FvH4_4g09350

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001330
Physical Location & Seq
Forward (+)
11068957 .. 11071177
2221 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g09350.t1

Sequence Viewer

Length: 909 bp
ATGTGTGAGGTTTCTGTTGGTGGCACATATTGGGCACAGGGAAAATGGGCACAGGGAAAAGAAGACGGTCGACATGCGACCGTCGATGGGAAAAGGACTATGACAAGTGGAGGTCTTCTTGGTCCTATATATGTATCAAAAATTATACAGCGCACCCGGGTGCGCCGCTCACCCGTCATCTCCACCGTTGATTTTGACGCGGACTTAACAGAGCCTTTTGACTTTTTCAAAATCAACGGTGGAGATGACTGGTGCGCGGCGCACCCGGGTGCGCTGAATAGGCTCTCTGTATGTATCTGGAAACATATTTGGACCTCAACGTCCCCGGGAAGGTCAAAATACATGCATGGAAAGCTTGGGACTCCATCCTTCCCAGTGTTAATACCTCTTGTATTGGCTGGTTGGAAGCCTTGTTGTTCTCGGCTCTCTAAAGATATATTAAGAAGCTTCTATGGGTACGCTATGGCACTTGTGGTCTGCGAGAGCGACTGTCTAAAGGTAGTGAATGATGTCCAGGGTGGCCTTGCTGATCTTTCTGGCTGTGGCACTCTTATCTCTGATATCAAGTCATTGTTGGATCTTTGTCCAGAACCTGAGCTCCCATACTACATGTGTAGACCTGAACTTCATGCTAGTAATCCCGGACTGTGTTGGCACCAGTGGCTAGCCAGAACTGCATCATCATATATATTTGGCGGCAGATATCCTCCGATGAGGTCCCTGAGCGAGCGCATCCACCAAGACCCGAACAGCCGGGCTCGTCGTTCTCACCAACGACGTCCACGTCAGTCAAGTCCAACGAGCTCCTCGTCGGTCAACGTTGTCGCCGCTGTCATGGAAGATGCTTCCCGGAGGCTTAGTACTTTGGTTTTCTTCTTCGGATCCTCCTCCTCAATTTCTGGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

303

Amino Acids

33.31

Weight (kDa)

9.06

Isoelectric Point (pI)

57.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 319, 783
AatII GACGTC 1 cut(s) 781
AccB1I GGYRCC 1 cut(s) 654
AccBSI CCGCTC 1 cut(s) 168
AccI GTMKAC 2 cut(s) 70, 616
AccII CGCG 2 cut(s) 200, 257
AciI CCGC 5 cut(s) 166, 200, 257, 696, 828
AclI AACGTT 1 cut(s) 819
AclWI GGATC 3 cut(s) 585, 876, 889
AcyI GRCGYC 1 cut(s) 778
AfaI GTAC 2 cut(s) 458, 862
AfiI CCNNNNNNNGG 2 cut(s) 87, 330
AflIII ACRYGT 1 cut(s) 609
AgsI TTSAA 1 cut(s) 229
AjiI CACGTC 1 cut(s) 785
AjnI CCWGG 1 cut(s) 513
AleI CACNNNNGTG 2 cut(s) 158, 267
AloI GAACNNNNNNTCC 2 cut(s) 582, 614
AluBI AGCT 4 cut(s) 355, 447, 598, 804
AluI AGCT 4 cut(s) 355, 447, 598, 804
Alw21I GWGCWC 2 cut(s) 600, 806
AlwI GGATC 3 cut(s) 585, 876, 889
AlwNI CAGNNNCTG 1 cut(s) 593
Ama87I CYCGRG 3 cut(s) 156, 265, 325
AoxI GGCC 1 cut(s) 520
AspLEI GCGC 6 cut(s) 153, 165, 257, 262, 274, 732
AspS9I GGNCC 3 cut(s) 122, 312, 717
AsuC2I CCSGG 9 cut(s) 157, 158, 266, 267, 326, 327, 642, 755, 850
AsuHPI GGTGA 2 cut(s) 162, 761
AsuNHI GCTAGC 1 cut(s) 664
AvaI CYCGRG 3 cut(s) 156, 265, 325
AvaII GGWCC 3 cut(s) 122, 312, 717
BaeGI GKGCMC 2 cut(s) 37, 52
BamHI GGATCC 1 cut(s) 881
BanI GGYRCC 1 cut(s) 654
BanII GRGCYC 3 cut(s) 600, 760, 806
BbsI GAAGAC 2 cut(s) 69, 107
Bbv12I GWGCWC 2 cut(s) 600, 806
BccI CCATC 2 cut(s) 80, 373
BciT130I CCWGG 1 cut(s) 515
BcnI CCSGG 9 cut(s) 157, 158, 266, 267, 326, 327, 642, 755, 850
BfaI CTAG 2 cut(s) 633, 665
BisI GCNGC 4 cut(s) 166, 258, 697, 828
BlsI GCNGC 4 cut(s) 167, 259, 698, 829
BmcAI AGTACT 1 cut(s) 862
Bme18I GGWCC 3 cut(s) 122, 312, 717
BmeT110I CYCGRG 3 cut(s) 156, 265, 325
BmgBI CACGTC 1 cut(s) 785
BmgT120I GGNCC 3 cut(s) 122, 312, 717
BmiI GGNNCC 3 cut(s) 656, 719, 883
BmrI ACTGGG 1 cut(s) 368
BmsI GCATC 3 cut(s) 686, 741, 832
BmtI GCTAGC 1 cut(s) 668
BmuI ACTGGG 1 cut(s) 368
BpiI GAAGAC 2 cut(s) 69, 107
Bpu10I CCTNAGC 2 cut(s) 594, 722
BpuMI CCSGG 9 cut(s) 157, 158, 266, 267, 326, 327, 642, 755, 850
BsaHI GRCGYC 1 cut(s) 778
BsaJI CCNNGG 5 cut(s) 156, 265, 324, 325, 514
BsaXI ACNNNNNCTCC 4 cut(s) 582, 612, 844, 874
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 330
Bse1I ACTGG 3 cut(s) 254, 374, 658
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 5 cut(s) 156, 265, 324, 325, 514
BseGI GGATG 2 cut(s) 365, 732
BseLI CCNNNNNNNGG 2 cut(s) 87, 330
BseMII CTCAG 2 cut(s) 585, 713
BseNI ACTGG 3 cut(s) 254, 374, 658
BseRI GAGGAG 3 cut(s) 796, 877, 880
BseSI GKGCMC 2 cut(s) 37, 52
Bsh1236I CGCG 2 cut(s) 200, 257
Bsh1285I CGRYCG 2 cut(s) 70, 81
BshFI GGCC 1 cut(s) 522
BshNI GGYRCC 1 cut(s) 654
BsiEI CGRYCG 2 cut(s) 70, 81
BsiHKAI GWGCWC 2 cut(s) 600, 806
BsiHKCI CYCGRG 3 cut(s) 156, 265, 325
BsiSI CCGG 6 cut(s) 157, 266, 326, 642, 754, 850
BslFI GGGAC 3 cut(s) 307, 373, 703
BslI CCNNNNNNNGG 2 cut(s) 87, 330
BsmFI GGGAC 3 cut(s) 307, 373, 703
BsnI GGCC 1 cut(s) 522
BsoBI CYCGRG 3 cut(s) 156, 265, 325
Bsp1286I GDGCHC 5 cut(s) 37, 52, 600, 760, 806
Bsp143I GATC 3 cut(s) 529, 577, 881
BspACI CCGC 5 cut(s) 166, 200, 257, 696, 828
BspANI GGCC 1 cut(s) 522
BspCNI CTCAG 2 cut(s) 586, 714
BspFNI CGCG 2 cut(s) 200, 257
BspLI GGNNCC 3 cut(s) 656, 719, 883
BspOI GCTAGC 1 cut(s) 668
BspPI GGATC 3 cut(s) 585, 876, 889
BspT107I GGYRCC 1 cut(s) 654
BsrBI CCGCTC 1 cut(s) 168
BsrI ACTGG 3 cut(s) 254, 374, 658
BssECI CCNNGG 5 cut(s) 156, 265, 324, 325, 514
BssMI GATC 3 cut(s) 529, 577, 881
BssNI GRCGYC 1 cut(s) 778
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 6 cut(s) 68, 82, 187, 239, 491, 648
BstACI GRCGYC 1 cut(s) 778
BstC8I GCNNGC 2 cut(s) 666, 728
BstDEI CTNAG 3 cut(s) 594, 722, 857
BstF5I GGATG 2 cut(s) 365, 732
BstFNI CGCG 2 cut(s) 200, 257
BstHHI GCGC 6 cut(s) 153, 165, 257, 262, 274, 732
BstKTI GATC 3 cut(s) 532, 580, 884
BstMBI GATC 3 cut(s) 529, 577, 881
BstMCI CGRYCG 2 cut(s) 70, 81
BstMWI GCNNNNNNNGC 4 cut(s) 280, 352, 661, 674
BstNI CCWGG 1 cut(s) 515
BstNSI RCATGY 3 cut(s) 77, 346, 613
BstSLI GKGCMC 2 cut(s) 37, 52
BstUI CGCG 2 cut(s) 200, 257
BstV2I GAAGAC 2 cut(s) 69, 107
BstX2I RGATCY 2 cut(s) 577, 881
BstYI RGATCY 2 cut(s) 577, 881
BsuRI GGCC 1 cut(s) 522
BtrI CACGTC 1 cut(s) 785
BtsCI GGATG 2 cut(s) 365, 732
BtsIMutI CAGTG 2 cut(s) 381, 665
Cac8I GCNNGC 2 cut(s) 666, 728
CaiI CAGNNNCTG 1 cut(s) 593
CfoI GCGC 6 cut(s) 153, 165, 257, 262, 274, 732
Cfr13I GGNCC 3 cut(s) 122, 312, 717
Cfr9I CCCGGG 3 cut(s) 156, 265, 325
CseI GACGC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 457, 861
CviAII CATG 6 cut(s) 74, 343, 347, 610, 629, 835
CviQI GTAC 2 cut(s) 457, 861
DdeI CTNAG 3 cut(s) 594, 722, 857
DpnI GATC 3 cut(s) 531, 579, 883
DpnII GATC 3 cut(s) 529, 577, 881
DrdI GACNNNNNNGTC 2 cut(s) 319, 783
DseDI GACNNNNNNGTC 2 cut(s) 319, 783
Ecl136II GAGCTC 2 cut(s) 598, 804
Eco24I GRGCYC 3 cut(s) 600, 760, 806
Eco32I GATATC 2 cut(s) 562, 704
Eco47I GGWCC 3 cut(s) 122, 312, 717
Eco53kI GAGCTC 2 cut(s) 598, 804
Eco88I CYCGRG 3 cut(s) 156, 265, 325
EcoICRI GAGCTC 2 cut(s) 598, 804
EcoO109I RGGNCCY 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 513
EcoRV GATATC 2 cut(s) 562, 704
EcoT22I ATGCAT 1 cut(s) 348
EcoT38I GRGCYC 3 cut(s) 600, 760, 806
FaeI CATG 6 cut(s) 77, 346, 350, 613, 632, 838
FaqI GGGAC 3 cut(s) 307, 373, 703
FatI CATG 6 cut(s) 73, 342, 346, 609, 628, 834
FblI GTMKAC 2 cut(s) 70, 616
Fnu4HI GCNGC 4 cut(s) 166, 258, 697, 828
FokI GGATG 2 cut(s) 352, 719
FriOI GRGCYC 3 cut(s) 600, 760, 806
Fsp4HI GCNGC 4 cut(s) 166, 258, 697, 828
FspBI CTAG 2 cut(s) 633, 665
GlaI GCGC 6 cut(s) 152, 164, 256, 261, 273, 731
GluI GCNGC 4 cut(s) 166, 258, 697, 828
HaeIII GGCC 1 cut(s) 522
HapII CCGG 6 cut(s) 157, 266, 326, 642, 754, 850
HgaI GACGC 1 cut(s) 206
HhaI GCGC 6 cut(s) 153, 165, 257, 262, 274, 732
Hin1I GRCGYC 1 cut(s) 778
Hin1II CATG 6 cut(s) 77, 346, 350, 613, 632, 838
Hin6I GCGC 6 cut(s) 151, 163, 255, 260, 272, 730
HinP1I GCGC 6 cut(s) 151, 163, 255, 260, 272, 730
HincII GTYRAC 2 cut(s) 71, 817
HindII GTYRAC 2 cut(s) 71, 817
HindIII AAGCTT 2 cut(s) 353, 445
HinfI GANTC 1 cut(s) 361
HpaII CCGG 6 cut(s) 157, 266, 326, 642, 754, 850
HphI GGTGA 2 cut(s) 162, 761
Hpy166II GTNNAC 4 cut(s) 71, 617, 782, 817
Hpy188I TCNGA 3 cut(s) 559, 711, 881
Hpy188III TCNNGA 2 cut(s) 298, 587
Hpy8I GTNNAC 4 cut(s) 71, 617, 782, 817
Hpy99I CGWCG 4 cut(s) 86, 765, 780, 814
HpyAV CCTTC 2 cut(s) 324, 379
HpyCH4III ACNGT 6 cut(s) 68, 82, 187, 239, 491, 648
HpyCH4IV ACGT 4 cut(s) 320, 778, 784, 819
HpyCH4V TGCA 2 cut(s) 346, 677
HpyF10VI GCNNNNNNNGC 4 cut(s) 280, 352, 661, 674
HpyF3I CTNAG 3 cut(s) 594, 722, 857
HpySE526I ACGT 4 cut(s) 320, 778, 784, 819
Hsp92I GRCGYC 1 cut(s) 778
Hsp92II CATG 6 cut(s) 77, 346, 350, 613, 632, 838
HspAI GCGC 6 cut(s) 151, 163, 255, 260, 272, 730
Kzo9I GATC 3 cut(s) 529, 577, 881
LmnI GCTCC 2 cut(s) 603, 809
LweI GCATC 3 cut(s) 686, 741, 832
MaeI CTAG 2 cut(s) 633, 665
MaeII ACGT 4 cut(s) 320, 778, 784, 819
MalI GATC 3 cut(s) 531, 579, 883
MbiI CCGCTC 1 cut(s) 168
MboI GATC 3 cut(s) 529, 577, 881
MboII GAAGA 5 cut(s) 74, 107, 851, 865, 868
MflI RGATCY 2 cut(s) 577, 881
MhlI GDGCHC 5 cut(s) 37, 52, 600, 760, 806
MluCI AATT 2 cut(s) 141, 894
MlyI GAGTC 1 cut(s) 355
MmeI TCCRAC 3 cut(s) 383, 555, 821
Mph1103I ATGCAT 1 cut(s) 348
MseI TTAA 3 cut(s) 206, 380, 440
MslI CAYNNNNRTG 2 cut(s) 158, 267
MspA1I CMGCKG 1 cut(s) 830
MspI CCGG 6 cut(s) 157, 266, 326, 642, 754, 850
MteI GCGCNGCGC 1 cut(s) 258
MvaI CCWGG 1 cut(s) 515
MvnI CGCG 2 cut(s) 200, 257
MwoI GCNNNNNNNGC 4 cut(s) 280, 352, 661, 674
NciI CCSGG 9 cut(s) 157, 158, 266, 267, 326, 327, 642, 755, 850
NdeII GATC 3 cut(s) 529, 577, 881
NheI GCTAGC 1 cut(s) 664
NlaIII CATG 6 cut(s) 77, 346, 350, 613, 632, 838
NlaIV GGNNCC 3 cut(s) 656, 719, 883
NmeAIII GCCGAG 1 cut(s) 400
NsiI ATGCAT 1 cut(s) 348
NspI RCATGY 3 cut(s) 77, 346, 613
OliI CACNNNNGTG 2 cut(s) 158, 267
PciI ACATGT 1 cut(s) 609
PcsI WCGNNNNNNNCGW 2 cut(s) 781, 806
PfoI TCCNGGA 2 cut(s) 640, 848
PkrI GCNGC 4 cut(s) 167, 259, 698, 829
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
PpuMI RGGWCCY 1 cut(s) 717
PscI ACATGT 1 cut(s) 609
Psp124BI GAGCTC 2 cut(s) 600, 806
Psp1406I AACGTT 1 cut(s) 819
Psp5II RGGWCCY 1 cut(s) 717
Psp6I CCWGG 1 cut(s) 513
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 3 cut(s) 656, 719, 883
PspPI GGNCC 3 cut(s) 122, 312, 717
PspPPI RGGWCCY 1 cut(s) 717
PstNI CAGNNNCTG 1 cut(s) 593
PsuI RGATCY 2 cut(s) 577, 881
RsaI GTAC 2 cut(s) 458, 862
RsaNI GTAC 2 cut(s) 457, 861
RseI CAYNNNNRTG 2 cut(s) 158, 267
SacI GAGCTC 2 cut(s) 600, 806
SalI GTCGAC 1 cut(s) 69
SaqAI TTAA 3 cut(s) 206, 380, 440
SatI GCNGC 4 cut(s) 166, 258, 697, 828
Sau3AI GATC 3 cut(s) 529, 577, 881
Sau96I GGNCC 3 cut(s) 122, 312, 717
ScaI AGTACT 1 cut(s) 862
SchI GAGTC 1 cut(s) 355
SduI GDGCHC 5 cut(s) 37, 52, 600, 760, 806
SfaNI GCATC 3 cut(s) 686, 741, 832
SinI GGWCC 3 cut(s) 122, 312, 717
SmaI CCCGGG 3 cut(s) 158, 267, 327
SmiMI CAYNNNNRTG 2 cut(s) 158, 267
Sse9I AATT 2 cut(s) 141, 894
SsiI CCGC 5 cut(s) 166, 200, 257, 696, 828
SspMI CTAG 2 cut(s) 633, 665
SstI GAGCTC 2 cut(s) 600, 806
TaaI ACNGT 6 cut(s) 68, 82, 187, 239, 491, 648
TaiI ACGT 4 cut(s) 323, 781, 787, 822
TaqI TCGA 2 cut(s) 70, 84
TaqII GACCGA 1 cut(s) 802
TasI AATT 2 cut(s) 141, 894
TatI WGTACW 1 cut(s) 860
TauI GCSGC 4 cut(s) 168, 260, 699, 830
Tru1I TTAA 3 cut(s) 206, 380, 440
Tru9I TTAA 3 cut(s) 206, 380, 440
TscAI CASTG 2 cut(s) 381, 665
TspDTI ATGAA 1 cut(s) 617
TspMI CCCGGG 3 cut(s) 156, 265, 325
TspRI CASTG 2 cut(s) 381, 665
VpaK11BI GGWCC 3 cut(s) 122, 312, 717
XceI RCATGY 3 cut(s) 77, 346, 613
XmaI CCCGGG 3 cut(s) 156, 265, 325
XmiI GTMKAC 2 cut(s) 70, 616
XspI CTAG 2 cut(s) 633, 665
ZraI GACGTC 1 cut(s) 779
ZrmI AGTACT 1 cut(s) 862
Zsp2I ATGCAT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.