Rh4AG176700

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001330
Physical Location & Seq
Reverse (-)
44269219 .. 44272353
3135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG176700.1

Sequence Viewer

Length: 549 bp
ATGGTGGGACCCGCGAGGCCTCAGTTTGTTCTTTTCGGATCCTCCATAGTCCAGCTCAGCTTCAGCCACCGCGGCTGGGGCTCCATTCTCTCCGACGTCTACGCTCGCAAAGCAGACATATTGCTGCGTGGTTACTTTGGATGGAATTCACGGCGTGCTCTAGAGGTGCTTGATCAAGTTTTTCCCAAGGATGCAGCTGTGCAACCTTCTTTGTTGATAGTCTATTTTGGCGGTAATGATTCCATGGGGCCTCATCCATCTGGGCTAGGATCTCATGTACCACTTCCTGAGTATGTTGAGAACATGAGGAAGATTGCGAGTCATCTCCAGAGCCTTTCAGATTCAACGCGCATCATTTTTCTTAGTTGTCCTCCTGTGAATGAGGCCGCAATTCGTGCAAATAAAAGTCCCTATCTCAGTGAGTTAGTAAGAACGAATGAGCTATGCCAACAATATTCAGAAGCTTGTATAAAGCTATGCCAGGAAATGGATATCAAGGTGGTTGATCTTTTTACGGCAATACAGAAAGGAGATAACTGTTTTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

182

Amino Acids

20.21

Weight (kDa)

6.89

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 177 1e-15 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 179 1e-17 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
AccB7I CCANNNNNTGG 1 cut(s) 487
AccI GTMKAC 1 cut(s) 99
AccII CGCG 3 cut(s) 14, 72, 349
AciI CCGC 5 cut(s) 12, 70, 72, 231, 387
AclWI GGATC 3 cut(s) 33, 46, 277
AcsI RAATTY 1 cut(s) 145
AcuI CTGAAG 1 cut(s) 46
AcyI GRCGYC 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 155
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 2 cut(s) 76, 487
AgsI TTSAA 1 cut(s) 345
AjnI CCWGG 1 cut(s) 480
AluBI AGCT 6 cut(s) 55, 60, 197, 442, 464, 475
AluI AGCT 6 cut(s) 55, 60, 197, 442, 464, 475
Alw21I GWGCWC 1 cut(s) 160
AlwI GGATC 3 cut(s) 33, 46, 277
AoxI GGCC 3 cut(s) 17, 248, 384
ApeKI GCWGC 2 cut(s) 124, 194
ApoI RAATTY 1 cut(s) 145
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 2 cut(s) 8, 248
AvaII GGWCC 1 cut(s) 8
BamHI GGATCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 2 cut(s) 111, 206
BccI CCATC 2 cut(s) 135, 265
BceAI ACGGC 2 cut(s) 167, 531
BciT130I CCWGG 1 cut(s) 482
BclI TGATCA 1 cut(s) 172
BfaI CTAG 2 cut(s) 161, 266
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 4 cut(s) 73, 125, 195, 387
BlpI GCTNAGC 1 cut(s) 56
BlsI GCNGC 4 cut(s) 74, 126, 196, 388
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 8, 248
BmiI GGNNCC 5 cut(s) 9, 10, 40, 82, 249
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 2 cut(s) 181, 360
BpmI CTGGAG 1 cut(s) 311
Bpu1102I GCTNAGC 1 cut(s) 56
BsaAI YACGTR 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 3 cut(s) 70, 186, 243
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 487
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 3 cut(s) 70, 186, 243
BseGI GGATG 3 cut(s) 146, 196, 253
BseLI CCNNNNNNNGG 2 cut(s) 76, 487
BseMII CTCAG 4 cut(s) 35, 70, 279, 430
BseXI GCAGC 2 cut(s) 111, 206
BseYI CCCAGC 1 cut(s) 75
Bsh1236I CGCG 3 cut(s) 14, 72, 349
BshFI GGCC 3 cut(s) 19, 250, 386
BsiHKAI GWGCWC 1 cut(s) 160
BslFI GGGAC 2 cut(s) 21, 393
BslI CCNNNNNNNGG 2 cut(s) 76, 487
BsmFI GGGAC 2 cut(s) 21, 393
BsnI GGCC 3 cut(s) 19, 250, 386
Bsp1286I GDGCHC 2 cut(s) 83, 160
Bsp143I GATC 4 cut(s) 38, 172, 269, 505
Bsp1720I GCTNAGC 1 cut(s) 56
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 5 cut(s) 12, 70, 72, 231, 387
BspANI GGCC 3 cut(s) 19, 250, 386
BspCNI CTCAG 4 cut(s) 34, 69, 280, 429
BspFNI CGCG 3 cut(s) 14, 72, 349
BspLI GGNNCC 5 cut(s) 9, 10, 40, 82, 249
BspPI GGATC 3 cut(s) 33, 46, 277
BssECI CCNNGG 3 cut(s) 70, 186, 243
BssMI GATC 4 cut(s) 38, 172, 269, 505
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 186, 243
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 1 cut(s) 539
BstACI GRCGYC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 395
BstBAI YACGTR 1 cut(s) 546
BstC8I GCNNGC 2 cut(s) 106, 156
BstDEI CTNAG 5 cut(s) 21, 56, 288, 362, 416
BstDSI CCRYGG 2 cut(s) 70, 243
BstF5I GGATG 3 cut(s) 146, 196, 253
BstFNI CGCG 3 cut(s) 14, 72, 349
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 4 cut(s) 41, 175, 272, 508
BstMBI GATC 4 cut(s) 38, 172, 269, 505
BstMWI GCNNNNNNNGC 4 cut(s) 72, 78, 110, 395
BstNI CCWGG 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 480
BstSNI TACGTA 1 cut(s) 546
BstUI CGCG 3 cut(s) 14, 72, 349
BstV1I GCAGC 2 cut(s) 111, 206
BstX2I RGATCY 2 cut(s) 38, 269
BstYI RGATCY 2 cut(s) 38, 269
BsuRI GGCC 3 cut(s) 19, 250, 386
BtgI CCRYGG 2 cut(s) 70, 243
BtsCI GGATG 3 cut(s) 146, 196, 253
BtsIMutI CAGTG 1 cut(s) 424
Cac8I GCNNGC 2 cut(s) 106, 156
CfoI GCGC 1 cut(s) 351
Cfr13I GGNCC 2 cut(s) 8, 248
Cfr42I CCGCGG 1 cut(s) 73
Csp6I GTAC 1 cut(s) 278
CviAII CATG 3 cut(s) 244, 275, 304
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 5 cut(s) 21, 56, 288, 362, 416
DpnI GATC 4 cut(s) 40, 174, 271, 507
DpnII GATC 4 cut(s) 38, 172, 269, 505
DraIII CACNNNGTG 1 cut(s) 155
Eco105I TACGTA 1 cut(s) 546
Eco130I CCWWGG 2 cut(s) 186, 243
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 83
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 46
EcoO109I RGGNCCY 2 cut(s) 8, 248
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 480
EcoRV GATATC 1 cut(s) 493
EcoT14I CCWWGG 2 cut(s) 186, 243
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 2 cut(s) 186, 243
FaeI CATG 3 cut(s) 247, 278, 307
FaiI YATR 9 cut(s) 47, 119, 245, 276, 294, 305, 445, 470, 478
FaqI GGGAC 2 cut(s) 21, 393
FatI CATG 3 cut(s) 243, 274, 303
FauI CCCGC 1 cut(s) 19
FbaI TGATCA 1 cut(s) 172
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 4 cut(s) 73, 125, 195, 387
FokI GGATG 3 cut(s) 153, 203, 240
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 4 cut(s) 73, 125, 195, 387
FspBI CTAG 2 cut(s) 161, 266
GlaI GCGC 1 cut(s) 350
GluI GCNGC 4 cut(s) 73, 125, 195, 387
GsaI CCCAGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 3 cut(s) 19, 250, 386
HhaI GCGC 1 cut(s) 351
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 3 cut(s) 247, 278, 307
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 3 cut(s) 239, 319, 341
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 4 cut(s) 38, 94, 340, 460
Hpy188III TCNNGA 3 cut(s) 161, 287, 328
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 1 cut(s) 216
HpyCH4III ACNGT 1 cut(s) 539
HpyCH4IV ACGT 2 cut(s) 96, 545
HpyCH4V TGCA 3 cut(s) 194, 202, 398
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 78, 110, 395
HpyF3I CTNAG 5 cut(s) 21, 56, 288, 362, 416
HpySE526I ACGT 2 cut(s) 96, 545
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 3 cut(s) 247, 278, 307
HspAI GCGC 1 cut(s) 349
KflI GGGWCCC 1 cut(s) 8
Ksp22I TGATCA 1 cut(s) 172
KspI CCGCGG 1 cut(s) 73
Kzo9I GATC 4 cut(s) 38, 172, 269, 505
LmnI GCTCC 1 cut(s) 86
LpnPI CCDG 8 cut(s) 61, 65, 246, 300, 341, 387, 467, 494
Lsp1109I GCAGC 2 cut(s) 111, 206
LweI GCATC 2 cut(s) 181, 360
MaeI CTAG 2 cut(s) 161, 266
MaeII ACGT 2 cut(s) 96, 545
MaeIII GTNAC 1 cut(s) 131
MalI GATC 4 cut(s) 40, 174, 271, 507
MboI GATC 4 cut(s) 38, 172, 269, 505
MboII GAAGA 1 cut(s) 322
MflI RGATCY 2 cut(s) 38, 269
MhlI GDGCHC 2 cut(s) 83, 160
MluCI AATT 2 cut(s) 145, 390
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 8 cut(s) 9, 30, 52, 157, 261, 300, 376, 381
MspA1I CMGCKG 2 cut(s) 72, 197
MspR9I CCNGG 1 cut(s) 482
MvaI CCWGG 1 cut(s) 482
MvnI CGCG 3 cut(s) 14, 72, 349
MwoI GCNNNNNNNGC 4 cut(s) 72, 78, 110, 395
NcoI CCATGG 1 cut(s) 243
NdeII GATC 4 cut(s) 38, 172, 269, 505
NlaIII CATG 3 cut(s) 247, 278, 307
NlaIV GGNNCC 5 cut(s) 9, 10, 40, 82, 249
PceI AGGCCT 1 cut(s) 19
PfeI GAWTC 2 cut(s) 239, 341
PflMI CCANNNNNTGG 1 cut(s) 487
PkrI GCNGC 4 cut(s) 74, 126, 196, 388
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
Ppu21I YACGTR 1 cut(s) 546
PpuMI RGGWCCY 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 5 cut(s) 9, 10, 40, 82, 249
PspPI GGNCC 2 cut(s) 8, 248
PspPPI RGGWCCY 1 cut(s) 8
PsuI RGATCY 2 cut(s) 38, 269
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
SacII CCGCGG 1 cut(s) 73
SatI GCNGC 4 cut(s) 73, 125, 195, 387
Sau3AI GATC 4 cut(s) 38, 172, 269, 505
Sau96I GGNCC 2 cut(s) 8, 248
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 2 cut(s) 83, 160
SfaNI GCATC 2 cut(s) 181, 360
Sfr303I CCGCGG 1 cut(s) 73
SgrBI CCGCGG 1 cut(s) 73
SinI GGWCC 1 cut(s) 8
SnaBI TACGTA 1 cut(s) 546
Sse9I AATT 2 cut(s) 145, 390
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 5 cut(s) 12, 70, 72, 231, 387
SspI AATATT 1 cut(s) 455
SspMI CTAG 2 cut(s) 161, 266
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 480
StyI CCWWGG 2 cut(s) 186, 243
TaaI ACNGT 1 cut(s) 539
TaiI ACGT 2 cut(s) 99, 548
TasI AATT 2 cut(s) 145, 390
TauI GCSGC 2 cut(s) 75, 389
TfiI GAWTC 2 cut(s) 239, 341
TscAI CASTG 1 cut(s) 424
TseI GCWGC 2 cut(s) 124, 194
TspRI CASTG 1 cut(s) 424
Van91I CCANNNNNTGG 1 cut(s) 487
VpaK11BI GGWCC 1 cut(s) 8
XapI RAATTY 1 cut(s) 145
XbaI TCTAGA 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 2 cut(s) 161, 266
ZraI GACGTC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.