RLG00000024807

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001330
Physical Location & Seq
Forward (+)
39249594 .. 39252484
2891 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024807

Sequence Viewer

Length: 783 bp
ATGGTGGGACCTTCAAGACCTCAGTTTGTGCTGTTTGGCTCCTCCATCGTTCAGTACAGTTTCAGCAATGGAGGCTGGGGTGCCATTCTTGCAGACTTATATTCTCGTAAGGCTGATATACTTCTTCGAGGATACGCTGGTTGGAACTCGAGGCGTGCTCTAAAGGTTTTAGAGCAGGTTTTTCCAAAGGATGCAACTATTCAGCCATCATTGGTGATAGTCTATTTCGGTGGTAATGATTCAATGCGCCCTAATCCACTAGGCCTAGGCCCTCATGTACCTCTTTCTGAATATGTAGAAAATATGAGGAAGATTGCTACTCATTTACAGAGCCTTTCAGAGAAAACTCGCATTATCTTTCTCTCTGCTCCTCCTGTCAACGAGGAACAAATTCGTGAGCATTTAAGCGGCATAATTGGGGAGCTGAATCGAACAAACAAGCTATGCAAAATATATTCAGATGCTTGTTTAGAGCTGTGCAGAGAGATGGATATCAAGGCCGTTGATCTCTTCACTTCAATTCAGAAGAGAAAAGACTGGGAAACTACTTGCTTTATTGATGGAGTCCATTTTTCATCTGAGGGGAGCAAGATTGTGGCGGAGGAGATATTGAAGGTCCTTAAAGAGGCGAACTGGGAAAGCAGCCTGCATTGGACGTCATTGGCAACGGAATTCGGAGATGATTCCCCTTATGATCCCGTCGGTATTGATGGAAAAACTATGAATGTCTCAGAGGATGGCGTTGCGAGACTTATGCAGTGGGGAATAAGCAACGGGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

261

Amino Acids

29.05

Weight (kDa)

5.61

Isoelectric Point (pI)

37.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 203 3.6e-22 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 197 6e-21 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 659
Acc36I ACCTGC 1 cut(s) 166
AccB1I GGYRCC 1 cut(s) 80
AciI CCGC 2 cut(s) 408, 599
AclWI GGATC 1 cut(s) 689
AcsI RAATTY 2 cut(s) 390, 671
AcyI GRCGYC 1 cut(s) 656
AfaI GTAC 2 cut(s) 56, 279
AfiI CCNNNNNNNGG 1 cut(s) 625
AgsI TTSAA 4 cut(s) 15, 243, 519, 613
AluBI AGCT 3 cut(s) 424, 442, 475
AluI AGCT 3 cut(s) 424, 442, 475
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 2 cut(s) 733, 742
AlwI GGATC 1 cut(s) 689
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 3 cut(s) 262, 268, 498
ApeKI GCWGC 1 cut(s) 642
ApoI RAATTY 2 cut(s) 390, 671
Asp700I GAANNNNTTC 1 cut(s) 390
AspA2I CCTAGG 1 cut(s) 265
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 3 cut(s) 8, 269, 616
AsuHPI GGTGA 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 2 cut(s) 8, 616
AvrII CCTAGG 1 cut(s) 265
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BanI GGYRCC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 1 cut(s) 654
BccI CCATC 6 cut(s) 53, 214, 481, 554, 704, 731
BceAI ACGGC 1 cut(s) 485
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BciVI GTATCC 1 cut(s) 125
BcoDI GTCTC 2 cut(s) 733, 742
BfaI CTAG 2 cut(s) 260, 266
BfuAI ACCTGC 1 cut(s) 166
BfuI GTATCC 1 cut(s) 125
BisI GCNGC 2 cut(s) 409, 643
BlnI CCTAGG 1 cut(s) 265
BlsI GCNGC 2 cut(s) 410, 644
Bme18I GGWCC 2 cut(s) 8, 616
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 3 cut(s) 8, 269, 616
BmiI GGNNCC 3 cut(s) 9, 40, 82
BmrI ACTGGG 2 cut(s) 547, 643
BmsI GCATC 2 cut(s) 181, 451
BmuI ACTGGG 2 cut(s) 547, 643
BplI GAGNNNNNCTC 2 cut(s) 142, 174
BsaBI GATNNNNATC 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 656
BsaJI CCNNGG 1 cut(s) 265
Bsc4I CCNNNNNNNGG 1 cut(s) 625
Bse1I ACTGG 2 cut(s) 542, 638
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 491
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 2 cut(s) 196, 742
BseJI GATNNNNATC 1 cut(s) 491
BseLI CCNNNNNNNGG 1 cut(s) 625
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 3 cut(s) 35, 570, 744
BseNI ACTGG 2 cut(s) 542, 638
BseRI GAGGAG 3 cut(s) 31, 360, 617
BseXI GCAGC 1 cut(s) 654
BseYI CCCAGC 1 cut(s) 75
BsgI GTGCAG 1 cut(s) 499
BshFI GGCC 3 cut(s) 264, 270, 500
BshNI GGYRCC 1 cut(s) 80
BsiHKAI GWGCWC 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 148
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 1 cut(s) 625
BsmAI GTCTC 2 cut(s) 733, 742
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 3 cut(s) 264, 270, 500
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 160
Bsp143I GATC 2 cut(s) 505, 694
BspACI CCGC 2 cut(s) 408, 599
BspANI GGCC 3 cut(s) 264, 270, 500
BspCNI CTCAG 3 cut(s) 34, 571, 743
BspLI GGNNCC 3 cut(s) 9, 40, 82
BspMI ACCTGC 1 cut(s) 166
BspPI GGATC 1 cut(s) 689
BspT107I GGYRCC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 73
BsrI ACTGG 2 cut(s) 542, 638
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 2 cut(s) 505, 694
BssNI GRCGYC 1 cut(s) 656
BssT1I CCWWGG 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 2 cut(s) 515, 521
BstACI GRCGYC 1 cut(s) 656
BstC8I GCNNGC 2 cut(s) 156, 647
BstDEI CTNAG 3 cut(s) 21, 579, 730
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 2 cut(s) 196, 742
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 2 cut(s) 508, 697
BstMAI GTCTC 2 cut(s) 733, 742
BstMBI GATC 2 cut(s) 505, 694
BstMWI GCNNNNNNNGC 2 cut(s) 72, 89
BstV1I GCAGC 1 cut(s) 654
BsuI GTATCC 1 cut(s) 125
BsuRI GGCC 3 cut(s) 264, 270, 500
BtsCI GGATG 2 cut(s) 196, 742
BtsI GCAGTG 1 cut(s) 764
BtsIMutI CAGTG 1 cut(s) 764
BveI ACCTGC 1 cut(s) 166
Cac8I GCNNGC 2 cut(s) 156, 647
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 3 cut(s) 8, 269, 616
Csp6I GTAC 2 cut(s) 55, 278
CviAII CATG 1 cut(s) 275
CviQI GTAC 2 cut(s) 55, 278
DdeI CTNAG 3 cut(s) 21, 579, 730
DpnI GATC 2 cut(s) 507, 696
DpnII GATC 2 cut(s) 505, 694
Eam1104I CTCTTC 2 cut(s) 515, 521
EarI CTCTTC 2 cut(s) 515, 521
EciI GGCGGA 1 cut(s) 614
Eco130I CCWWGG 1 cut(s) 265
Eco147I AGGCCT 1 cut(s) 264
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 2 cut(s) 8, 616
Eco88I CYCGRG 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoO109I RGGNCCY 3 cut(s) 8, 269, 616
EcoRI GAATTC 1 cut(s) 671
EcoRV GATATC 1 cut(s) 493
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 1 cut(s) 278
FaqI GGGAC 1 cut(s) 21
FatI CATG 1 cut(s) 274
Fnu4HI GCNGC 2 cut(s) 409, 643
FokI GGATG 2 cut(s) 203, 749
Fsp4HI GCNGC 2 cut(s) 409, 643
FspBI CTAG 2 cut(s) 260, 266
GlaI GCGC 1 cut(s) 248
GluI GCNGC 2 cut(s) 409, 643
GsaI CCCAGC 1 cut(s) 79
HaeIII GGCC 3 cut(s) 264, 270, 500
HhaI GCGC 1 cut(s) 249
Hin1I GRCGYC 1 cut(s) 656
Hin1II CATG 1 cut(s) 278
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 4 cut(s) 239, 427, 564, 683
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 7 cut(s) 289, 340, 460, 525, 580, 677, 733
Hpy188III TCNNGA 2 cut(s) 15, 395
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 1 cut(s) 704
HpyAV CCTTC 2 cut(s) 21, 607
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 1 cut(s) 656
HpyCH4V TGCA 6 cut(s) 92, 194, 447, 480, 649, 757
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 89
HpyF3I CTNAG 3 cut(s) 21, 579, 730
HpySE526I ACGT 1 cut(s) 656
Hsp92I GRCGYC 1 cut(s) 656
Hsp92II CATG 1 cut(s) 278
HspAI GCGC 1 cut(s) 247
Kzo9I GATC 2 cut(s) 505, 694
LmnI GCTCC 4 cut(s) 44, 373, 421, 585
LpnPI CCDG 7 cut(s) 61, 123, 161, 387, 523, 619, 659
Lsp1109I GCAGC 1 cut(s) 654
LweI GCATC 2 cut(s) 181, 451
MaeI CTAG 2 cut(s) 260, 266
MaeII ACGT 1 cut(s) 656
MalI GATC 2 cut(s) 507, 696
MboI GATC 2 cut(s) 505, 694
MboII GAAGA 4 cut(s) 116, 322, 502, 538
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 4 cut(s) 390, 414, 519, 671
MlyI GAGTC 1 cut(s) 573
MmeI TCCRAC 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 390
MseI TTAA 3 cut(s) 404, 621, 781
MwoI GCNNNNNNNGC 2 cut(s) 72, 89
NdeII GATC 2 cut(s) 505, 694
NlaIII CATG 1 cut(s) 278
NlaIV GGNNCC 3 cut(s) 9, 40, 82
PaeR7I CTCGAG 1 cut(s) 148
PceI AGGCCT 1 cut(s) 264
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 3 cut(s) 239, 427, 683
PkrI GCNGC 2 cut(s) 410, 644
PleI GAGTC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 572
PpuMI RGGWCCY 2 cut(s) 8, 616
Psp5II RGGWCCY 2 cut(s) 8, 616
PspFI CCCAGC 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 9, 40, 82
PspPI GGNCC 3 cut(s) 8, 269, 616
PspPPI RGGWCCY 2 cut(s) 8, 616
PspXI VCTCGAGB 1 cut(s) 148
RsaI GTAC 2 cut(s) 56, 279
RsaNI GTAC 2 cut(s) 55, 278
SaqAI TTAA 3 cut(s) 404, 621, 781
SatI GCNGC 2 cut(s) 409, 643
Sau3AI GATC 2 cut(s) 505, 694
Sau96I GGNCC 3 cut(s) 8, 269, 616
SchI GAGTC 1 cut(s) 573
SduI GDGCHC 1 cut(s) 160
SfaNI GCATC 2 cut(s) 181, 451
Sfr274I CTCGAG 1 cut(s) 148
SinI GGWCC 2 cut(s) 8, 616
SlaI CTCGAG 1 cut(s) 148
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 4 cut(s) 390, 414, 519, 671
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 2 cut(s) 408, 599
SspMI CTAG 2 cut(s) 260, 266
StuI AGGCCT 1 cut(s) 264
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 1 cut(s) 659
TaqI TCGA 3 cut(s) 127, 149, 430
TasI AATT 4 cut(s) 390, 414, 519, 671
TatI WGTACW 1 cut(s) 54
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 3 cut(s) 239, 427, 683
Tru1I TTAA 3 cut(s) 404, 621, 781
Tru9I TTAA 3 cut(s) 404, 621, 781
TscAI CASTG 1 cut(s) 764
TseI GCWGC 1 cut(s) 642
TspDTI ATGAA 2 cut(s) 564, 737
TspGWI ACGGA 1 cut(s) 683
TspRI CASTG 1 cut(s) 764
VpaK11BI GGWCC 2 cut(s) 8, 616
XagI CCTNNNNNAGG 1 cut(s) 623
XapI RAATTY 2 cut(s) 390, 671
XhoI CTCGAG 1 cut(s) 148
XmaJI CCTAGG 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 2 cut(s) 260, 266
ZraI GACGTC 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.