Rorug01G0354100

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001330
Physical Location & Seq
Forward (+)
47088191 .. 47089080
890 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0354100.1

Sequence Viewer

Length: 606 bp
ATGTTGGAACTCAGACTTCAAGCGTCGGTCGAAATTCTGGTGTTCATGGAGACGATGAAGGTGTATGGCGTCAACTATTGCTCGCGAACCGAGCTCGTCGGTTGCTGGCCAACCGAGCTCTTCGATTGCTCGCCAACCGAGCTCTGCCTCCAAGCCTTGGATCCCAGCTATTTGTCTCTGGTGGCCTTGCGGCTCAGACCTGACGCCTTCAAGCACTACCGCTGCGATCGGCCCATCTCTTTGGGAATTGTCCTCCCAAGCATGGCCAAGATCCTCAACTTTGCCGCAAACGACGACGGCGTCGTCACCATCAGGCTGACGATGGCGATAATAGTGAACAATAGTATTGGCTGTATTGCTAGTGTGGATGACAAGCCTGAAGAAGAAGAAGAAGTAACAGTTATAGAAATGAATGAGCCGGTTTCGATCGAATATCAGTTGAGCTGCTTGAAGCCGTTTACAGAAGCTAGCCCGCTGTCAAACACATTTAGGATTAACTTGTCTCGGCAGCTGCCAGTTGTGGTTGAGTACAAAATTTCAAAGACGAGTTATTTTAGGTTCTATTCGACTCCTATAGTCAAGGACGAGGACGGACGGCGTCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

201

Amino Acids

22.77

Weight (kDa)

4.87

Isoelectric Point (pI)

57.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCNA_N PF00705 40 - 103 8.6e-19 Proliferating cell nuclear antigen, N-terminal domain
PCNA_C PF02747 124 - 191 1.3e-13 Proliferating cell nuclear antigen, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 299, 302
AccB7I CCANNNNNTGG 1 cut(s) 157
AccII CGCG 1 cut(s) 85
AciI CCGC 4 cut(s) 190, 220, 285, 473
AclWI GGATC 3 cut(s) 155, 168, 265
AcoI YGGCCR 2 cut(s) 107, 264
AcsI RAATTY 2 cut(s) 33, 534
AcuI CTGAAG 1 cut(s) 399
AcyI GRCGYC 4 cut(s) 69, 204, 300, 598
AfaI GTAC 1 cut(s) 530
AfiI CCNNNNNNNGG 2 cut(s) 157, 262
AgsI TTSAA 4 cut(s) 20, 211, 451, 540
AluBI AGCT 7 cut(s) 94, 118, 142, 168, 444, 467, 511
AluI AGCT 7 cut(s) 94, 118, 142, 168, 444, 467, 511
Alw21I GWGCWC 3 cut(s) 96, 120, 144
Alw26I GTCTC 3 cut(s) 44, 180, 507
AlwI GGATC 3 cut(s) 155, 168, 265
AoxI GGCC 4 cut(s) 107, 183, 230, 264
ApeKI GCWGC 4 cut(s) 222, 444, 508, 511
ApoI RAATTY 2 cut(s) 33, 534
AspS9I GGNCC 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 298
AsuNHI GCTAGC 1 cut(s) 467
BalI TGGCCA 2 cut(s) 109, 266
BamHI GGATCC 1 cut(s) 160
BanII GRGCYC 3 cut(s) 96, 120, 144
Bbv12I GWGCWC 3 cut(s) 96, 120, 144
BbvI GCAGC 4 cut(s) 209, 431, 498, 520
BccI CCATC 3 cut(s) 242, 316, 317
BceAI ACGGC 2 cut(s) 313, 439
BcoDI GTCTC 3 cut(s) 44, 180, 507
BfaI CTAG 3 cut(s) 360, 468, 604
BfmI CTRYAG 1 cut(s) 573
BisI GCNGC 6 cut(s) 191, 223, 285, 445, 509, 512
BlsI GCNGC 6 cut(s) 192, 224, 286, 446, 510, 513
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 1 cut(s) 162
BmtI GCTAGC 1 cut(s) 471
BsaHI GRCGYC 4 cut(s) 69, 204, 300, 598
BsaJI CCNNGG 1 cut(s) 156
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 262
Bse118I RCCGGY 1 cut(s) 418
Bse1I ACTGG 1 cut(s) 515
BseDI CCNNGG 1 cut(s) 156
BseGI GGATG 1 cut(s) 373
BseLI CCNNNNNNNGG 2 cut(s) 157, 262
BseMII CTCAG 2 cut(s) 25, 208
BseNI ACTGG 1 cut(s) 515
BseXI GCAGC 4 cut(s) 209, 431, 498, 520
BseYI CCCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 85
Bsh1285I CGRYCG 3 cut(s) 30, 229, 429
BshFI GGCC 4 cut(s) 109, 185, 232, 266
BsiEI CGRYCG 3 cut(s) 30, 229, 429
BsiHKAI GWGCWC 3 cut(s) 96, 120, 144
BsiSI CCGG 1 cut(s) 419
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 2 cut(s) 157, 262
BsmAI GTCTC 3 cut(s) 44, 180, 507
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 585
BsnI GGCC 4 cut(s) 109, 185, 232, 266
Bsp1286I GDGCHC 3 cut(s) 96, 120, 144
Bsp143I GATC 4 cut(s) 160, 226, 270, 426
Bsp68I TCGCGA 1 cut(s) 85
BspACI CCGC 4 cut(s) 190, 220, 285, 473
BspANI GGCC 4 cut(s) 109, 185, 232, 266
BspCNI CTCAG 2 cut(s) 24, 207
BspFNI CGCG 1 cut(s) 85
BspLI GGNNCC 1 cut(s) 162
BspOI GCTAGC 1 cut(s) 471
BspPI GGATC 3 cut(s) 155, 168, 265
BspQI GCTCTTC 1 cut(s) 125
BsrFI RCCGGY 1 cut(s) 418
BsrI ACTGG 1 cut(s) 515
BssAI RCCGGY 1 cut(s) 418
BssECI CCNNGG 1 cut(s) 156
BssMI GATC 4 cut(s) 160, 226, 270, 426
BssNI GRCGYC 4 cut(s) 69, 204, 300, 598
BssT1I CCWWGG 1 cut(s) 156
Bst4CI ACNGT 1 cut(s) 400
Bst6I CTCTTC 1 cut(s) 125
BstACI GRCGYC 4 cut(s) 69, 204, 300, 598
BstC8I GCNNGC 5 cut(s) 83, 107, 131, 469, 473
BstDEI CTNAG 2 cut(s) 11, 194
BstF5I GGATG 1 cut(s) 373
BstFNI CGCG 1 cut(s) 85
BstKTI GATC 4 cut(s) 163, 229, 273, 429
BstMAI GTCTC 3 cut(s) 44, 180, 507
BstMBI GATC 4 cut(s) 160, 226, 270, 426
BstMCI CGRYCG 3 cut(s) 30, 229, 429
BstMWI GCNNNNNNNGC 3 cut(s) 91, 115, 139
BstSFI CTRYAG 1 cut(s) 573
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 4 cut(s) 209, 431, 498, 520
BstX2I RGATCY 2 cut(s) 160, 270
BstXI CCANNNNNNTGG 1 cut(s) 241
BstYI RGATCY 2 cut(s) 160, 270
BsuRI GGCC 4 cut(s) 109, 185, 232, 266
BtsCI GGATG 1 cut(s) 373
BtuMI TCGCGA 1 cut(s) 85
Cac8I GCNNGC 5 cut(s) 83, 107, 131, 469, 473
Cfr10I RCCGGY 1 cut(s) 418
Cfr13I GGNCC 1 cut(s) 231
CseI GACGC 5 cut(s) 12, 58, 212, 289, 587
Csp6I GTAC 1 cut(s) 529
CviAII CATG 2 cut(s) 46, 262
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 2 cut(s) 11, 194
DpnI GATC 4 cut(s) 162, 228, 272, 428
DpnII GATC 4 cut(s) 160, 226, 270, 426
DrdI GACNNNNNNGTC 2 cut(s) 299, 302
DseDI GACNNNNNNGTC 2 cut(s) 299, 302
EaeI YGGCCR 2 cut(s) 107, 264
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Ecl136II GAGCTC 3 cut(s) 94, 118, 142
Eco130I CCWWGG 1 cut(s) 156
Eco24I GRGCYC 3 cut(s) 96, 120, 144
Eco53kI GAGCTC 3 cut(s) 94, 118, 142
Eco57I CTGAAG 1 cut(s) 399
EcoICRI GAGCTC 3 cut(s) 94, 118, 142
EcoT14I CCWWGG 1 cut(s) 156
EcoT38I GRGCYC 3 cut(s) 96, 120, 144
ErhI CCWWGG 1 cut(s) 156
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 2 cut(s) 49, 265
FaiI YATR 5 cut(s) 47, 66, 263, 404, 575
FaqI GGGAC 1 cut(s) 585
FatI CATG 2 cut(s) 45, 261
FauI CCCGC 1 cut(s) 480
Fnu4HI GCNGC 6 cut(s) 191, 223, 285, 445, 509, 512
FokI GGATG 1 cut(s) 380
FriOI GRGCYC 3 cut(s) 96, 120, 144
Fsp4HI GCNGC 6 cut(s) 191, 223, 285, 445, 509, 512
FspBI CTAG 3 cut(s) 360, 468, 604
GluI GCNGC 6 cut(s) 191, 223, 285, 445, 509, 512
GsaI CCCAGC 1 cut(s) 168
HaeIII GGCC 4 cut(s) 109, 185, 232, 266
HapII CCGG 1 cut(s) 419
HgaI GACGC 5 cut(s) 12, 58, 212, 289, 587
Hin1I GRCGYC 4 cut(s) 69, 204, 300, 598
Hin1II CATG 2 cut(s) 49, 265
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 1 cut(s) 568
HpaII CCGG 1 cut(s) 419
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 3 cut(s) 73, 337, 459
Hpy188I TCNGA 2 cut(s) 14, 197
Hpy188III TCNNGA 1 cut(s) 84
Hpy8I GTNNAC 3 cut(s) 73, 337, 459
Hpy99I CGWCG 5 cut(s) 28, 101, 296, 299, 305
HpyAV CCTTC 2 cut(s) 52, 217
HpyCH4III ACNGT 1 cut(s) 400
HpyF10VI GCNNNNNNNGC 3 cut(s) 91, 115, 139
HpyF3I CTNAG 2 cut(s) 11, 194
Hsp92I GRCGYC 4 cut(s) 69, 204, 300, 598
Hsp92II CATG 2 cut(s) 49, 265
Kzo9I GATC 4 cut(s) 160, 226, 270, 426
LguI GCTCTTC 1 cut(s) 125
LpnPI CCDG 9 cut(s) 23, 91, 164, 178, 213, 298, 390, 432, 528
Lsp1109I GCAGC 4 cut(s) 209, 431, 498, 520
MaeI CTAG 3 cut(s) 360, 468, 604
MaeIII GTNAC 2 cut(s) 304, 394
MalI GATC 4 cut(s) 162, 228, 272, 428
MboI GATC 4 cut(s) 160, 226, 270, 426
MboII GAAGA 5 cut(s) 112, 392, 395, 398, 401
MflI RGATCY 2 cut(s) 160, 270
MhlI GDGCHC 3 cut(s) 96, 120, 144
MlsI TGGCCA 2 cut(s) 109, 266
MluCI AATT 3 cut(s) 33, 246, 534
MluNI TGGCCA 2 cut(s) 109, 266
MlyI GAGTC 1 cut(s) 562
MnlI CCTC 4 cut(s) 158, 263, 284, 580
Mox20I TGGCCA 2 cut(s) 109, 266
MscI TGGCCA 2 cut(s) 109, 266
MseI TTAA 1 cut(s) 495
Msp20I TGGCCA 2 cut(s) 109, 266
MspA1I CMGCKG 3 cut(s) 222, 475, 511
MspI CCGG 1 cut(s) 419
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 3 cut(s) 91, 115, 139
NdeII GATC 4 cut(s) 160, 226, 270, 426
NheI GCTAGC 1 cut(s) 467
NlaIII CATG 2 cut(s) 49, 265
NlaIV GGNNCC 1 cut(s) 162
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 1 cut(s) 304
NruI TCGCGA 1 cut(s) 85
PciSI GCTCTTC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 300
PflFI GACNNNGTC 2 cut(s) 299, 597
PflMI CCANNNNNTGG 1 cut(s) 157
PkrI GCNGC 6 cut(s) 192, 224, 286, 446, 510, 513
Ple19I CGATCG 2 cut(s) 229, 429
PleI GAGTC 1 cut(s) 562
PpsI GAGTC 1 cut(s) 562
Psp124BI GAGCTC 3 cut(s) 96, 120, 144
PspFI CCCAGC 1 cut(s) 164
PspN4I GGNNCC 1 cut(s) 162
PspPI GGNCC 1 cut(s) 231
PsuI RGATCY 2 cut(s) 160, 270
PsyI GACNNNGTC 2 cut(s) 299, 597
PvuI CGATCG 2 cut(s) 229, 429
PvuII CAGCTG 1 cut(s) 511
RruI TCGCGA 1 cut(s) 85
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
SacI GAGCTC 3 cut(s) 96, 120, 144
SapI GCTCTTC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 6 cut(s) 191, 223, 285, 445, 509, 512
Sau3AI GATC 4 cut(s) 160, 226, 270, 426
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 1 cut(s) 562
SduI GDGCHC 3 cut(s) 96, 120, 144
SfcI CTRYAG 1 cut(s) 573
Sse9I AATT 3 cut(s) 33, 246, 534
SsiI CCGC 4 cut(s) 190, 220, 285, 473
SspMI CTAG 3 cut(s) 360, 468, 604
SstI GAGCTC 3 cut(s) 96, 120, 144
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 1 cut(s) 400
TaqI TCGA 5 cut(s) 30, 123, 425, 429, 566
TaqII GACCGA 1 cut(s) 16
TasI AATT 3 cut(s) 33, 246, 534
TatI WGTACW 1 cut(s) 528
TauI GCSGC 2 cut(s) 193, 287
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 4 cut(s) 222, 444, 508, 511
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 3 cut(s) 34, 71, 425
TspGWI ACGGA 1 cut(s) 606
Tth111I GACNNNGTC 2 cut(s) 299, 597
Van91I CCANNNNNTGG 1 cut(s) 157
XapI RAATTY 2 cut(s) 33, 534
XspI CTAG 3 cut(s) 360, 468, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.