Rh3DG125400

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001330
Physical Location & Seq
Reverse (-)
10589581 .. 10592064
2484 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG125400.1

Sequence Viewer

Length: 711 bp
ATGGTCGGACCAATAAGACCTCAGTTTGTGCTGTTTGGTTCGTCCATCGTCCAGTTCAGTTTCAGCAATGGAGGTTGGGGCGCCATCCTTGCTGACTTATATTCTCGTAAGGCGGATATACTTGTGCGTGGCTACGCTGGTTGGAATTCAAGGAATGGTCTGCAGGTTTTAGAGCAAGTGTTTCCAAAGGATGCAAGTATCCAGCCATCGTTGGTGATAGTCTATTTTGGTGGTAATGATTCGATGCTGCCGGATCCACTAGGCTTAAGCCTTCACGTACCTCTTCCTGAATATGTAGAAAATATGAGGAAGATTGCTACCCATTTGAAGAGCCTTTCGCAGAAAACCCGCCTTATCTTCCTGACTGCTCCTCCTGTCAACGAGGAACAAATGCGAAAAGCATTTAGTGGAATCATGCGGGAGAATAGTCGAACAAACACGCAGTTGCAAATGTATTCAGATGCTTGTGTGGAGCTGTGCAGAGAGATGGATATCAAGGCAGTTGATCTCTCTACTTCAATGCAGAAAAGAAGTGACTGGTCGACTGTATGCTTTACGGATGGAATCCATTTTTCATCCGAGGGGAGCAAGATCGTGGTGGAGGAGATACTGAAGGTCCTTGAAGAAGCAGACTGGGAACCCAGCCTGCAATGGAACTCATTGCCAACTGAGTTTGAAGAGTTTGCACCTTATACCGCCGCTCGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

236

Amino Acids

26.53

Weight (kDa)

5.18

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 203 4.6e-21 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 197 5.2e-22 GDSL-like Lipase/Acylhydrolase family
DUF459 PF04311 67 - 205 2.1e-07 Protein of unknown function (DUF459)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 154
AccB1I GGYRCC 1 cut(s) 80
AccBSI CCGCTC 1 cut(s) 701
AccI GTMKAC 1 cut(s) 542
AciI CCGC 5 cut(s) 113, 349, 418, 696, 699
AclWI GGATC 2 cut(s) 248, 261
AcsI RAATTY 1 cut(s) 145
AcuI CTGAAG 1 cut(s) 632
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 1 cut(s) 702
AflII CTTAAG 1 cut(s) 265
AgsI TTSAA 5 cut(s) 150, 328, 519, 623, 677
AluBI AGCT 1 cut(s) 475
AluI AGCT 1 cut(s) 475
AlwI GGATC 2 cut(s) 248, 261
Ama87I CYCGRG 1 cut(s) 702
ApeKI GCWGC 1 cut(s) 247
ApoI RAATTY 1 cut(s) 145
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 8, 616
AsuHPI GGTGA 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 2 cut(s) 8, 616
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 234
BccI CCATC 5 cut(s) 53, 92, 214, 481, 554
BciVI GTATCC 1 cut(s) 209
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 161
BfoI RGCGCY 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 265
BfuAI ACCTGC 1 cut(s) 154
BfuI GTATCC 1 cut(s) 209
BisI GCNGC 2 cut(s) 248, 699
BlsI GCNGC 2 cut(s) 249, 700
Bme18I GGWCC 2 cut(s) 8, 616
BmeT110I CYCGRG 1 cut(s) 702
BmgT120I GGNCC 2 cut(s) 8, 616
BmiI GGNNCC 3 cut(s) 82, 255, 639
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 3 cut(s) 181, 234, 451
BmuI ACTGGG 1 cut(s) 643
BsaAI YACGTR 1 cut(s) 277
BsaBI GATNNNNATC 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 579
BsaXI ACNNNNNCTCC 2 cut(s) 355, 385
Bsc4I CCNNNNNNNGG 1 cut(s) 702
Bse1I ACTGG 3 cut(s) 52, 542, 638
Bse3DI GCAATG 3 cut(s) 73, 656, 659
Bse8I GATNNNNATC 1 cut(s) 491
BseDI CCNNGG 1 cut(s) 579
BseGI GGATG 4 cut(s) 84, 196, 565, 575
BseJI GATNNNNATC 1 cut(s) 491
BseLI CCNNNNNNNGG 1 cut(s) 702
BseMI GCAATG 3 cut(s) 73, 656, 659
BseMII CTCAG 2 cut(s) 35, 660
BseNI ACTGG 3 cut(s) 52, 542, 638
BseRI GAGGAG 2 cut(s) 360, 617
BseXI GCAGC 1 cut(s) 234
BseYI CCCAGC 1 cut(s) 641
BsgI GTGCAG 1 cut(s) 499
BshNI GGYRCC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 702
BsoBI CYCGRG 1 cut(s) 702
Bsp143I GATC 3 cut(s) 253, 505, 591
BspACI CCGC 5 cut(s) 113, 349, 418, 696, 699
BspCNI CTCAG 2 cut(s) 34, 661
BspLI GGNNCC 3 cut(s) 82, 255, 639
BspMAI CTGCAG 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 154
BspPI GGATC 2 cut(s) 248, 261
BspQI GCTCTTC 1 cut(s) 323
BspT107I GGYRCC 1 cut(s) 80
BspTI CTTAAG 1 cut(s) 265
BsrBI CCGCTC 1 cut(s) 701
BsrDI GCAATG 3 cut(s) 73, 656, 659
BsrI ACTGG 3 cut(s) 52, 542, 638
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 3 cut(s) 253, 505, 591
BssNI GRCGYC 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 547
Bst6I CTCTTC 3 cut(s) 288, 323, 672
BstACI GRCGYC 1 cut(s) 81
BstAFI CTTAAG 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 277
BstC8I GCNNGC 1 cut(s) 647
BstDEI CTNAG 2 cut(s) 21, 669
BstF5I GGATG 4 cut(s) 84, 196, 565, 575
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 3 cut(s) 256, 508, 594
BstMBI GATC 3 cut(s) 253, 505, 591
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstSFI CTRYAG 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 234
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuI GTATCC 1 cut(s) 209
BtsCI GGATG 4 cut(s) 84, 196, 565, 575
BveI ACCTGC 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 647
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 8, 616
Csp6I GTAC 1 cut(s) 278
CviAII CATG 1 cut(s) 415
CviJI RGCY 7 cut(s) 132, 205, 264, 270, 333, 475, 645
CviKI_1 RGCY 7 cut(s) 132, 205, 264, 270, 333, 475, 645
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 2 cut(s) 21, 669
DinI GGCGCC 1 cut(s) 82
DpnI GATC 3 cut(s) 255, 507, 593
DpnII GATC 3 cut(s) 253, 505, 591
Eam1104I CTCTTC 3 cut(s) 288, 323, 672
EarI CTCTTC 3 cut(s) 288, 323, 672
EciI GGCGGA 1 cut(s) 128
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 2 cut(s) 8, 616
Eco57I CTGAAG 1 cut(s) 632
Eco88I CYCGRG 1 cut(s) 702
EcoO109I RGGNCCY 1 cut(s) 616
EcoRI GAATTC 1 cut(s) 145
EcoRV GATATC 1 cut(s) 493
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
FaeI CATG 1 cut(s) 418
FaiI YATR 7 cut(s) 100, 119, 294, 305, 416, 550, 693
FatI CATG 1 cut(s) 414
FauI CCCGC 2 cut(s) 356, 411
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 2 cut(s) 248, 699
FokI GGATG 4 cut(s) 71, 203, 562, 572
Fsp4HI GCNGC 2 cut(s) 248, 699
FspBI CTAG 1 cut(s) 260
GlaI GCGC 1 cut(s) 82
GluI GCNGC 2 cut(s) 248, 699
GsaI CCCAGC 1 cut(s) 645
HaeII RGCGCY 1 cut(s) 84
HapII CCGG 1 cut(s) 251
HhaI GCGC 1 cut(s) 83
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 1 cut(s) 418
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 2 cut(s) 379, 543
HindII GTYRAC 2 cut(s) 379, 543
HinfI GANTC 3 cut(s) 239, 411, 564
HpaII CCGG 1 cut(s) 251
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 2 cut(s) 379, 543
Hpy188I TCNGA 3 cut(s) 8, 460, 580
Hpy188III TCNNGA 2 cut(s) 287, 361
Hpy8I GTNNAC 2 cut(s) 379, 543
HpyAV CCTTC 2 cut(s) 281, 607
HpyCH4III ACNGT 1 cut(s) 547
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 7 cut(s) 163, 194, 448, 480, 523, 649, 686
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 2 cut(s) 21, 669
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 1 cut(s) 418
HspAI GCGC 1 cut(s) 81
KasI GGCGCC 1 cut(s) 80
Kzo9I GATC 3 cut(s) 253, 505, 591
LguI GCTCTTC 1 cut(s) 323
LmnI GCTCC 3 cut(s) 373, 472, 585
Lsp1109I GCAGC 1 cut(s) 234
LweI GCATC 3 cut(s) 181, 234, 451
MaeI CTAG 1 cut(s) 260
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 533
MalI GATC 3 cut(s) 255, 507, 593
MbiI CCGCTC 1 cut(s) 701
MboI GATC 3 cut(s) 253, 505, 591
MboII GAAGA 6 cut(s) 275, 322, 340, 349, 635, 689
MflI RGATCY 1 cut(s) 253
MluCI AATT 1 cut(s) 145
Mly113I GGCGCC 1 cut(s) 81
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 8 cut(s) 30, 65, 291, 300, 376, 381, 574, 595
MseI TTAA 1 cut(s) 266
MspCI CTTAAG 1 cut(s) 265
MspI CCGG 1 cut(s) 251
MwoI GCNNNNNNNGC 1 cut(s) 89
NarI GGCGCC 1 cut(s) 81
NdeII GATC 3 cut(s) 253, 505, 591
NlaIII CATG 1 cut(s) 418
NlaIV GGNNCC 3 cut(s) 82, 255, 639
NmuCI GTSAC 1 cut(s) 533
PciSI GCTCTTC 1 cut(s) 323
PfeI GAWTC 3 cut(s) 239, 411, 564
PkrI GCNGC 2 cut(s) 249, 700
PluTI GGCGCC 1 cut(s) 84
Ppu21I YACGTR 1 cut(s) 277
PpuMI RGGWCCY 1 cut(s) 616
Psp5II RGGWCCY 1 cut(s) 616
PspFI CCCAGC 1 cut(s) 641
PspN4I GGNNCC 3 cut(s) 82, 255, 639
PspPI GGNCC 2 cut(s) 8, 616
PspPPI RGGWCCY 1 cut(s) 616
PstI CTGCAG 1 cut(s) 165
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
SalI GTCGAC 1 cut(s) 541
SapI GCTCTTC 1 cut(s) 323
SaqAI TTAA 1 cut(s) 266
SatI GCNGC 2 cut(s) 248, 699
Sau3AI GATC 3 cut(s) 253, 505, 591
Sau96I GGNCC 2 cut(s) 8, 616
SetI ASST 8 cut(s) 22, 76, 168, 279, 283, 477, 618, 691
SfaNI GCATC 3 cut(s) 181, 234, 451
SfcI CTRYAG 1 cut(s) 161
SfoI GGCGCC 1 cut(s) 82
SinI GGWCC 2 cut(s) 8, 616
SmlI CTYRAG 1 cut(s) 265
SmoI CTYRAG 1 cut(s) 265
Sse9I AATT 1 cut(s) 145
SsiI CCGC 5 cut(s) 113, 349, 418, 696, 699
SspDI GGCGCC 1 cut(s) 80
SspMI CTAG 1 cut(s) 260
TaaI ACNGT 1 cut(s) 547
TaiI ACGT 1 cut(s) 279
TaqI TCGA 3 cut(s) 242, 430, 542
TasI AATT 1 cut(s) 145
TauI GCSGC 1 cut(s) 701
TfiI GAWTC 3 cut(s) 239, 411, 564
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TseFI GTSAC 1 cut(s) 533
TseI GCWGC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 533
TspDTI ATGAA 1 cut(s) 564
TspGWI ACGGA 1 cut(s) 572
Vha464I CTTAAG 1 cut(s) 265
VpaK11BI GGWCC 2 cut(s) 8, 616
XapI RAATTY 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 542
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.