Rh4CG187700

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001330
Physical Location & Seq
Reverse (-)
40490393 .. 40492924
2532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG187700.1

Sequence Viewer

Length: 756 bp
ATGGTGGGACCCGCGAGGCCTCAGTTTGTTCTATTCGGATCCTCCATAGTCCAGCTCAGCTTCAGCCACCGCGGCTGGGGCTCCATTCTCTCCGACGTCTACGCCCGCAAAGCAGACATATTGCTGCGTGGTTACTTTGGATGGAATTCACGGCGTGCTCTAGAGGTGCTTGATCAAGTTTTTCCCAAGGATGCAGCTGTGCAACCTTCTTTGTTGATAGTCTATTTTGGCGGTAATGATTCGATGGGGCCTCATCCATCTGGGCTAGGATCTCATGTACCACTTCCTGAGTATGTTGAGAACATGAGGAAGATTGCGAGTCATCTCCAGAGCCTTTCAGATTCAACGCGCATCATTTTTCTTAGTTGTCCTCCTGTGAATGAGGCCGCAATTCGTGCAAATAAAAGTCCCTATCTCAGTGAGTTAGTAAGAACGAATGAGCTATGCCAACAATATTCAGAAGCTTGTATAAAGCTATGCCAGGAAATGGATATCAAGGTGGTTGATCTTTTTACGGCAATACAGAAAGGAGATAACTGTTTTACAGATGGGATTCATTTATCAGCTGAAGGGAGCAAAATAGTGGCGGAGGAGATAGTGAAAGTACTGAGAGAAGCTGACTGGAAGCCAAGTTTGCACTGGAAATCCATGCCAACAGAATTTGCAGAGGATTCACCATATTATCCTGTTGCAGCTGATGGAAAGACGCCGATAAATCCCTCTGAATGGACGTTTTATAGAGAGTTTCACATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

251

Amino Acids

28.11

Weight (kDa)

5.81

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 199 7.2e-22 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 193 4.2e-21 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
AccB7I CCANNNNNTGG 1 cut(s) 487
AccI GTMKAC 1 cut(s) 99
AccII CGCG 3 cut(s) 14, 72, 349
AciI CCGC 7 cut(s) 12, 70, 72, 106, 231, 387, 587
AclWI GGATC 3 cut(s) 33, 46, 277
AcsI RAATTY 2 cut(s) 145, 659
AcuI CTGAAG 2 cut(s) 46, 588
AcyI GRCGYC 2 cut(s) 96, 707
AdeI CACNNNGTG 1 cut(s) 155
AfaI GTAC 2 cut(s) 279, 606
AfiI CCNNNNNNNGG 3 cut(s) 76, 487, 726
AgsI TTSAA 1 cut(s) 345
AjnI CCWGG 1 cut(s) 480
AluBI AGCT 9 cut(s) 55, 60, 197, 442, 464, 475, 566, 617, 695
AluI AGCT 9 cut(s) 55, 60, 197, 442, 464, 475, 566, 617, 695
Alw21I GWGCWC 1 cut(s) 160
AlwI GGATC 3 cut(s) 33, 46, 277
AoxI GGCC 3 cut(s) 17, 248, 384
ApeKI GCWGC 3 cut(s) 124, 194, 692
ApoI RAATTY 2 cut(s) 145, 659
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 2 cut(s) 8, 248
AsuHPI GGTGA 1 cut(s) 666
AvaII GGWCC 1 cut(s) 8
BamHI GGATCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 3 cut(s) 111, 206, 704
BccI CCATC 5 cut(s) 135, 238, 265, 542, 692
BceAI ACGGC 2 cut(s) 167, 531
BciT130I CCWGG 1 cut(s) 482
BclI TGATCA 1 cut(s) 172
BfaI CTAG 2 cut(s) 161, 266
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 5 cut(s) 73, 125, 195, 387, 693
BlpI GCTNAGC 1 cut(s) 56
BlsI GCNGC 5 cut(s) 74, 126, 196, 388, 694
BmcAI AGTACT 1 cut(s) 606
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 8, 248
BmiI GGNNCC 5 cut(s) 9, 10, 40, 82, 249
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 2 cut(s) 181, 360
BpmI CTGGAG 1 cut(s) 311
Bpu1102I GCTNAGC 1 cut(s) 56
BsaHI GRCGYC 2 cut(s) 96, 707
BsaJI CCNNGG 2 cut(s) 70, 186
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 487, 726
Bse1I ACTGG 2 cut(s) 626, 644
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 2 cut(s) 70, 186
BseGI GGATG 3 cut(s) 146, 196, 253
BseLI CCNNNNNNNGG 3 cut(s) 76, 487, 726
BseMII CTCAG 5 cut(s) 35, 70, 279, 430, 599
BseNI ACTGG 2 cut(s) 626, 644
BseRI GAGGAG 1 cut(s) 605
BseXI GCAGC 3 cut(s) 111, 206, 704
BseYI CCCAGC 1 cut(s) 75
Bsh1236I CGCG 3 cut(s) 14, 72, 349
BshFI GGCC 3 cut(s) 19, 250, 386
BsiHKAI GWGCWC 1 cut(s) 160
BslFI GGGAC 2 cut(s) 21, 393
BslI CCNNNNNNNGG 3 cut(s) 76, 487, 726
BsmFI GGGAC 2 cut(s) 21, 393
BsnI GGCC 3 cut(s) 19, 250, 386
Bsp1286I GDGCHC 2 cut(s) 83, 160
Bsp143I GATC 4 cut(s) 38, 172, 269, 505
Bsp1720I GCTNAGC 1 cut(s) 56
BspACI CCGC 7 cut(s) 12, 70, 72, 106, 231, 387, 587
BspANI GGCC 3 cut(s) 19, 250, 386
BspCNI CTCAG 5 cut(s) 34, 69, 280, 429, 600
BspFNI CGCG 3 cut(s) 14, 72, 349
BspLI GGNNCC 5 cut(s) 9, 10, 40, 82, 249
BspPI GGATC 3 cut(s) 33, 46, 277
BsrI ACTGG 2 cut(s) 626, 644
BssECI CCNNGG 2 cut(s) 70, 186
BssMI GATC 4 cut(s) 38, 172, 269, 505
BssNI GRCGYC 2 cut(s) 96, 707
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 1 cut(s) 539
BstACI GRCGYC 2 cut(s) 96, 707
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 2 cut(s) 106, 156
BstDEI CTNAG 6 cut(s) 21, 56, 288, 362, 416, 608
BstDSI CCRYGG 1 cut(s) 70
BstF5I GGATG 3 cut(s) 146, 196, 253
BstFNI CGCG 3 cut(s) 14, 72, 349
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 4 cut(s) 41, 175, 272, 508
BstMBI GATC 4 cut(s) 38, 172, 269, 505
BstMWI GCNNNNNNNGC 5 cut(s) 72, 78, 110, 395, 634
BstNI CCWGG 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 480
BstUI CGCG 3 cut(s) 14, 72, 349
BstV1I GCAGC 3 cut(s) 111, 206, 704
BstX2I RGATCY 2 cut(s) 38, 269
BstYI RGATCY 2 cut(s) 38, 269
BsuRI GGCC 3 cut(s) 19, 250, 386
BtgI CCRYGG 1 cut(s) 70
BtsCI GGATG 3 cut(s) 146, 196, 253
BtsIMutI CAGTG 2 cut(s) 424, 637
Cac8I GCNNGC 2 cut(s) 106, 156
CfoI GCGC 1 cut(s) 351
Cfr13I GGNCC 2 cut(s) 8, 248
Cfr42I CCGCGG 1 cut(s) 73
CseI GACGC 1 cut(s) 715
Csp6I GTAC 2 cut(s) 278, 605
CviAII CATG 3 cut(s) 275, 304, 649
CviQI GTAC 2 cut(s) 278, 605
DdeI CTNAG 6 cut(s) 21, 56, 288, 362, 416, 608
DpnI GATC 4 cut(s) 40, 174, 271, 507
DpnII GATC 4 cut(s) 38, 172, 269, 505
DraIII CACNNNGTG 1 cut(s) 155
EciI GGCGGA 1 cut(s) 602
Eco130I CCWWGG 1 cut(s) 186
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 83
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 8
Eco57I CTGAAG 2 cut(s) 46, 588
EcoO109I RGGNCCY 2 cut(s) 8, 248
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 480
EcoRV GATATC 1 cut(s) 493
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 3 cut(s) 278, 307, 652
FaqI GGGAC 2 cut(s) 21, 393
FatI CATG 3 cut(s) 274, 303, 648
FauI CCCGC 2 cut(s) 19, 113
FbaI TGATCA 1 cut(s) 172
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 5 cut(s) 73, 125, 195, 387, 693
FokI GGATG 3 cut(s) 153, 203, 240
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 5 cut(s) 73, 125, 195, 387, 693
FspBI CTAG 2 cut(s) 161, 266
GlaI GCGC 1 cut(s) 350
GluI GCNGC 5 cut(s) 73, 125, 195, 387, 693
GsaI CCCAGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 3 cut(s) 19, 250, 386
HgaI GACGC 1 cut(s) 715
HhaI GCGC 1 cut(s) 351
Hin1I GRCGYC 2 cut(s) 96, 707
Hin1II CATG 3 cut(s) 278, 307, 652
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 5 cut(s) 239, 319, 341, 553, 671
HphI GGTGA 1 cut(s) 666
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 5 cut(s) 38, 94, 340, 460, 724
Hpy188III TCNNGA 3 cut(s) 161, 287, 328
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 2 cut(s) 216, 563
HpyCH4III ACNGT 1 cut(s) 539
HpyCH4IV ACGT 2 cut(s) 96, 731
HpyCH4V TGCA 6 cut(s) 194, 202, 398, 637, 665, 692
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 78, 110, 395, 634
HpyF3I CTNAG 6 cut(s) 21, 56, 288, 362, 416, 608
HpySE526I ACGT 2 cut(s) 96, 731
Hsp92I GRCGYC 2 cut(s) 96, 707
Hsp92II CATG 3 cut(s) 278, 307, 652
HspAI GCGC 1 cut(s) 349
KflI GGGWCCC 1 cut(s) 8
Ksp22I TGATCA 1 cut(s) 172
KspI CCGCGG 1 cut(s) 73
Kzo9I GATC 4 cut(s) 38, 172, 269, 505
LmnI GCTCC 2 cut(s) 86, 573
Lsp1109I GCAGC 3 cut(s) 111, 206, 704
LweI GCATC 2 cut(s) 181, 360
MaeI CTAG 2 cut(s) 161, 266
MaeII ACGT 2 cut(s) 96, 731
MaeIII GTNAC 1 cut(s) 131
MalI GATC 4 cut(s) 40, 174, 271, 507
MboI GATC 4 cut(s) 38, 172, 269, 505
MboII GAAGA 1 cut(s) 322
MflI RGATCY 2 cut(s) 38, 269
MhlI GDGCHC 2 cut(s) 83, 160
MluCI AATT 3 cut(s) 145, 390, 659
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 1 cut(s) 117
MspA1I CMGCKG 4 cut(s) 72, 197, 566, 695
MspR9I CCNGG 1 cut(s) 482
MvaI CCWGG 1 cut(s) 482
MvnI CGCG 3 cut(s) 14, 72, 349
MwoI GCNNNNNNNGC 5 cut(s) 72, 78, 110, 395, 634
NdeII GATC 4 cut(s) 38, 172, 269, 505
NlaIII CATG 3 cut(s) 278, 307, 652
NlaIV GGNNCC 5 cut(s) 9, 10, 40, 82, 249
PceI AGGCCT 1 cut(s) 19
PfeI GAWTC 4 cut(s) 239, 341, 553, 671
PflMI CCANNNNNTGG 1 cut(s) 487
PkrI GCNGC 5 cut(s) 74, 126, 196, 388, 694
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
PpuMI RGGWCCY 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 5 cut(s) 9, 10, 40, 82, 249
PspPI GGNCC 2 cut(s) 8, 248
PspPPI RGGWCCY 1 cut(s) 8
PsuI RGATCY 2 cut(s) 38, 269
PvuII CAGCTG 3 cut(s) 197, 566, 695
RsaI GTAC 2 cut(s) 279, 606
RsaNI GTAC 2 cut(s) 278, 605
SacII CCGCGG 1 cut(s) 73
SatI GCNGC 5 cut(s) 73, 125, 195, 387, 693
Sau3AI GATC 4 cut(s) 38, 172, 269, 505
Sau96I GGNCC 2 cut(s) 8, 248
ScaI AGTACT 1 cut(s) 606
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 2 cut(s) 83, 160
SfaNI GCATC 2 cut(s) 181, 360
Sfr303I CCGCGG 1 cut(s) 73
SgrBI CCGCGG 1 cut(s) 73
SinI GGWCC 1 cut(s) 8
Sse9I AATT 3 cut(s) 145, 390, 659
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 7 cut(s) 12, 70, 72, 106, 231, 387, 587
SspI AATATT 1 cut(s) 455
SspMI CTAG 2 cut(s) 161, 266
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 480
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 539
TaiI ACGT 2 cut(s) 99, 734
TaqI TCGA 1 cut(s) 242
TasI AATT 3 cut(s) 145, 390, 659
TatI WGTACW 1 cut(s) 604
TauI GCSGC 2 cut(s) 75, 389
TfiI GAWTC 4 cut(s) 239, 341, 553, 671
TscAI CASTG 2 cut(s) 424, 644
TseI GCWGC 3 cut(s) 124, 194, 692
TspDTI ATGAA 1 cut(s) 545
TspRI CASTG 2 cut(s) 424, 644
Van91I CCANNNNNTGG 1 cut(s) 487
VpaK11BI GGWCC 1 cut(s) 8
XapI RAATTY 2 cut(s) 145, 659
XbaI TCTAGA 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 1 cut(s) 636
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 2 cut(s) 161, 266
ZraI GACGTC 1 cut(s) 97
ZrmI AGTACT 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.