MD13G1134900.v1.1

Histone-lysine N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002127
Physical Location & Seq
Reverse (-)
10396202 .. 10397436
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1134900.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGGAAATGACGAAGGAATTATTGACGGTAACGGAGATCGAAGGGAGAGGACGGGCGTTAGTGGCCGCTGAGCCACTCAGGGGTGGCGAAATCGTTCTCAGAGACTCTCCCATCCTCCTCTACTCTGCTCTTCCTCTCATCACTCCTTCTCCCCACCCTTACTGCCACCGCTGCTTCAAAACCCTACAAAAACCCCAACCCCAAGCTCAGCCTCAACTATGTCCATGGTGTTCCCACCACGTCTTCTGCGGCCCCAATTGCCTCTCTGCCGCTCTCTCCTCCTCCCACTCTCCTTGGGCCTGCCAAGCCCTGGGCCGTCTCCGAGACCCTTCTTCGCCTCTCTCCGCCCAGCCGCTCGATCGCCAGGTCCAGGCCAGTTTCTTGGTCGCCGCCTACAATCTCGCGGTCGTTTCCCCTTCCAATTTCCAGATTCTATTGTCTCTCCAGGGCCAGCCGCACGACTCCGCCGTTGCCGACACCACCGCAGCTGCCCAGTTTCTTCACTCTCTCATCTCGACACTCTGTGCTCCTCCTCCATTTCTCCTAGAGAAGCAGATGTTTTCGGTAGAGCTCTCGGCGGCTCTACTGGCCAAGGACAAGCTCAACGCCTTTGGATTGATGGAACCCTTCACGGAATTGTCGCAGAGGTCGAGCAGGGCCTACGCCATTTATCCCAAAGCTTCGTTCTTCAACCACGACTGCCTCCCCAATGCCTGCCGGTTTGATTATATCGACACAGACAGCGAACACAACACGGACATGGTGATCAGGATGATACATGATGTTCCGGCAGGGAGGGAGATCTGCCTGAGCTATTTCCCGGTGAATCAGAGTCATTCGAACCGGCAGAGGACTCTGGCGGAGGACTATGGTTTTGCTTGTCAATGTGATCGCTGCAAGGTGGAAGCTAGTTGGTCCGACAATGAGGAGGGTGAGGAAGAAGCAGTGGGTATGGATGAGGACCAAGACCAGGAAATGGAGGCATCCTCGGAATCAGAACCCGAATCCGGGATTGACATTGAAGAAGCCGAAGCCGAAGCCCAAGGGGAAGCCGATTTCCCACACGCCTATTTCTTTGTCAGGTTCATGTGCAACAGAACCAATTGCTGGGGCACCCTGGCTCCATTGCCCCCGAAAGATGACGGAAGTCCTTCGAATGTCATGGAGTGCAATGTCTGTGGCAGCCTTAAGAAAGACGAGCATAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SET

Protein Analysis

403

Amino Acids

44.42

Weight (kDa)

4.78

Isoelectric Point (pI)

57.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 17 - 272 4.4e-08 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1112
AccB7I CCANNNNNTGG 3 cut(s) 310, 976, 1107
AccBSI CCGCTC 2 cut(s) 272, 355
AccII CGCG 1 cut(s) 404
AcoI YGGCCR 2 cut(s) 63, 588
AdeI CACNNNGTG 1 cut(s) 524
AfiI CCNNNNNNNGG 8 cut(s) 80, 310, 370, 970, 976, 1007, 1008, 1107
AflII CTTAAG 1 cut(s) 1187
AgsI TTSAA 3 cut(s) 178, 691, 1022
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 6 cut(s) 309, 363, 369, 444, 969, 1116
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
AluBI AGCT 8 cut(s) 206, 488, 571, 601, 680, 813, 908, 1206
AluI AGCT 8 cut(s) 206, 488, 571, 601, 680, 813, 908, 1206
Alw21I GWGCWC 2 cut(s) 529, 573
Alw26I GTCTC 4 cut(s) 96, 318, 323, 444
AoxI GGCC 8 cut(s) 63, 250, 297, 313, 372, 448, 588, 657
ApeKI GCWGC 5 cut(s) 171, 485, 488, 894, 1182
ArsI GACNNNNNNTTYG 2 cut(s) 857, 889
Asp700I GAANNNNTTC 2 cut(s) 93, 1150
AspS9I GGNCC 8 cut(s) 251, 297, 313, 367, 448, 657, 915, 961
AsuC2I CCSGG 2 cut(s) 821, 1009
AsuHPI GGTGA 3 cut(s) 775, 835, 944
AsuII TTCGAA 2 cut(s) 839, 1154
AvaII GGWCC 3 cut(s) 367, 915, 961
BaeGI GKGCMC 1 cut(s) 1115
BalI TGGCCA 1 cut(s) 590
BanI GGYRCC 1 cut(s) 1112
BanII GRGCYC 1 cut(s) 573
BbsI GAAGAC 1 cut(s) 235
Bbv12I GWGCWC 2 cut(s) 529, 573
BbvI GCAGC 5 cut(s) 158, 475, 497, 881, 1194
BccI CCATC 2 cut(s) 119, 613
BceAI ACGGC 2 cut(s) 300, 452
BciT130I CCWGG 6 cut(s) 311, 365, 371, 446, 971, 1118
BclI TGATCA 1 cut(s) 765
BcnI CCSGG 2 cut(s) 821, 1009
BcoDI GTCTC 4 cut(s) 96, 318, 323, 444
BfaI CTAG 2 cut(s) 545, 909
BfrI CTTAAG 1 cut(s) 1187
BglII AGATCT 1 cut(s) 801
BlpI GCTNAGC 2 cut(s) 69, 207
Bme1390I CCNGG 8 cut(s) 311, 365, 371, 446, 821, 971, 1009, 1118
Bme18I GGWCC 3 cut(s) 367, 915, 961
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 8 cut(s) 251, 297, 313, 367, 448, 657, 915, 961
BmiI GGNNCC 4 cut(s) 253, 624, 1114, 1122
BmrFI CCNGG 8 cut(s) 311, 365, 371, 446, 821, 971, 1009, 1118
BmrI ACTGGG 1 cut(s) 487
BmsI GCATC 1 cut(s) 992
BmuI ACTGGG 1 cut(s) 487
BoxI GACNNNNGTC 1 cut(s) 1146
BpiI GAAGAC 1 cut(s) 235
BplI GAGNNNNNCTC 2 cut(s) 971, 1003
BpmI CTGGAG 1 cut(s) 428
Bpu10I CCTNAGC 1 cut(s) 809
Bpu1102I GCTNAGC 2 cut(s) 69, 207
Bpu14I TTCGAA 2 cut(s) 839, 1154
BpuMI CCSGG 2 cut(s) 821, 1009
BsaI GGTCTC 1 cut(s) 318
BsaJI CCNNGG 9 cut(s) 224, 293, 309, 310, 445, 591, 987, 1042, 1116
BsaXI ACNNNNNCTCC 4 cut(s) 133, 163, 1105, 1135
Bsc4I CCNNNNNNNGG 8 cut(s) 80, 310, 370, 970, 976, 1007, 1008, 1107
Bse118I RCCGGY 2 cut(s) 717, 843
Bse1I ACTGG 3 cut(s) 375, 493, 591
Bse3DI GCAATG 2 cut(s) 1124, 1177
BseBI CCWGG 6 cut(s) 311, 365, 371, 446, 971, 1118
BseDI CCNNGG 9 cut(s) 224, 293, 309, 310, 445, 591, 987, 1042, 1116
BseGI GGATG 4 cut(s) 111, 777, 961, 983
BseLI CCNNNNNNNGG 8 cut(s) 80, 310, 370, 970, 976, 1007, 1008, 1107
BseMI GCAATG 2 cut(s) 1124, 1177
BseMII CTCAG 5 cut(s) 60, 91, 112, 221, 800
BseNI ACTGG 3 cut(s) 375, 493, 591
BseRI GAGGAG 6 cut(s) 107, 268, 271, 519, 522, 941
BseSI GKGCMC 1 cut(s) 1115
BseXI GCAGC 5 cut(s) 158, 475, 497, 881, 1194
BseYI CCCAGC 2 cut(s) 348, 1107
Bsh1236I CGCG 1 cut(s) 404
Bsh1285I CGRYCG 2 cut(s) 361, 408
BshFI GGCC 8 cut(s) 65, 252, 299, 315, 374, 450, 590, 659
BshNI GGYRCC 1 cut(s) 1112
BsiEI CGRYCG 2 cut(s) 361, 408
BsiHKAI GWGCWC 2 cut(s) 529, 573
BsiSI CCGG 5 cut(s) 718, 788, 821, 844, 1008
BslI CCNNNNNNNGG 8 cut(s) 80, 310, 370, 970, 976, 1007, 1008, 1107
BsmAI GTCTC 4 cut(s) 96, 318, 323, 444
BsmBI CGTCTC 1 cut(s) 323
BsnI GGCC 8 cut(s) 65, 252, 299, 315, 374, 450, 590, 659
Bso31I GGTCTC 1 cut(s) 318
Bsp119I TTCGAA 2 cut(s) 839, 1154
Bsp1286I GDGCHC 3 cut(s) 529, 573, 1115
Bsp143I GATC 5 cut(s) 36, 358, 765, 801, 889
Bsp1720I GCTNAGC 2 cut(s) 69, 207
Bsp19I CCATGG 1 cut(s) 224
BspANI GGCC 8 cut(s) 65, 252, 299, 315, 374, 450, 590, 659
BspCNI CTCAG 5 cut(s) 61, 90, 111, 220, 801
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 4 cut(s) 253, 624, 1114, 1122
BspQI GCTCTTC 1 cut(s) 135
BspT104I TTCGAA 2 cut(s) 839, 1154
BspT107I GGYRCC 1 cut(s) 1112
BspTI CTTAAG 1 cut(s) 1187
BspTNI GGTCTC 1 cut(s) 318
BsrBI CCGCTC 2 cut(s) 272, 355
BsrDI GCAATG 2 cut(s) 1124, 1177
BsrFI RCCGGY 2 cut(s) 717, 843
BsrI ACTGG 3 cut(s) 375, 493, 591
BssAI RCCGGY 2 cut(s) 717, 843
BssECI CCNNGG 9 cut(s) 224, 293, 309, 310, 445, 591, 987, 1042, 1116
BssMI GATC 5 cut(s) 36, 358, 765, 801, 889
BssT1I CCWWGG 4 cut(s) 224, 293, 591, 1042
Bst2UI CCWGG 6 cut(s) 311, 365, 371, 446, 971, 1118
Bst4CI ACNGT 1 cut(s) 28
Bst6I CTCTTC 1 cut(s) 135
BstAFI CTTAAG 1 cut(s) 1187
BstBI TTCGAA 2 cut(s) 839, 1154
BstC8I GCNNGC 3 cut(s) 301, 452, 715
BstDEI CTNAG 5 cut(s) 69, 77, 98, 207, 809
BstDSI CCRYGG 1 cut(s) 224
BstF5I GGATG 4 cut(s) 111, 777, 961, 983
BstFNI CGCG 1 cut(s) 404
BstKTI GATC 5 cut(s) 39, 361, 768, 804, 892
BstMAI GTCTC 4 cut(s) 96, 318, 323, 444
BstMBI GATC 5 cut(s) 36, 358, 765, 801, 889
BstMCI CGRYCG 2 cut(s) 361, 408
BstMWI GCNNNNNNNGC 5 cut(s) 62, 171, 258, 305, 587
BstNI CCWGG 6 cut(s) 311, 365, 371, 446, 971, 1118
BstPAI GACNNNNGTC 1 cut(s) 1146
BstSCI CCNGG 8 cut(s) 309, 363, 369, 444, 819, 969, 1007, 1116
BstSLI GKGCMC 1 cut(s) 1115
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 5 cut(s) 158, 475, 497, 881, 1194
BstV2I GAAGAC 1 cut(s) 235
BstX2I RGATCY 1 cut(s) 801
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 1 cut(s) 801
BsuRI GGCC 8 cut(s) 65, 252, 299, 315, 374, 450, 590, 659
BtgI CCRYGG 1 cut(s) 224
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 4 cut(s) 111, 777, 961, 983
BtsI GCAGTG 1 cut(s) 951
BtsIMutI CAGTG 1 cut(s) 951
Cac8I GCNNGC 3 cut(s) 301, 452, 715
Cfr10I RCCGGY 2 cut(s) 717, 843
Cfr13I GGNCC 8 cut(s) 251, 297, 313, 367, 448, 657, 915, 961
CspCI CAANNNNNGTGG 4 cut(s) 275, 310, 1159, 1194
CviAII CATG 5 cut(s) 225, 760, 779, 1087, 1162
DdeI CTNAG 5 cut(s) 69, 77, 98, 207, 809
DpnI GATC 5 cut(s) 38, 360, 767, 803, 891
DpnII GATC 5 cut(s) 36, 358, 765, 801, 889
DraIII CACNNNGTG 1 cut(s) 524
EaeI YGGCCR 2 cut(s) 63, 588
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
EciI GGCGGA 3 cut(s) 334, 454, 875
Ecl136II GAGCTC 1 cut(s) 571
Eco130I CCWWGG 4 cut(s) 224, 293, 591, 1042
Eco24I GRGCYC 1 cut(s) 573
Eco31I GGTCTC 1 cut(s) 318
Eco47I GGWCC 3 cut(s) 367, 915, 961
Eco53kI GAGCTC 1 cut(s) 571
EcoICRI GAGCTC 1 cut(s) 571
EcoO109I RGGNCCY 1 cut(s) 657
EcoRII CCWGG 6 cut(s) 309, 363, 369, 444, 969, 1116
EcoT14I CCWWGG 4 cut(s) 224, 293, 591, 1042
EcoT38I GRGCYC 1 cut(s) 573
ErhI CCWWGG 4 cut(s) 224, 293, 591, 1042
Esp3I CGTCTC 1 cut(s) 323
FaeI CATG 5 cut(s) 228, 763, 782, 1090, 1165
FatI CATG 5 cut(s) 224, 759, 778, 1086, 1161
FbaI TGATCA 1 cut(s) 765
FokI GGATG 4 cut(s) 98, 784, 968, 970
FriOI GRGCYC 1 cut(s) 573
FspBI CTAG 2 cut(s) 545, 909
GsaI CCCAGC 2 cut(s) 352, 1111
GsuI CTGGAG 1 cut(s) 428
HaeIII GGCC 8 cut(s) 65, 252, 299, 315, 374, 450, 590, 659
HapII CCGG 5 cut(s) 718, 788, 821, 844, 1008
Hin1II CATG 5 cut(s) 228, 763, 782, 1090, 1165
HindIII AAGCTT 1 cut(s) 678
HinfI GANTC 8 cut(s) 104, 430, 461, 826, 832, 853, 992, 1004
HpaII CCGG 5 cut(s) 718, 788, 821, 844, 1008
HphI GGTGA 3 cut(s) 775, 835, 944
Hpy188I TCNGA 6 cut(s) 101, 323, 831, 919, 991, 997
Hpy188III TCNNGA 3 cut(s) 427, 514, 769
HpyAV CCTTC 7 cut(s) 7, 35, 156, 339, 426, 637, 1161
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 3 cut(s) 897, 1092, 1170
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 171, 258, 305, 587
HpyF3I CTNAG 5 cut(s) 69, 77, 98, 207, 809
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 5 cut(s) 228, 763, 782, 1090, 1165
Ksp22I TGATCA 1 cut(s) 765
Kzo9I GATC 5 cut(s) 36, 358, 765, 801, 889
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 2 cut(s) 532, 1126
Lsp1109I GCAGC 5 cut(s) 158, 475, 497, 881, 1194
LweI GCATC 1 cut(s) 992
MaeI CTAG 2 cut(s) 545, 909
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 28
MalI GATC 5 cut(s) 38, 360, 767, 803, 891
MbiI CCGCTC 2 cut(s) 272, 355
MboI GATC 5 cut(s) 36, 358, 765, 801, 889
MboII GAAGA 7 cut(s) 122, 235, 324, 491, 679, 950, 1034
MfeI CAATTG 2 cut(s) 256, 1102
MflI RGATCY 1 cut(s) 801
MhlI GDGCHC 3 cut(s) 529, 573, 1115
MlsI TGGCCA 1 cut(s) 590
MluCI AATT 5 cut(s) 17, 256, 421, 635, 1102
MluNI TGGCCA 1 cut(s) 590
MlyI GAGTC 4 cut(s) 98, 455, 841, 847
MmeI TCCRAC 1 cut(s) 942
Mox20I TGGCCA 1 cut(s) 590
MroXI GAANNNNTTC 2 cut(s) 93, 1150
MscI TGGCCA 1 cut(s) 590
MseI TTAA 1 cut(s) 1188
MslI CAYNNNNRTG 1 cut(s) 758
Msp20I TGGCCA 1 cut(s) 590
MspA1I CMGCKG 3 cut(s) 68, 171, 488
MspCI CTTAAG 1 cut(s) 1187
MspI CCGG 5 cut(s) 718, 788, 821, 844, 1008
MspR9I CCNGG 8 cut(s) 311, 365, 371, 446, 821, 971, 1009, 1118
MunI CAATTG 2 cut(s) 256, 1102
MvaI CCWGG 6 cut(s) 311, 365, 371, 446, 971, 1118
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 5 cut(s) 62, 171, 258, 305, 587
NciI CCSGG 2 cut(s) 821, 1009
NcoI CCATGG 1 cut(s) 224
NdeII GATC 5 cut(s) 36, 358, 765, 801, 889
NlaIII CATG 5 cut(s) 228, 763, 782, 1090, 1165
NlaIV GGNNCC 4 cut(s) 253, 624, 1114, 1122
NmeAIII GCCGAG 1 cut(s) 554
NspV TTCGAA 2 cut(s) 839, 1154
PasI CCCWGGG 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 135
PcsI WCGNNNNNNNCGW 2 cut(s) 465, 647
PdmI GAANNNNTTC 2 cut(s) 93, 1150
PfeI GAWTC 4 cut(s) 430, 826, 992, 1004
PflMI CCANNNNNTGG 3 cut(s) 310, 976, 1107
PfoI TCCNGGA 1 cut(s) 1007
Ple19I CGATCG 1 cut(s) 361
PleI GAGTC 4 cut(s) 98, 455, 840, 847
PpsI GAGTC 4 cut(s) 98, 455, 840, 847
PshAI GACNNNNGTC 1 cut(s) 1146
Psp124BI GAGCTC 1 cut(s) 573
Psp6I CCWGG 6 cut(s) 309, 363, 369, 444, 969, 1116
PspFI CCCAGC 2 cut(s) 348, 1107
PspGI CCWGG 6 cut(s) 309, 363, 369, 444, 969, 1116
PspN4I GGNNCC 4 cut(s) 253, 624, 1114, 1122
PspPI GGNCC 8 cut(s) 251, 297, 313, 367, 448, 657, 915, 961
PsuI RGATCY 1 cut(s) 801
PvuI CGATCG 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 488
RseI CAYNNNNRTG 1 cut(s) 758
SacI GAGCTC 1 cut(s) 573
SapI GCTCTTC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 1188
Sau3AI GATC 5 cut(s) 36, 358, 765, 801, 889
Sau96I GGNCC 8 cut(s) 251, 297, 313, 367, 448, 657, 915, 961
SchI GAGTC 4 cut(s) 98, 455, 841, 847
ScrFI CCNGG 8 cut(s) 311, 365, 371, 446, 821, 971, 1009, 1118
SduI GDGCHC 3 cut(s) 529, 573, 1115
SfaNI GCATC 1 cut(s) 992
SfuI TTCGAA 2 cut(s) 839, 1154
SinI GGWCC 3 cut(s) 367, 915, 961
SmiMI CAYNNNNRTG 1 cut(s) 758
SmlI CTYRAG 1 cut(s) 1187
SmoI CTYRAG 1 cut(s) 1187
Sse9I AATT 5 cut(s) 17, 256, 421, 635, 1102
SspMI CTAG 2 cut(s) 545, 909
SstI GAGCTC 1 cut(s) 573
StyD4I CCNGG 8 cut(s) 309, 363, 369, 444, 819, 969, 1007, 1116
StyI CCWWGG 4 cut(s) 224, 293, 591, 1042
TaaI ACNGT 1 cut(s) 28
TaiI ACGT 1 cut(s) 243
TaqI TCGA 7 cut(s) 39, 357, 515, 650, 732, 839, 1154
TasI AATT 5 cut(s) 17, 256, 421, 635, 1102
TauI GCSGC 7 cut(s) 68, 252, 272, 355, 392, 457, 581
TfiI GAWTC 4 cut(s) 430, 826, 992, 1004
Tru1I TTAA 1 cut(s) 1188
Tru9I TTAA 1 cut(s) 1188
TscAI CASTG 1 cut(s) 951
TseI GCWGC 5 cut(s) 171, 485, 488, 894, 1182
TspDTI ATGAA 1 cut(s) 1075
TspGWI ACGGA 4 cut(s) 47, 647, 770, 1158
TspRI CASTG 1 cut(s) 951
Van91I CCANNNNNTGG 3 cut(s) 310, 976, 1107
Vha464I CTTAAG 1 cut(s) 1187
VpaK11BI GGWCC 3 cut(s) 367, 915, 961
XmnI GAANNNNTTC 2 cut(s) 93, 1150
XspI CTAG 2 cut(s) 545, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.