Rh4CG097100
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002334
Physical Location & Seq
Reverse (-)
17524560 .. 17526843
2284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG097100.1

Sequence Viewer

Length: 744 bp
ATGGCAATTGATCTCGTTAGGTTCTCCAAGATCGACGATCGGACGGCTATGGAAGAGGCCGCCTCCGCCGGCTTACAGAGCATGGAGCATCTCATCCGTGTCTTGTCCAGTCAAACCCCGTCTCACAACAACCTCGACTGCCGTGAAATCACCGACTTCACCGTCGCCAAGTTCAAGCAGTTCATCTCCGTCTTGAACCGGACCGGTCACGCCCGTTTCCGCCGCGGACCGGCTAAGCAACCTAGTGAATCTACCACCACCTCTTCTCCTCCTCCTCCACCTCGTGCCAACCCGGCCCCACCAAAGCCTCAGGTTACTTTGTCTTTTGAAACCGGAAATCCCAATACCAATACCAATACAAACACCCATTCCAAGTCCAAAGACTGCCTCACCTTATCTCCTCCCATGTCCACAACGTCGTCGTTTCTTTCTTCCGTCACCACCGGAGACGGCAGCGTTTCGAACGGCAAAGTTTTCTCCTCAGCTGCCGTCCCCCCGGCGCCGGCCTTCTCCGCCGGTAAACCCCCGCTCTCCCAATCCCACCGGAAACGCTGCCACGACGAGCTCGCAACCAAAACGACATCGTCCAGTCGATGCCATTGTTCTAAAAGAAGAAAATCCAAGGTGAAGAGGACAGTCCGAGTGCCGGCGATTAGTTCGAAAATCGCCGACATACCTTCCGACGAGTTTTCGTGGAGGAAGTACGGCCAAAAGCCGATCAAGGGCTCCCCATACCCACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plant_zn_clust WRKY

Protein Analysis

247

Amino Acids

26.86

Weight (kDa)

10.21

Isoelectric Point (pI)

53.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_zn_clust PF10533 182 - 224 8.8e-17 Plant zinc cluster domain
WRKY PF03106 228 - 247 3.3e-06 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 499
AccBSI CCGCTC 1 cut(s) 529
AccII CGCG 1 cut(s) 225
AciI CCGC 7 cut(s) 60, 66, 220, 223, 225, 513, 527
AcoI YGGCCR 1 cut(s) 706
AcyI GRCGYC 1 cut(s) 500
AdeI CACNNNGTG 1 cut(s) 284
AfaI GTAC 1 cut(s) 704
AfiI CCNNNNNNNGG 4 cut(s) 229, 502, 646, 722
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 3 cut(s) 175, 196, 329
AluBI AGCT 2 cut(s) 485, 565
AluI AGCT 2 cut(s) 485, 565
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 2 cut(s) 126, 441
AoxI GGCC 4 cut(s) 57, 294, 504, 706
ApeKI GCWGC 3 cut(s) 453, 485, 552
AsiGI ACCGGT 1 cut(s) 203
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 3 cut(s) 201, 227, 295
AsuC2I CCSGG 2 cut(s) 293, 497
AsuHPI GGTGA 5 cut(s) 142, 151, 382, 430, 637
AsuII TTCGAA 2 cut(s) 461, 659
AvaII GGWCC 2 cut(s) 201, 227
AxyI CCTNAGG 1 cut(s) 309
BanI GGYRCC 1 cut(s) 499
BanII GRGCYC 2 cut(s) 567, 728
BauI CACGAG 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 567
BbvCI CCTCAGC 1 cut(s) 481
BbvI GCAGC 3 cut(s) 465, 472, 539
BceAI ACGGC 6 cut(s) 60, 126, 466, 473, 481, 721
BcnI CCSGG 2 cut(s) 293, 497
BcoDI GTCTC 2 cut(s) 126, 441
BfaI CTAG 1 cut(s) 243
BfoI RGCGCY 1 cut(s) 503
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 5 cut(s) 60, 223, 454, 486, 553
BlpI GCTNAGC 1 cut(s) 234
BlsI GCNGC 5 cut(s) 61, 224, 455, 487, 554
Bme1390I CCNGG 2 cut(s) 293, 497
Bme18I GGWCC 2 cut(s) 201, 227
BmgT120I GGNCC 3 cut(s) 201, 227, 295
BmiI GGNNCC 3 cut(s) 297, 501, 727
BmrFI CCNGG 2 cut(s) 293, 497
BmsI GCATC 2 cut(s) 97, 584
BplI GAGNNNNNCTC 2 cut(s) 47, 79
Bpu10I CCTNAGC 1 cut(s) 481
Bpu1102I GCTNAGC 1 cut(s) 234
Bpu14I TTCGAA 2 cut(s) 461, 659
BpuMI CCSGG 2 cut(s) 293, 497
BsaHI GRCGYC 1 cut(s) 500
BsaJI CCNNGG 4 cut(s) 223, 495, 621, 737
BsaWI WCCGGW 5 cut(s) 198, 203, 332, 443, 543
BsaXI ACNNNNNCTCC 4 cut(s) 250, 280, 382, 412
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 502, 646, 722
Bse118I RCCGGY 6 cut(s) 68, 203, 229, 502, 515, 646
Bse1I ACTGG 2 cut(s) 108, 588
Bse21I CCTNAGG 1 cut(s) 309
BseDI CCNNGG 4 cut(s) 223, 495, 621, 737
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 4 cut(s) 229, 502, 646, 722
BseMII CTCAG 2 cut(s) 323, 495
BseNI ACTGG 2 cut(s) 108, 588
BseRI GAGGAG 5 cut(s) 258, 261, 264, 390, 469
BseXI GCAGC 3 cut(s) 465, 472, 539
Bsh1236I CGCG 1 cut(s) 225
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 4 cut(s) 59, 296, 506, 708
BshNI GGYRCC 1 cut(s) 499
BshTI ACCGGT 1 cut(s) 203
BsiEI CGRYCG 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 567
BslFI GGGAC 1 cut(s) 476
BslI CCNNNNNNNGG 4 cut(s) 229, 502, 646, 722
BsmAI GTCTC 2 cut(s) 126, 441
BsmBI CGTCTC 2 cut(s) 126, 441
BsmFI GGGAC 1 cut(s) 476
BsnI GGCC 4 cut(s) 59, 296, 506, 708
Bsp119I TTCGAA 2 cut(s) 461, 659
Bsp1286I GDGCHC 2 cut(s) 567, 728
Bsp143I GATC 4 cut(s) 10, 30, 37, 717
Bsp1720I GCTNAGC 1 cut(s) 234
BspACI CCGC 7 cut(s) 60, 66, 220, 223, 225, 513, 527
BspANI GGCC 4 cut(s) 59, 296, 506, 708
BspCNI CTCAG 2 cut(s) 322, 494
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 3 cut(s) 297, 501, 727
BspT104I TTCGAA 2 cut(s) 461, 659
BspT107I GGYRCC 1 cut(s) 499
BsrBI CCGCTC 1 cut(s) 529
BsrFI RCCGGY 6 cut(s) 68, 203, 229, 502, 515, 646
BsrI ACTGG 2 cut(s) 108, 588
BssAI RCCGGY 6 cut(s) 68, 203, 229, 502, 515, 646
BssECI CCNNGG 4 cut(s) 223, 495, 621, 737
BssMI GATC 4 cut(s) 10, 30, 37, 717
BssNI GRCGYC 1 cut(s) 500
BssSI CACGAG 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 621
Bst2BI CACGAG 1 cut(s) 282
Bst4CI ACNGT 3 cut(s) 163, 637, 741
Bst6I CTCTTC 3 cut(s) 48, 268, 623
BstACI GRCGYC 1 cut(s) 500
BstBI TTCGAA 2 cut(s) 461, 659
BstC8I GCNNGC 4 cut(s) 70, 504, 567, 648
BstDEI CTNAG 3 cut(s) 234, 309, 481
BstDSI CCRYGG 2 cut(s) 223, 737
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 1 cut(s) 225
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 4 cut(s) 13, 33, 40, 720
BstMAI GTCTC 2 cut(s) 126, 441
BstMBI GATC 4 cut(s) 10, 30, 37, 717
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 4 cut(s) 65, 78, 293, 512
BstSCI CCNGG 2 cut(s) 291, 495
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 3 cut(s) 465, 472, 539
Bsu36I CCTNAGG 1 cut(s) 309
BsuRI GGCC 4 cut(s) 59, 296, 506, 708
BtgI CCRYGG 2 cut(s) 223, 737
BtsCI GGATG 1 cut(s) 93
Cac8I GCNNGC 4 cut(s) 70, 504, 567, 648
CfoI GCGC 1 cut(s) 502
Cfr10I RCCGGY 6 cut(s) 68, 203, 229, 502, 515, 646
Cfr13I GGNCC 3 cut(s) 201, 227, 295
Cfr42I CCGCGG 1 cut(s) 226
CpoI CGGWCCG 2 cut(s) 201, 227
Csp6I GTAC 1 cut(s) 703
CspAI ACCGGT 1 cut(s) 203
CspI CGGWCCG 2 cut(s) 201, 227
CviAII CATG 2 cut(s) 82, 406
CviQI GTAC 1 cut(s) 703
DdeI CTNAG 3 cut(s) 234, 309, 481
DinI GGCGCC 1 cut(s) 501
DpnI GATC 4 cut(s) 12, 32, 39, 719
DpnII GATC 4 cut(s) 10, 30, 37, 717
DraIII CACNNNGTG 1 cut(s) 284
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 1 cut(s) 706
Eam1104I CTCTTC 3 cut(s) 48, 268, 623
EarI CTCTTC 3 cut(s) 48, 268, 623
EciI GGCGGA 3 cut(s) 55, 209, 502
Ecl136II GAGCTC 1 cut(s) 565
Eco130I CCWWGG 1 cut(s) 621
Eco24I GRGCYC 2 cut(s) 567, 728
Eco47I GGWCC 2 cut(s) 201, 227
Eco53kI GAGCTC 1 cut(s) 565
Eco81I CCTNAGG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 565
EcoT14I CCWWGG 1 cut(s) 621
EcoT38I GRGCYC 2 cut(s) 567, 728
EgeI GGCGCC 1 cut(s) 501
EheI GGCGCC 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 621
Esp3I CGTCTC 2 cut(s) 126, 441
FaeI CATG 2 cut(s) 85, 409
FaiI YATR 5 cut(s) 50, 83, 407, 674, 733
FaqI GGGAC 1 cut(s) 476
FatI CATG 2 cut(s) 81, 405
FauI CCCGC 1 cut(s) 534
Fnu4HI GCNGC 5 cut(s) 60, 223, 454, 486, 553
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 2 cut(s) 567, 728
Fsp4HI GCNGC 5 cut(s) 60, 223, 454, 486, 553
FspBI CTAG 1 cut(s) 243
GlaI GCGC 1 cut(s) 501
GluI GCNGC 5 cut(s) 60, 223, 454, 486, 553
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 4 cut(s) 59, 296, 506, 708
HhaI GCGC 1 cut(s) 502
Hin1I GRCGYC 1 cut(s) 500
Hin1II CATG 2 cut(s) 85, 409
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HinfI GANTC 1 cut(s) 248
HphI GGTGA 5 cut(s) 142, 151, 382, 430, 637
Hpy166II GTNNAC 2 cut(s) 411, 521
Hpy188I TCNGA 3 cut(s) 42, 641, 682
Hpy188III TCNNGA 1 cut(s) 193
Hpy8I GTNNAC 2 cut(s) 411, 521
Hpy99I CGWCG 6 cut(s) 38, 167, 421, 424, 563, 686
HpyAV CCTTC 2 cut(s) 517, 687
HpyCH4III ACNGT 3 cut(s) 163, 637, 741
HpyCH4IV ACGT 1 cut(s) 416
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 78, 293, 512
HpyF3I CTNAG 3 cut(s) 234, 309, 481
HpySE526I ACGT 1 cut(s) 416
Hsp92I GRCGYC 1 cut(s) 500
Hsp92II CATG 2 cut(s) 85, 409
HspAI GCGC 1 cut(s) 500
KasI GGCGCC 1 cut(s) 499
KroI GCCGGC 3 cut(s) 68, 502, 646
KroNI GCCGGC 3 cut(s) 70, 504, 648
KspI CCGCGG 1 cut(s) 226
Kzo9I GATC 4 cut(s) 10, 30, 37, 717
LmnI GCTCC 2 cut(s) 85, 731
Lsp1109I GCAGC 3 cut(s) 465, 472, 539
LweI GCATC 2 cut(s) 97, 584
MaeI CTAG 1 cut(s) 243
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 3 cut(s) 206, 313, 436
MalI GATC 4 cut(s) 12, 32, 39, 719
MbiI CCGCTC 1 cut(s) 529
MboI GATC 4 cut(s) 10, 30, 37, 717
MboII GAAGA 5 cut(s) 65, 255, 423, 624, 640
MfeI CAATTG 1 cut(s) 6
MhlI GDGCHC 2 cut(s) 567, 728
MluCI AATT 1 cut(s) 6
Mly113I GGCGCC 1 cut(s) 500
MmeI TCCRAC 1 cut(s) 705
MroNI GCCGGC 3 cut(s) 68, 502, 646
MspA1I CMGCKG 2 cut(s) 225, 485
MspR9I CCNGG 2 cut(s) 293, 497
MunI CAATTG 1 cut(s) 6
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 4 cut(s) 65, 78, 293, 512
NaeI GCCGGC 3 cut(s) 70, 504, 648
NarI GGCGCC 1 cut(s) 500
NciI CCSGG 2 cut(s) 293, 497
NdeII GATC 4 cut(s) 10, 30, 37, 717
NgoMIV GCCGGC 3 cut(s) 68, 502, 646
NlaIII CATG 2 cut(s) 85, 409
NlaIV GGNNCC 3 cut(s) 297, 501, 727
NmuCI GTSAC 2 cut(s) 206, 436
NspV TTCGAA 2 cut(s) 461, 659
PdiI GCCGGC 3 cut(s) 70, 504, 648
PfeI GAWTC 1 cut(s) 248
PflFI GACNNNGTC 1 cut(s) 583
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 5 cut(s) 61, 224, 455, 487, 554
Ple19I CGATCG 1 cut(s) 40
PluTI GGCGCC 1 cut(s) 503
Psp124BI GAGCTC 1 cut(s) 567
PspN4I GGNNCC 3 cut(s) 297, 501, 727
PspPI GGNCC 3 cut(s) 201, 227, 295
PsyI GACNNNGTC 1 cut(s) 583
PvuI CGATCG 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 1 cut(s) 704
RsaNI GTAC 1 cut(s) 703
Rsr2I CGGWCCG 2 cut(s) 201, 227
RsrII CGGWCCG 2 cut(s) 201, 227
SacI GAGCTC 1 cut(s) 567
SacII CCGCGG 1 cut(s) 226
SatI GCNGC 5 cut(s) 60, 223, 454, 486, 553
Sau3AI GATC 4 cut(s) 10, 30, 37, 717
Sau96I GGNCC 3 cut(s) 201, 227, 295
ScrFI CCNGG 2 cut(s) 293, 497
SduI GDGCHC 2 cut(s) 567, 728
SfaNI GCATC 2 cut(s) 97, 584
SfoI GGCGCC 1 cut(s) 501
Sfr303I CCGCGG 1 cut(s) 226
SfuI TTCGAA 2 cut(s) 461, 659
SgrBI CCGCGG 1 cut(s) 226
SinI GGWCC 2 cut(s) 201, 227
Sse9I AATT 1 cut(s) 6
SsiI CCGC 7 cut(s) 60, 66, 220, 223, 225, 513, 527
SspDI GGCGCC 1 cut(s) 499
SspMI CTAG 1 cut(s) 243
SstI GAGCTC 1 cut(s) 567
StyD4I CCNGG 2 cut(s) 291, 495
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 3 cut(s) 163, 637, 741
TaiI ACGT 1 cut(s) 419
TaqI TCGA 5 cut(s) 33, 135, 461, 592, 659
TasI AATT 1 cut(s) 6
TauI GCSGC 2 cut(s) 62, 225
TfiI GAWTC 1 cut(s) 248
TseFI GTSAC 2 cut(s) 206, 436
TseI GCWGC 3 cut(s) 453, 485, 552
Tsp45I GTSAC 2 cut(s) 206, 436
TspDTI ATGAA 1 cut(s) 172
TspGWI ACGGA 3 cut(s) 86, 178, 424
Tth111I GACNNNGTC 1 cut(s) 583
VpaK11BI GGWCC 2 cut(s) 201, 227
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.