RchiOBHm_Chr3g0463161

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001330
Physical Location & Seq
Reverse (-)
10636344 .. 10639518
3175 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42948

Sequence Viewer

Length: 789 bp
ATGGTGGGACCTTCAAGACCTCAGTTTGTGCTGTTTGGCTCCTCCATCGTTCAGTACAGTTTCAGCAATGGAGGCTGGGGTGCCATTCTTGCAGACTTATATTCTCGTAAGGCTGATATACTTCTTCGAGGATACGCTGGTTGGAACTCGAGGCGTGCTGTAAAGGTTTTAGAGCAGGTTTTTCCAAAGGATGCAACTATTCAGCCATCATTGGTGATAGTCTATTTCGGTGGTAATGATTCAATGCGCCCTAATCCACTAGGCCTAGGCCCTCATGTACCTCTTTCTGAATATGTGGAAAATATGAGGAAGATTGCTACTCATTTACAGAGCCTTTCAGAGAAAACTCGCATTATCTTTCTCTCTGCTCCTCCTGTCAACGAGGAACAAATTCGTGAGCATTTAAGAACCAGTGGCATAATTGGGGAGCTGAATCGAACAAACAAGCTATGCAAAATATATTCAGATGCTTGTTTAGAGCTGTGCAGAGAGATGGATATCAAGGCTGTTGATCTCTTCACTTCAATTCAGAAGAGAAAAAACTGGGAAACTACTTGCTTTACTGATGGAGTCCATTTTTCATCTGAGGGGAGCAAGATTGTGGCGGAGGAGATATTACAGGTCCTTAAAGAGGCGAACTGGGAAAGCAGCCTGCATTGGACGTCATTGGCAACGGAATTCGGAGAGGATTCCCCTTATGATCCCGTCGGTATTGATGGAAAAACTATGAATGTCTCAGAGGATGGCGTTGCGAGACTTCTGCAGTGGGGAATAAGCAACGGGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

262

Amino Acids

29.27

Weight (kDa)

5.8

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 205 5e-22 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 11 - 199 1e-21 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 166
AccB1I GGYRCC 1 cut(s) 80
AciI CCGC 1 cut(s) 605
AclWI GGATC 1 cut(s) 695
AcsI RAATTY 2 cut(s) 390, 677
AcyI GRCGYC 1 cut(s) 662
AfaI GTAC 2 cut(s) 56, 279
AfiI CCNNNNNNNGG 1 cut(s) 631
AgsI TTSAA 3 cut(s) 15, 243, 525
AluBI AGCT 3 cut(s) 430, 448, 481
AluI AGCT 3 cut(s) 430, 448, 481
Alw26I GTCTC 2 cut(s) 739, 748
AlwI GGATC 1 cut(s) 695
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 2 cut(s) 262, 268
ApeKI GCWGC 1 cut(s) 648
ApoI RAATTY 2 cut(s) 390, 677
Asp700I GAANNNNTTC 1 cut(s) 390
AspA2I CCTAGG 1 cut(s) 265
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 3 cut(s) 8, 269, 622
AsuHPI GGTGA 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 2 cut(s) 8, 622
AvrII CCTAGG 1 cut(s) 265
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BanI GGYRCC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 660
BccI CCATC 6 cut(s) 53, 214, 487, 560, 710, 737
BcgI CGANNNNNNTGC 2 cut(s) 742, 776
BciVI GTATCC 1 cut(s) 125
BcoDI GTCTC 2 cut(s) 739, 748
BfaI CTAG 2 cut(s) 260, 266
BfmI CTRYAG 1 cut(s) 761
BfuAI ACCTGC 1 cut(s) 166
BfuI GTATCC 1 cut(s) 125
BisI GCNGC 1 cut(s) 649
BlnI CCTAGG 1 cut(s) 265
BlsI GCNGC 1 cut(s) 650
Bme18I GGWCC 2 cut(s) 8, 622
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 3 cut(s) 8, 269, 622
BmiI GGNNCC 3 cut(s) 9, 40, 82
BmrI ACTGGG 2 cut(s) 553, 649
BmsI GCATC 2 cut(s) 181, 457
BmuI ACTGGG 2 cut(s) 553, 649
BsaBI GATNNNNATC 1 cut(s) 497
BsaHI GRCGYC 1 cut(s) 662
BsaJI CCNNGG 1 cut(s) 265
Bsc4I CCNNNNNNNGG 1 cut(s) 631
Bse1I ACTGG 3 cut(s) 411, 548, 644
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 497
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 2 cut(s) 196, 748
BseJI GATNNNNATC 1 cut(s) 497
BseLI CCNNNNNNNGG 1 cut(s) 631
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 3 cut(s) 35, 576, 750
BseNI ACTGG 3 cut(s) 411, 548, 644
BseRI GAGGAG 3 cut(s) 31, 360, 623
BseXI GCAGC 1 cut(s) 660
BseYI CCCAGC 1 cut(s) 75
BsgI GTGCAG 1 cut(s) 505
BshFI GGCC 2 cut(s) 264, 270
BshNI GGYRCC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 148
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 1 cut(s) 631
BsmAI GTCTC 2 cut(s) 739, 748
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 2 cut(s) 264, 270
BsoBI CYCGRG 1 cut(s) 148
Bsp143I GATC 2 cut(s) 511, 700
BspACI CCGC 1 cut(s) 605
BspANI GGCC 2 cut(s) 264, 270
BspCNI CTCAG 3 cut(s) 34, 577, 749
BspLI GGNNCC 3 cut(s) 9, 40, 82
BspMAI CTGCAG 1 cut(s) 765
BspMI ACCTGC 1 cut(s) 166
BspPI GGATC 1 cut(s) 695
BspT107I GGYRCC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 73
BsrI ACTGG 3 cut(s) 411, 548, 644
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 2 cut(s) 511, 700
BssNI GRCGYC 1 cut(s) 662
BssT1I CCWWGG 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 2 cut(s) 521, 527
BstACI GRCGYC 1 cut(s) 662
BstC8I GCNNGC 2 cut(s) 156, 653
BstDEI CTNAG 3 cut(s) 21, 585, 736
BstENI CCTNNNNNAGG 1 cut(s) 629
BstF5I GGATG 2 cut(s) 196, 748
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 2 cut(s) 514, 703
BstMAI GTCTC 2 cut(s) 739, 748
BstMBI GATC 2 cut(s) 511, 700
BstMWI GCNNNNNNNGC 2 cut(s) 72, 89
BstSFI CTRYAG 1 cut(s) 761
BstV1I GCAGC 1 cut(s) 660
BsuI GTATCC 1 cut(s) 125
BsuRI GGCC 2 cut(s) 264, 270
BtsCI GGATG 2 cut(s) 196, 748
BtsI GCAGTG 1 cut(s) 770
BtsIMutI CAGTG 2 cut(s) 418, 770
BveI ACCTGC 1 cut(s) 166
Cac8I GCNNGC 2 cut(s) 156, 653
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 3 cut(s) 8, 269, 622
Csp6I GTAC 2 cut(s) 55, 278
CviAII CATG 1 cut(s) 275
CviQI GTAC 2 cut(s) 55, 278
DdeI CTNAG 3 cut(s) 21, 585, 736
DpnI GATC 2 cut(s) 513, 702
DpnII GATC 2 cut(s) 511, 700
Eam1104I CTCTTC 2 cut(s) 521, 527
EarI CTCTTC 2 cut(s) 521, 527
EciI GGCGGA 1 cut(s) 620
Eco130I CCWWGG 1 cut(s) 265
Eco147I AGGCCT 1 cut(s) 264
Eco32I GATATC 1 cut(s) 499
Eco47I GGWCC 2 cut(s) 8, 622
Eco88I CYCGRG 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 629
EcoO109I RGGNCCY 3 cut(s) 8, 269, 622
EcoRI GAATTC 1 cut(s) 677
EcoRV GATATC 1 cut(s) 499
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 1 cut(s) 278
FaqI GGGAC 1 cut(s) 21
FatI CATG 1 cut(s) 274
Fnu4HI GCNGC 1 cut(s) 649
FokI GGATG 2 cut(s) 203, 755
Fsp4HI GCNGC 1 cut(s) 649
FspBI CTAG 2 cut(s) 260, 266
GlaI GCGC 1 cut(s) 248
GluI GCNGC 1 cut(s) 649
GsaI CCCAGC 1 cut(s) 79
HaeIII GGCC 2 cut(s) 264, 270
HhaI GCGC 1 cut(s) 249
Hin1I GRCGYC 1 cut(s) 662
Hin1II CATG 1 cut(s) 278
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 4 cut(s) 239, 433, 570, 689
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 7 cut(s) 289, 340, 466, 531, 586, 683, 739
Hpy188III TCNNGA 2 cut(s) 15, 395
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 1 cut(s) 710
HpyAV CCTTC 1 cut(s) 21
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 1 cut(s) 662
HpyCH4V TGCA 6 cut(s) 92, 194, 453, 486, 655, 763
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 89
HpyF3I CTNAG 3 cut(s) 21, 585, 736
HpySE526I ACGT 1 cut(s) 662
Hsp92I GRCGYC 1 cut(s) 662
Hsp92II CATG 1 cut(s) 278
HspAI GCGC 1 cut(s) 247
Kzo9I GATC 2 cut(s) 511, 700
LmnI GCTCC 4 cut(s) 44, 373, 427, 591
LpnPI CCDG 9 cut(s) 61, 123, 161, 387, 424, 529, 605, 625, 665
Lsp1109I GCAGC 1 cut(s) 660
LweI GCATC 2 cut(s) 181, 457
MaeI CTAG 2 cut(s) 260, 266
MaeII ACGT 1 cut(s) 662
MalI GATC 2 cut(s) 513, 702
MboI GATC 2 cut(s) 511, 700
MboII GAAGA 4 cut(s) 116, 322, 508, 544
MluCI AATT 4 cut(s) 390, 420, 525, 677
MlyI GAGTC 1 cut(s) 579
MmeI TCCRAC 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 390
MseI TTAA 3 cut(s) 404, 627, 787
MwoI GCNNNNNNNGC 2 cut(s) 72, 89
NdeII GATC 2 cut(s) 511, 700
NlaIII CATG 1 cut(s) 278
NlaIV GGNNCC 3 cut(s) 9, 40, 82
PaeR7I CTCGAG 1 cut(s) 148
PceI AGGCCT 1 cut(s) 264
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 3 cut(s) 239, 433, 689
PkrI GCNGC 1 cut(s) 650
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
PpuMI RGGWCCY 2 cut(s) 8, 622
Psp5II RGGWCCY 2 cut(s) 8, 622
PspFI CCCAGC 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 9, 40, 82
PspPI GGNCC 3 cut(s) 8, 269, 622
PspPPI RGGWCCY 2 cut(s) 8, 622
PspXI VCTCGAGB 1 cut(s) 148
PstI CTGCAG 1 cut(s) 765
RsaI GTAC 2 cut(s) 56, 279
RsaNI GTAC 2 cut(s) 55, 278
SaqAI TTAA 3 cut(s) 404, 627, 787
SatI GCNGC 1 cut(s) 649
Sau3AI GATC 2 cut(s) 511, 700
Sau96I GGNCC 3 cut(s) 8, 269, 622
SchI GAGTC 1 cut(s) 579
SfaNI GCATC 2 cut(s) 181, 457
SfcI CTRYAG 1 cut(s) 761
Sfr274I CTCGAG 1 cut(s) 148
SinI GGWCC 2 cut(s) 8, 622
SlaI CTCGAG 1 cut(s) 148
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 4 cut(s) 390, 420, 525, 677
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 1 cut(s) 605
SspMI CTAG 2 cut(s) 260, 266
StuI AGGCCT 1 cut(s) 264
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 1 cut(s) 665
TaqI TCGA 3 cut(s) 127, 149, 436
TasI AATT 4 cut(s) 390, 420, 525, 677
TatI WGTACW 1 cut(s) 54
TfiI GAWTC 3 cut(s) 239, 433, 689
Tru1I TTAA 3 cut(s) 404, 627, 787
Tru9I TTAA 3 cut(s) 404, 627, 787
TscAI CASTG 2 cut(s) 418, 770
TseI GCWGC 1 cut(s) 648
TspDTI ATGAA 2 cut(s) 570, 743
TspGWI ACGGA 1 cut(s) 689
TspRI CASTG 2 cut(s) 418, 770
VpaK11BI GGWCC 2 cut(s) 8, 622
XagI CCTNNNNNAGG 1 cut(s) 629
XapI RAATTY 2 cut(s) 390, 677
XhoI CTCGAG 1 cut(s) 148
XmaJI CCTAGG 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 2 cut(s) 260, 266
ZraI GACGTC 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.