FvH4_6g11470

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001330
Physical Location & Seq
Reverse (-)
6883001 .. 6885948
2948 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g11470.t1

Sequence Viewer

Length: 783 bp
ATGGTGGGACCTTCAAGACCTCAGTTTGTGCTGTTTGGCTCCTCCATCGTTCAGTACAGTTTCAGCAATGGAGGCTGGGGTGCCATTCTTGCAGACTTGTATTCTCGTAAGGCTGATATACTTGTTCGAGGATACGCTGGTTGGAACTCGAGGCGTGCTGTACAGGTTTTAGAACAAGTTTTTCCAAAGGATGCAACTATTCAGCCATCATTGGTGATGGTCTATTTTGGTGGTAATGATTCCATGCGCCCTAATCCACTAGGCTTAGGCCCTCATGTTCCTCTTCCTGAATATATAGAAAATATGAGAAAGATTGCTACTCATTTACAAAGCCTTTCGGAGAAAACTCGCATCATCTTTCTCTCTGCTCCTCCTGTCAACGAGGAACATATTCGTGAGCAGTTGAGTGGCATAATTGGGGAGCTGAATCGAACAAACAAGCTATGCAAAATATATTCAGATGCTTGTTTAGAGCTTTGCAGGGAGATGGATATCAAGGCTGTTGATCTCTGTACTTCAATTCAGAAGAGAAAGGACTGGGAAACTACTTGCTTTACTGATGGAATCCATCTTTCATCTGAGGGAAGCAAGATTGTGGCGGAGGAGATACTGAAAGTTCTAAAAGAGGCGGACTGGGAACCAAGCCTGCAGTGGAACTCATTGGCAACAGAGTTCGGAGAGGATTCCCCTTATGATCCCGTCGGTACTGATGGAAAGACTATGAATGTCTCTGATGATGGCGTTGCTAGACTCATGCAGTGGGGAATAAGCAATAGGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

261

Amino Acids

29.04

Weight (kDa)

5.34

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 203 4.2e-21 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 197 5.4e-20 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 80
AciI CCGC 2 cut(s) 599, 629
AclWI GGATC 1 cut(s) 689
AfaI GTAC 4 cut(s) 56, 162, 514, 706
AgsI TTSAA 2 cut(s) 15, 519
AluBI AGCT 3 cut(s) 424, 442, 475
AluI AGCT 3 cut(s) 424, 442, 475
Alw26I GTCTC 1 cut(s) 733
AlwI GGATC 1 cut(s) 689
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 1 cut(s) 268
Asp700I GAANNNNTTC 1 cut(s) 390
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 2 cut(s) 8, 269
AsuHPI GGTGA 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 8
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BanI GGYRCC 1 cut(s) 80
BccI CCATC 8 cut(s) 53, 211, 214, 481, 554, 576, 704, 731
BciVI GTATCC 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 733
BfaI CTAG 2 cut(s) 260, 747
BfmI CTRYAG 1 cut(s) 647
BfuI GTATCC 1 cut(s) 125
Bme18I GGWCC 1 cut(s) 8
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 8, 269
BmiI GGNNCC 4 cut(s) 9, 40, 82, 639
BmrI ACTGGG 2 cut(s) 547, 643
BmsI GCATC 3 cut(s) 181, 360, 451
BmuI ACTGGG 2 cut(s) 547, 643
Bpu10I CCTNAGC 1 cut(s) 265
BsaBI GATNNNNATC 1 cut(s) 491
Bse1I ACTGG 2 cut(s) 542, 638
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 491
BseGI GGATG 1 cut(s) 196
BseJI GATNNNNATC 1 cut(s) 491
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 2 cut(s) 35, 570
BseNI ACTGG 2 cut(s) 542, 638
BseRI GAGGAG 3 cut(s) 31, 360, 617
BseYI CCCAGC 1 cut(s) 75
BshFI GGCC 1 cut(s) 270
BshNI GGYRCC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 148
BslFI GGGAC 1 cut(s) 21
BsmAI GTCTC 1 cut(s) 733
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 1 cut(s) 270
BsoBI CYCGRG 1 cut(s) 148
Bsp1407I TGTACA 1 cut(s) 160
Bsp143I GATC 2 cut(s) 505, 694
BspACI CCGC 2 cut(s) 599, 629
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 2 cut(s) 34, 571
BspLI GGNNCC 4 cut(s) 9, 40, 82, 639
BspMAI CTGCAG 1 cut(s) 651
BspPI GGATC 1 cut(s) 689
BspT107I GGYRCC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 73
BsrGI TGTACA 1 cut(s) 160
BsrI ACTGG 2 cut(s) 542, 638
BssMI GATC 2 cut(s) 505, 694
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 2 cut(s) 288, 521
BstAUI TGTACA 1 cut(s) 160
BstC8I GCNNGC 2 cut(s) 156, 647
BstDEI CTNAG 3 cut(s) 21, 265, 579
BstF5I GGATG 1 cut(s) 196
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 2 cut(s) 508, 697
BstMAI GTCTC 1 cut(s) 733
BstMBI GATC 2 cut(s) 505, 694
BstMWI GCNNNNNNNGC 2 cut(s) 72, 89
BstSFI CTRYAG 1 cut(s) 647
BsuI GTATCC 1 cut(s) 125
BsuRI GGCC 1 cut(s) 270
BtsCI GGATG 1 cut(s) 196
BtsI GCAGTG 2 cut(s) 656, 764
BtsIMutI CAGTG 2 cut(s) 656, 764
Cac8I GCNNGC 2 cut(s) 156, 647
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 2 cut(s) 8, 269
Csp6I GTAC 4 cut(s) 55, 161, 513, 705
CviAII CATG 3 cut(s) 244, 275, 754
CviQI GTAC 4 cut(s) 55, 161, 513, 705
DdeI CTNAG 3 cut(s) 21, 265, 579
DpnI GATC 2 cut(s) 507, 696
DpnII GATC 2 cut(s) 505, 694
Eam1104I CTCTTC 2 cut(s) 288, 521
EarI CTCTTC 2 cut(s) 288, 521
EciI GGCGGA 2 cut(s) 614, 644
Eco32I GATATC 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 8
Eco88I CYCGRG 1 cut(s) 148
EcoO109I RGGNCCY 2 cut(s) 8, 269
EcoRV GATATC 1 cut(s) 493
FaeI CATG 3 cut(s) 247, 278, 757
FaqI GGGAC 1 cut(s) 21
FatI CATG 3 cut(s) 243, 274, 753
FokI GGATG 1 cut(s) 203
FspBI CTAG 2 cut(s) 260, 747
GlaI GCGC 1 cut(s) 248
GsaI CCCAGC 1 cut(s) 79
HaeIII GGCC 1 cut(s) 270
HhaI GCGC 1 cut(s) 249
Hin1II CATG 3 cut(s) 247, 278, 757
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 5 cut(s) 239, 427, 564, 683, 750
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 6 cut(s) 340, 460, 525, 580, 677, 733
Hpy188III TCNNGA 3 cut(s) 15, 287, 395
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 1 cut(s) 704
HpyAV CCTTC 1 cut(s) 21
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4V TGCA 6 cut(s) 92, 194, 447, 480, 649, 757
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 89
HpyF3I CTNAG 3 cut(s) 21, 265, 579
Hsp92II CATG 3 cut(s) 247, 278, 757
HspAI GCGC 1 cut(s) 247
Kzo9I GATC 2 cut(s) 505, 694
LmnI GCTCC 3 cut(s) 44, 373, 421
LpnPI CCDG 9 cut(s) 61, 123, 149, 300, 387, 466, 523, 619, 659
LweI GCATC 3 cut(s) 181, 360, 451
MaeI CTAG 2 cut(s) 260, 747
MalI GATC 2 cut(s) 507, 696
MboI GATC 2 cut(s) 505, 694
MboII GAAGA 2 cut(s) 275, 538
MluCI AATT 3 cut(s) 414, 519, 778
MlyI GAGTC 1 cut(s) 744
MmeI TCCRAC 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 390
MseI TTAA 1 cut(s) 781
MslI CAYNNNNRTG 1 cut(s) 393
MwoI GCNNNNNNNGC 2 cut(s) 72, 89
NdeII GATC 2 cut(s) 505, 694
NlaIII CATG 3 cut(s) 247, 278, 757
NlaIV GGNNCC 4 cut(s) 9, 40, 82, 639
PaeR7I CTCGAG 1 cut(s) 148
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 4 cut(s) 239, 427, 564, 683
PleI GAGTC 1 cut(s) 744
PpsI GAGTC 1 cut(s) 744
PpuMI RGGWCCY 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 8
PspFI CCCAGC 1 cut(s) 75
PspN4I GGNNCC 4 cut(s) 9, 40, 82, 639
PspPI GGNCC 2 cut(s) 8, 269
PspPPI RGGWCCY 1 cut(s) 8
PspXI VCTCGAGB 1 cut(s) 148
PstI CTGCAG 1 cut(s) 651
RsaI GTAC 4 cut(s) 56, 162, 514, 706
RsaNI GTAC 4 cut(s) 55, 161, 513, 705
RseI CAYNNNNRTG 1 cut(s) 393
SaqAI TTAA 1 cut(s) 781
Sau3AI GATC 2 cut(s) 505, 694
Sau96I GGNCC 2 cut(s) 8, 269
SchI GAGTC 1 cut(s) 744
SetI ASST 6 cut(s) 13, 22, 168, 426, 444, 477
SfaNI GCATC 3 cut(s) 181, 360, 451
SfcI CTRYAG 1 cut(s) 647
Sfr274I CTCGAG 1 cut(s) 148
SinI GGWCC 1 cut(s) 8
SlaI CTCGAG 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 393
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 3 cut(s) 414, 519, 778
SsiI CCGC 2 cut(s) 599, 629
SspMI CTAG 2 cut(s) 260, 747
TaaI ACNGT 1 cut(s) 59
TaqI TCGA 3 cut(s) 127, 149, 430
TasI AATT 3 cut(s) 414, 519, 778
TatI WGTACW 3 cut(s) 54, 160, 512
TfiI GAWTC 4 cut(s) 239, 427, 564, 683
Tru1I TTAA 1 cut(s) 781
Tru9I TTAA 1 cut(s) 781
TscAI CASTG 2 cut(s) 656, 764
TspDTI ATGAA 2 cut(s) 564, 737
TspRI CASTG 2 cut(s) 656, 764
VpaK11BI GGWCC 1 cut(s) 8
XcmI CCANNNNNNNNNTGG 1 cut(s) 648
XhoI CTCGAG 1 cut(s) 148
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 2 cut(s) 260, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.