Rorug01G0354200

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001330
Physical Location & Seq
Reverse (-)
47090238 .. 47091449
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0354200.1

Sequence Viewer

Length: 981 bp
ATGAACACCCTCCGATCATTCCCAAATCAGACACCACAAAATTGCTATGCTTTGCCTGTCAATAAGCAAACCGTACGCTACCTGATTCGGGTTGGGTTCTACTATGGCAACTATGATGGTCTCTCGAACCCTCCATCGTTCGATATTCATATCGATGGTCAAAAATGGTCGACGGTCAAAACATCAACTCTGGAAGAAGGGCCAATGTATCATGAAGCCTTGTATGAAACTCAAGGGTCAGGTTCTATTAGTCTTTGCGTTGTGCAAGTTAAGGATGGAGGGGTTCCCTTTATTTCTTCAATAGAAGCTGTTCCGTTATCGGCTCCTTTTAATCCATTGTACCCTAAGATGGAGACAAACAACACTTTCGATTTAGTATCCAGGATAAACTTTGCTGGTGATGAAATAAGGTTCACAGGGTTATTATCAGAAAACTATAACCGAATATGGACACGAGGAACAACTCCGCCAAACTGCAACGAAGTTTCTACCTTACCAGATATACTCTCCCCAGAAAATGACCCTCCTATTTCAGTGTTGCGCACCGCCATAGAATCAATAGACCTTACCCATCCAATAACCTTGTCCGTTAGCCTCTCGCAAACAACCCCTCAGTCAGCTTACTTTGTGCTCTATTTTACCGAAACGACAAGCCTACCTAAACCAGGCGACACCAGAATCATTCAGATCAACATCAATGGTCAAATGAAATCCACAGTGACACTTGAGTTCACAAAATGCAAGGTGATCACATTATACCCTGTGATCGTCGAGGGTACCACAATTAACGTGACATTGGCCTCGGCTAGTAGCTCCACCCTACCTCCGATCATCAGTGCAATGGAAGTGTTCACAAGAGTGGATCATGAAAAGAGTTCATCTCCTCCTCCTCCTTCTCCAGCCTCACATGGAACTAGAGGAACTTCTTTTGTGCGCACATTAATATCGTTTCTTGTGTTTGTTTGTTTCTTCTATTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

326

Amino Acids

35.99

Weight (kDa)

5.57

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 2 - 285 1.3e-55 Malectin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 542, 935
Acc65I GGTACC 1 cut(s) 776
AccB1I GGYRCC 1 cut(s) 776
AccI GTMKAC 1 cut(s) 170
AciI CCGC 2 cut(s) 467, 546
AclWI GGATC 1 cut(s) 870
AfaI GTAC 3 cut(s) 75, 341, 778
AfiI CCNNNNNNNGG 3 cut(s) 88, 349, 665
AgsI TTSAA 1 cut(s) 300
AjnI CCWGG 2 cut(s) 380, 664
AleI CACNNNNGTG 1 cut(s) 857
AluBI AGCT 3 cut(s) 308, 620, 813
AluI AGCT 3 cut(s) 308, 620, 813
Alw21I GWGCWC 1 cut(s) 633
Alw26I GTCTC 2 cut(s) 125, 347
AlwI GGATC 1 cut(s) 870
AoxI GGCC 2 cut(s) 200, 798
AseI ATTAAT 1 cut(s) 941
Asp700I GAANNNNTTC 1 cut(s) 309
Asp718I GGTACC 1 cut(s) 776
AspLEI GCGC 2 cut(s) 543, 936
AspS9I GGNCC 1 cut(s) 200
AsuHPI GGTGA 2 cut(s) 410, 757
BanI GGYRCC 1 cut(s) 776
BauI CACGAG 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 633
BccI CCATC 6 cut(s) 110, 142, 149, 269, 343, 579
BciT130I CCWGG 2 cut(s) 382, 666
BciVI GTATCC 1 cut(s) 388
BclI TGATCA 1 cut(s) 747
BcoDI GTCTC 2 cut(s) 125, 347
BfaI CTAG 3 cut(s) 807, 915, 979
BfuI GTATCC 1 cut(s) 388
Bme1390I CCNGG 2 cut(s) 382, 666
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 3 cut(s) 285, 324, 778
BmrFI CCNGG 2 cut(s) 382, 666
BplI GAGNNNNNCTC 2 cut(s) 865, 897
BpmI CTGGAG 1 cut(s) 882
BpuEI CTTGAG 2 cut(s) 216, 746
Bsa29I ATCGAT 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 692
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 801
BsaXI ACNNNNNCTCC 2 cut(s) 808, 838
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 349, 665
Bse3DI GCAATG 1 cut(s) 846
Bse8I GATNNNNATC 1 cut(s) 692
BseBI CCWGG 2 cut(s) 382, 666
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 801
BseGI GGATG 2 cut(s) 280, 571
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 3 cut(s) 88, 349, 665
BseMI GCAATG 1 cut(s) 846
BseMII CTCAG 1 cut(s) 626
BseRI GAGGAG 3 cut(s) 873, 876, 879
BshFI GGCC 2 cut(s) 202, 800
BshNI GGYRCC 1 cut(s) 776
BshVI ATCGAT 1 cut(s) 153
BsiHKAI GWGCWC 1 cut(s) 633
BsiWI CGTACG 1 cut(s) 73
BslI CCNNNNNNNGG 3 cut(s) 88, 349, 665
BsmAI GTCTC 2 cut(s) 125, 347
BsnI GGCC 2 cut(s) 202, 800
Bso31I GGTCTC 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 633
Bsp143I GATC 6 cut(s) 14, 687, 747, 765, 828, 862
BspACI CCGC 2 cut(s) 467, 546
BspANI GGCC 2 cut(s) 202, 800
BspCNI CTCAG 1 cut(s) 625
BspDI ATCGAT 1 cut(s) 153
BspHI TCATGA 2 cut(s) 211, 865
BspLI GGNNCC 3 cut(s) 285, 324, 778
BspPI GGATC 1 cut(s) 870
BspT107I GGYRCC 1 cut(s) 776
BspTNI GGTCTC 1 cut(s) 125
BsrDI GCAATG 1 cut(s) 846
BssECI CCNNGG 1 cut(s) 801
BssMI GATC 6 cut(s) 14, 687, 747, 765, 828, 862
BssSI CACGAG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 2 cut(s) 382, 666
Bst4CI ACNGT 3 cut(s) 73, 175, 718
BstDEI CTNAG 2 cut(s) 345, 612
BstENI CCTNNNNNAGG 1 cut(s) 663
BstF5I GGATG 2 cut(s) 280, 571
BstHHI GCGC 2 cut(s) 543, 936
BstKTI GATC 6 cut(s) 17, 690, 750, 768, 831, 865
BstMAI GTCTC 2 cut(s) 125, 347
BstMBI GATC 6 cut(s) 14, 687, 747, 765, 828, 862
BstNI CCWGG 2 cut(s) 382, 666
BstSCI CCNGG 2 cut(s) 380, 664
Bsu15I ATCGAT 1 cut(s) 153
BsuI GTATCC 1 cut(s) 388
BsuRI GGCC 2 cut(s) 202, 800
BsuTUI ATCGAT 1 cut(s) 153
BtsCI GGATG 2 cut(s) 280, 571
BtsIMutI CAGTG 3 cut(s) 540, 723, 841
CciI TCATGA 2 cut(s) 211, 865
CfoI GCGC 2 cut(s) 543, 936
Cfr13I GGNCC 1 cut(s) 200
ClaI ATCGAT 1 cut(s) 153
Csp6I GTAC 3 cut(s) 74, 340, 777
CviAII CATG 3 cut(s) 212, 866, 908
CviQI GTAC 3 cut(s) 74, 340, 777
DdeI CTNAG 2 cut(s) 345, 612
DpnI GATC 6 cut(s) 16, 689, 749, 767, 830, 864
DpnII GATC 6 cut(s) 14, 687, 747, 765, 828, 862
EciI GGCGGA 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 125
EcoNI CCTNNNNNAGG 1 cut(s) 663
EcoRII CCWGG 2 cut(s) 380, 664
FaeI CATG 3 cut(s) 215, 869, 911
FatI CATG 3 cut(s) 211, 865, 907
FbaI TGATCA 1 cut(s) 747
FblI GTMKAC 1 cut(s) 170
FokI GGATG 2 cut(s) 287, 558
FspAI RTGCGCAY 1 cut(s) 935
FspBI CTAG 3 cut(s) 807, 915, 979
FspI TGCGCA 2 cut(s) 542, 935
GlaI GCGC 2 cut(s) 542, 935
GsuI CTGGAG 1 cut(s) 882
HaeIII GGCC 2 cut(s) 202, 800
HhaI GCGC 2 cut(s) 543, 936
Hin1II CATG 3 cut(s) 215, 869, 911
Hin6I GCGC 2 cut(s) 541, 934
HinP1I GCGC 2 cut(s) 541, 934
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HinfI GANTC 3 cut(s) 85, 554, 678
HphI GGTGA 2 cut(s) 410, 757
Hpy166II GTNNAC 4 cut(s) 171, 414, 732, 852
Hpy188I TCNGA 5 cut(s) 14, 30, 430, 687, 828
Hpy188III TCNNGA 4 cut(s) 124, 191, 212, 866
Hpy8I GTNNAC 4 cut(s) 171, 414, 732, 852
Hpy99I CGWCG 2 cut(s) 175, 773
HpyAV CCTTC 2 cut(s) 191, 903
HpyCH4III ACNGT 3 cut(s) 73, 175, 718
HpyCH4IV ACGT 1 cut(s) 789
HpyCH4V TGCA 4 cut(s) 265, 477, 741, 839
HpyF3I CTNAG 2 cut(s) 345, 612
HpySE526I ACGT 1 cut(s) 789
Hsp92II CATG 3 cut(s) 215, 869, 911
HspAI GCGC 2 cut(s) 541, 934
KpnI GGTACC 1 cut(s) 780
Ksp22I TGATCA 1 cut(s) 747
Kzo9I GATC 6 cut(s) 14, 687, 747, 765, 828, 862
LmnI GCTCC 2 cut(s) 328, 818
MaeI CTAG 3 cut(s) 807, 915, 979
MaeII ACGT 1 cut(s) 789
MaeIII GTNAC 2 cut(s) 718, 790
MalI GATC 6 cut(s) 16, 689, 749, 767, 830, 864
MboI GATC 6 cut(s) 14, 687, 747, 765, 828, 862
MboII GAAGA 3 cut(s) 206, 288, 961
MhlI GDGCHC 1 cut(s) 633
MluCI AATT 2 cut(s) 40, 783
MroXI GAANNNNTTC 1 cut(s) 309
MseI TTAA 4 cut(s) 270, 330, 786, 941
MslI CAYNNNNRTG 2 cut(s) 153, 857
MspR9I CCNGG 2 cut(s) 382, 666
MvaI CCWGG 2 cut(s) 382, 666
NdeII GATC 6 cut(s) 14, 687, 747, 765, 828, 862
NlaIII CATG 3 cut(s) 215, 869, 911
NlaIV GGNNCC 3 cut(s) 285, 324, 778
NmeAIII GCCGAG 1 cut(s) 782
NmuCI GTSAC 2 cut(s) 718, 790
NsbI TGCGCA 2 cut(s) 542, 935
OliI CACNNNNGTG 1 cut(s) 857
PagI TCATGA 2 cut(s) 211, 865
PdmI GAANNNNTTC 1 cut(s) 309
PfeI GAWTC 3 cut(s) 85, 554, 678
Pfl23II CGTACG 1 cut(s) 73
PfoI TCCNGGA 1 cut(s) 380
PshBI ATTAAT 1 cut(s) 941
Psp6I CCWGG 2 cut(s) 380, 664
PspGI CCWGG 2 cut(s) 380, 664
PspLI CGTACG 1 cut(s) 73
PspN4I GGNNCC 3 cut(s) 285, 324, 778
PspPI GGNCC 1 cut(s) 200
RsaI GTAC 3 cut(s) 75, 341, 778
RsaNI GTAC 3 cut(s) 74, 340, 777
RseI CAYNNNNRTG 2 cut(s) 153, 857
SalI GTCGAC 1 cut(s) 169
SaqAI TTAA 4 cut(s) 270, 330, 786, 941
Sau3AI GATC 6 cut(s) 14, 687, 747, 765, 828, 862
Sau96I GGNCC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 382, 666
SduI GDGCHC 1 cut(s) 633
SmiMI CAYNNNNRTG 2 cut(s) 153, 857
SmlI CTYRAG 2 cut(s) 231, 725
SmoI CTYRAG 2 cut(s) 231, 725
Sse9I AATT 2 cut(s) 40, 783
SsiI CCGC 2 cut(s) 467, 546
SspMI CTAG 3 cut(s) 807, 915, 979
StyD4I CCNGG 2 cut(s) 380, 664
TaaI ACNGT 3 cut(s) 73, 175, 718
TaiI ACGT 1 cut(s) 792
TaqI TCGA 6 cut(s) 125, 141, 153, 170, 369, 771
TasI AATT 2 cut(s) 40, 783
TfiI GAWTC 3 cut(s) 85, 554, 678
Tru1I TTAA 4 cut(s) 270, 330, 786, 941
Tru9I TTAA 4 cut(s) 270, 330, 786, 941
TscAI CASTG 3 cut(s) 540, 723, 841
TseFI GTSAC 2 cut(s) 718, 790
Tsp45I GTSAC 2 cut(s) 718, 790
TspDTI ATGAA 8 cut(s) 17, 137, 228, 240, 417, 722, 867, 882
TspGWI ACGGA 2 cut(s) 303, 577
TspRI CASTG 3 cut(s) 540, 723, 841
VspI ATTAAT 1 cut(s) 941
XagI CCTNNNNNAGG 1 cut(s) 663
XmiI GTMKAC 1 cut(s) 170
XmnI GAANNNNTTC 1 cut(s) 309
XspI CTAG 3 cut(s) 807, 915, 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.