pycom04g13820

lipid catabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001330
Physical Location & Seq
Forward (+)
16731527 .. 16733354
1828 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g13820.4

Sequence Viewer

Length: 786 bp
ATGGTCGGACCAACAAGACCTCAGTTTGTGATATTTGGCTCGTCCATTGTCCAGTTCAGCTTCGGCAATGGAGGATGGAGTGCCATTCTCGCTGACCTCTATGCTCGTAAGGCCGACGTACTGGTGCGAGGATACGCTGGTTGGAACTCGAGGCGTGCTTTGCTGGTTTTGGATCAAGTATTTCCCAAGGATGCGACAATTCAACCATCTTTGGTGATAGTCTATTTCGGTGGTAATGATTCGTTGGATCCTCATCCGTCAGGGCTAGGCGCTCATGTACCACTTCCTGAATATAAAGAAAATATGAGAAAGATTGGTAAACATTTAAAGAGTCTTTCAGAAAAAACTCGCGTTATCTTTCTTACTGCTCCTCCTGTCAACGAGGAACAAATTCATGAAGCCAGTGACTATGTTCCTCCAGAGAATCGAACAAACGAGTCCTGCAAAGCATATTCAGATGCTTGTTTAGACGTGTGCCGGGACTTGGATATCAAAGGTGTTGATTTATGGACTTCAATTCAAAAAAGAAAAGACTGGTCTACTACGTGCTTTACGGATGGAGTCCATTTTTCATCCGAAGGGAGCAAGATTGTGGGGGAGGAGATACTGAAGGTGCTTAGGGAAGCAGACTGGGAGCCCCGCCTACACTGGAAATCGTTGCCAACGGAATTTTCGGAGGATTCGCCTTATGATCCGCCCGCTGCTGATGGAAAAACAACTATAAACATCGCTGAGATCAGCGTTAATGAGGGTGTCAAGCATTGGGGACTAAGCAATGAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL_2

Protein Analysis

262

Amino Acids

29.06

Weight (kDa)

5.41

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 539
AccII CGCG 1 cut(s) 351
AciI CCGC 3 cut(s) 640, 695, 699
AclWI GGATC 4 cut(s) 180, 242, 255, 686
AcsI RAATTY 2 cut(s) 390, 668
AcuI CTGAAG 1 cut(s) 629
AfaI GTAC 2 cut(s) 120, 279
AfiI CCNNNNNNNGG 1 cut(s) 484
AflIII ACRYGT 1 cut(s) 471
AgsI TTSAA 3 cut(s) 203, 516, 521
AjiI CACGTC 1 cut(s) 472
AjuI GAANNNNNNNTTGG 2 cut(s) 655, 687
AluBI AGCT 1 cut(s) 60
AluI AGCT 1 cut(s) 60
AlwI GGATC 4 cut(s) 180, 242, 255, 686
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 1 cut(s) 111
ApeKI GCWGC 1 cut(s) 701
ApoI RAATTY 2 cut(s) 390, 668
Asp700I GAANNNNTTC 1 cut(s) 390
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 8
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 8
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BamHI GGATCC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 639
BbvI GCAGC 1 cut(s) 688
BccI CCATC 4 cut(s) 69, 214, 551, 701
BciVI GTATCC 1 cut(s) 125
BcnI CCSGG 1 cut(s) 479
BfaI CTAG 1 cut(s) 266
BfoI RGCGCY 1 cut(s) 273
BfuI GTATCC 1 cut(s) 125
BisI GCNGC 1 cut(s) 702
BlsI GCNGC 1 cut(s) 703
Bme1390I CCNGG 1 cut(s) 479
Bme18I GGWCC 1 cut(s) 8
BmeT110I CYCGRG 1 cut(s) 148
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 2 cut(s) 249, 636
BmrFI CCNGG 1 cut(s) 479
BmrI ACTGGG 1 cut(s) 640
BmsI GCATC 2 cut(s) 181, 448
BmuI ACTGGG 1 cut(s) 640
BpmI CTGGAG 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 617
BpuMI CCSGG 1 cut(s) 479
BsaAI YACGTR 1 cut(s) 546
BsaBI GATNNNNATC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 355, 385
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse1I ACTGG 6 cut(s) 52, 126, 402, 539, 635, 653
Bse3DI GCAATG 2 cut(s) 73, 781
Bse8I GATNNNNATC 1 cut(s) 252
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 5 cut(s) 80, 196, 253, 562, 572
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 484
BseMI GCAATG 2 cut(s) 73, 781
BseMII CTCAG 2 cut(s) 35, 723
BseNI ACTGG 6 cut(s) 52, 126, 402, 539, 635, 653
BseRI GAGGAG 2 cut(s) 360, 614
BseXI GCAGC 1 cut(s) 688
Bsh1236I CGCG 1 cut(s) 351
BshFI GGCC 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 1 cut(s) 478
BslFI GGGAC 2 cut(s) 494, 780
BslI CCNNNNNNNGG 1 cut(s) 484
BsmFI GGGAC 2 cut(s) 494, 780
BsnI GGCC 1 cut(s) 113
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 639
Bsp143I GATC 4 cut(s) 172, 247, 691, 735
BspACI CCGC 3 cut(s) 640, 695, 699
BspANI GGCC 1 cut(s) 113
BspCNI CTCAG 2 cut(s) 34, 724
BspFNI CGCG 1 cut(s) 351
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 2 cut(s) 249, 636
BspPI GGATC 4 cut(s) 180, 242, 255, 686
BsrDI GCAATG 2 cut(s) 73, 781
BsrI ACTGG 6 cut(s) 52, 126, 402, 539, 635, 653
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 4 cut(s) 172, 247, 691, 735
BssT1I CCWWGG 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 546
BstC8I GCNNGC 2 cut(s) 156, 699
BstDEI CTNAG 4 cut(s) 21, 617, 732, 770
BstF5I GGATG 5 cut(s) 80, 196, 253, 562, 572
BstFNI CGCG 1 cut(s) 351
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 4 cut(s) 175, 250, 694, 738
BstMBI GATC 4 cut(s) 172, 247, 691, 735
BstMWI GCNNNNNNNGC 3 cut(s) 89, 110, 160
BstSCI CCNGG 1 cut(s) 477
BstUI CGCG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 688
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuI GTATCC 1 cut(s) 125
BsuRI GGCC 1 cut(s) 113
BtgZI GCGATG 1 cut(s) 712
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 5 cut(s) 80, 196, 253, 562, 572
BtsIMutI CAGTG 2 cut(s) 409, 646
Cac8I GCNNGC 2 cut(s) 156, 699
CciI TCATGA 1 cut(s) 394
CfoI GCGC 1 cut(s) 272
Cfr13I GGNCC 1 cut(s) 8
Csp6I GTAC 2 cut(s) 119, 278
CviAII CATG 2 cut(s) 275, 395
CviJI RGCY 6 cut(s) 39, 60, 113, 265, 401, 637
CviKI_1 RGCY 6 cut(s) 39, 60, 113, 265, 401, 637
CviQI GTAC 2 cut(s) 119, 278
DdeI CTNAG 4 cut(s) 21, 617, 732, 770
DpnI GATC 4 cut(s) 174, 249, 693, 737
DpnII GATC 4 cut(s) 172, 247, 691, 735
DraI TTTAAA 1 cut(s) 327
EciI GGCGGA 1 cut(s) 684
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 639
Eco32I GATATC 1 cut(s) 490
Eco47I GGWCC 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 629
Eco88I CYCGRG 1 cut(s) 148
EcoRV GATATC 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 639
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 2 cut(s) 278, 398
FaqI GGGAC 2 cut(s) 494, 780
FatI CATG 2 cut(s) 274, 394
FauI CCCGC 2 cut(s) 647, 706
FblI GTMKAC 1 cut(s) 539
Fnu4HI GCNGC 1 cut(s) 702
FokI GGATG 5 cut(s) 87, 203, 240, 559, 569
FriOI GRGCYC 1 cut(s) 639
Fsp4HI GCNGC 1 cut(s) 702
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 271
GluI GCNGC 1 cut(s) 702
GsuI CTGGAG 1 cut(s) 402
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 1 cut(s) 113
HapII CCGG 1 cut(s) 478
HhaI GCGC 1 cut(s) 272
Hin1II CATG 2 cut(s) 278, 398
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 6 cut(s) 239, 331, 424, 437, 561, 680
HpaII CCGG 1 cut(s) 478
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 3 cut(s) 320, 379, 540
Hpy188I TCNGA 5 cut(s) 8, 340, 457, 577, 676
Hpy188III TCNNGA 3 cut(s) 287, 395, 419
Hpy8I GTNNAC 3 cut(s) 320, 379, 540
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 2 cut(s) 572, 604
HpyCH4IV ACGT 3 cut(s) 117, 471, 545
HpyCH4V TGCA 1 cut(s) 444
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 110, 160
HpyF3I CTNAG 4 cut(s) 21, 617, 732, 770
HpySE526I ACGT 3 cut(s) 117, 471, 545
Hsp92II CATG 2 cut(s) 278, 398
HspAI GCGC 1 cut(s) 270
Kzo9I GATC 4 cut(s) 172, 247, 691, 735
LmnI GCTCC 3 cut(s) 373, 582, 634
Lsp1109I GCAGC 1 cut(s) 688
LweI GCATC 2 cut(s) 181, 448
MaeI CTAG 1 cut(s) 266
MaeII ACGT 3 cut(s) 117, 471, 545
MaeIII GTNAC 1 cut(s) 404
MalI GATC 4 cut(s) 174, 249, 693, 737
MboI GATC 4 cut(s) 172, 247, 691, 735
MflI RGATCY 1 cut(s) 247
MhlI GDGCHC 1 cut(s) 639
MluCI AATT 4 cut(s) 198, 390, 516, 668
MlyI GAGTC 3 cut(s) 340, 446, 570
MmeI TCCRAC 2 cut(s) 122, 225
MroXI GAANNNNTTC 1 cut(s) 390
MseI TTAA 3 cut(s) 326, 744, 784
MspA1I CMGCKG 1 cut(s) 701
MspI CCGG 1 cut(s) 478
MspR9I CCNGG 1 cut(s) 479
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 3 cut(s) 89, 110, 160
NciI CCSGG 1 cut(s) 479
NdeII GATC 4 cut(s) 172, 247, 691, 735
NlaIII CATG 2 cut(s) 278, 398
NlaIV GGNNCC 2 cut(s) 249, 636
NmuCI GTSAC 1 cut(s) 404
PaeR7I CTCGAG 1 cut(s) 148
PagI TCATGA 1 cut(s) 394
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 3 cut(s) 239, 424, 680
PkrI GCNGC 1 cut(s) 703
PleI GAGTC 3 cut(s) 339, 445, 569
PpsI GAGTC 3 cut(s) 339, 445, 569
Ppu21I YACGTR 1 cut(s) 546
PspN4I GGNNCC 2 cut(s) 249, 636
PspPI GGNCC 1 cut(s) 8
PspXI VCTCGAGB 1 cut(s) 148
PsuI RGATCY 1 cut(s) 247
RsaI GTAC 2 cut(s) 120, 279
RsaNI GTAC 2 cut(s) 119, 278
SaqAI TTAA 3 cut(s) 326, 744, 784
SatI GCNGC 1 cut(s) 702
Sau3AI GATC 4 cut(s) 172, 247, 691, 735
Sau96I GGNCC 1 cut(s) 8
SchI GAGTC 3 cut(s) 340, 446, 570
ScrFI CCNGG 1 cut(s) 479
SduI GDGCHC 1 cut(s) 639
SetI ASST 8 cut(s) 22, 62, 99, 120, 474, 499, 548, 615
SfaNI GCATC 2 cut(s) 181, 448
Sfr274I CTCGAG 1 cut(s) 148
SinI GGWCC 1 cut(s) 8
SlaI CTCGAG 1 cut(s) 148
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 4 cut(s) 198, 390, 516, 668
SsiI CCGC 3 cut(s) 640, 695, 699
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 186
TaiI ACGT 3 cut(s) 120, 474, 548
TaqI TCGA 2 cut(s) 149, 427
TasI AATT 4 cut(s) 198, 390, 516, 668
TfiI GAWTC 3 cut(s) 239, 424, 680
Tru1I TTAA 3 cut(s) 326, 744, 784
Tru9I TTAA 3 cut(s) 326, 744, 784
TscAI CASTG 2 cut(s) 409, 653
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 1 cut(s) 701
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 3 cut(s) 383, 411, 561
TspGWI ACGGA 3 cut(s) 246, 569, 680
TspRI CASTG 2 cut(s) 409, 653
VpaK11BI GGWCC 1 cut(s) 8
XapI RAATTY 2 cut(s) 390, 668
XhoI CTCGAG 1 cut(s) 148
XmiI GTMKAC 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.