Rorug04G0223900

Histone-lysine N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002127
Physical Location & Seq
Reverse (-)
38415024 .. 38416147
1124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0223900.1

Sequence Viewer

Length: 618 bp
ATGGCCATGGCAACTCAAGCGACCCTCTTCACCCCAACCGCCTCAGCACCATGGAAACAGTCAGTCACCTCCACCTTCATGACCCCAAAGTCACTGACCCTGTCCACCGCAGCGCCAAGACCCATGAGGATCGCAGCTGCTGAAGAGAAAACCGTGGCACCCACAAAGGAGGCCGAAGCTCCGGTTGGTTTCACCCCGCCGGAACTGGACCCAACAACCCCATCCCCAATCTTCGGCGGCAGCACCGGTGGGCTTCTCCGCAAGGCCCAAGAGGAAGAGTTCTACGTGATCACCTGGGAATCACCCAAGGAGCAAATCTTTGAGATGCCAACTGGTGGTGCAGCCATCATGAGGGAAGGACCTAACTTGTTGAAGCTGGCCAGGAAAGAGCAGTGTTTGGCTCTTGGGACTAGACTGAGGTCCAAGTACAAGATCAAGTACCAGTTTTACAGGGTGTTCCCTAATGGTGAGGTTCAGTATTTGCACCCAAAAGATGGAGTGTACCCTGAGAAGGTGAACCCAGGGCGCCAAGGAGTGGGGGTGAACTTCAGGTCCATTGGGAAGAATGTGAACCCAATTGAGGTCAAGTTCACCGGGAAGCAAGTGTATGACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SET

Protein Analysis

205

Amino Acids

22.6

Weight (kDa)

9.47

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaD PF02531 73 - 203 1.5e-68 PsaD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 88
AccB1I GGYRCC 2 cut(s) 157, 525
AccB7I CCANNNNNTGG 3 cut(s) 335, 494, 535
AciI CCGC 5 cut(s) 39, 108, 197, 237, 259
AclWI GGATC 1 cut(s) 137
AcoI YGGCCR 2 cut(s) 3, 378
AcuI CTGAAG 2 cut(s) 162, 532
AcyI GRCGYC 1 cut(s) 526
AfaI GTAC 3 cut(s) 428, 440, 503
AfiI CCNNNNNNNGG 7 cut(s) 233, 335, 351, 494, 511, 535, 580
AgeI ACCGGT 1 cut(s) 245
AgsI TTSAA 1 cut(s) 373
AjnI CCWGG 3 cut(s) 293, 380, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AloI GAACNNNNNNTCC 2 cut(s) 536, 568
AluBI AGCT 3 cut(s) 137, 179, 376
AluI AGCT 3 cut(s) 137, 179, 376
AlwI GGATC 1 cut(s) 137
AlwNI CAGNNNCTG 1 cut(s) 140
AoxI GGCC 4 cut(s) 3, 171, 264, 378
ApeKI GCWGC 5 cut(s) 110, 134, 137, 240, 341
AsiGI ACCGGT 1 cut(s) 245
AspLEI GCGC 2 cut(s) 115, 528
AspS9I GGNCC 5 cut(s) 208, 265, 359, 420, 552
AsuC2I CCSGG 1 cut(s) 595
AsuHPI GGTGA 9 cut(s) 22, 58, 184, 283, 294, 479, 526, 553, 583
AvaII GGWCC 4 cut(s) 208, 359, 420, 552
BalI TGGCCA 2 cut(s) 5, 380
BanI GGYRCC 2 cut(s) 157, 525
BbvCI CCTCAGC 1 cut(s) 43
BbvI GCAGC 5 cut(s) 122, 124, 146, 252, 353
BccI CCATC 3 cut(s) 229, 353, 488
BciT130I CCWGG 3 cut(s) 295, 382, 522
BclI TGATCA 1 cut(s) 288
BcnI CCSGG 1 cut(s) 595
BfaI CTAG 1 cut(s) 411
BfoI RGCGCY 2 cut(s) 116, 529
BisI GCNGC 6 cut(s) 111, 135, 138, 238, 241, 342
BlsI GCNGC 6 cut(s) 112, 136, 139, 239, 242, 343
Bme1390I CCNGG 4 cut(s) 295, 382, 522, 595
Bme18I GGWCC 4 cut(s) 208, 359, 420, 552
BmgT120I GGNCC 5 cut(s) 208, 265, 359, 420, 552
BmiI GGNNCC 3 cut(s) 159, 210, 527
BmrFI CCNGG 4 cut(s) 295, 382, 522, 595
BmsI GCATC 1 cut(s) 315
BoxI GACNNNNGTC 1 cut(s) 418
Bpu10I CCTNAGC 1 cut(s) 43
BpuMI CCSGG 1 cut(s) 595
BsaAI YACGTR 1 cut(s) 286
BsaHI GRCGYC 1 cut(s) 526
BsaJI CCNNGG 8 cut(s) 6, 50, 153, 294, 306, 520, 521, 529
BsaWI WCCGGW 2 cut(s) 181, 245
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bsc4I CCNNNNNNNGG 7 cut(s) 233, 335, 351, 494, 511, 535, 580
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 3 cut(s) 210, 337, 442
BseBI CCWGG 3 cut(s) 295, 382, 522
BseDI CCNNGG 8 cut(s) 6, 50, 153, 294, 306, 520, 521, 529
BseGI GGATG 1 cut(s) 221
BseLI CCNNNNNNNGG 7 cut(s) 233, 335, 351, 494, 511, 535, 580
BseMII CTCAG 3 cut(s) 57, 407, 498
BseNI ACTGG 3 cut(s) 210, 337, 442
BseXI GCAGC 5 cut(s) 122, 124, 146, 252, 353
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 4 cut(s) 5, 173, 266, 380
BshNI GGYRCC 2 cut(s) 157, 525
BshTI ACCGGT 1 cut(s) 245
BsiSI CCGG 4 cut(s) 182, 200, 246, 594
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 7 cut(s) 233, 335, 351, 494, 511, 535, 580
BsmFI GGGAC 1 cut(s) 421
BsnI GGCC 4 cut(s) 5, 173, 266, 380
Bsp143I GATC 3 cut(s) 129, 288, 432
Bsp19I CCATGG 2 cut(s) 6, 50
BspACI CCGC 5 cut(s) 39, 108, 197, 237, 259
BspANI GGCC 4 cut(s) 5, 173, 266, 380
BspCNI CTCAG 3 cut(s) 56, 408, 499
BspHI TCATGA 2 cut(s) 78, 348
BspLI GGNNCC 3 cut(s) 159, 210, 527
BspPI GGATC 1 cut(s) 137
BspT107I GGYRCC 2 cut(s) 157, 525
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 3 cut(s) 210, 337, 442
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 8 cut(s) 6, 50, 153, 294, 306, 520, 521, 529
BssMI GATC 3 cut(s) 129, 288, 432
BssNI GRCGYC 1 cut(s) 526
BssT1I CCWWGG 4 cut(s) 6, 50, 306, 529
Bst2UI CCWGG 3 cut(s) 295, 382, 522
Bst4CI ACNGT 2 cut(s) 60, 154
Bst6I CTCTTC 3 cut(s) 32, 138, 270
BstACI GRCGYC 1 cut(s) 526
BstBAI YACGTR 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 3 cut(s) 43, 416, 507
BstDSI CCRYGG 3 cut(s) 6, 50, 153
BstF5I GGATG 1 cut(s) 221
BstH2I RGCGCY 2 cut(s) 116, 529
BstHHI GCGC 2 cut(s) 115, 528
BstKTI GATC 3 cut(s) 132, 291, 435
BstMBI GATC 3 cut(s) 129, 288, 432
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 3 cut(s) 295, 382, 522
BstPAI GACNNNNGTC 1 cut(s) 418
BstSCI CCNGG 4 cut(s) 293, 380, 520, 593
BstV1I GCAGC 5 cut(s) 122, 124, 146, 252, 353
BsuRI GGCC 4 cut(s) 5, 173, 266, 380
BtgI CCRYGG 3 cut(s) 6, 50, 153
BtsCI GGATG 1 cut(s) 221
BtsI GCAGTG 1 cut(s) 398
BtsIMutI CAGTG 2 cut(s) 92, 398
Cac8I GCNNGC 1 cut(s) 378
CaiI CAGNNNCTG 1 cut(s) 140
CciI TCATGA 2 cut(s) 78, 348
CfoI GCGC 2 cut(s) 115, 528
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 5 cut(s) 208, 265, 359, 420, 552
Csp6I GTAC 3 cut(s) 427, 439, 502
CspAI ACCGGT 1 cut(s) 245
CviAII CATG 5 cut(s) 7, 51, 79, 124, 349
CviQI GTAC 3 cut(s) 427, 439, 502
DdeI CTNAG 3 cut(s) 43, 416, 507
DinI GGCGCC 1 cut(s) 527
DpnI GATC 3 cut(s) 131, 290, 434
DpnII GATC 3 cut(s) 129, 288, 432
DrdI GACNNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 2 cut(s) 3, 378
Eam1104I CTCTTC 3 cut(s) 32, 138, 270
EarI CTCTTC 3 cut(s) 32, 138, 270
Eco130I CCWWGG 4 cut(s) 6, 50, 306, 529
Eco47I GGWCC 4 cut(s) 208, 359, 420, 552
Eco57I CTGAAG 2 cut(s) 162, 532
EcoO109I RGGNCCY 1 cut(s) 359
EcoRII CCWGG 3 cut(s) 293, 380, 520
EcoT14I CCWWGG 4 cut(s) 6, 50, 306, 529
EgeI GGCGCC 1 cut(s) 527
EheI GGCGCC 1 cut(s) 527
ErhI CCWWGG 4 cut(s) 6, 50, 306, 529
FaeI CATG 5 cut(s) 10, 54, 82, 127, 352
FaiI YATR 8 cut(s) 8, 52, 80, 125, 350, 609, 614, 616
FaqI GGGAC 1 cut(s) 421
FatI CATG 5 cut(s) 6, 50, 78, 123, 348
FauI CCCGC 1 cut(s) 204
FauNDI CATATG 1 cut(s) 614
FbaI TGATCA 1 cut(s) 288
Fnu4HI GCNGC 6 cut(s) 111, 135, 138, 238, 241, 342
FokI GGATG 1 cut(s) 208
Fsp4HI GCNGC 6 cut(s) 111, 135, 138, 238, 241, 342
FspBI CTAG 1 cut(s) 411
GlaI GCGC 2 cut(s) 114, 527
GluI GCNGC 6 cut(s) 111, 135, 138, 238, 241, 342
HaeII RGCGCY 2 cut(s) 116, 529
HaeIII GGCC 4 cut(s) 5, 173, 266, 380
HapII CCGG 4 cut(s) 182, 200, 246, 594
HhaI GCGC 2 cut(s) 115, 528
Hin1I GRCGYC 1 cut(s) 526
Hin1II CATG 5 cut(s) 10, 54, 82, 127, 352
Hin6I GCGC 2 cut(s) 113, 526
HinP1I GCGC 2 cut(s) 113, 526
HinfI GANTC 1 cut(s) 299
HpaII CCGG 4 cut(s) 182, 200, 246, 594
HphI GGTGA 9 cut(s) 22, 58, 184, 283, 294, 479, 526, 553, 583
Hpy166II GTNNAC 6 cut(s) 105, 502, 517, 544, 571, 591
Hpy188III TCNNGA 2 cut(s) 79, 349
Hpy8I GTNNAC 6 cut(s) 105, 502, 517, 544, 571, 591
HpyAV CCTTC 3 cut(s) 85, 350, 505
HpyCH4III ACNGT 2 cut(s) 60, 154
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 2 cut(s) 341, 484
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 3 cut(s) 43, 416, 507
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 526
Hsp92II CATG 5 cut(s) 10, 54, 82, 127, 352
HspAI GCGC 2 cut(s) 113, 526
KasI GGCGCC 1 cut(s) 525
Ksp22I TGATCA 1 cut(s) 288
Kzo9I GATC 3 cut(s) 129, 288, 432
LmnI GCTCC 2 cut(s) 184, 310
Lsp1109I GCAGC 5 cut(s) 122, 124, 146, 252, 353
LweI GCATC 1 cut(s) 315
MaeI CTAG 1 cut(s) 411
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 2 cut(s) 64, 90
MalI GATC 3 cut(s) 131, 290, 434
MboI GATC 3 cut(s) 129, 288, 432
MboII GAAGA 5 cut(s) 19, 155, 223, 287, 574
MfeI CAATTG 1 cut(s) 576
MlsI TGGCCA 2 cut(s) 5, 380
MluCI AATT 1 cut(s) 576
MluNI TGGCCA 2 cut(s) 5, 380
Mly113I GGCGCC 1 cut(s) 526
Mox20I TGGCCA 2 cut(s) 5, 380
MscI TGGCCA 2 cut(s) 5, 380
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 2 cut(s) 5, 380
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 4 cut(s) 182, 200, 246, 594
MspR9I CCNGG 4 cut(s) 295, 382, 522, 595
MunI CAATTG 1 cut(s) 576
MvaI CCWGG 3 cut(s) 295, 382, 522
MwoI GCNNNNNNNGC 1 cut(s) 17
NarI GGCGCC 1 cut(s) 526
NciI CCSGG 1 cut(s) 595
NcoI CCATGG 2 cut(s) 6, 50
NdeI CATATG 1 cut(s) 614
NdeII GATC 3 cut(s) 129, 288, 432
NlaIII CATG 5 cut(s) 10, 54, 82, 127, 352
NlaIV GGNNCC 3 cut(s) 159, 210, 527
NmuCI GTSAC 2 cut(s) 64, 90
PagI TCATGA 2 cut(s) 78, 348
PasI CCCWGGG 1 cut(s) 521
PfeI GAWTC 1 cut(s) 299
PflFI GACNNNGTC 1 cut(s) 100
PflMI CCANNNNNTGG 3 cut(s) 335, 494, 535
PinAI ACCGGT 1 cut(s) 245
PkrI GCNGC 6 cut(s) 112, 136, 139, 239, 242, 343
PluTI GGCGCC 1 cut(s) 529
Ppu21I YACGTR 1 cut(s) 286
PpuMI RGGWCCY 1 cut(s) 359
PshAI GACNNNNGTC 1 cut(s) 418
Psp5II RGGWCCY 1 cut(s) 359
Psp6I CCWGG 3 cut(s) 293, 380, 520
PspGI CCWGG 3 cut(s) 293, 380, 520
PspN4I GGNNCC 3 cut(s) 159, 210, 527
PspPI GGNCC 5 cut(s) 208, 265, 359, 420, 552
PspPPI RGGWCCY 1 cut(s) 359
PstNI CAGNNNCTG 1 cut(s) 140
PsyI GACNNNGTC 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 3 cut(s) 428, 440, 503
RsaNI GTAC 3 cut(s) 427, 439, 502
RseI CAYNNNNRTG 1 cut(s) 77
SatI GCNGC 6 cut(s) 111, 135, 138, 238, 241, 342
Sau3AI GATC 3 cut(s) 129, 288, 432
Sau96I GGNCC 5 cut(s) 208, 265, 359, 420, 552
ScrFI CCNGG 4 cut(s) 295, 382, 522, 595
SfaNI GCATC 1 cut(s) 315
SfoI GGCGCC 1 cut(s) 527
SgrAI CRCCGGYG 1 cut(s) 245
SinI GGWCC 4 cut(s) 208, 359, 420, 552
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 15
SmoI CTYRAG 1 cut(s) 15
Sse9I AATT 1 cut(s) 576
SsiI CCGC 5 cut(s) 39, 108, 197, 237, 259
SspDI GGCGCC 1 cut(s) 525
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 4 cut(s) 293, 380, 520, 593
StyI CCWWGG 4 cut(s) 6, 50, 306, 529
TaaI ACNGT 2 cut(s) 60, 154
TaiI ACGT 1 cut(s) 288
TasI AATT 1 cut(s) 576
TatI WGTACW 1 cut(s) 426
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 1 cut(s) 299
TscAI CASTG 2 cut(s) 99, 398
TseFI GTSAC 2 cut(s) 64, 90
TseI GCWGC 5 cut(s) 110, 134, 137, 240, 341
Tsp45I GTSAC 2 cut(s) 64, 90
TspDTI ATGAA 1 cut(s) 67
TspRI CASTG 2 cut(s) 99, 398
Tth111I GACNNNGTC 1 cut(s) 100
Van91I CCANNNNNTGG 3 cut(s) 335, 494, 535
VpaK11BI GGWCC 4 cut(s) 208, 359, 420, 552
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.