI	GCTCTTC	1 cut(s)	452
PcsI	WCGNNNNNNNCGW	4 cut(s)	452, 506, 663, 957
PdiI	GCCGGC	2 cut(s)	336, 774
PfeI	GAWTC	1 cut(s)	763
Pfl23II	CGTACG	1 cut(s)	957
PflFI	GACNNNGTC	1 cut(s)	237
PfoI	TCCNGGA	1 cut(s)	818
PkrI	GCNGC	3 cut(s)	61, 117, 545
Ple19I	CGATCG	1 cut(s)	378
PleI	GAGTC	3 cut(s)	319, 364, 1015
PluTI	GGCGCC	3 cut(s)	335, 587, 632
PmaCI	CACGTG	1 cut(s)	916
PmlI	CACGTG	1 cut(s)	916
PpsI	GAGTC	3 cut(s)	319, 364, 1015
Ppu21I	YACGTR	1 cut(s)	916
Psp124BI	GAGCTC	1 cut(s)	509
Psp6I	CCWGG	1 cut(s)	818
PspCI	CACGTG	1 cut(s)	916
PspFI	CCCAGC	1 cut(s)	399
PspGI	CCWGG	1 cut(s)	818
PspLI	CGTACG	1 cut(s)	957
PspN4I	GGNNCC	9 cut(s)	101, 230, 333, 339, 585, 630, 647, 712, 899
PspPI	GGNCC	4 cut(s)	139, 213, 239, 432
PsuI	RGATCY	1 cut(s)	698
PsyI	GACNNNGTC	1 cut(s)	237
PteI	GCGCGC	1 cut(s)	922
PvuI	CGATCG	1 cut(s)	378
RruI	TCGCGA	1 cut(s)	479
RsaI	GTAC	5 cut(s)	154, 815, 830, 886, 959
RsaNI	GTAC	5 cut(s)	153, 814, 829, 885, 958
Rsr2I	CGGWCCG	2 cut(s)	213, 239
RsrII	CGGWCCG	2 cut(s)	213, 239
SacI	GAGCTC	1 cut(s)	509
SalI	GTCGAC	2 cut(s)	146, 1009
SapI	GCTCTTC	1 cut(s)	452
SatI	GCNGC	3 cut(s)	60, 116, 544
Sau96I	GGNCC	4 cut(s)	139, 213, 239, 432
ScaI	AGTACT	1 cut(s)	815
SchI	GAGTC	3 cut(s)	319, 364, 1016
ScrFI	CCNGG	4 cut(s)	226, 593, 820, 902
SduI	GDGCHC	6 cut(s)	90, 509, 657, 854, 902, 972
SfaNI	GCATC	2 cut(s)	71, 933
SfoI	GGCGCC	3 cut(s)	333, 585, 630
SfuI	TTCGAA	1 cut(s)	551
SinI	GGWCC	2 cut(s)	213, 239
SsiI	CCGC	8 cut(s)	5, 59, 142, 237, 464, 588, 794, 806
SspDI	GGCGCC	3 cut(s)	331, 583, 628
SstI	GAGCTC	1 cut(s)	509
StyD4I	CCNGG	4 cut(s)	224, 591, 818, 900
StyI	CCWWGG	1 cut(s)	863
TaaI	ACNGT	5 cut(s)	123, 152, 175, 833, 889
TaiI	ACGT	6 cut(s)	449, 677, 918, 959, 993, 1016
TaqI	TCGA	9 cut(s)	147, 368, 378, 420, 449, 503, 551, 572, 1010
TatI	WGTACW	1 cut(s)	813
TauI	GCSGC	1 cut(s)	62
TfiI	GAWTC	1 cut(s)	763
TscAI	CASTG	2 cut(s)	348, 499
TseFI	GTSAC	1 cut(s)	343
TseI	GCWGC	2 cut(s)	115, 543
Tsp45I	GTSAC	1 cut(s)	343
TspDTI	ATGAA	2 cut(s)	32, 505
TspGWI	ACGGA	1 cut(s)	170
TspRI	CASTG	2 cut(s)	348, 499
Tth111I	GACNNNGTC	1 cut(s)	237
VpaK11BI	GGWCC	2 cut(s)	213, 239
XmiI	GTMKAC	2 cut(s)	147, 1010
ZrmI	AGTACT	1 cut(s)	815

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.