RLG00000024809

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001330
Physical Location & Seq
Forward (+)
39269057 .. 39270085
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024809

Sequence Viewer

Length: 630 bp
ATGGCTAATATCTACCCTCTCTGTCAATATGCGGATATACTTGTGCGTGGCTACGCTGGTTGGAATTCAAGGAATGGTCTGCAGGTTTTAGAGCAAGTGTTTCCAAAGGATGCAAGTATCCAGCCATCGTTGGTGATAGTCTATTTTGGTGGTAATGATTCAATGCTCCCCGATCCACTAGGCTTAGGCCTTCACGTACCTCTTCCTGAATATGTAGAAAATATGAGGAAGATTGCTACCCATTTGAAGAGCCTTTCGCAGAAAACCCGCCTTATCTTCCTGAGTGCTCCTCCTGTCAACGAGGAACAAATGCGAAAAGCATTTAGTGGAATCATGCCGGAGGATAGTCGAACAAACAAGCTGTTGCAAATGTATTCAGATGCTTGTGTGGAGCTGTGCAGAGAGATGAATATCAAGGCAGTTGATCTCTTTACTTCAATGCAGAAAAGAAACGACTGGTCGACTGTATGCTTTTCGGATGGAATCCATTTTTCATCCGAGGGGAGCAAGATCGTGGTGGAGGAGATACTGAAGGTCCTTGAAGAAGCAGACTGGGAACCCAGCCTGCAATGGAACTCATTGCCGACTGAGTTTGAAGAGGTTGCACCTTATACCGCCGCTCGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

210

Amino Acids

23.64

Weight (kDa)

4.95

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL_2 PF13472 12 - 170 4.5e-19 GDSL-like Lipase/Acylhydrolase family
Lipase_GDSL PF00657 25 - 176 2.1e-14 GDSL-like Lipase/Acylhydrolase
DUF459 PF04311 40 - 178 2.5e-06 Protein of unknown function (DUF459)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 73
AccBSI CCGCTC 1 cut(s) 620
AccI GTMKAC 1 cut(s) 461
AciI CCGC 4 cut(s) 32, 268, 615, 618
AclWI GGATC 1 cut(s) 167
AcsI RAATTY 1 cut(s) 64
AcuI CTGAAG 1 cut(s) 551
AfaI GTAC 1 cut(s) 198
AfiI CCNNNNNNNGG 1 cut(s) 621
AgsI TTSAA 6 cut(s) 69, 162, 247, 438, 542, 596
AluBI AGCT 2 cut(s) 361, 394
AluI AGCT 2 cut(s) 361, 394
Alw21I GWGCWC 1 cut(s) 289
AlwI GGATC 1 cut(s) 167
Ama87I CYCGRG 1 cut(s) 621
AoxI GGCC 1 cut(s) 187
ApoI RAATTY 1 cut(s) 64
AspS9I GGNCC 1 cut(s) 535
AsuHPI GGTGA 1 cut(s) 145
AvaI CYCGRG 1 cut(s) 621
AvaII GGWCC 1 cut(s) 535
Bbv12I GWGCWC 1 cut(s) 289
BccI CCATC 2 cut(s) 133, 473
BciVI GTATCC 1 cut(s) 128
BfaI CTAG 1 cut(s) 179
BfmI CTRYAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 128
BisI GCNGC 1 cut(s) 618
BlsI GCNGC 1 cut(s) 619
Bme18I GGWCC 1 cut(s) 535
BmeT110I CYCGRG 1 cut(s) 621
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 1 cut(s) 558
BmrI ACTGGG 1 cut(s) 562
BmsI GCATC 2 cut(s) 100, 370
BmuI ACTGGG 1 cut(s) 562
BplI GAGNNNNNCTC 2 cut(s) 274, 306
Bpu10I CCTNAGC 1 cut(s) 184
BsaAI YACGTR 1 cut(s) 196
BsaBI GATNNNNATC 1 cut(s) 410
BsaJI CCNNGG 1 cut(s) 498
Bsc4I CCNNNNNNNGG 1 cut(s) 621
Bse1I ACTGG 2 cut(s) 461, 557
Bse3DI GCAATG 2 cut(s) 575, 578
Bse8I GATNNNNATC 1 cut(s) 410
BseDI CCNNGG 1 cut(s) 498
BseGI GGATG 3 cut(s) 115, 484, 494
BseJI GATNNNNATC 1 cut(s) 410
BseLI CCNNNNNNNGG 1 cut(s) 621
BseMI GCAATG 2 cut(s) 575, 578
BseMII CTCAG 2 cut(s) 272, 579
BseNI ACTGG 2 cut(s) 461, 557
BseRI GAGGAG 2 cut(s) 279, 536
BseYI CCCAGC 1 cut(s) 560
BsgI GTGCAG 1 cut(s) 418
BshFI GGCC 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 289
BsiHKCI CYCGRG 1 cut(s) 621
BsiSI CCGG 1 cut(s) 338
BslI CCNNNNNNNGG 1 cut(s) 621
BsnI GGCC 1 cut(s) 189
BsoBI CYCGRG 1 cut(s) 621
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 3 cut(s) 172, 424, 510
BspACI CCGC 4 cut(s) 32, 268, 615, 618
BspANI GGCC 1 cut(s) 189
BspCNI CTCAG 2 cut(s) 273, 580
BspLI GGNNCC 1 cut(s) 558
BspMAI CTGCAG 1 cut(s) 84
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 1 cut(s) 167
BspQI GCTCTTC 1 cut(s) 242
BsrBI CCGCTC 1 cut(s) 620
BsrDI GCAATG 2 cut(s) 575, 578
BsrI ACTGG 2 cut(s) 461, 557
BssECI CCNNGG 1 cut(s) 498
BssMI GATC 3 cut(s) 172, 424, 510
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 3 cut(s) 207, 242, 591
BstBAI YACGTR 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 566
BstDEI CTNAG 3 cut(s) 184, 281, 588
BstF5I GGATG 3 cut(s) 115, 484, 494
BstKTI GATC 3 cut(s) 175, 427, 513
BstMBI GATC 3 cut(s) 172, 424, 510
BstSFI CTRYAG 1 cut(s) 80
BsuI GTATCC 1 cut(s) 128
BsuRI GGCC 1 cut(s) 189
BtsCI GGATG 3 cut(s) 115, 484, 494
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 566
Cfr13I GGNCC 1 cut(s) 535
Csp6I GTAC 1 cut(s) 197
CviAII CATG 1 cut(s) 334
CviJI RGCY 9 cut(s) 5, 51, 124, 183, 189, 252, 361, 394, 564
CviKI_1 RGCY 9 cut(s) 5, 51, 124, 183, 189, 252, 361, 394, 564
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 3 cut(s) 184, 281, 588
DpnI GATC 3 cut(s) 174, 426, 512
DpnII GATC 3 cut(s) 172, 424, 510
Eam1104I CTCTTC 3 cut(s) 207, 242, 591
EarI CTCTTC 3 cut(s) 207, 242, 591
Eco147I AGGCCT 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 535
Eco57I CTGAAG 1 cut(s) 551
Eco88I CYCGRG 1 cut(s) 621
EcoO109I RGGNCCY 1 cut(s) 535
EcoRI GAATTC 1 cut(s) 64
FaeI CATG 1 cut(s) 337
FaiI YATR 7 cut(s) 30, 38, 213, 224, 335, 469, 612
FatI CATG 1 cut(s) 333
FauI CCCGC 1 cut(s) 275
FblI GTMKAC 1 cut(s) 461
Fnu4HI GCNGC 1 cut(s) 618
FokI GGATG 3 cut(s) 122, 481, 491
Fsp4HI GCNGC 1 cut(s) 618
FspBI CTAG 1 cut(s) 179
GluI GCNGC 1 cut(s) 618
GsaI CCCAGC 1 cut(s) 564
HaeIII GGCC 1 cut(s) 189
HapII CCGG 1 cut(s) 338
Hin1II CATG 1 cut(s) 337
HincII GTYRAC 2 cut(s) 298, 462
HindII GTYRAC 2 cut(s) 298, 462
HinfI GANTC 3 cut(s) 158, 330, 483
HpaII CCGG 1 cut(s) 338
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 2 cut(s) 298, 462
Hpy188I TCNGA 3 cut(s) 379, 478, 499
Hpy188III TCNNGA 2 cut(s) 206, 280
Hpy8I GTNNAC 2 cut(s) 298, 462
HpyAV CCTTC 2 cut(s) 200, 526
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 7 cut(s) 82, 113, 367, 399, 442, 568, 605
HpyF3I CTNAG 3 cut(s) 184, 281, 588
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 1 cut(s) 337
Kzo9I GATC 3 cut(s) 172, 424, 510
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 4 cut(s) 171, 292, 391, 504
LweI GCATC 2 cut(s) 100, 370
MaeI CTAG 1 cut(s) 179
MaeII ACGT 1 cut(s) 195
MalI GATC 3 cut(s) 174, 426, 512
MbiI CCGCTC 1 cut(s) 620
MboI GATC 3 cut(s) 172, 424, 510
MboII GAAGA 6 cut(s) 194, 241, 259, 268, 554, 608
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 1 cut(s) 64
MmeI TCCRAC 1 cut(s) 41
MnlI CCTC 9 cut(s) 27, 210, 219, 295, 300, 334, 493, 514, 592
MspI CCGG 1 cut(s) 338
NdeII GATC 3 cut(s) 172, 424, 510
NlaIII CATG 1 cut(s) 337
NlaIV GGNNCC 1 cut(s) 558
PceI AGGCCT 1 cut(s) 189
PciSI GCTCTTC 1 cut(s) 242
PfeI GAWTC 3 cut(s) 158, 330, 483
PkrI GCNGC 1 cut(s) 619
Ppu21I YACGTR 1 cut(s) 196
PpuMI RGGWCCY 1 cut(s) 535
Psp5II RGGWCCY 1 cut(s) 535
PspFI CCCAGC 1 cut(s) 560
PspN4I GGNNCC 1 cut(s) 558
PspPI GGNCC 1 cut(s) 535
PspPPI RGGWCCY 1 cut(s) 535
PstI CTGCAG 1 cut(s) 84
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
SalI GTCGAC 1 cut(s) 460
SapI GCTCTTC 1 cut(s) 242
SatI GCNGC 1 cut(s) 618
Sau3AI GATC 3 cut(s) 172, 424, 510
Sau96I GGNCC 1 cut(s) 535
SduI GDGCHC 1 cut(s) 289
SetI ASST 8 cut(s) 87, 198, 202, 363, 396, 537, 603, 610
SfaNI GCATC 2 cut(s) 100, 370
SfcI CTRYAG 1 cut(s) 80
SinI GGWCC 1 cut(s) 535
Sse9I AATT 1 cut(s) 64
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 4 cut(s) 32, 268, 615, 618
SspMI CTAG 1 cut(s) 179
StuI AGGCCT 1 cut(s) 189
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 1 cut(s) 198
TaqI TCGA 2 cut(s) 349, 461
TasI AATT 1 cut(s) 64
TauI GCSGC 1 cut(s) 620
TfiI GAWTC 3 cut(s) 158, 330, 483
TspDTI ATGAA 2 cut(s) 422, 483
VpaK11BI GGWCC 1 cut(s) 535
XapI RAATTY 1 cut(s) 64
XmiI GTMKAC 1 cut(s) 461
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.