Rh1AG362700

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001330
Physical Location & Seq
Forward (+)
59746921 .. 59750329
3409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG362700.1

Sequence Viewer

Length: 549 bp
ATGGCGGGACCCGCGAGGCCTCAGTTTGTTCTGTTCGGATCATCCATAGTCCAGCTCAGCTTCAGCCACGGCGGCTGGGGCTCCGTTCTCTCTGACCTCTACTCTCGCAAAGCAGACATATTGCTGCGTGGTTACTTTGGATGGAATTCACGGCGTGCACTAGAGGTGCTTGATCAAGTTTTTCCCAAGGATGCAGCTGTTCAACCATCTTTGGTGATAGTCTATTTTGGCGGGAATGATTCTATGGGGCCTCATCCATCTGGGCTAGGACCTCATGTACCACTTCCTGAGTATATTGAGAACATGAGGAAAATTGCAAATCATCTCCAGAGCCTTTCAGATTCAACACGCATCGTTTTTCTTAGTTGTCCTCCTGTGAATGAGGCCACAGTTCGTGAAAATAAAAGTCCTTATCTCAGCGAGTTAATAAGAACGAATGAGCTATGCCAACAATATTCCGAAGCTTGTATAAAGCTATGCCAGGAACTGGATATCAAGGTGGTTGATCTTTTTACGGCAATACAGAAAGGAGAAAACTGTTTTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

182

Amino Acids

20.21

Weight (kDa)

6.07

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 175 1e-16 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 178 3e-17 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 487
AccII CGCG 1 cut(s) 14
AciI CCGC 4 cut(s) 5, 12, 72, 231
AclWI GGATC 1 cut(s) 46
AcsI RAATTY 1 cut(s) 145
AcuI CTGAAG 1 cut(s) 46
AdeI CACNNNGTG 1 cut(s) 155
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 1 cut(s) 487
AgsI TTSAA 2 cut(s) 203, 345
AjnI CCWGG 1 cut(s) 480
AluBI AGCT 6 cut(s) 55, 60, 197, 442, 464, 475
AluI AGCT 6 cut(s) 55, 60, 197, 442, 464, 475
Alw21I GWGCWC 1 cut(s) 160
Alw44I GTGCAC 1 cut(s) 156
AlwI GGATC 1 cut(s) 46
AlwNI CAGNNNCTG 1 cut(s) 487
AoxI GGCC 3 cut(s) 17, 248, 384
ApaLI GTGCAC 1 cut(s) 156
ApeKI GCWGC 2 cut(s) 124, 194
ApoI RAATTY 1 cut(s) 145
AspS9I GGNCC 3 cut(s) 8, 248, 269
AsuHPI GGTGA 1 cut(s) 226
AvaII GGWCC 2 cut(s) 8, 269
BaeGI GKGCMC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 2 cut(s) 111, 206
BccI CCATC 3 cut(s) 135, 214, 265
BceAI ACGGC 3 cut(s) 85, 167, 531
BciT130I CCWGG 1 cut(s) 482
BclI TGATCA 1 cut(s) 172
BfaI CTAG 2 cut(s) 161, 266
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 3 cut(s) 73, 125, 195
BlpI GCTNAGC 1 cut(s) 56
BlsI GCNGC 3 cut(s) 74, 126, 196
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 2 cut(s) 8, 269
BmgT120I GGNCC 3 cut(s) 8, 248, 269
BmiI GGNNCC 4 cut(s) 9, 10, 82, 249
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 2 cut(s) 181, 360
BpmI CTGGAG 1 cut(s) 311
Bpu1102I GCTNAGC 1 cut(s) 56
BsaAI YACGTR 1 cut(s) 546
BsaJI CCNNGG 2 cut(s) 67, 186
Bsc4I CCNNNNNNNGG 1 cut(s) 487
Bse1I ACTGG 1 cut(s) 492
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 2 cut(s) 67, 186
BseGI GGATG 4 cut(s) 41, 146, 196, 253
BseLI CCNNNNNNNGG 1 cut(s) 487
BseMII CTCAG 4 cut(s) 35, 70, 279, 430
BseNI ACTGG 1 cut(s) 492
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 2 cut(s) 111, 206
BseYI CCCAGC 1 cut(s) 75
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 3 cut(s) 19, 250, 386
BsiHKAI GWGCWC 1 cut(s) 160
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 1 cut(s) 487
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 3 cut(s) 19, 250, 386
Bsp1286I GDGCHC 2 cut(s) 83, 160
Bsp143I GATC 3 cut(s) 38, 172, 505
Bsp1720I GCTNAGC 1 cut(s) 56
BspACI CCGC 4 cut(s) 5, 12, 72, 231
BspANI GGCC 3 cut(s) 19, 250, 386
BspCNI CTCAG 4 cut(s) 34, 69, 280, 429
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 4 cut(s) 9, 10, 82, 249
BspPI GGATC 1 cut(s) 46
BsrI ACTGG 1 cut(s) 492
BssECI CCNNGG 2 cut(s) 67, 186
BssMI GATC 3 cut(s) 38, 172, 505
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 2 cut(s) 391, 539
BstBAI YACGTR 1 cut(s) 546
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 5 cut(s) 21, 56, 288, 362, 416
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 4 cut(s) 41, 146, 196, 253
BstFNI CGCG 1 cut(s) 14
BstKTI GATC 3 cut(s) 41, 175, 508
BstMBI GATC 3 cut(s) 38, 172, 505
BstMWI GCNNNNNNNGC 3 cut(s) 11, 72, 78
BstNI CCWGG 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 480
BstSLI GKGCMC 1 cut(s) 160
BstSNI TACGTA 1 cut(s) 546
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 111, 206
BsuRI GGCC 3 cut(s) 19, 250, 386
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 4 cut(s) 41, 146, 196, 253
Cac8I GCNNGC 1 cut(s) 156
CaiI CAGNNNCTG 1 cut(s) 487
Cfr13I GGNCC 3 cut(s) 8, 248, 269
Csp6I GTAC 1 cut(s) 278
CviAII CATG 2 cut(s) 275, 304
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 5 cut(s) 21, 56, 288, 362, 416
DpnI GATC 3 cut(s) 40, 174, 507
DpnII GATC 3 cut(s) 38, 172, 505
DraIII CACNNNGTG 1 cut(s) 155
Eco105I TACGTA 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 186
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 83
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 2 cut(s) 8, 269
Eco57I CTGAAG 1 cut(s) 46
EcoO109I RGGNCCY 3 cut(s) 8, 248, 269
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 480
EcoRV GATATC 1 cut(s) 493
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 2 cut(s) 278, 307
FaiI YATR 9 cut(s) 47, 119, 245, 276, 294, 305, 445, 470, 478
FaqI GGGAC 1 cut(s) 21
FatI CATG 2 cut(s) 274, 303
FauI CCCGC 2 cut(s) 19, 224
FbaI TGATCA 1 cut(s) 172
Fnu4HI GCNGC 3 cut(s) 73, 125, 195
FokI GGATG 4 cut(s) 28, 153, 203, 240
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 3 cut(s) 73, 125, 195
FspBI CTAG 2 cut(s) 161, 266
GluI GCNGC 3 cut(s) 73, 125, 195
GsaI CCCAGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 3 cut(s) 19, 250, 386
Hin1II CATG 2 cut(s) 278, 307
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 2 cut(s) 239, 341
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 1 cut(s) 158
Hpy188I TCNGA 4 cut(s) 38, 94, 340, 460
Hpy188III TCNNGA 3 cut(s) 287, 328, 395
Hpy8I GTNNAC 1 cut(s) 158
HpyCH4III ACNGT 2 cut(s) 391, 539
HpyCH4IV ACGT 1 cut(s) 545
HpyCH4V TGCA 3 cut(s) 158, 194, 317
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 72, 78
HpyF3I CTNAG 5 cut(s) 21, 56, 288, 362, 416
HpySE526I ACGT 1 cut(s) 545
Hsp92II CATG 2 cut(s) 278, 307
KflI GGGWCCC 1 cut(s) 8
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 3 cut(s) 38, 172, 505
LmnI GCTCC 1 cut(s) 86
LpnPI CCDG 9 cut(s) 61, 65, 246, 300, 341, 387, 467, 473, 494
Lsp1109I GCAGC 2 cut(s) 111, 206
LweI GCATC 2 cut(s) 181, 360
MaeI CTAG 2 cut(s) 161, 266
MaeII ACGT 1 cut(s) 545
MaeIII GTNAC 1 cut(s) 131
MalI GATC 3 cut(s) 40, 174, 507
MboI GATC 3 cut(s) 38, 172, 505
MhlI GDGCHC 2 cut(s) 83, 160
MluCI AATT 2 cut(s) 145, 312
MnlI CCTC 9 cut(s) 9, 30, 107, 157, 261, 282, 300, 376, 381
MseI TTAA 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 197
MspR9I CCNGG 1 cut(s) 482
MvaI CCWGG 1 cut(s) 482
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 3 cut(s) 11, 72, 78
NdeII GATC 3 cut(s) 38, 172, 505
NlaIII CATG 2 cut(s) 278, 307
NlaIV GGNNCC 4 cut(s) 9, 10, 82, 249
PceI AGGCCT 1 cut(s) 19
PfeI GAWTC 2 cut(s) 239, 341
PflMI CCANNNNNTGG 1 cut(s) 487
PkrI GCNGC 3 cut(s) 74, 126, 196
Ppu21I YACGTR 1 cut(s) 546
PpuMI RGGWCCY 2 cut(s) 8, 269
Psp5II RGGWCCY 2 cut(s) 8, 269
Psp6I CCWGG 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 4 cut(s) 9, 10, 82, 249
PspPI GGNCC 3 cut(s) 8, 248, 269
PspPPI RGGWCCY 2 cut(s) 8, 269
PstNI CAGNNNCTG 1 cut(s) 487
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 3 cut(s) 73, 125, 195
Sau3AI GATC 3 cut(s) 38, 172, 505
Sau96I GGNCC 3 cut(s) 8, 248, 269
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 2 cut(s) 83, 160
SfaNI GCATC 2 cut(s) 181, 360
SinI GGWCC 2 cut(s) 8, 269
SnaBI TACGTA 1 cut(s) 546
Sse9I AATT 2 cut(s) 145, 312
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 4 cut(s) 5, 12, 72, 231
SspI AATATT 1 cut(s) 455
SspMI CTAG 2 cut(s) 161, 266
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 480
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 2 cut(s) 391, 539
TaiI ACGT 1 cut(s) 548
TasI AATT 2 cut(s) 145, 312
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 2 cut(s) 239, 341
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TseI GCWGC 2 cut(s) 124, 194
TspGWI ACGGA 1 cut(s) 73
Van91I CCANNNNNTGG 1 cut(s) 487
VneI GTGCAC 1 cut(s) 156
VpaK11BI GGWCC 2 cut(s) 8, 269
XapI RAATTY 1 cut(s) 145
XspI CTAG 2 cut(s) 161, 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.