pycom04g13830

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001330
Physical Location & Seq
Forward (+)
16735559 .. 16740720
5162 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g13830.2

Sequence Viewer

Length: 789 bp
ATGGTTGGACCAGTAAGACCTCAGTTTGTGATATTTGGTTCGTCCATCGTCCAGTTCAGTTACAGCCATGGAGGATGGGGTGCCATTCTCGCTGACCTATATGCTCGTAAGGCGGACGTACTGCTGCGAGGATATGCTGGTTGGAACTCGAGGCGTGCTCTGCAGGTTTTGGAGCAAGTTTTTCCTAAGGATGCTACAACTCAGCCGTCTTTGGTGATAGTTTATTTCGGTGGTAATGATTCAATGCATCCTCATCCGTCGGGCCTAGGCGCTCATGTACCACGTAATGAATATGTAGAAAATATGCGGAAGATAGCTAAACATTTAAAGAGCCTTTCAGAAAAAACTCGGGTCATCTTTCTTACTGCTCCTCCTGTCAACGAGGAACAAATACGTGAAAACTTGAGTGATCAAACTTCGCCGCAGAAGCGAACAAACGAGTCCTGCAAAATATATTCGGATGCTTGTTTAGAGGTGTGCCGGGAGTTTGATGTCAAAGGTGTTGATTTGTGGACTTCAATTCAGAAAATAAAAGACTGGTCTACTACTTGCTTTAGGGATGGAATCCATTTTTCATCCGAAGGGAGCAAGATTGTGGCGGAGGAGATACTGAAGGTTCTTAGGGAAGCAGACTGGGAGCCCTGCCTACACTGGAAGTCGTTGCCAACAGAATTTTCGGAGGATTCACCTTATGACCCGCCCAGTGCTGATGGAACCACAACTGTGAACATTGCTGAGCAGAGCGCTGCGGCAAGTCCCATGAAGTGGGGGCTAAGCAATGAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

263

Amino Acids

29.32

Weight (kDa)

5.83

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 154
AccB1I GGYRCC 1 cut(s) 80
AccB7I CCANNNNNTGG 1 cut(s) 765
AccI GTMKAC 1 cut(s) 542
AciI CCGC 6 cut(s) 113, 307, 422, 599, 698, 749
AcsI RAATTY 1 cut(s) 671
AcuI CTGAAG 1 cut(s) 632
AfaI GTAC 2 cut(s) 120, 279
AfeI AGCGCT 1 cut(s) 745
AfiI CCNNNNNNNGG 1 cut(s) 765
AgsI TTSAA 2 cut(s) 243, 519
AjuI GAANNNNNNNTTGG 2 cut(s) 658, 690
AleI CACNNNNGTG 1 cut(s) 722
AluBI AGCT 1 cut(s) 317
AluI AGCT 1 cut(s) 317
Alw21I GWGCWC 1 cut(s) 160
Ama87I CYCGRG 2 cut(s) 148, 348
Aor51HI AGCGCT 1 cut(s) 745
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 2 cut(s) 124, 746
ApoI RAATTY 1 cut(s) 671
AspA2I CCTAGG 1 cut(s) 265
AspLEI GCGC 2 cut(s) 272, 746
AspS9I GGNCC 2 cut(s) 8, 262
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 226, 678
AvaI CYCGRG 2 cut(s) 148, 348
AvaII GGWCC 1 cut(s) 8
AvrII CCTAGG 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 186
BanI GGYRCC 1 cut(s) 80
BanII GRGCYC 1 cut(s) 642
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 2 cut(s) 111, 733
BccI CCATC 4 cut(s) 53, 69, 554, 704
BceAI ACGGC 1 cut(s) 190
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 1 cut(s) 482
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 1 cut(s) 161
BfoI RGCGCY 2 cut(s) 273, 747
BfuAI ACCTGC 1 cut(s) 154
BisI GCNGC 4 cut(s) 125, 422, 747, 750
BlnI CCTAGG 1 cut(s) 265
BlpI GCTNAGC 2 cut(s) 735, 773
BlsI GCNGC 4 cut(s) 126, 423, 748, 751
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 1 cut(s) 8
BmeT110I CYCGRG 2 cut(s) 148, 348
BmgT120I GGNCC 2 cut(s) 8, 262
BmiI GGNNCC 3 cut(s) 82, 639, 715
BmrFI CCNGG 1 cut(s) 482
BmrI ACTGGG 2 cut(s) 643, 696
BmsI GCATC 3 cut(s) 181, 256, 451
BmuI ACTGGG 2 cut(s) 643, 696
BplI GAGNNNNNCTC 2 cut(s) 142, 174
Bpu1102I GCTNAGC 2 cut(s) 735, 773
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 1 cut(s) 482
BsaAI YACGTR 2 cut(s) 284, 395
BsaJI CCNNGG 2 cut(s) 67, 265
BsaXI ACNNNNNCTCC 2 cut(s) 355, 385
Bsc4I CCNNNNNNNGG 1 cut(s) 765
Bse1I ACTGG 6 cut(s) 11, 52, 542, 638, 656, 702
Bse21I CCTNAGG 1 cut(s) 186
Bse3DI GCAATG 2 cut(s) 729, 784
BseDI CCNNGG 2 cut(s) 67, 265
BseGI GGATG 7 cut(s) 80, 196, 247, 253, 466, 565, 575
BseLI CCNNNNNNNGG 1 cut(s) 765
BseMI GCAATG 2 cut(s) 729, 784
BseMII CTCAG 3 cut(s) 35, 215, 726
BseNI ACTGG 6 cut(s) 11, 52, 542, 638, 656, 702
BseRI GAGGAG 2 cut(s) 360, 617
BseXI GCAGC 2 cut(s) 111, 733
BshFI GGCC 1 cut(s) 264
BshNI GGYRCC 1 cut(s) 80
BsiHKAI GWGCWC 1 cut(s) 160
BsiHKCI CYCGRG 2 cut(s) 148, 348
BsiSI CCGG 1 cut(s) 481
BslFI GGGAC 1 cut(s) 741
BslI CCNNNNNNNGG 1 cut(s) 765
BsmFI GGGAC 1 cut(s) 741
BsnI GGCC 1 cut(s) 264
BsoBI CYCGRG 2 cut(s) 148, 348
Bsp1286I GDGCHC 2 cut(s) 160, 642
Bsp143I GATC 1 cut(s) 409
Bsp1720I GCTNAGC 2 cut(s) 735, 773
Bsp19I CCATGG 1 cut(s) 67
BspACI CCGC 6 cut(s) 113, 307, 422, 599, 698, 749
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 3 cut(s) 34, 214, 727
BspLI GGNNCC 3 cut(s) 82, 639, 715
BspMAI CTGCAG 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 80
BsrDI GCAATG 2 cut(s) 729, 784
BsrI ACTGG 6 cut(s) 11, 52, 542, 638, 656, 702
BssECI CCNNGG 2 cut(s) 67, 265
BssMI GATC 1 cut(s) 409
BssT1I CCWWGG 2 cut(s) 67, 265
Bst4CI ACNGT 1 cut(s) 724
BstBAI YACGTR 2 cut(s) 284, 395
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 6 cut(s) 21, 186, 201, 620, 735, 773
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 7 cut(s) 80, 196, 247, 253, 466, 565, 575
BstH2I RGCGCY 2 cut(s) 273, 747
BstHHI GCGC 2 cut(s) 272, 746
BstKTI GATC 1 cut(s) 412
BstMBI GATC 1 cut(s) 409
BstMWI GCNNNNNNNGC 4 cut(s) 89, 110, 160, 427
BstSCI CCNGG 1 cut(s) 480
BstSFI CTRYAG 1 cut(s) 161
BstV1I GCAGC 2 cut(s) 111, 733
Bsu36I CCTNAGG 1 cut(s) 186
BsuRI GGCC 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 7 cut(s) 80, 196, 247, 253, 466, 565, 575
BtsIMutI CAGTG 2 cut(s) 649, 709
BveI ACCTGC 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 156
CfoI GCGC 2 cut(s) 272, 746
Cfr13I GGNCC 2 cut(s) 8, 262
Csp6I GTAC 2 cut(s) 119, 278
CviAII CATG 3 cut(s) 68, 275, 760
CviJI RGCY 7 cut(s) 66, 205, 264, 317, 333, 640, 772
CviKI_1 RGCY 7 cut(s) 66, 205, 264, 317, 333, 640, 772
CviQI GTAC 2 cut(s) 119, 278
DdeI CTNAG 6 cut(s) 21, 186, 201, 620, 735, 773
DpnI GATC 1 cut(s) 411
DpnII GATC 1 cut(s) 409
DraI TTTAAA 1 cut(s) 327
EciI GGCGGA 2 cut(s) 128, 614
Eco130I CCWWGG 2 cut(s) 67, 265
Eco24I GRGCYC 1 cut(s) 642
Eco47I GGWCC 1 cut(s) 8
Eco47III AGCGCT 1 cut(s) 745
Eco57I CTGAAG 1 cut(s) 632
Eco81I CCTNAGG 1 cut(s) 186
Eco88I CYCGRG 2 cut(s) 148, 348
EcoT14I CCWWGG 2 cut(s) 67, 265
EcoT22I ATGCAT 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 642
ErhI CCWWGG 2 cut(s) 67, 265
FaeI CATG 3 cut(s) 71, 278, 763
FaqI GGGAC 1 cut(s) 741
FatI CATG 3 cut(s) 67, 274, 759
FauI CCCGC 1 cut(s) 705
FbaI TGATCA 1 cut(s) 409
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 4 cut(s) 125, 422, 747, 750
FokI GGATG 7 cut(s) 87, 203, 234, 240, 473, 562, 572
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 4 cut(s) 125, 422, 747, 750
FspBI CTAG 1 cut(s) 266
GlaI GCGC 2 cut(s) 271, 745
GluI GCNGC 4 cut(s) 125, 422, 747, 750
HaeII RGCGCY 2 cut(s) 273, 747
HaeIII GGCC 1 cut(s) 264
HapII CCGG 1 cut(s) 481
HhaI GCGC 2 cut(s) 272, 746
Hin1II CATG 3 cut(s) 71, 278, 763
Hin6I GCGC 2 cut(s) 270, 744
HinP1I GCGC 2 cut(s) 270, 744
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 4 cut(s) 239, 440, 564, 683
HpaII CCGG 1 cut(s) 481
HphI GGTGA 2 cut(s) 226, 678
Hpy166II GTNNAC 4 cut(s) 379, 513, 543, 727
Hpy188I TCNGA 5 cut(s) 340, 460, 525, 580, 679
Hpy8I GTNNAC 4 cut(s) 379, 513, 543, 727
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 2 cut(s) 575, 607
HpyCH4III ACNGT 1 cut(s) 724
HpyCH4IV ACGT 3 cut(s) 117, 283, 394
HpyCH4V TGCA 3 cut(s) 163, 247, 447
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 110, 160, 427
HpyF3I CTNAG 6 cut(s) 21, 186, 201, 620, 735, 773
HpySE526I ACGT 3 cut(s) 117, 283, 394
Hsp92II CATG 3 cut(s) 71, 278, 763
HspAI GCGC 2 cut(s) 270, 744
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 1 cut(s) 409
LmnI GCTCC 4 cut(s) 172, 373, 585, 637
Lsp1109I GCAGC 2 cut(s) 111, 733
LweI GCATC 3 cut(s) 181, 256, 451
MaeI CTAG 1 cut(s) 266
MaeII ACGT 3 cut(s) 117, 283, 394
MaeIII GTNAC 1 cut(s) 59
MalI GATC 1 cut(s) 411
MboI GATC 1 cut(s) 409
MboII GAAGA 1 cut(s) 322
MhlI GDGCHC 2 cut(s) 160, 642
MluCI AATT 2 cut(s) 519, 671
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 1 cut(s) 122
Mph1103I ATGCAT 1 cut(s) 249
MseI TTAA 2 cut(s) 326, 787
MslI CAYNNNNRTG 1 cut(s) 722
MspI CCGG 1 cut(s) 481
MspR9I CCNGG 1 cut(s) 482
MwoI GCNNNNNNNGC 4 cut(s) 89, 110, 160, 427
NciI CCSGG 1 cut(s) 482
NcoI CCATGG 1 cut(s) 67
NdeII GATC 1 cut(s) 409
NlaIII CATG 3 cut(s) 71, 278, 763
NlaIV GGNNCC 3 cut(s) 82, 639, 715
NsiI ATGCAT 1 cut(s) 249
OliI CACNNNNGTG 1 cut(s) 722
PaeR7I CTCGAG 1 cut(s) 148
PfeI GAWTC 3 cut(s) 239, 564, 683
PflMI CCANNNNNTGG 1 cut(s) 765
PkrI GCNGC 4 cut(s) 126, 423, 748, 751
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
Ppu21I YACGTR 2 cut(s) 284, 395
PspN4I GGNNCC 3 cut(s) 82, 639, 715
PspPI GGNCC 2 cut(s) 8, 262
PspXI VCTCGAGB 1 cut(s) 148
PstI CTGCAG 1 cut(s) 165
RsaI GTAC 2 cut(s) 120, 279
RsaNI GTAC 2 cut(s) 119, 278
RseI CAYNNNNRTG 1 cut(s) 722
SaqAI TTAA 2 cut(s) 326, 787
SatI GCNGC 4 cut(s) 125, 422, 747, 750
Sau3AI GATC 1 cut(s) 409
Sau96I GGNCC 2 cut(s) 8, 262
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 2 cut(s) 160, 642
SfaNI GCATC 3 cut(s) 181, 256, 451
SfcI CTRYAG 1 cut(s) 161
Sfr274I CTCGAG 1 cut(s) 148
SinI GGWCC 1 cut(s) 8
SlaI CTCGAG 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 722
SmlI CTYRAG 2 cut(s) 148, 403
SmoI CTYRAG 2 cut(s) 148, 403
Sse9I AATT 2 cut(s) 519, 671
SsiI CCGC 6 cut(s) 113, 307, 422, 599, 698, 749
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 480
StyI CCWWGG 2 cut(s) 67, 265
TaaI ACNGT 1 cut(s) 724
TaiI ACGT 3 cut(s) 120, 286, 397
TaqI TCGA 1 cut(s) 149
TasI AATT 2 cut(s) 519, 671
TauI GCSGC 2 cut(s) 424, 752
TfiI GAWTC 3 cut(s) 239, 564, 683
Tru1I TTAA 2 cut(s) 326, 787
Tru9I TTAA 2 cut(s) 326, 787
TscAI CASTG 2 cut(s) 656, 709
TseI GCWGC 2 cut(s) 124, 746
TspDTI ATGAA 3 cut(s) 303, 564, 776
TspGWI ACGGA 1 cut(s) 246
TspRI CASTG 2 cut(s) 656, 709
Van91I CCANNNNNTGG 1 cut(s) 765
VpaK11BI GGWCC 1 cut(s) 8
XapI RAATTY 1 cut(s) 671
XhoI CTCGAG 1 cut(s) 148
XmaJI CCTAGG 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 542
XspI CTAG 1 cut(s) 266
Zsp2I ATGCAT 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.