pycom07g20000

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001330
Physical Location & Seq
Forward (+)
22215427 .. 22216900
1474 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20000.4

Sequence Viewer

Length: 531 bp
ATGGGGCCTCATCCATCCGGGCTCGGCCCTCATGTCCCGCTTCCTGAATATATCGAGAACATGAGAAAGATTGCAAGTCATCTGAAGAGCCTTTCAGATTCAACGCGGATAATCTTTCTTAGTTGTCCTCCAGTGAACGAGGCCGTAATAAATGGAAACATAAGGAGCGGGACTTTCAGCGAGATGATAAGAACCAACGAATTATGCCAACAGTATTCAGAAGCTTGTATAAAGCTTTGCCAGGAAATCAATGTCACATCTGTTGATCTTTTCACTGCAGTTCAGAGAAAAGACGACTGGATGAATACTTGTTTTATAGATGGGATTCATTTTTCTGCTGAAGGGAGCAAAATAGTGGTGGAAGAGATACTCAAGGTACTGAGAGAAGCTGACTGGAAGCCAAGTTTACACTGGAAATCCATGCCAATAGAATTTTCAGAGGATTCACCATATGATATTGCTGCTGCTGACGGAAAGACAACGTTCAATCCCTCCAGCTCGACTTTTTATTGGGAGATTCAGTGGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

177

Amino Acids

19.92

Weight (kDa)

4.99

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 168
AccII CGCG 1 cut(s) 106
AciI CCGC 3 cut(s) 38, 106, 168
AclI AACGTT 1 cut(s) 482
AcsI RAATTY 1 cut(s) 431
AcuI CTGAAG 2 cut(s) 104, 360
AfaI GTAC 1 cut(s) 378
AgsI TTSAA 2 cut(s) 102, 487
AjnI CCWGG 1 cut(s) 240
AjuI GAANNNNNNNTTGG 2 cut(s) 418, 450
AluBI AGCT 4 cut(s) 224, 235, 389, 498
AluI AGCT 4 cut(s) 224, 235, 389, 498
AoxI GGCC 3 cut(s) 5, 25, 141
ApeKI GCWGC 2 cut(s) 461, 464
ApoI RAATTY 1 cut(s) 431
AspS9I GGNCC 2 cut(s) 5, 26
AsuC2I CCSGG 1 cut(s) 19
AsuHPI GGTGA 1 cut(s) 438
BanII GRGCYC 1 cut(s) 24
BbvI GCAGC 2 cut(s) 448, 451
BccI CCATC 2 cut(s) 22, 314
BceAI ACGGC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 242
BcnI CCSGG 1 cut(s) 19
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 2 cut(s) 462, 465
BlsI GCNGC 2 cut(s) 463, 466
Bme1390I CCNGG 2 cut(s) 19, 242
BmgT120I GGNCC 2 cut(s) 5, 26
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 19, 242
BpmI CTGGAG 2 cut(s) 114, 478
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 19
BsaXI ACNNNNNCTCC 1 cut(s) 506
Bse1I ACTGG 4 cut(s) 131, 302, 398, 416
BseBI CCWGG 1 cut(s) 242
BseGI GGATG 3 cut(s) 10, 14, 306
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 4 cut(s) 131, 302, 398, 416
BseXI GCAGC 2 cut(s) 448, 451
Bsh1236I CGCG 1 cut(s) 106
BshFI GGCC 3 cut(s) 7, 27, 143
BsiSI CCGG 1 cut(s) 18
BslFI GGGAC 2 cut(s) 20, 184
BsmFI GGGAC 2 cut(s) 20, 184
BsnI GGCC 3 cut(s) 7, 27, 143
Bsp1286I GDGCHC 1 cut(s) 24
Bsp143I GATC 1 cut(s) 265
BspACI CCGC 3 cut(s) 38, 106, 168
BspANI GGCC 3 cut(s) 7, 27, 143
BspCNI CTCAG 1 cut(s) 372
BspFNI CGCG 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 280
BspQI GCTCTTC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 168
BsrI ACTGG 4 cut(s) 131, 302, 398, 416
BssMI GATC 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 1 cut(s) 213
Bst6I CTCTTC 2 cut(s) 80, 357
BstDEI CTNAG 2 cut(s) 119, 380
BstF5I GGATG 3 cut(s) 10, 14, 306
BstFNI CGCG 1 cut(s) 106
BstKTI GATC 1 cut(s) 268
BstMBI GATC 1 cut(s) 265
BstNI CCWGG 1 cut(s) 242
BstSCI CCNGG 2 cut(s) 17, 240
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 1 cut(s) 106
BstV1I GCAGC 2 cut(s) 448, 451
BsuRI GGCC 3 cut(s) 7, 27, 143
BtsCI GGATG 3 cut(s) 10, 14, 306
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 4 cut(s) 138, 273, 409, 527
Cfr13I GGNCC 2 cut(s) 5, 26
Csp6I GTAC 1 cut(s) 377
CviAII CATG 3 cut(s) 32, 61, 421
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 2 cut(s) 119, 380
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
Eam1104I CTCTTC 2 cut(s) 80, 357
EarI CTCTTC 2 cut(s) 80, 357
Eco24I GRGCYC 1 cut(s) 24
Eco57I CTGAAG 2 cut(s) 104, 360
EcoO109I RGGNCCY 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 24
FaeI CATG 3 cut(s) 35, 64, 424
FaqI GGGAC 2 cut(s) 20, 184
FatI CATG 3 cut(s) 31, 60, 420
FauI CCCGC 2 cut(s) 45, 161
FauNDI CATATG 1 cut(s) 451
Fnu4HI GCNGC 2 cut(s) 462, 465
FokI GGATG 1 cut(s) 313
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 2 cut(s) 462, 465
GluI GCNGC 2 cut(s) 462, 465
GsuI CTGGAG 2 cut(s) 114, 478
HaeIII GGCC 3 cut(s) 7, 27, 143
HapII CCGG 1 cut(s) 18
Hin1II CATG 3 cut(s) 35, 64, 424
HindIII AAGCTT 2 cut(s) 222, 233
HinfI GANTC 4 cut(s) 98, 325, 443, 517
HpaII CCGG 1 cut(s) 18
HphI GGTGA 1 cut(s) 438
Hpy166II GTNNAC 2 cut(s) 136, 407
Hpy188I TCNGA 5 cut(s) 84, 97, 220, 285, 439
Hpy188III TCNNGA 2 cut(s) 44, 55
Hpy8I GTNNAC 2 cut(s) 136, 407
HpyAV CCTTC 1 cut(s) 335
HpyCH4III ACNGT 1 cut(s) 213
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 2 cut(s) 74, 278
HpyF3I CTNAG 2 cut(s) 119, 380
HpySE526I ACGT 1 cut(s) 482
Hsp92II CATG 3 cut(s) 35, 64, 424
Kzo9I GATC 1 cut(s) 265
LguI GCTCTTC 1 cut(s) 80
LmnI GCTCC 2 cut(s) 165, 345
LpnPI CCDG 9 cut(s) 31, 57, 144, 227, 254, 283, 379, 397, 508
Lsp1109I GCAGC 2 cut(s) 448, 451
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 1 cut(s) 253
MalI GATC 1 cut(s) 267
MbiI CCGCTC 1 cut(s) 168
MboI GATC 1 cut(s) 265
MboII GAAGA 2 cut(s) 97, 374
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 2 cut(s) 200, 431
MnlI CCTC 6 cut(s) 18, 39, 133, 138, 433, 502
MspI CCGG 1 cut(s) 18
MspR9I CCNGG 2 cut(s) 19, 242
MvaI CCWGG 1 cut(s) 242
MvnI CGCG 1 cut(s) 106
NciI CCSGG 1 cut(s) 19
NdeI CATATG 1 cut(s) 451
NdeII GATC 1 cut(s) 265
NlaIII CATG 3 cut(s) 35, 64, 424
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 253
PciSI GCTCTTC 1 cut(s) 80
PfeI GAWTC 4 cut(s) 98, 325, 443, 517
PkrI GCNGC 2 cut(s) 463, 466
Psp1406I AACGTT 1 cut(s) 482
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 5, 26
PstI CTGCAG 1 cut(s) 280
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SapI GCTCTTC 1 cut(s) 80
SatI GCNGC 2 cut(s) 462, 465
Sau3AI GATC 1 cut(s) 265
Sau96I GGNCC 2 cut(s) 5, 26
ScrFI CCNGG 2 cut(s) 19, 242
SduI GDGCHC 1 cut(s) 24
SetI ASST 6 cut(s) 226, 237, 378, 391, 485, 500
SfcI CTRYAG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 2 cut(s) 200, 431
SsiI CCGC 3 cut(s) 38, 106, 168
StyD4I CCNGG 2 cut(s) 17, 240
TaaI ACNGT 1 cut(s) 213
TaiI ACGT 1 cut(s) 485
TaqI TCGA 2 cut(s) 54, 500
TasI AATT 2 cut(s) 200, 431
TfiI GAWTC 4 cut(s) 98, 325, 443, 517
TscAI CASTG 4 cut(s) 138, 280, 416, 527
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 2 cut(s) 461, 464
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 2 cut(s) 317, 317
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 4 cut(s) 138, 280, 416, 527
XapI RAATTY 1 cut(s) 431
XcmI CCANNNNNNNNNTGG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.