Rroxscaffold_4G00286810

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001330
Physical Location & Seq
Reverse (-)
7760908 .. 7763465
2558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286810.1

Sequence Viewer

Length: 765 bp
ATGGTGGGACCCCCGAGGCCTCAGTTTGTTCTGTTCGGATCGTCCATAGTCCAGCTCAGCTTCAGCCACGGCGGCTGGGGCTCCGTTCTCTCCGACCTCTACGCTCGCAAAGCAGACATATTGCTGCGTGGTTACTTCGGATGGAATTCACGGCGTGCACTGGAGGTGCTTGATCAAGTTTTTCCCAAGGATGCAGCTGTTCAACCTTCTTTGGTGATAGTCTATTTTGGCGGGAATGATTCTATGGGGCCTCATCCATCTGGGCTAGGACCTCATGTACCACTTCCTGAGTATATTGAGAACATGAGGAAGATTGCAAATCATCTCCAGAGCCTTTCAGATTCAACACGCATCATTTTTCTTAGTTGTCCTCCCGTGAATGAGGCCACAGTTCGTGCAAATAAAAGTCCTTATCTCAGCGAGTTAGTAAGAACGAATGAGCTATGCCAACAATATTCCGAAGCTTGTATAAAGCTATGCCAGGAACTGGATATCAAGGTGGTTGATCTTTTTACGGCAATACAGAAAGGAGAAAACTGTTTTACAGATGGGATTCATTTATCAGCTGAAGGGAGCAAAATAGTGGCGGAGGAGATACTGAAAGTACTGAGAGAAGCTGATTGGAAGCCAAGTTTACACTGGAAATCCATGCCAACAGAATTTGCAGAGGATTCACCACATGATCTTGTTGCTGCTGATGGAAAGACGACGTTAAATCCCTCCGAATGGACGTTTTATAGAGATTTTCACTGGAACTCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

254

Amino Acids

28.42

Weight (kDa)

5.61

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 199 9.4e-23 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 193 1.8e-21 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 487
AciI CCGC 3 cut(s) 72, 231, 587
AclWI GGATC 1 cut(s) 46
AcsI RAATTY 2 cut(s) 145, 659
AcuI CTGAAG 2 cut(s) 46, 588
AdeI CACNNNGTG 1 cut(s) 155
AfaI GTAC 2 cut(s) 279, 606
AfiI CCNNNNNNNGG 2 cut(s) 487, 726
AgsI TTSAA 2 cut(s) 203, 345
AjnI CCWGG 1 cut(s) 480
AluBI AGCT 8 cut(s) 55, 60, 197, 442, 464, 475, 566, 617
AluI AGCT 8 cut(s) 55, 60, 197, 442, 464, 475, 566, 617
Alw21I GWGCWC 1 cut(s) 160
Alw44I GTGCAC 1 cut(s) 156
AlwI GGATC 1 cut(s) 46
AlwNI CAGNNNCTG 1 cut(s) 487
Ama87I CYCGRG 1 cut(s) 13
AoxI GGCC 3 cut(s) 17, 248, 384
ApaLI GTGCAC 1 cut(s) 156
ApeKI GCWGC 3 cut(s) 124, 194, 692
ApoI RAATTY 2 cut(s) 145, 659
AspS9I GGNCC 3 cut(s) 8, 248, 269
AsuHPI GGTGA 2 cut(s) 226, 666
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 2 cut(s) 8, 269
BaeGI GKGCMC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 3 cut(s) 111, 206, 679
BccI CCATC 4 cut(s) 135, 265, 542, 692
BceAI ACGGC 3 cut(s) 85, 167, 531
BciT130I CCWGG 1 cut(s) 482
BclI TGATCA 1 cut(s) 172
BfaI CTAG 1 cut(s) 266
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 4 cut(s) 73, 125, 195, 693
BlpI GCTNAGC 1 cut(s) 56
BlsI GCNGC 4 cut(s) 74, 126, 196, 694
BmcAI AGTACT 1 cut(s) 606
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 2 cut(s) 8, 269
BmeT110I CYCGRG 1 cut(s) 13
BmgT120I GGNCC 3 cut(s) 8, 248, 269
BmiI GGNNCC 4 cut(s) 9, 10, 82, 249
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 2 cut(s) 181, 360
BpmI CTGGAG 2 cut(s) 182, 311
Bpu1102I GCTNAGC 1 cut(s) 56
BsaJI CCNNGG 3 cut(s) 14, 67, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 487, 726
Bse1I ACTGG 4 cut(s) 165, 492, 644, 755
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 3 cut(s) 14, 67, 186
BseGI GGATG 3 cut(s) 146, 196, 253
BseLI CCNNNNNNNGG 2 cut(s) 487, 726
BseMII CTCAG 5 cut(s) 35, 70, 279, 430, 599
BseNI ACTGG 4 cut(s) 165, 492, 644, 755
BseRI GAGGAG 1 cut(s) 605
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 3 cut(s) 111, 206, 679
BseYI CCCAGC 1 cut(s) 75
BshFI GGCC 3 cut(s) 19, 250, 386
BsiHKAI GWGCWC 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 13
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 2 cut(s) 487, 726
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 3 cut(s) 19, 250, 386
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 2 cut(s) 83, 160
Bsp143I GATC 4 cut(s) 38, 172, 505, 682
Bsp1720I GCTNAGC 1 cut(s) 56
BspACI CCGC 3 cut(s) 72, 231, 587
BspANI GGCC 3 cut(s) 19, 250, 386
BspCNI CTCAG 5 cut(s) 34, 69, 280, 429, 600
BspLI GGNNCC 4 cut(s) 9, 10, 82, 249
BspPI GGATC 1 cut(s) 46
BsrI ACTGG 4 cut(s) 165, 492, 644, 755
BssECI CCNNGG 3 cut(s) 14, 67, 186
BssMI GATC 4 cut(s) 38, 172, 505, 682
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 2 cut(s) 391, 539
BstC8I GCNNGC 2 cut(s) 106, 156
BstDEI CTNAG 6 cut(s) 21, 56, 288, 362, 416, 608
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 3 cut(s) 146, 196, 253
BstKTI GATC 4 cut(s) 41, 175, 508, 685
BstMBI GATC 4 cut(s) 38, 172, 505, 682
BstMWI GCNNNNNNNGC 3 cut(s) 72, 78, 110
BstNI CCWGG 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 480
BstSLI GKGCMC 1 cut(s) 160
BstV1I GCAGC 3 cut(s) 111, 206, 679
BsuRI GGCC 3 cut(s) 19, 250, 386
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 3 cut(s) 146, 196, 253
BtsIMutI CAGTG 3 cut(s) 158, 637, 748
Cac8I GCNNGC 2 cut(s) 106, 156
CaiI CAGNNNCTG 1 cut(s) 487
Cfr13I GGNCC 3 cut(s) 8, 248, 269
Csp6I GTAC 2 cut(s) 278, 605
CviAII CATG 4 cut(s) 275, 304, 649, 680
CviQI GTAC 2 cut(s) 278, 605
DdeI CTNAG 6 cut(s) 21, 56, 288, 362, 416, 608
DpnI GATC 4 cut(s) 40, 174, 507, 684
DpnII GATC 4 cut(s) 38, 172, 505, 682
DraIII CACNNNGTG 1 cut(s) 155
EciI GGCGGA 1 cut(s) 602
Eco130I CCWWGG 1 cut(s) 186
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 83
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 2 cut(s) 8, 269
Eco57I CTGAAG 2 cut(s) 46, 588
Eco88I CYCGRG 1 cut(s) 13
EcoO109I RGGNCCY 3 cut(s) 8, 248, 269
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 480
EcoRV GATATC 1 cut(s) 493
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 4 cut(s) 278, 307, 652, 683
FaqI GGGAC 1 cut(s) 21
FatI CATG 4 cut(s) 274, 303, 648, 679
FauI CCCGC 1 cut(s) 224
FbaI TGATCA 1 cut(s) 172
Fnu4HI GCNGC 4 cut(s) 73, 125, 195, 693
FokI GGATG 3 cut(s) 153, 203, 240
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 4 cut(s) 73, 125, 195, 693
FspBI CTAG 1 cut(s) 266
GluI GCNGC 4 cut(s) 73, 125, 195, 693
GsaI CCCAGC 1 cut(s) 79
GsuI CTGGAG 2 cut(s) 182, 311
HaeIII GGCC 3 cut(s) 19, 250, 386
Hin1II CATG 4 cut(s) 278, 307, 652, 683
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 4 cut(s) 239, 341, 553, 671
HphI GGTGA 2 cut(s) 226, 666
Hpy166II GTNNAC 2 cut(s) 158, 635
Hpy188I TCNGA 6 cut(s) 38, 94, 140, 340, 460, 724
Hpy188III TCNNGA 2 cut(s) 287, 328
Hpy8I GTNNAC 2 cut(s) 158, 635
Hpy99I CGWCG 1 cut(s) 712
HpyAV CCTTC 2 cut(s) 216, 563
HpyCH4III ACNGT 2 cut(s) 391, 539
HpyCH4IV ACGT 2 cut(s) 710, 731
HpyCH4V TGCA 5 cut(s) 158, 194, 317, 398, 665
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 78, 110
HpyF3I CTNAG 6 cut(s) 21, 56, 288, 362, 416, 608
HpySE526I ACGT 2 cut(s) 710, 731
Hsp92II CATG 4 cut(s) 278, 307, 652, 683
KflI GGGWCCC 1 cut(s) 8
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 4 cut(s) 38, 172, 505, 682
LmnI GCTCC 2 cut(s) 86, 573
Lsp1109I GCAGC 3 cut(s) 111, 206, 679
LweI GCATC 2 cut(s) 181, 360
MaeI CTAG 1 cut(s) 266
MaeII ACGT 2 cut(s) 710, 731
MaeIII GTNAC 1 cut(s) 131
MalI GATC 4 cut(s) 40, 174, 507, 684
MboI GATC 4 cut(s) 38, 172, 505, 682
MboII GAAGA 1 cut(s) 322
MhlI GDGCHC 2 cut(s) 83, 160
MluCI AATT 2 cut(s) 145, 659
MmeI TCCRAC 1 cut(s) 117
MseI TTAA 1 cut(s) 713
MspA1I CMGCKG 2 cut(s) 197, 566
MspR9I CCNGG 1 cut(s) 482
MvaI CCWGG 1 cut(s) 482
MwoI GCNNNNNNNGC 3 cut(s) 72, 78, 110
NdeII GATC 4 cut(s) 38, 172, 505, 682
NlaIII CATG 4 cut(s) 278, 307, 652, 683
NlaIV GGNNCC 4 cut(s) 9, 10, 82, 249
PceI AGGCCT 1 cut(s) 19
PfeI GAWTC 4 cut(s) 239, 341, 553, 671
PflMI CCANNNNNTGG 1 cut(s) 487
PkrI GCNGC 4 cut(s) 74, 126, 196, 694
PpuMI RGGWCCY 2 cut(s) 8, 269
Psp5II RGGWCCY 2 cut(s) 8, 269
Psp6I CCWGG 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 4 cut(s) 9, 10, 82, 249
PspPI GGNCC 3 cut(s) 8, 248, 269
PspPPI RGGWCCY 2 cut(s) 8, 269
PstNI CAGNNNCTG 1 cut(s) 487
PvuII CAGCTG 2 cut(s) 197, 566
RsaI GTAC 2 cut(s) 279, 606
RsaNI GTAC 2 cut(s) 278, 605
SaqAI TTAA 1 cut(s) 713
SatI GCNGC 4 cut(s) 73, 125, 195, 693
Sau3AI GATC 4 cut(s) 38, 172, 505, 682
Sau96I GGNCC 3 cut(s) 8, 248, 269
ScaI AGTACT 1 cut(s) 606
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 2 cut(s) 83, 160
SfaNI GCATC 2 cut(s) 181, 360
SinI GGWCC 2 cut(s) 8, 269
Sse9I AATT 2 cut(s) 145, 659
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 3 cut(s) 72, 231, 587
SspI AATATT 1 cut(s) 455
SspMI CTAG 1 cut(s) 266
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 480
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 2 cut(s) 391, 539
TaiI ACGT 2 cut(s) 713, 734
TasI AATT 2 cut(s) 145, 659
TatI WGTACW 1 cut(s) 604
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 4 cut(s) 239, 341, 553, 671
Tru1I TTAA 1 cut(s) 713
Tru9I TTAA 1 cut(s) 713
TscAI CASTG 3 cut(s) 165, 644, 755
TseI GCWGC 3 cut(s) 124, 194, 692
TspDTI ATGAA 1 cut(s) 545
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 3 cut(s) 165, 644, 755
Van91I CCANNNNNTGG 1 cut(s) 487
VneI GTGCAC 1 cut(s) 156
VpaK11BI GGWCC 2 cut(s) 8, 269
XapI RAATTY 2 cut(s) 145, 659
XcmI CCANNNNNNNNNTGG 1 cut(s) 636
XspI CTAG 1 cut(s) 266
ZrmI AGTACT 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.