Rroxscaffold_5G00367650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002127
Physical Location & Seq
Forward (+)
49479576 .. 49484303
4728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367650.1

Sequence Viewer

Length: 465 bp
ATGGCCACTTCGGATTCCAAGGTGGAATTCGAGGGGTCCGATGATGAAGACTACTCCTCTGCTCGAAAAGAACCCCTTCCTCCCGGTGCGAAAGCGAAGAGCAAAGCCCGCCAGCACGCGAAATCGAAGAGAAAGGAGAAATTGAAACAGGAGGAGAAATCGAACACGCCCTCGTCCGAGGAAACTGGTGATACTAGCTTTTATGATTCAGACTCGAAATTCTTTATGGACGAGAATTATTTGGCTGCTATGAACAAAGAGCGCCTTCTGGGGCTTGGGCCTGATCAAGAAATGTATCCTCTTCCGGTGACTGTACAAGAATGTATTGCCTTTTTTGATATAGATGTTGAGGAATATGAGTTGATGGCAGATAGAGTGCGTAAGGATTGCTCCCTTAGTTCTTACATAGAAGATTTTCTGTCAATTTCCTTGCAGCACGAACCCAGTTGGCAACTAGCAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

17.54

Weight (kDa)

4.63

Isoelectric Point (pI)

56.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 119
AciI CCGC 1 cut(s) 109
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 26, 218
AfaI GTAC 1 cut(s) 315
AgsI TTSAA 1 cut(s) 145
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AluBI AGCT 1 cut(s) 198
AluI AGCT 1 cut(s) 198
AoxI GGCC 2 cut(s) 3, 278
ApeKI GCWGC 2 cut(s) 245, 433
ApoI RAATTY 2 cut(s) 26, 218
Asp700I GAANNNNTTC 2 cut(s) 75, 414
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 2 cut(s) 36, 278
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 2 cut(s) 200, 319
AvaII GGWCC 1 cut(s) 36
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 54
BbvI GCAGC 2 cut(s) 232, 445
BccI CCATC 1 cut(s) 358
BciVI GTATCC 1 cut(s) 306
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 1 cut(s) 84
BfaI CTAG 3 cut(s) 195, 455, 463
BfoI RGCGCY 1 cut(s) 265
BfuI GTATCC 1 cut(s) 306
BisI GCNGC 2 cut(s) 246, 434
BlsI GCNGC 2 cut(s) 247, 435
Bme1390I CCNGG 1 cut(s) 84
Bme18I GGWCC 1 cut(s) 36
BmgT120I GGNCC 2 cut(s) 36, 278
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 1 cut(s) 84
BmrI ACTGGG 1 cut(s) 438
BmuI ACTGGG 1 cut(s) 438
BpiI GAAGAC 1 cut(s) 54
BpuMI CCSGG 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 18, 177
BsaWI WCCGGW 1 cut(s) 304
Bse1I ACTGG 2 cut(s) 190, 444
BseDI CCNNGG 2 cut(s) 18, 177
BseNI ACTGG 2 cut(s) 190, 444
BseRI GAGGAG 2 cut(s) 46, 167
BseXI GCAGC 2 cut(s) 232, 445
Bsh1236I CGCG 1 cut(s) 119
BshFI GGCC 2 cut(s) 5, 280
BsiSI CCGG 2 cut(s) 84, 305
BsnI GGCC 2 cut(s) 5, 280
Bsp1407I TGTACA 1 cut(s) 313
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 1 cut(s) 109
BspANI GGCC 2 cut(s) 5, 280
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 1 cut(s) 37
BspQI GCTCTTC 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 313
BsrI ACTGG 2 cut(s) 190, 444
BssECI CCNNGG 2 cut(s) 18, 177
BssMI GATC 1 cut(s) 283
BssT1I CCWWGG 1 cut(s) 18
Bst4CI ACNGT 1 cut(s) 313
Bst6I CTCTTC 3 cut(s) 92, 122, 306
BstAUI TGTACA 1 cut(s) 313
BstC8I GCNNGC 3 cut(s) 109, 113, 117
BstDEI CTNAG 1 cut(s) 395
BstFNI CGCG 1 cut(s) 119
BstH2I RGCGCY 1 cut(s) 265
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 1 cut(s) 286
BstMBI GATC 1 cut(s) 283
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 82
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 2 cut(s) 232, 445
BstV2I GAAGAC 1 cut(s) 54
BsuI GTATCC 1 cut(s) 306
BsuRI GGCC 2 cut(s) 5, 280
Cac8I GCNNGC 3 cut(s) 109, 113, 117
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 2 cut(s) 36, 278
Csp6I GTAC 1 cut(s) 314
CviJI RGCY 6 cut(s) 5, 107, 198, 245, 274, 280
CviKI_1 RGCY 6 cut(s) 5, 107, 198, 245, 274, 280
CviQI GTAC 1 cut(s) 314
DdeI CTNAG 1 cut(s) 395
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 92, 122, 306
EarI CTCTTC 3 cut(s) 92, 122, 306
Eco130I CCWWGG 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 36
EcoRI GAATTC 1 cut(s) 26
EcoT14I CCWWGG 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 18
FaiI YATR 6 cut(s) 204, 227, 251, 341, 357, 407
FalI AAGNNNNNCTT 4 cut(s) 60, 92, 249, 281
FauI CCCGC 1 cut(s) 116
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 2 cut(s) 246, 434
Fsp4HI GCNGC 2 cut(s) 246, 434
FspBI CTAG 3 cut(s) 195, 455, 463
GlaI GCGC 1 cut(s) 263
GluI GCNGC 2 cut(s) 246, 434
HaeII RGCGCY 1 cut(s) 265
HaeIII GGCC 2 cut(s) 5, 280
HapII CCGG 2 cut(s) 84, 305
HhaI GCGC 1 cut(s) 264
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HinfI GANTC 3 cut(s) 14, 206, 212
HpaII CCGG 2 cut(s) 84, 305
HphI GGTGA 2 cut(s) 200, 319
Hpy188I TCNGA 4 cut(s) 13, 40, 178, 211
Hpy188III TCNNGA 1 cut(s) 287
HpyAV CCTTC 2 cut(s) 86, 275
HpyCH4III ACNGT 1 cut(s) 313
HpyCH4V TGCA 1 cut(s) 433
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 1 cut(s) 395
HspAI GCGC 1 cut(s) 262
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 1 cut(s) 283
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 1 cut(s) 395
LpnPI CCDG 8 cut(s) 97, 125, 134, 171, 254, 294, 318, 457
Lsp1109I GCAGC 2 cut(s) 232, 445
MaeI CTAG 3 cut(s) 195, 455, 463
MaeIII GTNAC 1 cut(s) 307
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 5 cut(s) 59, 109, 139, 293, 422
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 26, 140, 218, 235, 423
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 8 cut(s) 25, 67, 90, 145, 172, 181, 309, 343
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 75, 414
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 84, 305
MspR9I CCNGG 1 cut(s) 84
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 108
NciI CCSGG 1 cut(s) 84
NdeII GATC 1 cut(s) 283
NlaIV GGNNCC 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 92
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdmI GAANNNNTTC 2 cut(s) 75, 414
PfeI GAWTC 2 cut(s) 14, 206
PkrI GCNGC 2 cut(s) 247, 435
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PspN4I GGNNCC 1 cut(s) 37
PspPI GGNCC 2 cut(s) 36, 278
RsaI GTAC 1 cut(s) 315
RsaNI GTAC 1 cut(s) 314
SapI GCTCTTC 1 cut(s) 92
SatI GCNGC 2 cut(s) 246, 434
Sau3AI GATC 1 cut(s) 283
Sau96I GGNCC 2 cut(s) 36, 278
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 1 cut(s) 84
SetI ASST 2 cut(s) 24, 200
SinI GGWCC 1 cut(s) 36
Sse9I AATT 5 cut(s) 26, 140, 218, 235, 423
SsiI CCGC 1 cut(s) 109
SspMI CTAG 3 cut(s) 195, 455, 463
StyD4I CCNGG 1 cut(s) 82
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 1 cut(s) 313
TaqI TCGA 5 cut(s) 30, 64, 125, 161, 215
TasI AATT 5 cut(s) 26, 140, 218, 235, 423
TatI WGTACW 1 cut(s) 313
TfiI GAWTC 2 cut(s) 14, 206
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 2 cut(s) 245, 433
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 2 cut(s) 60, 266
VpaK11BI GGWCC 1 cut(s) 36
XapI RAATTY 2 cut(s) 26, 218
XmnI GAANNNNTTC 2 cut(s) 75, 414
XspI CTAG 3 cut(s) 195, 455, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.