Rroxscaffold_6G00416930

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001330
Physical Location & Seq
Forward (+)
38779096 .. 38781497
2402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00416930.1

Sequence Viewer

Length: 771 bp
ATGGTCGGACCAATAAGACCTCAGTTTGTGCTGTTTGGTTCGTCCATCGTCCAGTTCAGTTTCAGCAATGGAGGTTGGGGCGCCATCCTTGCTGACTTATATTCTCGTAAGTCAATATGTCTGTTGGCTCAAACTCTCTGTCCCTCCCTCTTTGTTAGCCTAAAGGATGAGGCGGATATACTAGTGCGTGGCTACGCTGGTTGGAATTCAAGGAATGGTCTGCAGGCTTTAGAGCAAGTGTTTCCAAAGGATGCAAGTATCCAGCCATCGTTGGTGATAGTCTATTTTGGTGGTAATGATTCAATGCTGCCGGATCCACTAGGCCTAAGCCTTCACGTACCTCTTCCTGAATATGTAGAAAATATGAGGAAGATTGCTACCCATTTGAAGAGCCTTTCGCAGAAAACCCGCCTTATGTTCCTGACTGCTCCTCCTGTCAACCAGGAACAAATGCGAAAAGCATTTAGTGGAATCATGCGGGAGAATAGTCGAACAAACAAGCAGTTGCAAATGTATTCAGATGCTTGTGTGGAGCTGTGCAGAGAGATGGATATCAAGGCAGTTGATCTCTTTACTTCAATGCAGAAAAGAAATGACTGGTCGACTGTATGCTTTACGGATGGAATCCATTTTTCATCCGAGGGGAGCAAGATCGTGGTGGAGGAGATACTGAAGGTCCTTGAAGAAGCAGACTGGGAACCCAGCCTGCAATGGAACTCATTGCCAACTGAGTTTGAAGAGTTTGCACCTTATACCGCCGCTCGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

256

Amino Acids

28.8

Weight (kDa)

5.33

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 223 2.5e-18 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 217 1.1e-19 GDSL-like Lipase/Acylhydrolase family
DUF459 PF04311 87 - 225 3.5e-07 Protein of unknown function (DUF459)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 80
AccBSI CCGCTC 1 cut(s) 761
AccI GTMKAC 1 cut(s) 602
AciI CCGC 5 cut(s) 173, 409, 478, 756, 759
AclWI GGATC 2 cut(s) 308, 321
AcsI RAATTY 1 cut(s) 205
AcuI CTGAAG 1 cut(s) 692
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 339
AfiI CCNNNNNNNGG 1 cut(s) 762
AgsI TTSAA 6 cut(s) 210, 303, 388, 579, 683, 737
AhlI ACTAGT 1 cut(s) 181
AjnI CCWGG 1 cut(s) 441
AluBI AGCT 1 cut(s) 535
AluI AGCT 1 cut(s) 535
AlwI GGATC 2 cut(s) 308, 321
Ama87I CYCGRG 1 cut(s) 762
AoxI GGCC 1 cut(s) 322
ApeKI GCWGC 1 cut(s) 307
ApoI RAATTY 1 cut(s) 205
ArsI GACNNNNNNTTYG 2 cut(s) 124, 156
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 8, 676
AsuHPI GGTGA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 762
AvaII GGWCC 2 cut(s) 8, 676
BamHI GGATCC 1 cut(s) 313
BanI GGYRCC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 294
BccI CCATC 5 cut(s) 53, 92, 274, 541, 614
BciT130I CCWGG 1 cut(s) 443
BciVI GTATCC 1 cut(s) 269
BcuI ACTAGT 1 cut(s) 181
BfaI CTAG 2 cut(s) 182, 320
BfmI CTRYAG 1 cut(s) 221
BfoI RGCGCY 1 cut(s) 84
BfuI GTATCC 1 cut(s) 269
BisI GCNGC 2 cut(s) 308, 759
BlsI GCNGC 2 cut(s) 309, 760
Bme1390I CCNGG 1 cut(s) 443
Bme18I GGWCC 2 cut(s) 8, 676
BmeT110I CYCGRG 1 cut(s) 762
BmgT120I GGNCC 2 cut(s) 8, 676
BmiI GGNNCC 3 cut(s) 82, 315, 699
BmrFI CCNGG 1 cut(s) 443
BmrI ACTGGG 1 cut(s) 703
BmsI GCATC 2 cut(s) 241, 511
BmuI ACTGGG 1 cut(s) 703
Bpu10I CCTNAGC 1 cut(s) 326
BsaAI YACGTR 1 cut(s) 337
BsaBI GATNNNNATC 1 cut(s) 551
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 639
BsaXI ACNNNNNCTCC 2 cut(s) 415, 445
Bsc4I CCNNNNNNNGG 1 cut(s) 762
Bse1I ACTGG 3 cut(s) 52, 602, 698
Bse3DI GCAATG 3 cut(s) 73, 716, 719
Bse8I GATNNNNATC 1 cut(s) 551
BseBI CCWGG 1 cut(s) 443
BseDI CCNNGG 1 cut(s) 639
BseGI GGATG 5 cut(s) 84, 172, 256, 625, 635
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 1 cut(s) 762
BseMI GCAATG 3 cut(s) 73, 716, 719
BseMII CTCAG 2 cut(s) 35, 720
BseNI ACTGG 3 cut(s) 52, 602, 698
BseRI GAGGAG 2 cut(s) 420, 677
BseXI GCAGC 1 cut(s) 294
BseYI CCCAGC 1 cut(s) 701
BsgI GTGCAG 1 cut(s) 559
BshFI GGCC 1 cut(s) 324
BshNI GGYRCC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 762
BsiSI CCGG 1 cut(s) 311
BslFI GGGAC 1 cut(s) 126
BslI CCNNNNNNNGG 1 cut(s) 762
BsmFI GGGAC 1 cut(s) 126
BsnI GGCC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 762
Bsp143I GATC 3 cut(s) 313, 565, 651
BspACI CCGC 5 cut(s) 173, 409, 478, 756, 759
BspANI GGCC 1 cut(s) 324
BspCNI CTCAG 2 cut(s) 34, 721
BspLI GGNNCC 3 cut(s) 82, 315, 699
BspMAI CTGCAG 1 cut(s) 225
BspPI GGATC 2 cut(s) 308, 321
BspQI GCTCTTC 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 761
BsrDI GCAATG 3 cut(s) 73, 716, 719
BsrI ACTGG 3 cut(s) 52, 602, 698
BssECI CCNNGG 1 cut(s) 639
BssMI GATC 3 cut(s) 313, 565, 651
BssNI GRCGYC 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 443
Bst4CI ACNGT 1 cut(s) 607
Bst6I CTCTTC 3 cut(s) 348, 383, 732
BstACI GRCGYC 1 cut(s) 81
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 2 cut(s) 225, 707
BstDEI CTNAG 3 cut(s) 21, 326, 729
BstF5I GGATG 5 cut(s) 84, 172, 256, 625, 635
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 3 cut(s) 316, 568, 654
BstMBI GATC 3 cut(s) 313, 565, 651
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstNI CCWGG 1 cut(s) 443
BstSCI CCNGG 1 cut(s) 441
BstSFI CTRYAG 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BsuI GTATCC 1 cut(s) 269
BsuRI GGCC 1 cut(s) 324
BtsCI GGATG 5 cut(s) 84, 172, 256, 625, 635
Cac8I GCNNGC 2 cut(s) 225, 707
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 8, 676
Csp6I GTAC 1 cut(s) 338
CviAII CATG 1 cut(s) 475
CviQI GTAC 1 cut(s) 338
DdeI CTNAG 3 cut(s) 21, 326, 729
DinI GGCGCC 1 cut(s) 82
DpnI GATC 3 cut(s) 315, 567, 653
DpnII GATC 3 cut(s) 313, 565, 651
Eam1104I CTCTTC 3 cut(s) 348, 383, 732
EarI CTCTTC 3 cut(s) 348, 383, 732
EciI GGCGGA 1 cut(s) 188
Eco147I AGGCCT 1 cut(s) 324
Eco32I GATATC 1 cut(s) 553
Eco47I GGWCC 2 cut(s) 8, 676
Eco57I CTGAAG 1 cut(s) 692
Eco88I CYCGRG 1 cut(s) 762
EcoO109I RGGNCCY 1 cut(s) 676
EcoRI GAATTC 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 441
EcoRV GATATC 1 cut(s) 553
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
FaeI CATG 1 cut(s) 478
FaiI YATR 9 cut(s) 100, 118, 179, 354, 365, 416, 476, 610, 753
FaqI GGGAC 1 cut(s) 126
FatI CATG 1 cut(s) 474
FauI CCCGC 2 cut(s) 416, 471
FblI GTMKAC 1 cut(s) 602
Fnu4HI GCNGC 2 cut(s) 308, 759
FokI GGATG 5 cut(s) 71, 179, 263, 622, 632
Fsp4HI GCNGC 2 cut(s) 308, 759
FspBI CTAG 2 cut(s) 182, 320
GlaI GCGC 1 cut(s) 82
GluI GCNGC 2 cut(s) 308, 759
GsaI CCCAGC 1 cut(s) 705
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 1 cut(s) 324
HapII CCGG 1 cut(s) 311
HhaI GCGC 1 cut(s) 83
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 1 cut(s) 478
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 2 cut(s) 439, 603
HindII GTYRAC 2 cut(s) 439, 603
HinfI GANTC 3 cut(s) 299, 471, 624
HpaII CCGG 1 cut(s) 311
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 2 cut(s) 439, 603
Hpy188I TCNGA 3 cut(s) 8, 520, 640
Hpy188III TCNNGA 2 cut(s) 347, 421
Hpy8I GTNNAC 2 cut(s) 439, 603
HpyAV CCTTC 2 cut(s) 341, 667
HpyCH4III ACNGT 1 cut(s) 607
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 7 cut(s) 223, 254, 508, 540, 583, 709, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 3 cut(s) 21, 326, 729
HpySE526I ACGT 1 cut(s) 336
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 1 cut(s) 478
HspAI GCGC 1 cut(s) 81
KasI GGCGCC 1 cut(s) 80
Kzo9I GATC 3 cut(s) 313, 565, 651
LguI GCTCTTC 1 cut(s) 383
LmnI GCTCC 3 cut(s) 433, 532, 645
Lsp1109I GCAGC 1 cut(s) 294
LweI GCATC 2 cut(s) 241, 511
MaeI CTAG 2 cut(s) 182, 320
MaeII ACGT 1 cut(s) 336
MalI GATC 3 cut(s) 315, 567, 653
MbiI CCGCTC 1 cut(s) 761
MboI GATC 3 cut(s) 313, 565, 651
MboII GAAGA 5 cut(s) 335, 382, 400, 695, 749
MflI RGATCY 1 cut(s) 313
MluCI AATT 1 cut(s) 205
Mly113I GGCGCC 1 cut(s) 81
MmeI TCCRAC 1 cut(s) 182
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 443
MvaI CCWGG 1 cut(s) 443
MwoI GCNNNNNNNGC 1 cut(s) 89
NarI GGCGCC 1 cut(s) 81
NdeII GATC 3 cut(s) 313, 565, 651
NlaIII CATG 1 cut(s) 478
NlaIV GGNNCC 3 cut(s) 82, 315, 699
PceI AGGCCT 1 cut(s) 324
PciSI GCTCTTC 1 cut(s) 383
PfeI GAWTC 3 cut(s) 299, 471, 624
PkrI GCNGC 2 cut(s) 309, 760
PluTI GGCGCC 1 cut(s) 84
Ppu21I YACGTR 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 676
Psp5II RGGWCCY 1 cut(s) 676
Psp6I CCWGG 1 cut(s) 441
PspFI CCCAGC 1 cut(s) 701
PspGI CCWGG 1 cut(s) 441
PspN4I GGNNCC 3 cut(s) 82, 315, 699
PspPI GGNCC 2 cut(s) 8, 676
PspPPI RGGWCCY 1 cut(s) 676
PstI CTGCAG 1 cut(s) 225
PsuI RGATCY 1 cut(s) 313
RsaI GTAC 1 cut(s) 339
RsaNI GTAC 1 cut(s) 338
SalI GTCGAC 1 cut(s) 601
SapI GCTCTTC 1 cut(s) 383
SatI GCNGC 2 cut(s) 308, 759
Sau3AI GATC 3 cut(s) 313, 565, 651
Sau96I GGNCC 2 cut(s) 8, 676
ScrFI CCNGG 1 cut(s) 443
SetI ASST 7 cut(s) 22, 76, 339, 343, 537, 678, 751
SfaNI GCATC 2 cut(s) 241, 511
SfcI CTRYAG 1 cut(s) 221
SfoI GGCGCC 1 cut(s) 82
SinI GGWCC 2 cut(s) 8, 676
SpeI ACTAGT 1 cut(s) 181
Sse9I AATT 1 cut(s) 205
SseBI AGGCCT 1 cut(s) 324
SsiI CCGC 5 cut(s) 173, 409, 478, 756, 759
SspDI GGCGCC 1 cut(s) 80
SspMI CTAG 2 cut(s) 182, 320
StuI AGGCCT 1 cut(s) 324
StyD4I CCNGG 1 cut(s) 441
TaaI ACNGT 1 cut(s) 607
TaiI ACGT 1 cut(s) 339
TaqI TCGA 2 cut(s) 490, 602
TasI AATT 1 cut(s) 205
TauI GCSGC 1 cut(s) 761
TfiI GAWTC 3 cut(s) 299, 471, 624
TseI GCWGC 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 624
TspGWI ACGGA 1 cut(s) 632
VpaK11BI GGWCC 2 cut(s) 8, 676
XapI RAATTY 1 cut(s) 205
XmiI GTMKAC 1 cut(s) 602
XspI CTAG 2 cut(s) 182, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.