RchiOBHm_Chr3g0463151

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001330
Physical Location & Seq
Reverse (-)
10633588 .. 10636087
2500 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42947

Sequence Viewer

Length: 708 bp
ATGGTCGGACCAATAAGACCTCAGTTTGTGCTGTTTGGTTCGTCCATCGTCCAGTTCAGTTTCAGCAATGGAGGTTGGGGCGCCATCCTTGCTGACATATATTCTCGTAAGGCGGATATACTTGTGCGTGGATACGCTGGTTGGAATTCAAGGAATGGTCTGCAGGCTTTAGAGCAAGTGTTTCCAAAGGATGCAAGTATCCAGCCATCGTTGGTGATAGTCTATTTTGGTGGTAATGATTCAATGCTGCCGGATCCACTAGGCTTAAGCCTTCACGTACCTCTTCCTGAATATGTAGAAAATATGAGGAAGATTGCTACCCATTTGAAGAGCCTTTCGCAGAAAACCCGCCTTATCTTCCTGACTGCTCCTCCTGTCAACGAGGAACAAATGCGAAAAGCATTTAGTGGAATCATGCGGGAGAATAGTCGAACAAACAAGCAGTTGCAAATGTATTCAGATGCTTGTGTGGAGCTGTGCAGAGAGATGGATATCAAGGCAGTTGATCTCTTTACTTCAATGCAGAAAAGAAGTGACTGGTCGACTGTATGCTTTACGGATGGAATCCATTTTTCGTCCGAGGGGAGCAAGATCGTGGTGGAGGAGATACTGAAGGTCCTTGAAGAAGCAGACTGGGAACGCCTGCAATGGAACTCATTGCCAACTGAGTTTGAAGAGTTTGCGCCCTATACCGCCTCTCGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

235

Amino Acids

26.58

Weight (kDa)

5.5

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 203 3.5e-21 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 197 3.8e-22 GDSL-like Lipase/Acylhydrolase family
DUF459 PF04311 67 - 205 3.6e-07 Protein of unknown function (DUF459)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 80
AccI GTMKAC 1 cut(s) 542
AciI CCGC 4 cut(s) 113, 349, 418, 693
AclWI GGATC 2 cut(s) 248, 261
AcsI RAATTY 1 cut(s) 145
AcuI CTGAAG 1 cut(s) 632
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 1 cut(s) 699
AflII CTTAAG 1 cut(s) 265
AgsI TTSAA 6 cut(s) 150, 243, 328, 519, 623, 674
AluBI AGCT 1 cut(s) 475
AluI AGCT 1 cut(s) 475
AlwI GGATC 2 cut(s) 248, 261
Ama87I CYCGRG 1 cut(s) 699
ApeKI GCWGC 1 cut(s) 247
ApoI RAATTY 1 cut(s) 145
AspLEI GCGC 2 cut(s) 83, 685
AspS9I GGNCC 2 cut(s) 8, 616
AsuHPI GGTGA 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 699
AvaII GGWCC 2 cut(s) 8, 616
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 234
BccI CCATC 5 cut(s) 53, 92, 214, 481, 554
BciVI GTATCC 2 cut(s) 125, 209
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 161
BfoI RGCGCY 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 265
BfuI GTATCC 2 cut(s) 125, 209
BisI GCNGC 1 cut(s) 248
BlsI GCNGC 1 cut(s) 249
Bme18I GGWCC 2 cut(s) 8, 616
BmeT110I CYCGRG 1 cut(s) 699
BmgT120I GGNCC 2 cut(s) 8, 616
BmiI GGNNCC 2 cut(s) 82, 255
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 2 cut(s) 181, 451
BmuI ACTGGG 1 cut(s) 643
BsaAI YACGTR 1 cut(s) 277
BsaBI GATNNNNATC 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 579
BsaXI ACNNNNNCTCC 2 cut(s) 355, 385
Bsc4I CCNNNNNNNGG 1 cut(s) 699
Bse1I ACTGG 3 cut(s) 52, 542, 638
Bse3DI GCAATG 3 cut(s) 73, 653, 656
Bse8I GATNNNNATC 1 cut(s) 491
BseDI CCNNGG 1 cut(s) 579
BseGI GGATG 3 cut(s) 84, 196, 565
BseJI GATNNNNATC 1 cut(s) 491
BseLI CCNNNNNNNGG 1 cut(s) 699
BseMI GCAATG 3 cut(s) 73, 653, 656
BseMII CTCAG 2 cut(s) 35, 657
BseNI ACTGG 3 cut(s) 52, 542, 638
BseRI GAGGAG 2 cut(s) 360, 617
BseXI GCAGC 1 cut(s) 234
BsgI GTGCAG 1 cut(s) 499
BshNI GGYRCC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 699
BsiSI CCGG 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 699
BsoBI CYCGRG 1 cut(s) 699
Bsp143I GATC 3 cut(s) 253, 505, 591
BspACI CCGC 4 cut(s) 113, 349, 418, 693
BspCNI CTCAG 2 cut(s) 34, 658
BspLI GGNNCC 2 cut(s) 82, 255
BspMAI CTGCAG 1 cut(s) 165
BspPI GGATC 2 cut(s) 248, 261
BspQI GCTCTTC 1 cut(s) 323
BspT107I GGYRCC 1 cut(s) 80
BspTI CTTAAG 1 cut(s) 265
BsrDI GCAATG 3 cut(s) 73, 653, 656
BsrI ACTGG 3 cut(s) 52, 542, 638
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 3 cut(s) 253, 505, 591
BssNI GRCGYC 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 547
Bst6I CTCTTC 3 cut(s) 288, 323, 669
BstACI GRCGYC 1 cut(s) 81
BstAFI CTTAAG 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 277
BstC8I GCNNGC 2 cut(s) 165, 644
BstDEI CTNAG 2 cut(s) 21, 666
BstF5I GGATG 3 cut(s) 84, 196, 565
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 2 cut(s) 83, 685
BstKTI GATC 3 cut(s) 256, 508, 594
BstMBI GATC 3 cut(s) 253, 505, 591
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstSFI CTRYAG 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 234
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuI GTATCC 2 cut(s) 125, 209
BtsCI GGATG 3 cut(s) 84, 196, 565
Cac8I GCNNGC 2 cut(s) 165, 644
CfoI GCGC 2 cut(s) 83, 685
Cfr13I GGNCC 2 cut(s) 8, 616
Csp6I GTAC 1 cut(s) 278
CviAII CATG 1 cut(s) 415
CviJI RGCY 6 cut(s) 167, 205, 264, 270, 333, 475
CviKI_1 RGCY 6 cut(s) 167, 205, 264, 270, 333, 475
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 2 cut(s) 21, 666
DinI GGCGCC 1 cut(s) 82
DpnI GATC 3 cut(s) 255, 507, 593
DpnII GATC 3 cut(s) 253, 505, 591
Eam1104I CTCTTC 3 cut(s) 288, 323, 669
EarI CTCTTC 3 cut(s) 288, 323, 669
EciI GGCGGA 1 cut(s) 128
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 2 cut(s) 8, 616
Eco57I CTGAAG 1 cut(s) 632
Eco88I CYCGRG 1 cut(s) 699
EcoO109I RGGNCCY 1 cut(s) 616
EcoRI GAATTC 1 cut(s) 145
EcoRV GATATC 1 cut(s) 493
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
FaeI CATG 1 cut(s) 418
FaiI YATR 8 cut(s) 98, 100, 119, 294, 305, 416, 550, 690
FatI CATG 1 cut(s) 414
FauI CCCGC 2 cut(s) 356, 411
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 1 cut(s) 248
FokI GGATG 3 cut(s) 71, 203, 572
Fsp4HI GCNGC 1 cut(s) 248
FspBI CTAG 1 cut(s) 260
GlaI GCGC 2 cut(s) 82, 684
GluI GCNGC 1 cut(s) 248
HaeII RGCGCY 1 cut(s) 84
HapII CCGG 1 cut(s) 251
HhaI GCGC 2 cut(s) 83, 685
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 1 cut(s) 418
Hin6I GCGC 2 cut(s) 81, 683
HinP1I GCGC 2 cut(s) 81, 683
HincII GTYRAC 2 cut(s) 379, 543
HindII GTYRAC 2 cut(s) 379, 543
HinfI GANTC 3 cut(s) 239, 411, 564
HpaII CCGG 1 cut(s) 251
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 2 cut(s) 379, 543
Hpy188I TCNGA 3 cut(s) 8, 460, 580
Hpy188III TCNNGA 2 cut(s) 287, 361
Hpy8I GTNNAC 2 cut(s) 379, 543
HpyAV CCTTC 2 cut(s) 281, 607
HpyCH4III ACNGT 1 cut(s) 547
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 6 cut(s) 163, 194, 448, 480, 523, 646
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 2 cut(s) 21, 666
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 1 cut(s) 418
HspAI GCGC 2 cut(s) 81, 683
KasI GGCGCC 1 cut(s) 80
Kzo9I GATC 3 cut(s) 253, 505, 591
LguI GCTCTTC 1 cut(s) 323
LmnI GCTCC 3 cut(s) 373, 472, 585
Lsp1109I GCAGC 1 cut(s) 234
LweI GCATC 2 cut(s) 181, 451
MaeI CTAG 1 cut(s) 260
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 533
MalI GATC 3 cut(s) 255, 507, 593
MboI GATC 3 cut(s) 253, 505, 591
MboII GAAGA 6 cut(s) 275, 322, 340, 349, 635, 686
MflI RGATCY 1 cut(s) 253
MluCI AATT 1 cut(s) 145
Mly113I GGCGCC 1 cut(s) 81
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 9 cut(s) 30, 65, 291, 300, 376, 381, 574, 595, 706
MseI TTAA 1 cut(s) 266
MspCI CTTAAG 1 cut(s) 265
MspI CCGG 1 cut(s) 251
MwoI GCNNNNNNNGC 1 cut(s) 89
NarI GGCGCC 1 cut(s) 81
NdeII GATC 3 cut(s) 253, 505, 591
NlaIII CATG 1 cut(s) 418
NlaIV GGNNCC 2 cut(s) 82, 255
NmuCI GTSAC 1 cut(s) 533
PciSI GCTCTTC 1 cut(s) 323
PfeI GAWTC 3 cut(s) 239, 411, 564
PkrI GCNGC 1 cut(s) 249
PluTI GGCGCC 1 cut(s) 84
Ppu21I YACGTR 1 cut(s) 277
PpuMI RGGWCCY 1 cut(s) 616
Psp5II RGGWCCY 1 cut(s) 616
PspN4I GGNNCC 2 cut(s) 82, 255
PspPI GGNCC 2 cut(s) 8, 616
PspPPI RGGWCCY 1 cut(s) 616
PstI CTGCAG 1 cut(s) 165
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
SalI GTCGAC 1 cut(s) 541
SapI GCTCTTC 1 cut(s) 323
SaqAI TTAA 1 cut(s) 266
SatI GCNGC 1 cut(s) 248
Sau3AI GATC 3 cut(s) 253, 505, 591
Sau96I GGNCC 2 cut(s) 8, 616
SetI ASST 6 cut(s) 22, 76, 279, 283, 477, 618
SfaNI GCATC 2 cut(s) 181, 451
SfcI CTRYAG 1 cut(s) 161
SfoI GGCGCC 1 cut(s) 82
SinI GGWCC 2 cut(s) 8, 616
SmlI CTYRAG 1 cut(s) 265
SmoI CTYRAG 1 cut(s) 265
Sse9I AATT 1 cut(s) 145
SsiI CCGC 4 cut(s) 113, 349, 418, 693
SspDI GGCGCC 1 cut(s) 80
SspMI CTAG 1 cut(s) 260
TaaI ACNGT 1 cut(s) 547
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 430, 542
TasI AATT 1 cut(s) 145
TfiI GAWTC 3 cut(s) 239, 411, 564
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TseFI GTSAC 1 cut(s) 533
TseI GCWGC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 533
TspGWI ACGGA 1 cut(s) 572
Vha464I CTTAAG 1 cut(s) 265
VpaK11BI GGWCC 2 cut(s) 8, 616
XapI RAATTY 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 542
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.