MD07G1221600.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001330
Physical Location & Seq
Forward (+)
29874100 .. 29876031
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1221600.v1.1.491

Sequence Viewer

Length: 627 bp
ATGTTTTGGGGGGAATTGTTAGATTACAAACTTAGATATTGGCTTGATGCTGCTGTAAAACCTTCTTTGGTGATGGTTTATTTTGGTGGTAATGATTCTATGGGGCCTCATCCATCCGGCCTCGGCCCTCATGTCCCGCTTCCTGAATATATCGAGAACATGAGAAAGATTGCAAGTCATCTGAAGAGCCTTTCAGATTCAACGCGGATCATCTTTCTTAGTTGTCCTCCAGTGAACGAGGCCGTAATAAATGAAAACATAAGCGGGAGTTTCAGCGAGATGATAAGAACCAACGAGTTATGCAAACAGTATTCAGAAGCTTGTATAAAGCTTTGCCAGGAAATCAATGTCACATCTGTTGATCTTTTCACTGCAGTTCAGAGAAAAGATGACTGGATGAATACTTGTTTTATAGATGGGATTCATTTTTCTGCTGAAGGGAGCAAGATAGTGGTGGAAGAGATACTCAAGGTACTGAGAGAAGCTGACTGGAAGCCTAGTTTACACTGGAAATCGATGCCAATAGAATTTTCAGAGGATTCACCATATGATATTGCTGCTGCTGACGGAAAGACAACGTTCAATCCCTCCAGCTCGACTTTTTATTGGGAGATTCAGTGGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

209

Amino Acids

23.68

Weight (kDa)

4.88

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL_2 PF13472 13 - 149 7.7e-15 GDSL-like Lipase/Acylhydrolase family
Lipase_GDSL PF00657 17 - 155 5.2e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 205
AciI CCGC 3 cut(s) 137, 205, 264
AclI AACGTT 1 cut(s) 578
AclWI GGATC 1 cut(s) 215
AcsI RAATTY 1 cut(s) 527
AcuI CTGAAG 2 cut(s) 203, 456
AfaI GTAC 1 cut(s) 474
AgsI TTSAA 2 cut(s) 201, 583
AjnI CCWGG 1 cut(s) 336
AjuI GAANNNNNNNTTGG 2 cut(s) 514, 546
AluBI AGCT 4 cut(s) 320, 331, 485, 594
AluI AGCT 4 cut(s) 320, 331, 485, 594
AlwI GGATC 1 cut(s) 215
AoxI GGCC 4 cut(s) 104, 118, 124, 240
ApeKI GCWGC 3 cut(s) 50, 557, 560
ApoI RAATTY 1 cut(s) 527
AspS9I GGNCC 2 cut(s) 104, 125
AsuHPI GGTGA 2 cut(s) 82, 534
BbvI GCAGC 3 cut(s) 37, 544, 547
BccI CCATC 3 cut(s) 67, 121, 410
BceAI ACGGC 1 cut(s) 227
BciT130I CCWGG 1 cut(s) 338
BfaI CTAG 1 cut(s) 498
BfmI CTRYAG 1 cut(s) 372
BisI GCNGC 3 cut(s) 51, 558, 561
BlsI GCNGC 3 cut(s) 52, 559, 562
Bme1390I CCNGG 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 104, 125
BmiI GGNNCC 1 cut(s) 105
BmrFI CCNGG 1 cut(s) 338
BmsI GCATC 2 cut(s) 37, 507
BpmI CTGGAG 2 cut(s) 213, 574
BpuEI CTTGAG 1 cut(s) 452
Bsa29I ATCGAT 1 cut(s) 515
BsaJI CCNNGG 1 cut(s) 121
BsaXI ACNNNNNCTCC 1 cut(s) 602
Bse1I ACTGG 4 cut(s) 230, 398, 494, 512
BseBI CCWGG 1 cut(s) 338
BseCI ATCGAT 1 cut(s) 515
BseDI CCNNGG 1 cut(s) 121
BseGI GGATG 3 cut(s) 109, 113, 402
BseMII CTCAG 1 cut(s) 467
BseNI ACTGG 4 cut(s) 230, 398, 494, 512
BseXI GCAGC 3 cut(s) 37, 544, 547
Bsh1236I CGCG 1 cut(s) 205
BshFI GGCC 4 cut(s) 106, 120, 126, 242
BshVI ATCGAT 1 cut(s) 515
BsiSI CCGG 1 cut(s) 117
BslFI GGGAC 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 4 cut(s) 106, 120, 126, 242
Bsp143I GATC 2 cut(s) 207, 361
BspACI CCGC 3 cut(s) 137, 205, 264
BspANI GGCC 4 cut(s) 106, 120, 126, 242
BspCNI CTCAG 1 cut(s) 468
BspDI ATCGAT 1 cut(s) 515
BspFNI CGCG 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 105
BspMAI CTGCAG 1 cut(s) 376
BspPI GGATC 1 cut(s) 215
BspQI GCTCTTC 1 cut(s) 179
BsrI ACTGG 4 cut(s) 230, 398, 494, 512
BssECI CCNNGG 1 cut(s) 121
BssMI GATC 2 cut(s) 207, 361
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 1 cut(s) 309
Bst6I CTCTTC 2 cut(s) 179, 453
BstDEI CTNAG 3 cut(s) 32, 218, 476
BstF5I GGATG 3 cut(s) 109, 113, 402
BstFNI CGCG 1 cut(s) 205
BstKTI GATC 2 cut(s) 210, 364
BstMBI GATC 2 cut(s) 207, 361
BstNI CCWGG 1 cut(s) 338
BstSCI CCNGG 1 cut(s) 336
BstSFI CTRYAG 1 cut(s) 372
BstUI CGCG 1 cut(s) 205
BstV1I GCAGC 3 cut(s) 37, 544, 547
Bsu15I ATCGAT 1 cut(s) 515
BsuRI GGCC 4 cut(s) 106, 120, 126, 242
BsuTUI ATCGAT 1 cut(s) 515
BtsCI GGATG 3 cut(s) 109, 113, 402
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 4 cut(s) 237, 369, 505, 623
Cfr13I GGNCC 2 cut(s) 104, 125
ClaI ATCGAT 1 cut(s) 515
Csp6I GTAC 1 cut(s) 473
CviAII CATG 2 cut(s) 131, 160
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 3 cut(s) 32, 218, 476
DpnI GATC 2 cut(s) 209, 363
DpnII GATC 2 cut(s) 207, 361
Eam1104I CTCTTC 2 cut(s) 179, 453
EarI CTCTTC 2 cut(s) 179, 453
Eco57I CTGAAG 2 cut(s) 203, 456
EcoO109I RGGNCCY 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 336
FaeI CATG 2 cut(s) 134, 163
FaqI GGGAC 1 cut(s) 119
FatI CATG 2 cut(s) 130, 159
FauI CCCGC 2 cut(s) 144, 257
FauNDI CATATG 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 51, 558, 561
FokI GGATG 3 cut(s) 96, 100, 409
Fsp4HI GCNGC 3 cut(s) 51, 558, 561
FspBI CTAG 1 cut(s) 498
GluI GCNGC 3 cut(s) 51, 558, 561
GsuI CTGGAG 2 cut(s) 213, 574
HaeIII GGCC 4 cut(s) 106, 120, 126, 242
HapII CCGG 1 cut(s) 117
Hin1II CATG 2 cut(s) 134, 163
HindIII AAGCTT 2 cut(s) 318, 329
HinfI GANTC 5 cut(s) 95, 197, 421, 539, 613
HpaII CCGG 1 cut(s) 117
HphI GGTGA 2 cut(s) 82, 534
Hpy166II GTNNAC 2 cut(s) 235, 503
Hpy188I TCNGA 5 cut(s) 183, 196, 316, 381, 535
Hpy188III TCNNGA 2 cut(s) 143, 154
Hpy8I GTNNAC 2 cut(s) 235, 503
HpyAV CCTTC 2 cut(s) 72, 431
HpyCH4III ACNGT 1 cut(s) 309
HpyCH4IV ACGT 1 cut(s) 578
HpyCH4V TGCA 3 cut(s) 173, 303, 374
HpyF3I CTNAG 3 cut(s) 32, 218, 476
HpySE526I ACGT 1 cut(s) 578
Hsp92II CATG 2 cut(s) 134, 163
Kzo9I GATC 2 cut(s) 207, 361
LguI GCTCTTC 1 cut(s) 179
LmnI GCTCC 1 cut(s) 441
LpnPI CCDG 9 cut(s) 130, 156, 243, 323, 350, 379, 475, 493, 604
Lsp1109I GCAGC 3 cut(s) 37, 544, 547
LweI GCATC 2 cut(s) 37, 507
MaeI CTAG 1 cut(s) 498
MaeII ACGT 1 cut(s) 578
MaeIII GTNAC 1 cut(s) 349
MalI GATC 2 cut(s) 209, 363
MboI GATC 2 cut(s) 207, 361
MboII GAAGA 2 cut(s) 196, 470
MluCI AATT 2 cut(s) 14, 527
MnlI CCTC 7 cut(s) 117, 131, 138, 232, 237, 529, 598
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 338
MvaI CCWGG 1 cut(s) 338
MvnI CGCG 1 cut(s) 205
NdeI CATATG 1 cut(s) 547
NdeII GATC 2 cut(s) 207, 361
NlaIII CATG 2 cut(s) 134, 163
NlaIV GGNNCC 1 cut(s) 105
NmeAIII GCCGAG 1 cut(s) 102
NmuCI GTSAC 1 cut(s) 349
PciSI GCTCTTC 1 cut(s) 179
PfeI GAWTC 5 cut(s) 95, 197, 421, 539, 613
PkrI GCNGC 3 cut(s) 52, 559, 562
Psp1406I AACGTT 1 cut(s) 578
Psp6I CCWGG 1 cut(s) 336
PspGI CCWGG 1 cut(s) 336
PspN4I GGNNCC 1 cut(s) 105
PspPI GGNCC 2 cut(s) 104, 125
PstI CTGCAG 1 cut(s) 376
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
SapI GCTCTTC 1 cut(s) 179
SatI GCNGC 3 cut(s) 51, 558, 561
Sau3AI GATC 2 cut(s) 207, 361
Sau96I GGNCC 2 cut(s) 104, 125
ScrFI CCNGG 1 cut(s) 338
SetI ASST 7 cut(s) 64, 322, 333, 474, 487, 581, 596
SfaNI GCATC 2 cut(s) 37, 507
SfcI CTRYAG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
Sse9I AATT 2 cut(s) 14, 527
SsiI CCGC 3 cut(s) 137, 205, 264
SspMI CTAG 1 cut(s) 498
StyD4I CCNGG 1 cut(s) 336
TaaI ACNGT 1 cut(s) 309
TaiI ACGT 1 cut(s) 581
TaqI TCGA 3 cut(s) 153, 515, 596
TasI AATT 2 cut(s) 14, 527
TfiI GAWTC 5 cut(s) 95, 197, 421, 539, 613
TscAI CASTG 4 cut(s) 237, 376, 512, 623
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 3 cut(s) 50, 557, 560
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 3 cut(s) 267, 413, 413
TspGWI ACGGA 1 cut(s) 582
TspRI CASTG 4 cut(s) 237, 376, 512, 623
XapI RAATTY 1 cut(s) 527
XspI CTAG 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.