MD16G1130700.v1.1

Histone-lysine N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002127
Physical Location & Seq
Reverse (-)
9999424 .. 10002951
3528 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1130700.v1.1.491

Sequence Viewer

Length: 1242 bp
ATGACGAAGGAATCATTGAGGGTAACGGAGATAGAAGGGAGAGGACGGGGGTTAGTGGCTGCTCAGCCACTCAGGGGCGGCGAAATAGTTCTCAGAGACTCTCCGATCCTCCTCTACTCCGCTCTTTCTCTCGTTGCTCCTTCTCCCCAACCTTACTGCCAACACTGCTTCAAAACCTTACAAAAACCCCAACCTCAAGCTCAACCCCAAGTATGTCCATTGTGTTCGCACCATGTCTTCTGCGGCCCGAATTGCCTCTCCACCGCTCTCTCATCCTCTCACTCCCCTTGGGCCTGCTATGCCCTCCGCCTTCTCCGTGACTCCCCTTCGCCGCTCTCCGGCCAGCCGCTCGAGCGGCAGGTCCAGGCTAGTTTCCTGATCGCCGCCTACAACCTCGCGGCGGTTTCCCCTTCCGATTTCCAGATTCTATTGTCTCTCCAGGGCCAGCCACACGACCTCGCCGACGCCGACACCACGGCTGCTGCCCAGTTTCTTCACTCTCTCATCTCGACACTCTGTGCTCCCCACCCAGTGTTCCTAGAGCAGCCGTTTACGGTGGAGCTCTCGGCGGCCCTGCTGGCCAAGGACAAGCTCAACGCCTTCGGATTGATGGAACCCTTCACGGAATTGTCGCAGAGAGCGAGCAGGGCCTACGCCATTTATCCGAAAGCTTCGTTCTTTAACCACGACTGCCTCCCCAATGCCTGCCGCTTCGATTATATCGACACAGACAGCGAACACAACACCGACATGGTTATCAGGATGATCCATGATGTTCCAGCGGGGAGGGAGATTTGCCTGAGCTATTTTCCGGTTAACCAGAGCTATTCGAACCGGCAGAGGACTCTGGCGGAGGACTATGGCTTCGCTTGCCAATGTGATCGCTGCAAGGTGGAAACTAATTGGTCTGACAATGAGGGTGAGGAAGAAGAAGAGTGTATGGATGAGGACCAAGACCAGGAAATGGAAGCAGCCTTGGAATCAGAACTGGAATCCGGAAATAACATTGAAGAAGCCGAAGCTCAAGCCCAACGGGAAGCCGGGAATAACGTCGAAGAAGCCGAAGCTCAAGCCCAAGGGGAATCCGATTTCCCACACGCCTATTTCTTTCTCAGGTTCATGTGCAATAGAACCAATTGCTGGGGCACTCTTGCTCCATTGCCCCCAAAAGATGACGGAACTTCTTCGAATGTCATGGAGTGCAATGTCTGTGGCAACCTTCAGAAAGATGAGGATAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SET

Protein Analysis

414

Amino Acids

45.76

Weight (kDa)

4.58

Isoelectric Point (pI)

60.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 15 - 269 7.3e-09 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 349
AccB7I CCANNNNNTGG 2 cut(s) 964, 1140
AccBSI CCGCTC 5 cut(s) 122, 266, 334, 349, 355
AccII CGCG 1 cut(s) 398
AccIII TCCGGA 1 cut(s) 995
AclWI GGATC 2 cut(s) 100, 760
AcoI YGGCCR 2 cut(s) 340, 579
AcuI CTGAAG 1 cut(s) 1205
AcyI GRCGYC 1 cut(s) 465
AdeI CACNNNGTG 2 cut(s) 518, 532
AfiI CCNNNNNNNGG 6 cut(s) 74, 338, 400, 958, 964, 1140
AgsI TTSAA 2 cut(s) 172, 1010
AjnI CCWGG 3 cut(s) 363, 438, 957
AluBI AGCT 9 cut(s) 200, 562, 592, 671, 804, 825, 1022, 1067, 1239
AluI AGCT 9 cut(s) 200, 562, 592, 671, 804, 825, 1022, 1067, 1239
Alw21I GWGCWC 2 cut(s) 523, 564
Alw26I GTCTC 2 cut(s) 90, 438
AlwI GGATC 2 cut(s) 100, 760
Ama87I CYCGRG 1 cut(s) 350
Aor13HI TCCGGA 1 cut(s) 995
AoxI GGCC 7 cut(s) 244, 291, 340, 442, 570, 579, 648
ApeKI GCWGC 6 cut(s) 59, 479, 482, 544, 885, 971
ArsI GACNNNNNNTTYG 2 cut(s) 848, 880
Asp700I GAANNNNTTC 2 cut(s) 87, 1183
AspS9I GGNCC 7 cut(s) 245, 291, 361, 442, 571, 648, 949
AsuC2I CCSGG 1 cut(s) 1042
AsuHPI GGTGA 1 cut(s) 932
AsuII TTCGAA 2 cut(s) 830, 1187
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 2 cut(s) 361, 949
BaeGI GKGCMC 1 cut(s) 1148
BalI TGGCCA 1 cut(s) 581
BanII GRGCYC 1 cut(s) 564
BbsI GAAGAC 1 cut(s) 229
Bbv12I GWGCWC 2 cut(s) 523, 564
BbvI GCAGC 6 cut(s) 46, 466, 469, 556, 872, 983
BccI CCATC 1 cut(s) 604
BceAI ACGGC 2 cut(s) 492, 532
BciT130I CCWGG 3 cut(s) 365, 440, 959
BcnI CCSGG 1 cut(s) 1042
BcoDI GTCTC 2 cut(s) 90, 438
BfaI CTAG 2 cut(s) 369, 539
BfuAI ACCTGC 1 cut(s) 349
BglI GCCNNNNNGGC 1 cut(s) 578
BlpI GCTNAGC 1 cut(s) 63
Bme1390I CCNGG 4 cut(s) 365, 440, 959, 1042
Bme18I GGWCC 2 cut(s) 361, 949
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 7 cut(s) 245, 291, 361, 442, 571, 648, 949
BmiI GGNNCC 1 cut(s) 615
BmrFI CCNGG 4 cut(s) 365, 440, 959, 1042
BmrI ACTGGG 2 cut(s) 481, 524
BmuI ACTGGG 2 cut(s) 481, 524
BpiI GAAGAC 1 cut(s) 229
BpmI CTGGAG 1 cut(s) 422
Bpu10I CCTNAGC 1 cut(s) 800
Bpu1102I GCTNAGC 1 cut(s) 63
Bpu14I TTCGAA 2 cut(s) 830, 1187
BpuEI CTTGAG 3 cut(s) 180, 1008, 1053
BpuMI CCSGG 1 cut(s) 1042
BsaHI GRCGYC 1 cut(s) 465
BsaJI CCNNGG 6 cut(s) 287, 439, 474, 582, 975, 1075
BsaWI WCCGGW 2 cut(s) 811, 995
BsaXI ACNNNNNCTCC 2 cut(s) 1138, 1168
Bsc4I CCNNNNNNNGG 6 cut(s) 74, 338, 400, 958, 964, 1140
Bse118I RCCGGY 1 cut(s) 834
Bse1I ACTGG 3 cut(s) 487, 530, 993
Bse3DI GCAATG 2 cut(s) 1157, 1210
BseAI TCCGGA 1 cut(s) 995
BseBI CCWGG 3 cut(s) 365, 440, 959
BseDI CCNNGG 6 cut(s) 287, 439, 474, 582, 975, 1075
BseGI GGATG 3 cut(s) 272, 768, 949
BseLI CCNNNNNNNGG 6 cut(s) 74, 338, 400, 958, 964, 1140
BseMI GCAATG 2 cut(s) 1157, 1210
BseMII CTCAG 5 cut(s) 77, 85, 106, 791, 1126
BseNI ACTGG 3 cut(s) 487, 530, 993
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 1 cut(s) 1148
BseXI GCAGC 6 cut(s) 46, 466, 469, 556, 872, 983
BseYI CCCAGC 1 cut(s) 1140
Bsh1236I CGCG 1 cut(s) 398
BshFI GGCC 7 cut(s) 246, 293, 342, 444, 572, 581, 650
BsiHKAI GWGCWC 2 cut(s) 523, 564
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 5 cut(s) 339, 812, 835, 996, 1041
BslI CCNNNNNNNGG 6 cut(s) 74, 338, 400, 958, 964, 1140
BsmAI GTCTC 2 cut(s) 90, 438
BsnI GGCC 7 cut(s) 246, 293, 342, 444, 572, 581, 650
BsoBI CYCGRG 1 cut(s) 350
Bsp119I TTCGAA 2 cut(s) 830, 1187
Bsp1286I GDGCHC 3 cut(s) 523, 564, 1148
Bsp13I TCCGGA 1 cut(s) 995
Bsp143I GATC 4 cut(s) 105, 378, 765, 880
Bsp1720I GCTNAGC 1 cut(s) 63
BspANI GGCC 7 cut(s) 246, 293, 342, 444, 572, 581, 650
BspCNI CTCAG 5 cut(s) 76, 84, 105, 792, 1125
BspEI TCCGGA 1 cut(s) 995
BspFNI CGCG 1 cut(s) 398
BspLI GGNNCC 1 cut(s) 615
BspMI ACCTGC 1 cut(s) 349
BspPI GGATC 2 cut(s) 100, 760
BspT104I TTCGAA 2 cut(s) 830, 1187
BsrBI CCGCTC 5 cut(s) 122, 266, 334, 349, 355
BsrDI GCAATG 2 cut(s) 1157, 1210
BsrFI RCCGGY 1 cut(s) 834
BsrI ACTGG 3 cut(s) 487, 530, 993
BssAI RCCGGY 1 cut(s) 834
BssECI CCNNGG 6 cut(s) 287, 439, 474, 582, 975, 1075
BssMI GATC 4 cut(s) 105, 378, 765, 880
BssNI GRCGYC 1 cut(s) 465
BssT1I CCWWGG 4 cut(s) 287, 582, 975, 1075
Bst2UI CCWGG 3 cut(s) 365, 440, 959
Bst4CI ACNGT 1 cut(s) 556
Bst6I CTCTTC 1 cut(s) 927
BstACI GRCGYC 1 cut(s) 465
BstBI TTCGAA 2 cut(s) 830, 1187
BstC8I GCNNGC 7 cut(s) 295, 344, 446, 579, 643, 706, 871
BstDEI CTNAG 5 cut(s) 63, 71, 92, 800, 1112
BstDSI CCRYGG 1 cut(s) 474
BstF5I GGATG 3 cut(s) 272, 768, 949
BstFNI CGCG 1 cut(s) 398
BstKTI GATC 4 cut(s) 108, 381, 768, 883
BstMAI GTCTC 2 cut(s) 90, 438
BstMBI GATC 4 cut(s) 105, 378, 765, 880
BstMWI GCNNNNNNNGC 8 cut(s) 165, 252, 299, 352, 355, 578, 647, 870
BstNI CCWGG 3 cut(s) 365, 440, 959
BstSCI CCNGG 4 cut(s) 363, 438, 957, 1040
BstSLI GKGCMC 1 cut(s) 1148
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 6 cut(s) 46, 466, 469, 556, 872, 983
BstV2I GAAGAC 1 cut(s) 229
BsuRI GGCC 7 cut(s) 246, 293, 342, 444, 572, 581, 650
BtgI CCRYGG 1 cut(s) 474
BtsCI GGATG 3 cut(s) 272, 768, 949
BtsI GCAGTG 1 cut(s) 163
BtsIMutI CAGTG 2 cut(s) 163, 537
BveI ACCTGC 1 cut(s) 349
Cac8I GCNNGC 7 cut(s) 295, 344, 446, 579, 643, 706, 871
Cfr10I RCCGGY 1 cut(s) 834
Cfr13I GGNCC 7 cut(s) 245, 291, 361, 442, 571, 648, 949
CseI GACGC 1 cut(s) 473
CspCI CAANNNNNGTGG 2 cut(s) 1192, 1227
CviAII CATG 5 cut(s) 233, 751, 770, 1120, 1195
DdeI CTNAG 5 cut(s) 63, 71, 92, 800, 1112
DpnI GATC 4 cut(s) 107, 380, 767, 882
DpnII GATC 4 cut(s) 105, 378, 765, 880
DraIII CACNNNGTG 2 cut(s) 518, 532
EaeI YGGCCR 2 cut(s) 340, 579
Eam1104I CTCTTC 1 cut(s) 927
EarI CTCTTC 1 cut(s) 927
EciI GGCGGA 2 cut(s) 296, 866
Ecl136II GAGCTC 1 cut(s) 562
Eco130I CCWWGG 4 cut(s) 287, 582, 975, 1075
Eco24I GRGCYC 1 cut(s) 564
Eco47I GGWCC 2 cut(s) 361, 949
Eco53kI GAGCTC 1 cut(s) 562
Eco57I CTGAAG 1 cut(s) 1205
Eco88I CYCGRG 1 cut(s) 350
EcoICRI GAGCTC 1 cut(s) 562
EcoO109I RGGNCCY 1 cut(s) 648
EcoRII CCWGG 3 cut(s) 363, 438, 957
EcoT14I CCWWGG 4 cut(s) 287, 582, 975, 1075
EcoT38I GRGCYC 1 cut(s) 564
ErhI CCWWGG 4 cut(s) 287, 582, 975, 1075
FaeI CATG 5 cut(s) 236, 754, 773, 1123, 1198
FatI CATG 5 cut(s) 232, 750, 769, 1119, 1194
FauI CCCGC 1 cut(s) 775
FokI GGATG 3 cut(s) 259, 775, 956
FriOI GRGCYC 1 cut(s) 564
FspBI CTAG 2 cut(s) 369, 539
GsaI CCCAGC 1 cut(s) 1144
GsuI CTGGAG 1 cut(s) 422
HaeIII GGCC 7 cut(s) 246, 293, 342, 444, 572, 581, 650
HapII CCGG 5 cut(s) 339, 812, 835, 996, 1041
HgaI GACGC 1 cut(s) 473
Hin1I GRCGYC 1 cut(s) 465
Hin1II CATG 5 cut(s) 236, 754, 773, 1123, 1198
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HindIII AAGCTT 1 cut(s) 669
HinfI GANTC 8 cut(s) 11, 98, 320, 424, 844, 980, 992, 1082
HpaI GTTAAC 1 cut(s) 817
HpaII CCGG 5 cut(s) 339, 812, 835, 996, 1041
HphI GGTGA 1 cut(s) 932
Hpy166II GTNNAC 2 cut(s) 552, 817
Hpy188I TCNGA 9 cut(s) 95, 105, 415, 605, 666, 910, 985, 1087, 1224
Hpy188III TCNNGA 5 cut(s) 376, 421, 508, 760, 996
Hpy8I GTNNAC 2 cut(s) 552, 817
Hpy99I CGWCG 2 cut(s) 467, 1055
HpyAV CCTTC 8 cut(s) 29, 150, 320, 336, 420, 610, 628, 1229
HpyCH4III ACNGT 1 cut(s) 556
HpyCH4IV ACGT 1 cut(s) 1050
HpyCH4V TGCA 3 cut(s) 888, 1125, 1203
HpyF10VI GCNNNNNNNGC 8 cut(s) 165, 252, 299, 352, 355, 578, 647, 870
HpyF3I CTNAG 5 cut(s) 63, 71, 92, 800, 1112
HpySE526I ACGT 1 cut(s) 1050
Hsp92I GRCGYC 1 cut(s) 465
Hsp92II CATG 5 cut(s) 236, 754, 773, 1123, 1198
Kpn2I TCCGGA 1 cut(s) 995
KspAI GTTAAC 1 cut(s) 817
Kzo9I GATC 4 cut(s) 105, 378, 765, 880
LmnI GCTCC 4 cut(s) 142, 526, 559, 1159
Lsp1109I GCAGC 6 cut(s) 46, 466, 469, 556, 872, 983
MaeI CTAG 2 cut(s) 369, 539
MaeII ACGT 1 cut(s) 1050
MaeIII GTNAC 2 cut(s) 22, 317
MalI GATC 4 cut(s) 107, 380, 767, 882
MbiI CCGCTC 5 cut(s) 122, 266, 334, 349, 355
MboI GATC 4 cut(s) 105, 378, 765, 880
MboII GAAGA 8 cut(s) 229, 485, 938, 941, 944, 1022, 1067, 1176
MfeI CAATTG 1 cut(s) 1135
MhlI GDGCHC 3 cut(s) 523, 564, 1148
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 4 cut(s) 250, 626, 901, 1135
MluNI TGGCCA 1 cut(s) 581
MlyI GAGTC 3 cut(s) 92, 314, 838
Mox20I TGGCCA 1 cut(s) 581
MroI TCCGGA 1 cut(s) 995
MroXI GAANNNNTTC 2 cut(s) 87, 1183
MscI TGGCCA 1 cut(s) 581
MseI TTAA 2 cut(s) 681, 816
MslI CAYNNNNRTG 1 cut(s) 749
Msp20I TGGCCA 1 cut(s) 581
MspA1I CMGCKG 1 cut(s) 782
MspI CCGG 5 cut(s) 339, 812, 835, 996, 1041
MspR9I CCNGG 4 cut(s) 365, 440, 959, 1042
MunI CAATTG 1 cut(s) 1135
MvaI CCWGG 3 cut(s) 365, 440, 959
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 8 cut(s) 165, 252, 299, 352, 355, 578, 647, 870
NciI CCSGG 1 cut(s) 1042
NdeII GATC 4 cut(s) 105, 378, 765, 880
NlaIII CATG 5 cut(s) 236, 754, 773, 1123, 1198
NlaIV GGNNCC 1 cut(s) 615
NmeAIII GCCGAG 1 cut(s) 545
NmuCI GTSAC 1 cut(s) 317
NspV TTCGAA 2 cut(s) 830, 1187
PaeR7I CTCGAG 1 cut(s) 350
PcsI WCGNNNNNNNCGW 5 cut(s) 459, 465, 638, 720, 1059
PdmI GAANNNNTTC 2 cut(s) 87, 1183
PfeI GAWTC 5 cut(s) 11, 424, 980, 992, 1082
PflMI CCANNNNNTGG 2 cut(s) 964, 1140
PleI GAGTC 3 cut(s) 92, 314, 838
PpsI GAGTC 3 cut(s) 92, 314, 838
Psp124BI GAGCTC 1 cut(s) 564
Psp6I CCWGG 3 cut(s) 363, 438, 957
PspFI CCCAGC 1 cut(s) 1140
PspGI CCWGG 3 cut(s) 363, 438, 957
PspN4I GGNNCC 1 cut(s) 615
PspPI GGNCC 7 cut(s) 245, 291, 361, 442, 571, 648, 949
PspXI VCTCGAGB 1 cut(s) 350
RseI CAYNNNNRTG 1 cut(s) 749
SacI GAGCTC 1 cut(s) 564
SaqAI TTAA 2 cut(s) 681, 816
Sau3AI GATC 4 cut(s) 105, 378, 765, 880
Sau96I GGNCC 7 cut(s) 245, 291, 361, 442, 571, 648, 949
SchI GAGTC 3 cut(s) 92, 314, 838
ScrFI CCNGG 4 cut(s) 365, 440, 959, 1042
SduI GDGCHC 3 cut(s) 523, 564, 1148
SfiI GGCCNNNNNGGCC 1 cut(s) 578
Sfr274I CTCGAG 1 cut(s) 350
SfuI TTCGAA 2 cut(s) 830, 1187
SinI GGWCC 2 cut(s) 361, 949
SlaI CTCGAG 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 749
SmlI CTYRAG 4 cut(s) 195, 350, 1023, 1068
SmoI CTYRAG 4 cut(s) 195, 350, 1023, 1068
Sse9I AATT 4 cut(s) 250, 626, 901, 1135
SspMI CTAG 2 cut(s) 369, 539
SstI GAGCTC 1 cut(s) 564
StyD4I CCNGG 4 cut(s) 363, 438, 957, 1040
StyI CCWWGG 4 cut(s) 287, 582, 975, 1075
TaaI ACNGT 1 cut(s) 556
TaiI ACGT 1 cut(s) 1053
TaqI TCGA 7 cut(s) 351, 509, 714, 723, 830, 1053, 1187
TasI AATT 4 cut(s) 250, 626, 901, 1135
TauI GCSGC 9 cut(s) 81, 246, 334, 349, 358, 386, 401, 572, 711
TfiI GAWTC 5 cut(s) 11, 424, 980, 992, 1082
Tru1I TTAA 2 cut(s) 681, 816
Tru9I TTAA 2 cut(s) 681, 816
TscAI CASTG 2 cut(s) 170, 537
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 6 cut(s) 59, 479, 482, 544, 885, 971
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 1 cut(s) 1108
TspGWI ACGGA 4 cut(s) 41, 305, 638, 1191
TspRI CASTG 2 cut(s) 170, 537
Van91I CCANNNNNTGG 2 cut(s) 964, 1140
VpaK11BI GGWCC 2 cut(s) 361, 949
XhoI CTCGAG 1 cut(s) 350
XmnI GAANNNNTTC 2 cut(s) 87, 1183
XspI CTAG 2 cut(s) 369, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.