Rw3G010160

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001330
Physical Location & Seq
Reverse (-)
9749757 .. 9752380
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G010160.1

Sequence Viewer

Length: 747 bp
ATGGTGGGACCTTCAAGACCTCAGTTTGTGCTGTTTGGCTCCTCCATCGTTCAGTACAGTTTCAGCAATGGAGGCTGGGGTGCCATTCTTGCAGACTTATATTCTCGTAAGGCTGATATACTTCTTCGAGGATACGCTGGTTGGAACTCGAGGCGTGCTGTAAAGGTTTTAGAGCAGGCTTTTCCGAAGGATGCAACTATTCAGCCATCATTGGTGATAGTCTATTTCGGTGGTAATGATTCAATGCGCCCTAATCCACTAGGCCTAGGCCCTCATGTACCTCTTTCTGAATATAGCCTTTCAGAGAAAACTCGCATTATCTTTCTCTCTGCTCCTCCTGTCAACGAGGAACAAATTCGTGAGCATTTAAGTGGCATAATTGGGGAGCTGAATCGAACAAACAAGCTATGCAAAATATATTCAGATGCTTGTTTAGAGCTGTGCAGAGAGATGGATATCAAGGCTGTTGATCTCTTCACTTCAATTCAGAAGAGAAAAGACTGGGAAACTACTTGCTTTACTGATGGGGTGCATTTTTCATCTGAGGGGAGCAAGATTGTGGCGGAGGAGATATTAAAGGTCCTTAAAGAGGCGAACTGGGAAAGCAGCCTGCATTGGACGTCATTGGCAACGGAATTCGGAGAGGATTCCCCTTATGATCCCGTCGGTATTGATGGAAAAACTATGAATGTCTCAGAGGATGGCGTTGCGAGACTTCTGCAGTGGGGAATAAGCAACGGGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

248

Amino Acids

27.57

Weight (kDa)

5.33

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 191 9.4e-18 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 185 1.7e-15 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 623
AccB1I GGYRCC 1 cut(s) 80
AciI CCGC 1 cut(s) 563
AclWI GGATC 1 cut(s) 653
AcsI RAATTY 2 cut(s) 354, 635
AcyI GRCGYC 1 cut(s) 620
AfaI GTAC 2 cut(s) 56, 279
AfiI CCNNNNNNNGG 1 cut(s) 589
AgsI TTSAA 3 cut(s) 15, 243, 483
AluBI AGCT 3 cut(s) 388, 406, 439
AluI AGCT 3 cut(s) 388, 406, 439
Alw26I GTCTC 2 cut(s) 697, 706
AlwI GGATC 1 cut(s) 653
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 2 cut(s) 262, 268
ApeKI GCWGC 1 cut(s) 606
ApoI RAATTY 2 cut(s) 354, 635
Asp700I GAANNNNTTC 1 cut(s) 354
AspA2I CCTAGG 1 cut(s) 265
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 3 cut(s) 8, 269, 580
AsuHPI GGTGA 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 2 cut(s) 8, 580
AvrII CCTAGG 1 cut(s) 265
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BanI GGYRCC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 618
BccI CCATC 6 cut(s) 53, 214, 445, 518, 668, 695
BcgI CGANNNNNNTGC 2 cut(s) 700, 734
BciVI GTATCC 1 cut(s) 125
BcoDI GTCTC 2 cut(s) 697, 706
BfaI CTAG 2 cut(s) 260, 266
BfmI CTRYAG 1 cut(s) 719
BfuI GTATCC 1 cut(s) 125
BisI GCNGC 1 cut(s) 607
BlnI CCTAGG 1 cut(s) 265
BlsI GCNGC 1 cut(s) 608
Bme18I GGWCC 2 cut(s) 8, 580
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 3 cut(s) 8, 269, 580
BmiI GGNNCC 3 cut(s) 9, 40, 82
BmrI ACTGGG 2 cut(s) 511, 607
BmsI GCATC 2 cut(s) 181, 415
BmuI ACTGGG 2 cut(s) 511, 607
BsaBI GATNNNNATC 1 cut(s) 455
BsaHI GRCGYC 1 cut(s) 620
BsaJI CCNNGG 1 cut(s) 265
Bsc4I CCNNNNNNNGG 1 cut(s) 589
Bse1I ACTGG 2 cut(s) 506, 602
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 455
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 2 cut(s) 196, 706
BseJI GATNNNNATC 1 cut(s) 455
BseLI CCNNNNNNNGG 1 cut(s) 589
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 3 cut(s) 35, 534, 708
BseNI ACTGG 2 cut(s) 506, 602
BseRI GAGGAG 3 cut(s) 31, 324, 581
BseXI GCAGC 1 cut(s) 618
BseYI CCCAGC 1 cut(s) 75
BsgI GTGCAG 1 cut(s) 463
BshFI GGCC 2 cut(s) 264, 270
BshNI GGYRCC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 148
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 1 cut(s) 589
BsmAI GTCTC 2 cut(s) 697, 706
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 2 cut(s) 264, 270
BsoBI CYCGRG 1 cut(s) 148
Bsp143I GATC 2 cut(s) 469, 658
BspACI CCGC 1 cut(s) 563
BspANI GGCC 2 cut(s) 264, 270
BspCNI CTCAG 3 cut(s) 34, 535, 707
BspLI GGNNCC 3 cut(s) 9, 40, 82
BspMAI CTGCAG 1 cut(s) 723
BspPI GGATC 1 cut(s) 653
BspT107I GGYRCC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 73
BsrI ACTGG 2 cut(s) 506, 602
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 2 cut(s) 469, 658
BssNI GRCGYC 1 cut(s) 620
BssT1I CCWWGG 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 2 cut(s) 479, 485
BstACI GRCGYC 1 cut(s) 620
BstC8I GCNNGC 3 cut(s) 156, 177, 611
BstDEI CTNAG 3 cut(s) 21, 543, 694
BstENI CCTNNNNNAGG 1 cut(s) 587
BstF5I GGATG 2 cut(s) 196, 706
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 2 cut(s) 472, 661
BstMAI GTCTC 2 cut(s) 697, 706
BstMBI GATC 2 cut(s) 469, 658
BstMWI GCNNNNNNNGC 2 cut(s) 72, 89
BstSFI CTRYAG 1 cut(s) 719
BstV1I GCAGC 1 cut(s) 618
BsuI GTATCC 1 cut(s) 125
BsuRI GGCC 2 cut(s) 264, 270
BtsCI GGATG 2 cut(s) 196, 706
BtsI GCAGTG 1 cut(s) 728
BtsIMutI CAGTG 1 cut(s) 728
Cac8I GCNNGC 3 cut(s) 156, 177, 611
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 3 cut(s) 8, 269, 580
Csp6I GTAC 2 cut(s) 55, 278
CviAII CATG 1 cut(s) 275
CviQI GTAC 2 cut(s) 55, 278
DdeI CTNAG 3 cut(s) 21, 543, 694
DpnI GATC 2 cut(s) 471, 660
DpnII GATC 2 cut(s) 469, 658
Eam1104I CTCTTC 2 cut(s) 479, 485
EarI CTCTTC 2 cut(s) 479, 485
EciI GGCGGA 1 cut(s) 578
Eco130I CCWWGG 1 cut(s) 265
Eco147I AGGCCT 1 cut(s) 264
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 2 cut(s) 8, 580
Eco88I CYCGRG 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 587
EcoO109I RGGNCCY 3 cut(s) 8, 269, 580
EcoRI GAATTC 1 cut(s) 635
EcoRV GATATC 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 1 cut(s) 278
FaiI YATR 9 cut(s) 100, 119, 276, 294, 377, 409, 418, 657, 686
FaqI GGGAC 1 cut(s) 21
FatI CATG 1 cut(s) 274
Fnu4HI GCNGC 1 cut(s) 607
FokI GGATG 2 cut(s) 203, 713
Fsp4HI GCNGC 1 cut(s) 607
FspBI CTAG 2 cut(s) 260, 266
GlaI GCGC 1 cut(s) 248
GluI GCNGC 1 cut(s) 607
GsaI CCCAGC 1 cut(s) 79
HaeIII GGCC 2 cut(s) 264, 270
HhaI GCGC 1 cut(s) 249
Hin1I GRCGYC 1 cut(s) 620
Hin1II CATG 1 cut(s) 278
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 3 cut(s) 239, 391, 647
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 8 cut(s) 186, 289, 304, 424, 489, 544, 641, 697
Hpy188III TCNNGA 2 cut(s) 15, 359
Hpy8I GTNNAC 1 cut(s) 343
Hpy99I CGWCG 1 cut(s) 668
HpyAV CCTTC 2 cut(s) 21, 181
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 1 cut(s) 620
HpyCH4V TGCA 7 cut(s) 92, 194, 411, 444, 532, 613, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 89
HpyF3I CTNAG 3 cut(s) 21, 543, 694
HpySE526I ACGT 1 cut(s) 620
Hsp92I GRCGYC 1 cut(s) 620
Hsp92II CATG 1 cut(s) 278
HspAI GCGC 1 cut(s) 247
Kzo9I GATC 2 cut(s) 469, 658
LmnI GCTCC 4 cut(s) 44, 337, 385, 549
LpnPI CCDG 7 cut(s) 61, 123, 161, 351, 487, 583, 623
Lsp1109I GCAGC 1 cut(s) 618
LweI GCATC 2 cut(s) 181, 415
MaeI CTAG 2 cut(s) 260, 266
MaeII ACGT 1 cut(s) 620
MalI GATC 2 cut(s) 471, 660
MboI GATC 2 cut(s) 469, 658
MboII GAAGA 3 cut(s) 116, 466, 502
MluCI AATT 4 cut(s) 354, 378, 483, 635
MmeI TCCRAC 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 4 cut(s) 368, 575, 585, 745
MslI CAYNNNNRTG 1 cut(s) 369
MwoI GCNNNNNNNGC 2 cut(s) 72, 89
NdeII GATC 2 cut(s) 469, 658
NlaIII CATG 1 cut(s) 278
NlaIV GGNNCC 3 cut(s) 9, 40, 82
PaeR7I CTCGAG 1 cut(s) 148
PceI AGGCCT 1 cut(s) 264
PdmI GAANNNNTTC 1 cut(s) 354
PfeI GAWTC 3 cut(s) 239, 391, 647
PkrI GCNGC 1 cut(s) 608
PpuMI RGGWCCY 2 cut(s) 8, 580
Psp5II RGGWCCY 2 cut(s) 8, 580
PspFI CCCAGC 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 9, 40, 82
PspPI GGNCC 3 cut(s) 8, 269, 580
PspPPI RGGWCCY 2 cut(s) 8, 580
PspXI VCTCGAGB 1 cut(s) 148
PstI CTGCAG 1 cut(s) 723
RsaI GTAC 2 cut(s) 56, 279
RsaNI GTAC 2 cut(s) 55, 278
RseI CAYNNNNRTG 1 cut(s) 369
SaqAI TTAA 4 cut(s) 368, 575, 585, 745
SatI GCNGC 1 cut(s) 607
Sau3AI GATC 2 cut(s) 469, 658
Sau96I GGNCC 3 cut(s) 8, 269, 580
SetI ASST 9 cut(s) 13, 22, 168, 283, 390, 408, 441, 582, 623
SfaNI GCATC 2 cut(s) 181, 415
SfcI CTRYAG 1 cut(s) 719
Sfr274I CTCGAG 1 cut(s) 148
SinI GGWCC 2 cut(s) 8, 580
SlaI CTCGAG 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 369
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 4 cut(s) 354, 378, 483, 635
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 1 cut(s) 563
SspMI CTAG 2 cut(s) 260, 266
StuI AGGCCT 1 cut(s) 264
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 1 cut(s) 623
TaqI TCGA 3 cut(s) 127, 149, 394
TasI AATT 4 cut(s) 354, 378, 483, 635
TatI WGTACW 1 cut(s) 54
TfiI GAWTC 3 cut(s) 239, 391, 647
Tru1I TTAA 4 cut(s) 368, 575, 585, 745
Tru9I TTAA 4 cut(s) 368, 575, 585, 745
TscAI CASTG 1 cut(s) 728
TseI GCWGC 1 cut(s) 606
TspDTI ATGAA 2 cut(s) 528, 701
TspGWI ACGGA 1 cut(s) 647
TspRI CASTG 1 cut(s) 728
VpaK11BI GGWCC 2 cut(s) 8, 580
XagI CCTNNNNNAGG 1 cut(s) 587
XapI RAATTY 2 cut(s) 354, 635
XhoI CTCGAG 1 cut(s) 148
XmaJI CCTAGG 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 2 cut(s) 260, 266
ZraI GACGTC 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.