FvH4_7g24410

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001330
Physical Location & Seq
Forward (+)
18850393 .. 18853499
3107 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24410.t2

Sequence Viewer

Length: 678 bp
ATGGTAGGACCGGCAAGGCCTCAGTTTGTTCTGTTCGGATCTTCCATAGTCCAGCTCAGCTTCAGCCATGGCGGCTGGGGCGCCATTCTCTCCGACCTCTACGCCCGCAAAGCAGACATATTGCTGCGTGGTTACTTTGGATGGAATTCACGGCGTGCTCTGGAGGTGCTTGAACAAGTTTTTCCCAAGGATGCAGCTGTGCAACCTTCTTTGGTGATAGTCTATTTTGGCGGTAATGATTCTATGGGGGCTCATCCATCTGGGCTAGGACCTCATGTACCACTTCCTGAGTATATTGAGAACATGAGGAAGATTGCAAGTCATATCCAGAGCCTTTCAGATTCAACGCGTATCATTGTTCTTAGTTGTCCTCCTGTTAATGAGGCCACGATTCGTGCAAATAAAAGTCCCTATCTCAGCGAGTTAGTAAGAACGAATGAGCTATGCCAACAATATTCTGAAGCCTGTATAAGGCTATGCCAGGAAATGGATATCAAGGTGGTTGATCTATTTACAGCAATACAGAAAGGAGATAACTGTTTTACCGCACCCGTCCATCTCCGCCGTCCATTTGACGCGGACTTGGCAGAGCCTTTTGACTTTTTCAAAATGGATGGCGGAGATGACGGGTGCGCTGAATACGCTCTCCTCTTTGACATGCACTCTTCTCATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

226

Amino Acids

24.96

Weight (kDa)

5.32

Isoelectric Point (pI)

54.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 199 2.1e-23 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 193 6.9e-22 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 80
AccB7I CCANNNNNTGG 1 cut(s) 487
AccII CGCG 2 cut(s) 349, 578
AciI CCGC 7 cut(s) 72, 106, 231, 546, 562, 578, 618
AclWI GGATC 1 cut(s) 46
AcsI RAATTY 1 cut(s) 145
AcuI CTGAAG 2 cut(s) 46, 480
AcyI GRCGYC 1 cut(s) 81
AdeI CACNNNGTG 1 cut(s) 155
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 2 cut(s) 471, 487
AflIII ACRYGT 1 cut(s) 347
AgsI TTSAA 3 cut(s) 173, 345, 607
AjnI CCWGG 1 cut(s) 480
AluBI AGCT 4 cut(s) 55, 60, 197, 442
AluI AGCT 4 cut(s) 55, 60, 197, 442
Alw21I GWGCWC 1 cut(s) 160
AlwI GGATC 1 cut(s) 46
AoxI GGCC 2 cut(s) 17, 384
ApeKI GCWGC 2 cut(s) 124, 194
ApoI RAATTY 1 cut(s) 145
AspLEI GCGC 2 cut(s) 83, 635
AspS9I GGNCC 2 cut(s) 8, 269
AsuHPI GGTGA 1 cut(s) 226
AvaII GGWCC 2 cut(s) 8, 269
BanI GGYRCC 1 cut(s) 80
BanII GRGCYC 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 2 cut(s) 111, 206
BccI CCATC 4 cut(s) 135, 265, 564, 608
BceAI ACGGC 2 cut(s) 167, 549
BciT130I CCWGG 1 cut(s) 482
BfaI CTAG 1 cut(s) 266
BfoI RGCGCY 1 cut(s) 84
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 3 cut(s) 73, 125, 195
BlpI GCTNAGC 1 cut(s) 56
BlsI GCNGC 3 cut(s) 74, 126, 196
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 2 cut(s) 8, 269
BmgT120I GGNCC 2 cut(s) 8, 269
BmiI GGNNCC 1 cut(s) 82
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 1 cut(s) 181
BpmI CTGGAG 1 cut(s) 182
Bpu1102I GCTNAGC 1 cut(s) 56
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 67, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 471, 487
Bse118I RCCGGY 1 cut(s) 10
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 2 cut(s) 67, 186
BseGI GGATG 4 cut(s) 146, 196, 253, 619
BseLI CCNNNNNNNGG 2 cut(s) 471, 487
BseMII CTCAG 4 cut(s) 35, 70, 279, 430
BseRI GAGGAG 1 cut(s) 638
BseXI GCAGC 2 cut(s) 111, 206
BseYI CCCAGC 1 cut(s) 75
Bsh1236I CGCG 2 cut(s) 349, 578
BshFI GGCC 2 cut(s) 19, 386
BshNI GGYRCC 1 cut(s) 80
BsiHKAI GWGCWC 1 cut(s) 160
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 393
BslI CCNNNNNNNGG 2 cut(s) 471, 487
BsmFI GGGAC 1 cut(s) 393
BsnI GGCC 2 cut(s) 19, 386
Bsp1286I GDGCHC 2 cut(s) 160, 253
Bsp143I GATC 2 cut(s) 38, 505
Bsp1720I GCTNAGC 1 cut(s) 56
Bsp19I CCATGG 1 cut(s) 67
BspACI CCGC 7 cut(s) 72, 106, 231, 546, 562, 578, 618
BspANI GGCC 2 cut(s) 19, 386
BspCNI CTCAG 4 cut(s) 34, 69, 280, 429
BspFNI CGCG 2 cut(s) 349, 578
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 1 cut(s) 82
BspPI GGATC 1 cut(s) 46
BspT107I GGYRCC 1 cut(s) 80
BsrFI RCCGGY 1 cut(s) 10
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 67, 186
BssMI GATC 2 cut(s) 38, 505
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 2 cut(s) 67, 186
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 1 cut(s) 539
Bst6I CTCTTC 1 cut(s) 670
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 106, 156
BstDEI CTNAG 5 cut(s) 21, 56, 288, 362, 416
BstDSI CCRYGG 1 cut(s) 67
BstENI CCTNNNNNAGG 1 cut(s) 469
BstF5I GGATG 4 cut(s) 146, 196, 253, 619
BstFNI CGCG 2 cut(s) 349, 578
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 2 cut(s) 83, 635
BstKTI GATC 2 cut(s) 41, 508
BstMBI GATC 2 cut(s) 38, 505
BstMWI GCNNNNNNNGC 5 cut(s) 72, 78, 110, 584, 641
BstNI CCWGG 1 cut(s) 482
BstNSI RCATGY 1 cut(s) 661
BstSCI CCNGG 1 cut(s) 480
BstUI CGCG 2 cut(s) 349, 578
BstV1I GCAGC 2 cut(s) 111, 206
BstX2I RGATCY 1 cut(s) 38
BstYI RGATCY 1 cut(s) 38
BsuRI GGCC 2 cut(s) 19, 386
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 4 cut(s) 146, 196, 253, 619
Cac8I GCNNGC 2 cut(s) 106, 156
CciI TCATGA 1 cut(s) 670
CfoI GCGC 2 cut(s) 83, 635
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 2 cut(s) 8, 269
CseI GACGC 1 cut(s) 584
Csp6I GTAC 1 cut(s) 278
CviAII CATG 5 cut(s) 68, 275, 304, 658, 671
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 5 cut(s) 21, 56, 288, 362, 416
DinI GGCGCC 1 cut(s) 82
DpnI GATC 2 cut(s) 40, 507
DpnII GATC 2 cut(s) 38, 505
DraIII CACNNNGTG 1 cut(s) 155
Eam1104I CTCTTC 1 cut(s) 670
EarI CTCTTC 1 cut(s) 670
EciI GGCGGA 2 cut(s) 551, 633
Eco130I CCWWGG 2 cut(s) 67, 186
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 253
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 2 cut(s) 8, 269
Eco57I CTGAAG 2 cut(s) 46, 480
EcoNI CCTNNNNNAGG 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 269
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 480
EcoRV GATATC 1 cut(s) 493
EcoT14I CCWWGG 2 cut(s) 67, 186
EcoT38I GRGCYC 1 cut(s) 253
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
ErhI CCWWGG 2 cut(s) 67, 186
FaeI CATG 5 cut(s) 71, 278, 307, 661, 674
FaqI GGGAC 1 cut(s) 393
FatI CATG 5 cut(s) 67, 274, 303, 657, 670
FauI CCCGC 1 cut(s) 113
Fnu4HI GCNGC 3 cut(s) 73, 125, 195
FokI GGATG 4 cut(s) 153, 203, 240, 626
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 3 cut(s) 73, 125, 195
FspBI CTAG 1 cut(s) 266
GlaI GCGC 2 cut(s) 82, 634
GluI GCNGC 3 cut(s) 73, 125, 195
GsaI CCCAGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 182
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 2 cut(s) 19, 386
HapII CCGG 1 cut(s) 11
HgaI GACGC 1 cut(s) 584
HhaI GCGC 2 cut(s) 83, 635
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 5 cut(s) 71, 278, 307, 661, 674
Hin6I GCGC 2 cut(s) 81, 633
HinP1I GCGC 2 cut(s) 81, 633
HinfI GANTC 3 cut(s) 239, 341, 391
HpaII CCGG 1 cut(s) 11
HphI GGTGA 1 cut(s) 226
Hpy188I TCNGA 4 cut(s) 38, 94, 340, 460
Hpy188III TCNNGA 4 cut(s) 161, 287, 328, 671
HpyAV CCTTC 1 cut(s) 216
HpyCH4III ACNGT 1 cut(s) 539
HpyCH4V TGCA 5 cut(s) 194, 202, 317, 398, 661
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 78, 110, 584, 641
HpyF3I CTNAG 5 cut(s) 21, 56, 288, 362, 416
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 5 cut(s) 71, 278, 307, 661, 674
HspAI GCGC 2 cut(s) 81, 633
KasI GGCGCC 1 cut(s) 80
Kzo9I GATC 2 cut(s) 38, 505
Lsp1109I GCAGC 2 cut(s) 111, 206
LweI GCATC 1 cut(s) 181
MaeI CTAG 1 cut(s) 266
MaeIII GTNAC 1 cut(s) 131
MalI GATC 2 cut(s) 40, 507
MboI GATC 2 cut(s) 38, 505
MboII GAAGA 3 cut(s) 33, 322, 657
MflI RGATCY 1 cut(s) 38
MhlI GDGCHC 2 cut(s) 160, 253
MluCI AATT 1 cut(s) 145
MluI ACGCGT 1 cut(s) 347
Mly113I GGCGCC 1 cut(s) 81
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 8 cut(s) 30, 107, 157, 282, 300, 376, 381, 659
MseI TTAA 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 1 cut(s) 482
MvaI CCWGG 1 cut(s) 482
MvnI CGCG 2 cut(s) 349, 578
MwoI GCNNNNNNNGC 5 cut(s) 72, 78, 110, 584, 641
NarI GGCGCC 1 cut(s) 81
NcoI CCATGG 1 cut(s) 67
NdeII GATC 2 cut(s) 38, 505
NlaIII CATG 5 cut(s) 71, 278, 307, 661, 674
NlaIV GGNNCC 1 cut(s) 82
NspI RCATGY 1 cut(s) 661
PagI TCATGA 1 cut(s) 670
PceI AGGCCT 1 cut(s) 19
PfeI GAWTC 3 cut(s) 239, 341, 391
PflMI CCANNNNNTGG 1 cut(s) 487
PkrI GCNGC 3 cut(s) 74, 126, 196
PluTI GGCGCC 1 cut(s) 84
PpuMI RGGWCCY 1 cut(s) 269
Psp5II RGGWCCY 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 1 cut(s) 82
PspPI GGNCC 2 cut(s) 8, 269
PspPPI RGGWCCY 1 cut(s) 269
PsuI RGATCY 1 cut(s) 38
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
SaqAI TTAA 1 cut(s) 378
SatI GCNGC 3 cut(s) 73, 125, 195
Sau3AI GATC 2 cut(s) 38, 505
Sau96I GGNCC 2 cut(s) 8, 269
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 2 cut(s) 160, 253
SetI ASST 9 cut(s) 57, 62, 99, 168, 199, 208, 274, 444, 501
SfaNI GCATC 1 cut(s) 181
SfoI GGCGCC 1 cut(s) 82
SinI GGWCC 2 cut(s) 8, 269
Sse9I AATT 1 cut(s) 145
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 7 cut(s) 72, 106, 231, 546, 562, 578, 618
SspDI GGCGCC 1 cut(s) 80
SspI AATATT 1 cut(s) 455
SspMI CTAG 1 cut(s) 266
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 480
StyI CCWWGG 2 cut(s) 67, 186
TaaI ACNGT 1 cut(s) 539
TasI AATT 1 cut(s) 145
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 3 cut(s) 239, 341, 391
Tru1I TTAA 1 cut(s) 378
Tru9I TTAA 1 cut(s) 378
TseI GCWGC 2 cut(s) 124, 194
Van91I CCANNNNNTGG 1 cut(s) 487
VpaK11BI GGWCC 2 cut(s) 8, 269
XagI CCTNNNNNAGG 1 cut(s) 469
XapI RAATTY 1 cut(s) 145
XceI RCATGY 1 cut(s) 661
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.