Rw4G024410

Histone-lysine N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002127
Physical Location & Seq
Forward (+)
50220301 .. 50223528
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024410.1

Sequence Viewer

Length: 1191 bp
ATGGCAATGACAAAGGAGGTGAGGCTAACGGAGATACAAGGCAGAGGAAGGTCATTAGTGGCGGCTCAGCCACTCAAAGGCGGCCAAATCGTTCTCCGCGACTCTCCTCTCCTCCTCTACTCTGCTCTTCCTCTCCTCACTCCCTCTCCTCCCCCTTATTGCGACCACTGCTTCAAAACCCTAAACCCACAAACCGCCCCCATTTCACTCTGCCAATCCTGCTCCCTCCACGCCTTCTGCACCCCCAATTGCCTCTCCGCCGCCCAGTCCTCCTCCCACACTCAATGGGTCTGCCAATCCCTCACGAAATTACGAAATTGCCACTCCCCGCTCTCCGGCCAGCCCGCCGACCGTCAGGTCCAAGCTCGCTTCTTGATCGCCGCTTACAACCTCGCCGTCACTTCGCCGTCGAGTTTCCAGGTGCTGCTCTCTCTTCAGGGCCAGCCGCAGGACTCCGCCGACGCTCAGTTCCTGCATTCTCTGATTTCGACCCTCTGCCCTCCGCCGCAGCAGCAACTGTTTTCGGTGGAGCTCACGGCGGCGCTGTTGGCCAAGGACAAGATGAATGCGTTTGGACTGATGGAGCCCCCTTCGGAATTTGGGCTGAGATCGAGCAGAGCCTATGGAATTTATCCCAAGGCCTCGTTTTTCAACCATGATTGTCTCCCCAATGCGTGCCGGTTTGATTATGTTGATGAAGGCAGCACGGAGATTATTGTGAGGATGATTCACGATGTTCCGGAGGGGAGGGAGATTTGCCTGAGCTACTTTCCTGTTAACCAGGCTTATTCGAGCAGGCAGAGGACTCTGGCTGAGGACTATGGGTTTGTGTGTCAATGTGATCGATGCAAGGTTGAGGCGAATTGGTCTGACAATGAGGAGGTTGAGGAGGAAGGTGGTTCGGCTGAGGTTATGGATGAGGACCAAGACCAGGAAATGGAAGGAGAATCGGAATCAGAAGCTGGGAGTGACATTGAAGTAGAAGCTCAAGGAGAAGCTGATTTTCCACATGCCTATTTCTTTGTGAGATTTATGTGTAGCAGAACCAATTGCTGGGGCACGCTGGCGCCTTTGCCCCCAAAAGACGATGGAACGCCTTCGGATGCTATGGAATGCAATGTGTGTGGAAGTGTTAGGAAAGATGAGGGGATTAGTGGTCATGGACAAAATGGGCTCTCTTTGGATGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SET

Protein Analysis

396

Amino Acids

43.42

Weight (kDa)

4.7

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 206 - 256 5.8e-08 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 356
AccB1I GGYRCC 1 cut(s) 1066
AccB7I CCANNNNNTGG 2 cut(s) 937, 1053
AccBSI CCGCTC 1 cut(s) 331
AccII CGCG 1 cut(s) 99
AccIII TCCGGA 1 cut(s) 739
AcoI YGGCCR 3 cut(s) 82, 337, 549
AcsI RAATTY 2 cut(s) 596, 627
AcuI CTGAAG 1 cut(s) 419
AcyI GRCGYC 1 cut(s) 1067
AfiI CCNNNNNNNGG 6 cut(s) 77, 335, 448, 931, 937, 1053
AgsI TTSAA 3 cut(s) 175, 652, 977
AjnI CCWGG 3 cut(s) 417, 780, 930
AluBI AGCT 6 cut(s) 365, 532, 765, 962, 986, 998
AluI AGCT 6 cut(s) 365, 532, 765, 962, 986, 998
Alw21I GWGCWC 1 cut(s) 534
Alw26I GTCTC 1 cut(s) 668
AlwNI CAGNNNCTG 4 cut(s) 424, 472, 517, 962
Aor13HI TCCGGA 1 cut(s) 739
AoxI GGCC 5 cut(s) 82, 337, 439, 549, 639
ApeKI GCWGC 4 cut(s) 424, 508, 511, 702
ApoI RAATTY 2 cut(s) 596, 627
ArsI GACNNNNNNTTYG 2 cut(s) 809, 841
Asp700I GAANNNNTTC 1 cut(s) 1096
AspLEI GCGC 2 cut(s) 544, 1069
AspS9I GGNCC 3 cut(s) 358, 439, 922
AsuHPI GGTGA 1 cut(s) 31
AvaII GGWCC 2 cut(s) 358, 922
BaeGI GKGCMC 1 cut(s) 1061
BalI TGGCCA 1 cut(s) 551
BanI GGYRCC 1 cut(s) 1066
BanII GRGCYC 3 cut(s) 534, 588, 1176
Bbv12I GWGCWC 1 cut(s) 534
BbvCI CCTCAGC 2 cut(s) 813, 906
BbvI GCAGC 4 cut(s) 411, 520, 523, 714
BccI CCATC 3 cut(s) 574, 1082, 1178
BceAI ACGGC 3 cut(s) 380, 391, 552
BciT130I CCWGG 3 cut(s) 419, 782, 932
BcoDI GTCTC 1 cut(s) 668
BfoI RGCGCY 2 cut(s) 545, 1070
BlpI GCTNAGC 1 cut(s) 66
Bme1390I CCNGG 3 cut(s) 419, 782, 932
Bme18I GGWCC 2 cut(s) 358, 922
BmgT120I GGNCC 3 cut(s) 358, 439, 922
BmiI GGNNCC 2 cut(s) 585, 1068
BmrFI CCNGG 3 cut(s) 419, 782, 932
BmrI ACTGGG 1 cut(s) 259
BmsI GCATC 2 cut(s) 836, 1093
BmuI ACTGGG 1 cut(s) 259
Bpu10I CCTNAGC 3 cut(s) 761, 813, 906
Bpu1102I GCTNAGC 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 972
Bsa29I ATCGAT 1 cut(s) 844
BsaHI GRCGYC 1 cut(s) 1067
BsaJI CCNNGG 2 cut(s) 552, 636
BsaWI WCCGGW 1 cut(s) 739
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 6 cut(s) 77, 335, 448, 931, 937, 1053
Bse118I RCCGGY 1 cut(s) 678
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 2 cut(s) 12, 1123
BseAI TCCGGA 1 cut(s) 739
BseBI CCWGG 3 cut(s) 419, 782, 932
BseCI ATCGAT 1 cut(s) 844
BseDI CCNNGG 2 cut(s) 552, 636
BseGI GGATG 4 cut(s) 729, 922, 1108, 1189
BseLI CCNNNNNNNGG 6 cut(s) 77, 335, 448, 931, 937, 1053
BseMI GCAATG 2 cut(s) 12, 1123
BseMII CTCAG 6 cut(s) 80, 479, 596, 752, 804, 897
BseNI ACTGG 1 cut(s) 265
BseRI GAGGAG 8 cut(s) 96, 101, 104, 125, 138, 262, 893, 902
BseSI GKGCMC 1 cut(s) 1061
BseXI GCAGC 4 cut(s) 411, 520, 523, 714
BseYI CCCAGC 2 cut(s) 962, 1053
BsgI GTGCAG 1 cut(s) 223
Bsh1236I CGCG 1 cut(s) 99
Bsh1285I CGRYCG 1 cut(s) 352
BshFI GGCC 5 cut(s) 84, 339, 441, 551, 641
BshNI GGYRCC 1 cut(s) 1066
BshVI ATCGAT 1 cut(s) 844
BsiEI CGRYCG 1 cut(s) 352
BsiHKAI GWGCWC 1 cut(s) 534
BsiSI CCGG 3 cut(s) 336, 679, 740
BslI CCNNNNNNNGG 6 cut(s) 77, 335, 448, 931, 937, 1053
BsmAI GTCTC 1 cut(s) 668
BsmI GAATGC 3 cut(s) 475, 571, 1118
BsnI GGCC 5 cut(s) 84, 339, 441, 551, 641
Bsp1286I GDGCHC 4 cut(s) 534, 588, 1061, 1176
Bsp13I TCCGGA 1 cut(s) 739
Bsp143I GATC 3 cut(s) 375, 608, 841
Bsp1720I GCTNAGC 1 cut(s) 66
BspANI GGCC 5 cut(s) 84, 339, 441, 551, 641
BspCNI CTCAG 6 cut(s) 79, 478, 597, 753, 805, 898
BspDI ATCGAT 1 cut(s) 844
BspEI TCCGGA 1 cut(s) 739
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 2 cut(s) 585, 1068
BspQI GCTCTTC 1 cut(s) 132
BspT107I GGYRCC 1 cut(s) 1066
BsrBI CCGCTC 1 cut(s) 331
BsrDI GCAATG 2 cut(s) 12, 1123
BsrFI RCCGGY 1 cut(s) 678
BsrI ACTGG 1 cut(s) 265
BssAI RCCGGY 1 cut(s) 678
BssECI CCNNGG 2 cut(s) 552, 636
BssMI GATC 3 cut(s) 375, 608, 841
BssNI GRCGYC 1 cut(s) 1067
BssT1I CCWWGG 2 cut(s) 552, 636
Bst2UI CCWGG 3 cut(s) 419, 782, 932
Bst4CI ACNGT 2 cut(s) 353, 519
Bst6I CTCTTC 2 cut(s) 132, 438
BstACI GRCGYC 1 cut(s) 1067
BstC8I GCNNGC 8 cut(s) 341, 345, 367, 443, 676, 797, 1061, 1065
BstDEI CTNAG 6 cut(s) 66, 465, 605, 761, 813, 906
BstF5I GGATG 4 cut(s) 729, 922, 1108, 1189
BstFNI CGCG 1 cut(s) 99
BstH2I RGCGCY 2 cut(s) 545, 1070
BstHHI GCGC 2 cut(s) 544, 1069
BstKTI GATC 3 cut(s) 378, 611, 844
BstMAI GTCTC 1 cut(s) 668
BstMBI GATC 3 cut(s) 375, 608, 841
BstMCI CGRYCG 1 cut(s) 352
BstMWI GCNNNNNNNGC 4 cut(s) 168, 219, 511, 548
BstNI CCWGG 3 cut(s) 419, 782, 932
BstNSI RCATGY 1 cut(s) 1013
BstSCI CCNGG 3 cut(s) 417, 780, 930
BstSLI GKGCMC 1 cut(s) 1061
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 4 cut(s) 411, 520, 523, 714
Bsu15I ATCGAT 1 cut(s) 844
BsuRI GGCC 5 cut(s) 84, 339, 441, 551, 641
BsuTUI ATCGAT 1 cut(s) 844
BtsCI GGATG 4 cut(s) 729, 922, 1108, 1189
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 8 cut(s) 341, 345, 367, 443, 676, 797, 1061, 1065
CaiI CAGNNNCTG 4 cut(s) 424, 472, 517, 962
CfoI GCGC 2 cut(s) 544, 1069
Cfr10I RCCGGY 1 cut(s) 678
Cfr13I GGNCC 3 cut(s) 358, 439, 922
ClaI ATCGAT 1 cut(s) 844
CseI GACGC 1 cut(s) 470
CspCI CAANNNNNGTGG 2 cut(s) 1105, 1140
CviAII CATG 3 cut(s) 656, 1010, 1160
DdeI CTNAG 6 cut(s) 66, 465, 605, 761, 813, 906
DinI GGCGCC 1 cut(s) 1068
DpnI GATC 3 cut(s) 377, 610, 843
DpnII GATC 3 cut(s) 375, 608, 841
DrdI GACNNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 356
EaeI YGGCCR 3 cut(s) 82, 337, 549
Eam1104I CTCTTC 2 cut(s) 132, 438
EarI CTCTTC 2 cut(s) 132, 438
EciI GGCGGA 3 cut(s) 247, 445, 492
Ecl136II GAGCTC 1 cut(s) 532
Eco130I CCWWGG 2 cut(s) 552, 636
Eco147I AGGCCT 1 cut(s) 641
Eco24I GRGCYC 3 cut(s) 534, 588, 1176
Eco47I GGWCC 2 cut(s) 358, 922
Eco53kI GAGCTC 1 cut(s) 532
Eco57I CTGAAG 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 532
EcoRII CCWGG 3 cut(s) 417, 780, 930
EcoT14I CCWWGG 2 cut(s) 552, 636
EcoT38I GRGCYC 3 cut(s) 534, 588, 1176
EgeI GGCGCC 1 cut(s) 1068
EheI GGCGCC 1 cut(s) 1068
ErhI CCWWGG 2 cut(s) 552, 636
FaeI CATG 3 cut(s) 659, 1013, 1163
FaiI YATR 9 cut(s) 624, 657, 690, 822, 914, 1011, 1034, 1109, 1161
FatI CATG 3 cut(s) 655, 1009, 1159
FauI CCCGC 2 cut(s) 336, 352
FokI GGATG 3 cut(s) 736, 929, 1115
FriOI GRGCYC 3 cut(s) 534, 588, 1176
GlaI GCGC 2 cut(s) 543, 1068
GsaI CCCAGC 2 cut(s) 966, 1057
HaeII RGCGCY 2 cut(s) 545, 1070
HaeIII GGCC 5 cut(s) 84, 339, 441, 551, 641
HapII CCGG 3 cut(s) 336, 679, 740
HgaI GACGC 1 cut(s) 470
HhaI GCGC 2 cut(s) 544, 1069
Hin1I GRCGYC 1 cut(s) 1067
Hin1II CATG 3 cut(s) 659, 1013, 1163
Hin6I GCGC 2 cut(s) 542, 1067
HinP1I GCGC 2 cut(s) 542, 1067
HincII GTYRAC 1 cut(s) 778
HindII GTYRAC 1 cut(s) 778
HinfI GANTC 6 cut(s) 101, 452, 727, 805, 947, 953
HpaI GTTAAC 1 cut(s) 778
HpaII CCGG 3 cut(s) 336, 679, 740
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 1 cut(s) 778
Hpy188I TCNGA 6 cut(s) 483, 595, 871, 952, 958, 1102
Hpy188III TCNNGA 4 cut(s) 304, 373, 731, 740
Hpy8I GTNNAC 1 cut(s) 778
Hpy99I CGWCG 2 cut(s) 412, 464
HpyAV CCTTC 7 cut(s) 42, 244, 600, 692, 887, 935, 1107
HpyCH4III ACNGT 2 cut(s) 353, 519
HpyCH4V TGCA 4 cut(s) 240, 475, 849, 1116
HpyF10VI GCNNNNNNNGC 4 cut(s) 168, 219, 511, 548
HpyF3I CTNAG 6 cut(s) 66, 465, 605, 761, 813, 906
Hsp92I GRCGYC 1 cut(s) 1067
Hsp92II CATG 3 cut(s) 659, 1013, 1163
HspAI GCGC 2 cut(s) 542, 1067
KasI GGCGCC 1 cut(s) 1066
Kpn2I TCCGGA 1 cut(s) 739
KspAI GTTAAC 1 cut(s) 778
Kzo9I GATC 3 cut(s) 375, 608, 841
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 3 cut(s) 227, 529, 583
Lsp1109I GCAGC 4 cut(s) 411, 520, 523, 714
LweI GCATC 2 cut(s) 836, 1093
MaeIII GTNAC 2 cut(s) 397, 968
MalI GATC 3 cut(s) 377, 610, 843
MbiI CCGCTC 1 cut(s) 331
MboI GATC 3 cut(s) 375, 608, 841
MboII GAAGA 2 cut(s) 119, 425
MfeI CAATTG 2 cut(s) 247, 1048
MhlI GDGCHC 4 cut(s) 534, 588, 1061, 1176
MlsI TGGCCA 1 cut(s) 551
MluCI AATT 7 cut(s) 247, 308, 316, 596, 627, 862, 1048
MluNI TGGCCA 1 cut(s) 551
Mly113I GGCGCC 1 cut(s) 1067
MlyI GAGTC 3 cut(s) 95, 446, 799
Mox20I TGGCCA 1 cut(s) 551
MroI TCCGGA 1 cut(s) 739
MroXI GAANNNNTTC 1 cut(s) 1096
MscI TGGCCA 1 cut(s) 551
MseI TTAA 1 cut(s) 777
Msp20I TGGCCA 1 cut(s) 551
MspI CCGG 3 cut(s) 336, 679, 740
MspR9I CCNGG 3 cut(s) 419, 782, 932
MunI CAATTG 2 cut(s) 247, 1048
Mva1269I GAATGC 3 cut(s) 475, 571, 1118
MvaI CCWGG 3 cut(s) 419, 782, 932
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 4 cut(s) 168, 219, 511, 548
NarI GGCGCC 1 cut(s) 1067
NdeII GATC 3 cut(s) 375, 608, 841
NlaIII CATG 3 cut(s) 659, 1013, 1163
NlaIV GGNNCC 2 cut(s) 585, 1068
NmuCI GTSAC 2 cut(s) 397, 968
NspI RCATGY 1 cut(s) 1013
PceI AGGCCT 1 cut(s) 641
PciSI GCTCTTC 1 cut(s) 132
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 3 cut(s) 475, 571, 1118
PdmI GAANNNNTTC 1 cut(s) 1096
PfeI GAWTC 3 cut(s) 727, 947, 953
PflMI CCANNNNNTGG 2 cut(s) 937, 1053
PleI GAGTC 3 cut(s) 95, 446, 799
PluTI GGCGCC 1 cut(s) 1070
PpsI GAGTC 3 cut(s) 95, 446, 799
Psp124BI GAGCTC 1 cut(s) 534
Psp6I CCWGG 3 cut(s) 417, 780, 930
PspFI CCCAGC 2 cut(s) 962, 1053
PspGI CCWGG 3 cut(s) 417, 780, 930
PspN4I GGNNCC 2 cut(s) 585, 1068
PspPI GGNCC 3 cut(s) 358, 439, 922
PstNI CAGNNNCTG 4 cut(s) 424, 472, 517, 962
SacI GAGCTC 1 cut(s) 534
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 1 cut(s) 777
Sau3AI GATC 3 cut(s) 375, 608, 841
Sau96I GGNCC 3 cut(s) 358, 439, 922
SchI GAGTC 3 cut(s) 95, 446, 799
ScrFI CCNGG 3 cut(s) 419, 782, 932
SduI GDGCHC 4 cut(s) 534, 588, 1061, 1176
SfaNI GCATC 2 cut(s) 836, 1093
SfoI GGCGCC 1 cut(s) 1068
SinI GGWCC 2 cut(s) 358, 922
SmlI CTYRAG 1 cut(s) 987
SmoI CTYRAG 1 cut(s) 987
Sse9I AATT 7 cut(s) 247, 308, 316, 596, 627, 862, 1048
SseBI AGGCCT 1 cut(s) 641
SspDI GGCGCC 1 cut(s) 1066
SstI GAGCTC 1 cut(s) 534
StuI AGGCCT 1 cut(s) 641
StyD4I CCNGG 3 cut(s) 417, 780, 930
StyI CCWWGG 2 cut(s) 552, 636
TaaI ACNGT 2 cut(s) 353, 519
TaqI TCGA 5 cut(s) 410, 488, 611, 791, 844
TasI AATT 7 cut(s) 247, 308, 316, 596, 627, 862, 1048
TauI GCSGC 7 cut(s) 65, 84, 263, 383, 448, 508, 542
TfiI GAWTC 3 cut(s) 727, 947, 953
Tru1I TTAA 1 cut(s) 777
Tru9I TTAA 1 cut(s) 777
TscAI CASTG 1 cut(s) 173
TseFI GTSAC 2 cut(s) 397, 968
TseI GCWGC 4 cut(s) 424, 508, 511, 702
Tsp45I GTSAC 2 cut(s) 397, 968
TspDTI ATGAA 2 cut(s) 578, 711
TspGWI ACGGA 2 cut(s) 44, 722
TspRI CASTG 1 cut(s) 173
Van91I CCANNNNNTGG 2 cut(s) 937, 1053
VpaK11BI GGWCC 2 cut(s) 358, 922
XapI RAATTY 2 cut(s) 596, 627
XceI RCATGY 1 cut(s) 1013
XmnI GAANNNNTTC 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.