FvH4_4g22020

Histone-lysine N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002127
Physical Location & Seq
Forward (+)
24943633 .. 24945202
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22020.t1

Sequence Viewer

Length: 1191 bp
ATGGCAATGACAAAGGAGCTGAGGCTAACGGAGTTACAAGGCAGAGGAAGGTCATTAGTGGCGGCGCAGCCACTCAAGAGCGGCCAAATCGTTCTCCGCGATTCTCCCATACTCCTCTACTCCGCTCTTCCTCTCCTCACTCCCTCCCCTCCCCCTTACTGCGACCACTGCTTCAAAACCCTAAACCCCCAAACCCCAATCTCCCTCTGCCCCTCCTGCTCCCACCACCGCTTCTGCAGCCCCAACTGCCTCTCCGCCGCCCTCTCCTCCTCCCACACTCCATGGGTCTGCCAATCCCTCGCCAAATTACGAAACTGCCCCTCCCCTCTCTCCGCCCACCCCGCCGATCGCCAAGTCCAAGCTCGCTTCTTGATCGCTGCTTACAACCTCGCCGCCGTCTCGCCGTCGAGTTTCCAGCTGCTGCTAACTCTCCAGGGCGAACCGCAGGACTCCGCCGAAGCTCAGTTCCTGCATTCGCTGATTTCGACTCTCTGCCCTCCTCCGCAGCTGTTCTCGGTGGAGCTCACGGCGGCGCTGCTGGCTAAGGATAAGCTCAATGCGTTTGGATTGATGGAGCCTCCTTCGGAAACTGGGCTGAGGTCGAGCAGAGCTTATGGGATTTATCCCAAGGCCTCGTTCTTCAACCATGATTGTCTCCCCAATGCGTGCCGATTTGATTACGTCGATGAAGGGAGCACGGAGATTACTGTGAGGATGATTCATGATGTTCCGGAGGGGAGGGAGATTTGCTTGAGCTATTTCCCTGTTAATGAGCCTTATTCGAGCCGGCAGAGGACTTTGGCTGAGGACTATGGATTTGTGTGCCAATGTGATCGGTGCAAGGTTGAAGCGAATTGGTCTGACAATGAGGAGGTGGCTGAGGAGAAAGGTGGTTCGGCTGAGGGGATGGATGAGGACCAAGACCAGGAAATGGGAGGAGATTTGGAAGCAGAAGGTGGGAGTGGGAGTGACATTGAAGCTGAAGCGCAAGGAGAGGCTGATTTTCCACATGCCTATTTCTTTGTGAGATTTATGTGTAGTAGAACCAATTGCTGGGGCACGCTAGCCCCTTTGCCCCCGAAAGATGATGGAACTTCTTCGGATGCTATGGAATGCAATGCGTGTGGAAGCCTTAAGCAAGATGAGGAGATTAGTGGTCATGGACAAAATGGGATCTCTTTGGATGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SET

Protein Analysis

397

Amino Acids

43.14

Weight (kDa)

4.75

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 16 - 253 3.6e-08 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 931, 1053
AccBSI CCGCTC 2 cut(s) 81, 125
AccII CGCG 1 cut(s) 99
AccIII TCCGGA 1 cut(s) 730
AclWI GGATC 1 cut(s) 1181
AcoI YGGCCR 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 1002
AfiI CCNNNNNNNGG 3 cut(s) 925, 931, 1053
AflII CTTAAG 1 cut(s) 1133
AgsI TTSAA 4 cut(s) 175, 643, 848, 977
AjnI CCWGG 2 cut(s) 432, 924
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
Alw21I GWGCWC 2 cut(s) 525, 698
Alw26I GTCTC 2 cut(s) 403, 659
AlwI GGATC 1 cut(s) 1181
AlwNI CAGNNNCTG 2 cut(s) 421, 469
Aor13HI TCCGGA 1 cut(s) 730
AoxI GGCC 2 cut(s) 82, 630
ApeKI GCWGC 7 cut(s) 67, 237, 377, 418, 421, 505, 535
ArsI GACNNNNNNTTYG 2 cut(s) 800, 832
Asp700I GAANNNNTTC 1 cut(s) 1096
AspLEI GCGC 3 cut(s) 67, 535, 988
AspS9I GGNCC 1 cut(s) 916
AsuNHI GCTAGC 1 cut(s) 1063
AvaII GGWCC 1 cut(s) 916
BaeGI GKGCMC 1 cut(s) 1061
BanII GRGCYC 1 cut(s) 525
Bbv12I GWGCWC 2 cut(s) 525, 698
BbvCI CCTCAGC 5 cut(s) 20, 596, 804, 879, 900
BbvI GCAGC 7 cut(s) 79, 249, 364, 405, 408, 517, 522
BccI CCATC 4 cut(s) 565, 901, 1082, 1178
BceAI ACGGC 3 cut(s) 380, 388, 543
BciT130I CCWGG 2 cut(s) 434, 926
BcoDI GTCTC 2 cut(s) 403, 659
BfaI CTAG 2 cut(s) 1064, 1189
BfmI CTRYAG 1 cut(s) 235
BfoI RGCGCY 1 cut(s) 536
BfrI CTTAAG 1 cut(s) 1133
Bme1390I CCNGG 2 cut(s) 434, 926
Bme18I GGWCC 1 cut(s) 916
BmgT120I GGNCC 1 cut(s) 916
BmiI GGNNCC 1 cut(s) 576
BmrFI CCNGG 2 cut(s) 434, 926
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 1 cut(s) 1093
BmtI GCTAGC 1 cut(s) 1067
BmuI ACTGGG 1 cut(s) 600
BpmI CTGGAG 1 cut(s) 416
Bpu10I CCTNAGC 6 cut(s) 20, 543, 596, 804, 879, 900
BpuEI CTTGAG 2 cut(s) 59, 772
BsaJI CCNNGG 3 cut(s) 281, 433, 627
BsaWI WCCGGW 1 cut(s) 730
BsaXI ACNNNNNCTCC 6 cut(s) 236, 266, 305, 335, 952, 982
Bsc4I CCNNNNNNNGG 3 cut(s) 925, 931, 1053
Bse118I RCCGGY 1 cut(s) 786
Bse1I ACTGG 1 cut(s) 595
Bse3DI GCAATG 2 cut(s) 12, 1123
BseAI TCCGGA 1 cut(s) 730
BseBI CCWGG 2 cut(s) 434, 926
BseDI CCNNGG 3 cut(s) 281, 433, 627
BseGI GGATG 5 cut(s) 720, 912, 916, 1108, 1189
BseLI CCNNNNNNNGG 3 cut(s) 925, 931, 1053
BseMI GCAATG 2 cut(s) 12, 1123
BseMII CTCAG 6 cut(s) 11, 476, 587, 795, 870, 891
BseNI ACTGG 1 cut(s) 595
BseRI GAGGAG 9 cut(s) 104, 125, 256, 259, 489, 884, 896, 951, 1160
BseSI GKGCMC 1 cut(s) 1061
BseXI GCAGC 7 cut(s) 79, 249, 364, 405, 408, 517, 522
BseYI CCCAGC 1 cut(s) 1053
Bsh1236I CGCG 1 cut(s) 99
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 2 cut(s) 84, 632
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 2 cut(s) 525, 698
BsiSI CCGG 2 cut(s) 731, 787
BslI CCNNNNNNNGG 3 cut(s) 925, 931, 1053
BsmAI GTCTC 2 cut(s) 403, 659
BsmBI CGTCTC 1 cut(s) 403
BsmI GAATGC 2 cut(s) 472, 1118
BsnI GGCC 2 cut(s) 84, 632
Bsp1286I GDGCHC 3 cut(s) 525, 698, 1061
Bsp13I TCCGGA 1 cut(s) 730
Bsp143I GATC 4 cut(s) 346, 372, 832, 1173
Bsp19I CCATGG 1 cut(s) 281
BspANI GGCC 2 cut(s) 84, 632
BspCNI CTCAG 6 cut(s) 12, 475, 588, 796, 871, 892
BspEI TCCGGA 1 cut(s) 730
BspFNI CGCG 1 cut(s) 99
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 1 cut(s) 576
BspMAI CTGCAG 1 cut(s) 239
BspOI GCTAGC 1 cut(s) 1067
BspPI GGATC 1 cut(s) 1181
BspQI GCTCTTC 1 cut(s) 132
BspTI CTTAAG 1 cut(s) 1133
BsrBI CCGCTC 2 cut(s) 81, 125
BsrDI GCAATG 2 cut(s) 12, 1123
BsrFI RCCGGY 1 cut(s) 786
BsrI ACTGG 1 cut(s) 595
BssAI RCCGGY 1 cut(s) 786
BssECI CCNNGG 3 cut(s) 281, 433, 627
BssMI GATC 4 cut(s) 346, 372, 832, 1173
BssT1I CCWWGG 2 cut(s) 281, 627
Bst2UI CCWGG 2 cut(s) 434, 926
Bst4CI ACNGT 1 cut(s) 709
Bst6I CTCTTC 1 cut(s) 132
BstAFI CTTAAG 1 cut(s) 1133
BstC8I GCNNGC 6 cut(s) 364, 540, 667, 788, 1061, 1065
BstDEI CTNAG 7 cut(s) 20, 462, 543, 596, 804, 879, 900
BstDSI CCRYGG 1 cut(s) 281
BstF5I GGATG 5 cut(s) 720, 912, 916, 1108, 1189
BstFNI CGCG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 536
BstHHI GCGC 3 cut(s) 67, 535, 988
BstKTI GATC 4 cut(s) 349, 375, 835, 1176
BstMAI GTCTC 2 cut(s) 403, 659
BstMBI GATC 4 cut(s) 346, 372, 832, 1173
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 6 cut(s) 168, 216, 237, 246, 341, 539
BstNI CCWGG 2 cut(s) 434, 926
BstNSI RCATGY 1 cut(s) 1013
BstSCI CCNGG 2 cut(s) 432, 924
BstSFI CTRYAG 1 cut(s) 235
BstSLI GKGCMC 1 cut(s) 1061
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 7 cut(s) 79, 249, 364, 405, 408, 517, 522
BstX2I RGATCY 1 cut(s) 1173
BstYI RGATCY 1 cut(s) 1173
BsuRI GGCC 2 cut(s) 84, 632
BtgI CCRYGG 1 cut(s) 281
BtsCI GGATG 5 cut(s) 720, 912, 916, 1108, 1189
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 6 cut(s) 364, 540, 667, 788, 1061, 1065
CaiI CAGNNNCTG 2 cut(s) 421, 469
CciI TCATGA 1 cut(s) 721
CfoI GCGC 3 cut(s) 67, 535, 988
Cfr10I RCCGGY 1 cut(s) 786
Cfr13I GGNCC 1 cut(s) 916
CspCI CAANNNNNGTGG 2 cut(s) 1105, 1140
CviAII CATG 5 cut(s) 282, 647, 722, 1010, 1160
DdeI CTNAG 7 cut(s) 20, 462, 543, 596, 804, 879, 900
DpnI GATC 4 cut(s) 348, 374, 834, 1175
DpnII GATC 4 cut(s) 346, 372, 832, 1173
EaeI YGGCCR 1 cut(s) 82
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
EciI GGCGGA 3 cut(s) 244, 322, 442
Ecl136II GAGCTC 1 cut(s) 523
Eco130I CCWWGG 2 cut(s) 281, 627
Eco147I AGGCCT 1 cut(s) 632
Eco24I GRGCYC 1 cut(s) 525
Eco47I GGWCC 1 cut(s) 916
Eco53kI GAGCTC 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 1002
EcoICRI GAGCTC 1 cut(s) 523
EcoRII CCWGG 2 cut(s) 432, 924
EcoT14I CCWWGG 2 cut(s) 281, 627
EcoT38I GRGCYC 1 cut(s) 525
ErhI CCWWGG 2 cut(s) 281, 627
Esp3I CGTCTC 1 cut(s) 403
FaeI CATG 5 cut(s) 285, 650, 725, 1013, 1163
FatI CATG 5 cut(s) 281, 646, 721, 1009, 1159
FauI CCCGC 1 cut(s) 349
FokI GGATG 4 cut(s) 727, 919, 923, 1115
FriOI GRGCYC 1 cut(s) 525
FspBI CTAG 2 cut(s) 1064, 1189
GlaI GCGC 3 cut(s) 66, 534, 987
GsaI CCCAGC 1 cut(s) 1057
GsuI CTGGAG 1 cut(s) 416
HaeII RGCGCY 1 cut(s) 536
HaeIII GGCC 2 cut(s) 84, 632
HapII CCGG 2 cut(s) 731, 787
HhaI GCGC 3 cut(s) 67, 535, 988
Hin1II CATG 5 cut(s) 285, 650, 725, 1013, 1163
Hin6I GCGC 3 cut(s) 65, 533, 986
HinP1I GCGC 3 cut(s) 65, 533, 986
HinfI GANTC 4 cut(s) 101, 449, 487, 718
HpaII CCGG 2 cut(s) 731, 787
Hpy188I TCNGA 3 cut(s) 586, 862, 1102
Hpy188III TCNNGA 4 cut(s) 76, 370, 722, 731
Hpy99I CGWCG 2 cut(s) 409, 686
HpyAV CCTTC 4 cut(s) 42, 591, 683, 947
HpyCH4III ACNGT 1 cut(s) 709
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 4 cut(s) 237, 472, 840, 1116
HpyF10VI GCNNNNNNNGC 6 cut(s) 168, 216, 237, 246, 341, 539
HpyF3I CTNAG 7 cut(s) 20, 462, 543, 596, 804, 879, 900
HpySE526I ACGT 1 cut(s) 681
Hsp92II CATG 5 cut(s) 285, 650, 725, 1013, 1163
HspAI GCGC 3 cut(s) 65, 533, 986
Kpn2I TCCGGA 1 cut(s) 730
KroI GCCGGC 1 cut(s) 786
KroNI GCCGGC 1 cut(s) 788
Kzo9I GATC 4 cut(s) 346, 372, 832, 1173
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 5 cut(s) 16, 224, 520, 574, 693
Lsp1109I GCAGC 7 cut(s) 79, 249, 364, 405, 408, 517, 522
LweI GCATC 1 cut(s) 1093
MaeI CTAG 2 cut(s) 1064, 1189
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 2 cut(s) 33, 968
MalI GATC 4 cut(s) 348, 374, 834, 1175
MbiI CCGCTC 2 cut(s) 81, 125
MboI GATC 4 cut(s) 346, 372, 832, 1173
MboII GAAGA 3 cut(s) 119, 631, 1089
MfeI CAATTG 1 cut(s) 1048
MflI RGATCY 1 cut(s) 1173
MhlI GDGCHC 3 cut(s) 525, 698, 1061
MluCI AATT 3 cut(s) 305, 853, 1048
MlyI GAGTC 2 cut(s) 443, 481
MroI TCCGGA 1 cut(s) 730
MroNI GCCGGC 1 cut(s) 786
MroXI GAANNNNTTC 1 cut(s) 1096
MseI TTAA 2 cut(s) 768, 1134
MspA1I CMGCKG 2 cut(s) 418, 508
MspCI CTTAAG 1 cut(s) 1133
MspI CCGG 2 cut(s) 731, 787
MspR9I CCNGG 2 cut(s) 434, 926
MunI CAATTG 1 cut(s) 1048
Mva1269I GAATGC 2 cut(s) 472, 1118
MvaI CCWGG 2 cut(s) 434, 926
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 6 cut(s) 168, 216, 237, 246, 341, 539
NaeI GCCGGC 1 cut(s) 788
NcoI CCATGG 1 cut(s) 281
NdeII GATC 4 cut(s) 346, 372, 832, 1173
NgoMIV GCCGGC 1 cut(s) 786
NheI GCTAGC 1 cut(s) 1063
NlaIII CATG 5 cut(s) 285, 650, 725, 1013, 1163
NlaIV GGNNCC 1 cut(s) 576
NmuCI GTSAC 1 cut(s) 968
NspI RCATGY 1 cut(s) 1013
PagI TCATGA 1 cut(s) 721
PceI AGGCCT 1 cut(s) 632
PciSI GCTCTTC 1 cut(s) 132
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 482
PctI GAATGC 2 cut(s) 472, 1118
PdiI GCCGGC 1 cut(s) 788
PdmI GAANNNNTTC 1 cut(s) 1096
PfeI GAWTC 2 cut(s) 101, 718
PflMI CCANNNNNTGG 2 cut(s) 931, 1053
Ple19I CGATCG 1 cut(s) 349
PleI GAGTC 2 cut(s) 443, 481
PpsI GAGTC 2 cut(s) 443, 481
Psp124BI GAGCTC 1 cut(s) 525
Psp6I CCWGG 2 cut(s) 432, 924
PspFI CCCAGC 1 cut(s) 1053
PspGI CCWGG 2 cut(s) 432, 924
PspN4I GGNNCC 1 cut(s) 576
PspPI GGNCC 1 cut(s) 916
PstI CTGCAG 1 cut(s) 239
PstNI CAGNNNCTG 2 cut(s) 421, 469
PsuI RGATCY 1 cut(s) 1173
PvuI CGATCG 1 cut(s) 349
PvuII CAGCTG 2 cut(s) 418, 508
SacI GAGCTC 1 cut(s) 525
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 2 cut(s) 768, 1134
Sau3AI GATC 4 cut(s) 346, 372, 832, 1173
Sau96I GGNCC 1 cut(s) 916
SchI GAGTC 2 cut(s) 443, 481
ScrFI CCNGG 2 cut(s) 434, 926
SduI GDGCHC 3 cut(s) 525, 698, 1061
SfaNI GCATC 1 cut(s) 1093
SfcI CTRYAG 1 cut(s) 235
SinI GGWCC 1 cut(s) 916
SmlI CTYRAG 3 cut(s) 74, 751, 1133
SmoI CTYRAG 3 cut(s) 74, 751, 1133
Sse9I AATT 3 cut(s) 305, 853, 1048
SseBI AGGCCT 1 cut(s) 632
SspMI CTAG 2 cut(s) 1064, 1189
SstI GAGCTC 1 cut(s) 525
StuI AGGCCT 1 cut(s) 632
StyD4I CCNGG 2 cut(s) 432, 924
StyI CCWWGG 2 cut(s) 281, 627
TaaI ACNGT 1 cut(s) 709
TaiI ACGT 1 cut(s) 684
TaqI TCGA 5 cut(s) 407, 485, 602, 684, 782
TasI AATT 3 cut(s) 305, 853, 1048
TauI GCSGC 5 cut(s) 65, 84, 260, 395, 533
TfiI GAWTC 2 cut(s) 101, 718
Tru1I TTAA 2 cut(s) 768, 1134
Tru9I TTAA 2 cut(s) 768, 1134
TscAI CASTG 1 cut(s) 173
TseFI GTSAC 1 cut(s) 968
TseI GCWGC 7 cut(s) 67, 237, 377, 418, 421, 505, 535
Tsp45I GTSAC 1 cut(s) 968
TspDTI ATGAA 2 cut(s) 702, 710
TspGWI ACGGA 2 cut(s) 44, 713
TspRI CASTG 1 cut(s) 173
Van91I CCANNNNNTGG 2 cut(s) 931, 1053
Vha464I CTTAAG 1 cut(s) 1133
VpaK11BI GGWCC 1 cut(s) 916
XceI RCATGY 1 cut(s) 1013
XmnI GAANNNNTTC 1 cut(s) 1096
XspI CTAG 2 cut(s) 1064, 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.