GDSL esterase lipase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 559 |
| AccII | CGCG | 1 cut(s) | 910 |
| AciI | CCGC | 4 cut(s) | 206, 392, 573, 613 |
| AclWI | GGATC | 3 cut(s) | 47, 452, 462 |
| AcoI | YGGCCR | 2 cut(s) | 3, 60 |
| AcsI | RAATTY | 2 cut(s) | 503, 842 |
| AcuI | CTGAAG | 1 cut(s) | 587 |
| AfaI | GTAC | 1 cut(s) | 158 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 559 |
| AgsI | TTSAA | 5 cut(s) | 218, 260, 542, 803, 966 |
| AjnI | CCWGG | 2 cut(s) | 676, 747 |
| AluBI | AGCT | 5 cut(s) | 58, 308, 433, 592, 796 |
| AluI | AGCT | 5 cut(s) | 58, 308, 433, 592, 796 |
| Alw21I | GWGCWC | 1 cut(s) | 723 |
| Alw44I | GTGCAC | 1 cut(s) | 719 |
| AlwI | GGATC | 3 cut(s) | 47, 452, 462 |
| AoxI | GGCC | 3 cut(s) | 3, 60, 687 |
| ApaLI | GTGCAC | 1 cut(s) | 719 |
| ApeKI | GCWGC | 3 cut(s) | 77, 695, 700 |
| ApoI | RAATTY | 2 cut(s) | 503, 842 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 935, 967 |
| AspLEI | GCGC | 1 cut(s) | 700 |
| AsuHPI | GGTGA | 1 cut(s) | 17 |
| BaeGI | GKGCMC | 1 cut(s) | 723 |
| Bbv12I | GWGCWC | 1 cut(s) | 723 |
| BbvI | GCAGC | 3 cut(s) | 64, 682, 687 |
| BccI | CCATC | 2 cut(s) | 333, 622 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 857, 891 |
| BciT130I | CCWGG | 2 cut(s) | 678, 749 |
| BfaI | CTAG | 2 cut(s) | 125, 797 |
| BisI | GCNGC | 4 cut(s) | 78, 393, 696, 701 |
| BlsI | GCNGC | 4 cut(s) | 79, 394, 697, 702 |
| Bme1390I | CCNGG | 2 cut(s) | 678, 749 |
| BmrFI | CCNGG | 2 cut(s) | 678, 749 |
| BmsI | GCATC | 1 cut(s) | 646 |
| BpmI | CTGGAG | 1 cut(s) | 770 |
| BsaJI | CCNNGG | 2 cut(s) | 677, 690 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 559 |
| BseBI | CCWGG | 2 cut(s) | 678, 749 |
| BseDI | CCNNGG | 2 cut(s) | 677, 690 |
| BseGI | GGATG | 2 cut(s) | 589, 637 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 559 |
| BseMII | CTCAG | 1 cut(s) | 549 |
| BseRI | GAGGAG | 2 cut(s) | 236, 443 |
| BseSI | GKGCMC | 1 cut(s) | 723 |
| BseXI | GCAGC | 3 cut(s) | 64, 682, 687 |
| BsgI | GTGCAG | 2 cut(s) | 762, 851 |
| Bsh1236I | CGCG | 1 cut(s) | 910 |
| BshFI | GGCC | 3 cut(s) | 5, 62, 689 |
| BsiHKAI | GWGCWC | 1 cut(s) | 723 |
| BslFI | GGGAC | 1 cut(s) | 629 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 559 |
| BsmFI | GGGAC | 1 cut(s) | 629 |
| BsmI | GAATGC | 2 cut(s) | 184, 190 |
| BsnI | GGCC | 3 cut(s) | 5, 62, 689 |
| Bsp1286I | GDGCHC | 1 cut(s) | 723 |
| Bsp143I | GATC | 4 cut(s) | 39, 444, 454, 508 |
| BspACI | CCGC | 4 cut(s) | 206, 392, 573, 613 |
| BspANI | GGCC | 3 cut(s) | 5, 62, 689 |
| BspCNI | CTCAG | 1 cut(s) | 548 |
| BspFNI | CGCG | 1 cut(s) | 910 |
| BspPI | GGATC | 3 cut(s) | 47, 452, 462 |
| BssECI | CCNNGG | 2 cut(s) | 677, 690 |
| BssMI | GATC | 4 cut(s) | 39, 444, 454, 508 |
| BssT1I | CCWWGG | 1 cut(s) | 690 |
| Bst2UI | CCWGG | 2 cut(s) | 678, 749 |
| Bst4CI | ACNGT | 1 cut(s) | 528 |
| Bst6I | CTCTTC | 1 cut(s) | 213 |
| BstC8I | GCNNGC | 2 cut(s) | 60, 758 |
| BstDEI | CTNAG | 2 cut(s) | 335, 535 |
| BstEII | GGTNACC | 1 cut(s) | 208 |
| BstF5I | GGATG | 2 cut(s) | 589, 637 |
| BstFNI | CGCG | 1 cut(s) | 910 |
| BstHHI | GCGC | 1 cut(s) | 700 |
| BstKTI | GATC | 4 cut(s) | 42, 447, 457, 511 |
| BstMBI | GATC | 4 cut(s) | 39, 444, 454, 508 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 55, 598, 695 |
| BstNI | CCWGG | 2 cut(s) | 678, 749 |
| BstNSI | RCATGY | 1 cut(s) | 760 |
| BstPI | GGTNACC | 1 cut(s) | 208 |
| BstSCI | CCNGG | 2 cut(s) | 676, 747 |
| BstSLI | GKGCMC | 1 cut(s) | 723 |
| BstUI | CGCG | 1 cut(s) | 910 |
| BstV1I | GCAGC | 3 cut(s) | 64, 682, 687 |
| BstX2I | RGATCY | 2 cut(s) | 39, 444 |
| BstYI | RGATCY | 2 cut(s) | 39, 444 |
| BsuRI | GGCC | 3 cut(s) | 5, 62, 689 |
| BtsCI | GGATG | 2 cut(s) | 589, 637 |
| BtsI | GCAGTG | 1 cut(s) | 618 |
| BtsIMutI | CAGTG | 1 cut(s) | 618 |
| Cac8I | GCNNGC | 2 cut(s) | 60, 758 |
| CfoI | GCGC | 1 cut(s) | 700 |
| Csp6I | GTAC | 1 cut(s) | 157 |
| CviAII | CATG | 5 cut(s) | 356, 416, 554, 646, 757 |
| CviQI | GTAC | 1 cut(s) | 157 |
| DdeI | CTNAG | 2 cut(s) | 335, 535 |
| DpnI | GATC | 4 cut(s) | 41, 446, 456, 510 |
| DpnII | GATC | 4 cut(s) | 39, 444, 454, 508 |
| DraI | TTTAAA | 1 cut(s) | 322 |
| EaeI | YGGCCR | 2 cut(s) | 3, 60 |
| Eam1104I | CTCTTC | 1 cut(s) | 213 |
| EarI | CTCTTC | 1 cut(s) | 213 |
| Eco130I | CCWWGG | 1 cut(s) | 690 |
| Eco57I | CTGAAG | 1 cut(s) | 587 |
| Eco91I | GGTNACC | 1 cut(s) | 208 |
| EcoO65I | GGTNACC | 1 cut(s) | 208 |
| EcoRII | CCWGG | 2 cut(s) | 676, 747 |
| EcoT14I | CCWWGG | 1 cut(s) | 690 |
| ErhI | CCWWGG | 1 cut(s) | 690 |
| FaeI | CATG | 5 cut(s) | 359, 419, 557, 649, 760 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 26, 58 |
| FaqI | GGGAC | 1 cut(s) | 629 |
| FatI | CATG | 5 cut(s) | 355, 415, 553, 645, 756 |
| FauI | CCCGC | 1 cut(s) | 606 |
| Fnu4HI | GCNGC | 4 cut(s) | 78, 393, 696, 701 |
| FokI | GGATG | 2 cut(s) | 596, 644 |
| Fsp4HI | GCNGC | 4 cut(s) | 78, 393, 696, 701 |
| FspBI | CTAG | 2 cut(s) | 125, 797 |
| GlaI | GCGC | 1 cut(s) | 699 |
| GluI | GCNGC | 4 cut(s) | 78, 393, 696, 701 |
| GsuI | CTGGAG | 1 cut(s) | 770 |
| HaeIII | GGCC | 3 cut(s) | 5, 62, 689 |
| HhaI | GCGC | 1 cut(s) | 700 |
| Hin1II | CATG | 5 cut(s) | 359, 419, 557, 649, 760 |
| Hin6I | GCGC | 1 cut(s) | 698 |
| HinP1I | GCGC | 1 cut(s) | 698 |
| HincII | GTYRAC | 1 cut(s) | 733 |
| HindII | GTYRAC | 1 cut(s) | 733 |
| HinfI | GANTC | 5 cut(s) | 22, 82, 566, 803, 821 |
| HphI | GGTGA | 1 cut(s) | 17 |
| Hpy166II | GTNNAC | 2 cut(s) | 721, 733 |
| Hpy188I | TCNGA | 5 cut(s) | 495, 606, 793, 853, 879 |
| Hpy188III | TCNNGA | 2 cut(s) | 13, 260 |
| Hpy8I | GTNNAC | 2 cut(s) | 721, 733 |
| HpyAV | CCTTC | 1 cut(s) | 858 |
| HpyCH4III | ACNGT | 1 cut(s) | 528 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 55, 598, 695 |
| HpyF3I | CTNAG | 2 cut(s) | 335, 535 |
| Hsp92II | CATG | 5 cut(s) | 359, 419, 557, 649, 760 |
| HspAI | GCGC | 1 cut(s) | 698 |
| Kzo9I | GATC | 4 cut(s) | 39, 444, 454, 508 |
| LmnI | GCTCC | 2 cut(s) | 223, 430 |
| Lsp1109I | GCAGC | 3 cut(s) | 64, 682, 687 |
| LweI | GCATC | 1 cut(s) | 646 |
| MaeI | CTAG | 2 cut(s) | 125, 797 |
| MaeIII | GTNAC | 4 cut(s) | 208, 269, 911, 982 |
| MalI | GATC | 4 cut(s) | 41, 446, 456, 510 |
| MboI | GATC | 4 cut(s) | 39, 444, 454, 508 |
| MboII | GAAGA | 3 cut(s) | 34, 230, 419 |
| MfeI | CAATTG | 2 cut(s) | 227, 816 |
| MflI | RGATCY | 2 cut(s) | 39, 444 |
| MhlI | GDGCHC | 1 cut(s) | 723 |
| MluCI | AATT | 5 cut(s) | 165, 227, 503, 816, 842 |
| MlyI | GAGTC | 1 cut(s) | 16 |
| MnlI | CCTC | 8 cut(s) | 28, 214, 297, 421, 556, 574, 684, 951 |
| MseI | TTAA | 3 cut(s) | 321, 377, 810 |
| MspA1I | CMGCKG | 1 cut(s) | 58 |
| MspR9I | CCNGG | 2 cut(s) | 678, 749 |
| MunI | CAATTG | 2 cut(s) | 227, 816 |
| Mva1269I | GAATGC | 2 cut(s) | 184, 190 |
| MvaI | CCWGG | 2 cut(s) | 678, 749 |
| MvnI | CGCG | 1 cut(s) | 910 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 55, 598, 695 |
| NdeII | GATC | 4 cut(s) | 39, 444, 454, 508 |
| NlaIII | CATG | 5 cut(s) | 359, 419, 557, 649, 760 |
| NmuCI | GTSAC | 1 cut(s) | 911 |
| NspI | RCATGY | 1 cut(s) | 760 |
| PaeI | GCATGC | 1 cut(s) | 760 |
| PctI | GAATGC | 2 cut(s) | 184, 190 |
| PfeI | GAWTC | 4 cut(s) | 82, 566, 803, 821 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 559 |
| PkrI | GCNGC | 4 cut(s) | 79, 394, 697, 702 |
| PleI | GAGTC | 1 cut(s) | 16 |
| PpsI | GAGTC | 1 cut(s) | 16 |
| Psp6I | CCWGG | 2 cut(s) | 676, 747 |
| PspEI | GGTNACC | 1 cut(s) | 208 |
| PspGI | CCWGG | 2 cut(s) | 676, 747 |
| PsuI | RGATCY | 2 cut(s) | 39, 444 |
| PvuII | CAGCTG | 1 cut(s) | 58 |
| RsaI | GTAC | 1 cut(s) | 158 |
| RsaNI | GTAC | 1 cut(s) | 157 |
| SaqAI | TTAA | 3 cut(s) | 321, 377, 810 |
| SatI | GCNGC | 4 cut(s) | 78, 393, 696, 701 |
| Sau3AI | GATC | 4 cut(s) | 39, 444, 454, 508 |
| SchI | GAGTC | 1 cut(s) | 16 |
| ScrFI | CCNGG | 2 cut(s) | 678, 749 |
| SduI | GDGCHC | 1 cut(s) | 723 |
| SfaNI | GCATC | 1 cut(s) | 646 |
| SphI | GCATGC | 1 cut(s) | 760 |
| Sse9I | AATT | 5 cut(s) | 165, 227, 503, 816, 842 |
| SsiI | CCGC | 4 cut(s) | 206, 392, 573, 613 |
| SspI | AATATT | 1 cut(s) | 375 |
| SspMI | CTAG | 2 cut(s) | 125, 797 |
| StyD4I | CCNGG | 2 cut(s) | 676, 747 |
| StyI | CCWWGG | 1 cut(s) | 690 |
| TaaI | ACNGT | 1 cut(s) | 528 |
| TaqI | TCGA | 2 cut(s) | 20, 941 |
| TasI | AATT | 5 cut(s) | 165, 227, 503, 816, 842 |
| TauI | GCSGC | 1 cut(s) | 395 |
| TfiI | GAWTC | 4 cut(s) | 82, 566, 803, 821 |
| Tru1I | TTAA | 3 cut(s) | 321, 377, 810 |
| Tru9I | TTAA | 3 cut(s) | 321, 377, 810 |
| TscAI | CASTG | 1 cut(s) | 625 |
| TseFI | GTSAC | 1 cut(s) | 911 |
| TseI | GCWGC | 3 cut(s) | 77, 695, 700 |
| Tsp45I | GTSAC | 1 cut(s) | 911 |
| TspDTI | ATGAA | 4 cut(s) | 261, 269, 372, 984 |
| TspRI | CASTG | 1 cut(s) | 625 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 559 |
| VneI | GTGCAC | 1 cut(s) | 719 |
| XapI | RAATTY | 2 cut(s) | 503, 842 |
| XceI | RCATGY | 1 cut(s) | 760 |
| XspI | CTAG | 2 cut(s) | 125, 797 |