FvH4_6g11460

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001330
Physical Location & Seq
Reverse (-)
6880182 .. 6882820
2639 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g11460.t1

Sequence Viewer

Length: 729 bp
ATGGTCGGACCAATAAGACCGCAGTTTGTGCTGTTTGGTTCGTCCATCGTCCAGTTCAGTTTCAGCAATGGAGGTTGGGGTGCCATTCTTGCTGACTTATATTCTCGGAAGGCGGATATAGTTGTGCGTGGCTATGCTGGTTGGAATTCAAGGCAAGGTCTGCAGGTTTTAGAGCAAGTGTTCCCAAAGGATGCAAGTATTCAGCCATCCTTGGTGATAGTCTATTTTGGTGGTAATGATTCAATGCTCCCCGATCCACTAGGCTTAAGCCCTCATGTACCTCTTCCTGAGTATGTAGAGAACATGAGAAAGATTGCTACACATCTGAAGAACCTATCGGAGAAAACCCGCTTTATCTTTCTGACTGCTCCTCCTGTCAACGAGGAGCAAATGCGAAAAGCGTTTAGTGGCATCATGAGGGAGAATAGTCGAACAAACAAGCTGTGCCAAATGTATTCAGATGCTTGTGTAGAGCTTTGCAGAGATATGGATATCAAGGCTGTTGATCTTTTTACTTCAATGCAGAAAAGAAAGGACTGGTCGACTATGTGCTTTACGGATGGTATCCATTTTTCATCGGAGGGGAGCAAGATCGTGGCGGAGGAGATACTGAAGGTCCTTGAAGAAGCAGACTGGGAACCCAGCCTGCAATGGAACTCATTGCCAACCGAATTTGAAGAGTATTCACCTTATGCTCCCACTCGGGTTGATACAAAAAAACAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

243

Amino Acids

27.44

Weight (kDa)

5.51

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 203 2.6e-23 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 197 3.1e-22 GDSL-like Lipase/Acylhydrolase family
DUF459 PF04311 67 - 205 1.1e-06 Protein of unknown function (DUF459)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 154
AccB1I GGYRCC 1 cut(s) 80
AccI GTMKAC 1 cut(s) 542
AciI CCGC 4 cut(s) 20, 113, 349, 599
AclWI GGATC 1 cut(s) 248
AcsI RAATTY 2 cut(s) 145, 671
AcuI CTGAAG 2 cut(s) 347, 632
AfaI GTAC 1 cut(s) 279
AflII CTTAAG 1 cut(s) 265
AgsI TTSAA 5 cut(s) 150, 243, 519, 623, 677
AluBI AGCT 2 cut(s) 442, 475
AluI AGCT 2 cut(s) 442, 475
AlwI GGATC 1 cut(s) 248
Ama87I CYCGRG 1 cut(s) 702
ApoI RAATTY 2 cut(s) 145, 671
AspS9I GGNCC 2 cut(s) 8, 616
AsuHPI GGTGA 2 cut(s) 226, 678
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 2 cut(s) 8, 616
BanI GGYRCC 1 cut(s) 80
BccI CCATC 3 cut(s) 53, 214, 554
BciVI GTATCC 1 cut(s) 575
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 161
BfrI CTTAAG 1 cut(s) 265
BfuAI ACCTGC 1 cut(s) 154
BfuI GTATCC 1 cut(s) 575
Bme18I GGWCC 2 cut(s) 8, 616
BmeT110I CYCGRG 1 cut(s) 702
BmgT120I GGNCC 2 cut(s) 8, 616
BmiI GGNNCC 2 cut(s) 82, 639
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 3 cut(s) 181, 420, 451
BmuI ACTGGG 1 cut(s) 643
BsaJI CCNNGG 1 cut(s) 210
BsaXI ACNNNNNCTCC 2 cut(s) 355, 385
Bse1I ACTGG 3 cut(s) 52, 542, 638
Bse3DI GCAATG 3 cut(s) 73, 656, 659
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 3 cut(s) 196, 206, 565
BseMI GCAATG 3 cut(s) 73, 656, 659
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 3 cut(s) 52, 542, 638
BseRI GAGGAG 3 cut(s) 360, 398, 617
BseYI CCCAGC 1 cut(s) 641
BshNI GGYRCC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 702
BsoBI CYCGRG 1 cut(s) 702
Bsp143I GATC 3 cut(s) 253, 505, 591
BspACI CCGC 4 cut(s) 20, 113, 349, 599
BspCNI CTCAG 1 cut(s) 280
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 2 cut(s) 82, 639
BspMAI CTGCAG 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 154
BspPI GGATC 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 80
BspTI CTTAAG 1 cut(s) 265
BsrDI GCAATG 3 cut(s) 73, 656, 659
BsrI ACTGG 3 cut(s) 52, 542, 638
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 3 cut(s) 253, 505, 591
BssT1I CCWWGG 1 cut(s) 210
Bst6I CTCTTC 2 cut(s) 288, 672
BstAFI CTTAAG 1 cut(s) 265
BstAPI GCANNNNNTGC 2 cut(s) 28, 160
BstC8I GCNNGC 1 cut(s) 647
BstDEI CTNAG 1 cut(s) 288
BstF5I GGATG 3 cut(s) 196, 206, 565
BstKTI GATC 3 cut(s) 256, 508, 594
BstMBI GATC 3 cut(s) 253, 505, 591
BstMWI GCNNNNNNNGC 3 cut(s) 28, 89, 160
BstSFI CTRYAG 1 cut(s) 161
BsuI GTATCC 1 cut(s) 575
BtsCI GGATG 3 cut(s) 196, 206, 565
BveI ACCTGC 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 647
CciI TCATGA 1 cut(s) 414
Cfr13I GGNCC 2 cut(s) 8, 616
Csp6I GTAC 1 cut(s) 278
CspCI CAANNNNNGTGG 2 cut(s) 688, 723
CviAII CATG 3 cut(s) 275, 304, 415
CviJI RGCY 8 cut(s) 132, 205, 264, 270, 442, 475, 500, 645
CviKI_1 RGCY 8 cut(s) 132, 205, 264, 270, 442, 475, 500, 645
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 1 cut(s) 288
DpnI GATC 3 cut(s) 255, 507, 593
DpnII GATC 3 cut(s) 253, 505, 591
Eam1104I CTCTTC 2 cut(s) 288, 672
EarI CTCTTC 2 cut(s) 288, 672
EciI GGCGGA 2 cut(s) 128, 614
Eco130I CCWWGG 1 cut(s) 210
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 2 cut(s) 8, 616
Eco57I CTGAAG 2 cut(s) 347, 632
Eco88I CYCGRG 1 cut(s) 702
EcoO109I RGGNCCY 1 cut(s) 616
EcoRI GAATTC 1 cut(s) 145
EcoRV GATATC 1 cut(s) 493
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 3 cut(s) 278, 307, 418
FatI CATG 3 cut(s) 274, 303, 414
FauI CCCGC 1 cut(s) 356
FblI GTMKAC 1 cut(s) 542
FokI GGATG 3 cut(s) 193, 203, 572
FspBI CTAG 1 cut(s) 260
GsaI CCCAGC 1 cut(s) 645
Hin1II CATG 3 cut(s) 278, 307, 418
HincII GTYRAC 2 cut(s) 379, 543
HindII GTYRAC 2 cut(s) 379, 543
HinfI GANTC 1 cut(s) 239
HphI GGTGA 2 cut(s) 226, 678
Hpy166II GTNNAC 2 cut(s) 379, 543
Hpy188I TCNGA 7 cut(s) 8, 108, 327, 340, 363, 460, 580
Hpy188III TCNNGA 2 cut(s) 287, 415
Hpy8I GTNNAC 2 cut(s) 379, 543
HpyAV CCTTC 2 cut(s) 103, 607
HpyCH4V TGCA 5 cut(s) 163, 194, 480, 523, 649
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 89, 160
HpyF3I CTNAG 1 cut(s) 288
Hsp92II CATG 3 cut(s) 278, 307, 418
Kzo9I GATC 3 cut(s) 253, 505, 591
LmnI GCTCC 5 cut(s) 252, 373, 385, 585, 700
LpnPI CCDG 9 cut(s) 65, 123, 149, 300, 387, 523, 619, 655, 659
LweI GCATC 3 cut(s) 181, 420, 451
MaeI CTAG 1 cut(s) 260
MalI GATC 3 cut(s) 255, 507, 593
MboI GATC 3 cut(s) 253, 505, 591
MboII GAAGA 4 cut(s) 275, 340, 635, 689
MluCI AATT 2 cut(s) 145, 671
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 8 cut(s) 65, 282, 291, 376, 381, 411, 574, 595
MseI TTAA 1 cut(s) 266
MspCI CTTAAG 1 cut(s) 265
MwoI GCNNNNNNNGC 3 cut(s) 28, 89, 160
NdeII GATC 3 cut(s) 253, 505, 591
NlaIII CATG 3 cut(s) 278, 307, 418
NlaIV GGNNCC 2 cut(s) 82, 639
PagI TCATGA 1 cut(s) 414
PfeI GAWTC 1 cut(s) 239
PpuMI RGGWCCY 1 cut(s) 616
Psp5II RGGWCCY 1 cut(s) 616
PspFI CCCAGC 1 cut(s) 641
PspN4I GGNNCC 2 cut(s) 82, 639
PspPI GGNCC 2 cut(s) 8, 616
PspPPI RGGWCCY 1 cut(s) 616
PstI CTGCAG 1 cut(s) 165
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
SalI GTCGAC 1 cut(s) 541
SaqAI TTAA 1 cut(s) 266
Sau3AI GATC 3 cut(s) 253, 505, 591
Sau96I GGNCC 2 cut(s) 8, 616
SetI ASST 9 cut(s) 76, 160, 168, 283, 336, 444, 477, 618, 691
SfaNI GCATC 3 cut(s) 181, 420, 451
SfcI CTRYAG 1 cut(s) 161
SinI GGWCC 2 cut(s) 8, 616
SmlI CTYRAG 1 cut(s) 265
SmoI CTYRAG 1 cut(s) 265
Sse9I AATT 2 cut(s) 145, 671
SsiI CCGC 4 cut(s) 20, 113, 349, 599
SspMI CTAG 1 cut(s) 260
StyI CCWWGG 1 cut(s) 210
TaqI TCGA 2 cut(s) 430, 542
TasI AATT 2 cut(s) 145, 671
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TspDTI ATGAA 1 cut(s) 564
TspGWI ACGGA 1 cut(s) 572
Vha464I CTTAAG 1 cut(s) 265
VpaK11BI GGWCC 2 cut(s) 8, 616
XapI RAATTY 2 cut(s) 145, 671
XmiI GTMKAC 1 cut(s) 542
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.