FvH4_4g22660

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000847
Physical Location & Seq
Reverse (-)
25385615 .. 25390162
4548 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22660.t8

Sequence Viewer

Length: 1350 bp
ATGGGAAAAAGTCGAGGCAAGGGTTTAAGCAAAAGGGTCCTAACTATGGATCATGATGGTAGGATTAGGAAGATAGAGAACAGTTTTACAAAGAAAAAGAAGAAATCTAAGAAGATAAGTGAATCTTCCATCCCCGAGACAGCAGCTGAGCTTACTAGAGGCAACTCTGCTTCCAATGTCACTAAGGCAACTTCTTCTAGTCATGCTAATCGAGCTACTAATGATAGTGGGTGGTCATTTTCTGGTTTTATATTTATGTGTAATGGAAAGACCAAACCTGAATGTTATCAGAATCGTGTTTTTGGGATGCCACTGCCAAAAGAAGAAGTTGTAAAGACGATCAAACAGGGTACACATCTGTTTTTATATGACTTTGACTTGAAGCTCCTTTATGGCACATATGAGGCAACATCAGATGGAGCATTAGATTTGGAGCCAATTGCGTTCAAGGGAAAATTTCCTGCACAGGTCAGGTTTAAGATTTCCAAGGATTGTTTACCGCTTCACGAAAGTTCCTTTAGAGCAGCCATAAGAGACAATTACCAGGGTGGTAAGTTTAAACAAGAGCTGAATAAAAAACAGGTGCGTGCTCTATACTCTATGTTCCGCCCAATCAAAGCGTCAATTCCTACTTCTGCTGCCCATCACCAAGATGTGGCAAGAATAGGCTCATATCAATCTCCTATTGGGGAAGAAGGATTTTATCCAACAGCAAGCCCGTGTCTACAAGAGAGCTTATATTTATCTAGTGTACAACGCACTCATGGATTGCCACTTCTTCGTATGCAATCTAAACAGGTTGTCCCATATCCACAGTATGATAAATATGAAGCGGAAGAGTATGTGGCTCATGTTCAGCCTCCCATAGAGCCTTGGCATGTTGTTCAGCATCATGCAGATTCGTATTCTCTAGCAGCCGCCCATCAGCCATATGTGCCTGAGAAGTCTTTCCTGTCCTCCCATGATGCACATAGAAGGCAAGGGGTCACGCATTCACAGGAGATGGTTCCTAGGGATCAAGTTATTTCATATGGAAGCGTGTATCACTTGTCACAGTTGCTGAAGGAGAGAGAAAGAGACATTGTCTCCCATTCTGATTATATTGCGGAAACATATAGTCGACAGTTGCCAACTCTTGCAGCGTCACAGTTTAGGTCACAATCAGATTCTGCGGCATCATCTTATGAGTTACCGCCAACTTACCAACCGTATTATCAAGCAACGACCCATCACGATCAGAACCGGGTGGATCATGATCCTCTTGAGAGACCATTACATGGAAGCTCTAATGTGACAGATATGCCAATCTATTCTCACTTGTCTTCCACCGGAGCTACGCGCTACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

450

Amino Acids

50.77

Weight (kDa)

9.12

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 2 - 100 3.1e-35 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 655, 1277
AccI GTMKAC 2 cut(s) 724, 1120
AccII CGCG 1 cut(s) 1339
AciI CCGC 7 cut(s) 500, 607, 833, 918, 1106, 1172, 1193
AclWI GGATC 4 cut(s) 57, 1023, 1250, 1257
AcsI RAATTY 1 cut(s) 455
AcuI CTGAAG 1 cut(s) 1082
AfaI GTAC 2 cut(s) 352, 753
AfiI CCNNNNNNNGG 3 cut(s) 46, 655, 1277
AgsI TTSAA 2 cut(s) 382, 448
AjnI CCWGG 1 cut(s) 543
AluBI AGCT 8 cut(s) 146, 151, 215, 385, 568, 735, 1284, 1334
AluI AGCT 8 cut(s) 146, 151, 215, 385, 568, 735, 1284, 1334
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 5 cut(s) 131, 528, 1071, 1090, 1261
AlwI GGATC 4 cut(s) 57, 1023, 1250, 1257
AlwNI CAGNNNCTG 3 cut(s) 146, 1060, 1169
Ama87I CYCGRG 1 cut(s) 134
ApeKI GCWGC 5 cut(s) 143, 524, 638, 914, 1139
ApoI RAATTY 1 cut(s) 455
Asp700I GAANNNNTTC 1 cut(s) 947
AspA2I CCTAGG 1 cut(s) 1010
AspLEI GCGC 1 cut(s) 1341
AspS9I GGNCC 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 1244
AsuHPI GGTGA 1 cut(s) 638
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 1 cut(s) 37
AvrII CCTAGG 1 cut(s) 1010
BbsI GAAGAC 1 cut(s) 1314
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 5 cut(s) 155, 536, 625, 926, 1151
BccI CCATC 7 cut(s) 50, 137, 410, 651, 930, 997, 1236
BciT130I CCWGG 1 cut(s) 545
BcnI CCSGG 1 cut(s) 1244
BcoDI GTCTC 5 cut(s) 131, 528, 1071, 1090, 1261
BfaI CTAG 5 cut(s) 156, 198, 747, 911, 1011
BisI GCNGC 7 cut(s) 144, 525, 639, 915, 918, 1140, 1173
BlnI CCTAGG 1 cut(s) 1010
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 7 cut(s) 145, 526, 640, 916, 919, 1141, 1174
Bme1390I CCNGG 2 cut(s) 545, 1244
Bme18I GGWCC 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 3 cut(s) 38, 435, 1008
BmrFI CCNGG 2 cut(s) 545, 1244
BmsI GCATC 4 cut(s) 297, 898, 955, 1184
BpiI GAAGAC 1 cut(s) 1314
Bpu1102I GCTNAGC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 1283
BpuMI CCSGG 1 cut(s) 1244
BsaBI GATNNNNATC 1 cut(s) 1254
BsaI GGTCTC 1 cut(s) 1261
BsaJI CCNNGG 4 cut(s) 486, 544, 872, 1010
BsaWI WCCGGW 1 cut(s) 1328
BsaXI ACNNNNNCTCC 2 cut(s) 1070, 1100
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 655, 1277
Bse8I GATNNNNATC 1 cut(s) 1254
BseBI CCWGG 1 cut(s) 545
BseDI CCNNGG 4 cut(s) 486, 544, 872, 1010
BseGI GGATG 2 cut(s) 129, 312
BseJI GATNNNNATC 1 cut(s) 1254
BseLI CCNNNNNNNGG 3 cut(s) 46, 655, 1277
BseMII CTCAG 2 cut(s) 138, 930
BseXI GCAGC 5 cut(s) 155, 536, 625, 926, 1151
BsgI GTGCAG 1 cut(s) 447
Bsh1236I CGCG 1 cut(s) 1339
BsiHKAI GWGCWC 1 cut(s) 592
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 2 cut(s) 1243, 1329
BslFI GGGAC 1 cut(s) 788
BslI CCNNNNNNNGG 3 cut(s) 46, 655, 1277
BsmAI GTCTC 5 cut(s) 131, 528, 1071, 1090, 1261
BsmFI GGGAC 1 cut(s) 788
BsmI GAATGC 1 cut(s) 991
Bso31I GGTCTC 1 cut(s) 1261
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 592
Bsp1407I TGTACA 1 cut(s) 751
Bsp143I GATC 6 cut(s) 49, 339, 1015, 1234, 1249, 1255
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 7 cut(s) 500, 607, 833, 918, 1106, 1172, 1193
BspCNI CTCAG 2 cut(s) 139, 931
BspFNI CGCG 1 cut(s) 1339
BspHI TCATGA 2 cut(s) 52, 1252
BspLI GGNNCC 3 cut(s) 38, 435, 1008
BspPI GGATC 4 cut(s) 57, 1023, 1250, 1257
BspTNI GGTCTC 1 cut(s) 1261
BsrGI TGTACA 1 cut(s) 751
BssECI CCNNGG 4 cut(s) 486, 544, 872, 1010
BssMI GATC 6 cut(s) 49, 339, 1015, 1234, 1249, 1255
BssT1I CCWWGG 3 cut(s) 486, 872, 1010
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 6 cut(s) 83, 816, 1056, 1125, 1149, 1209
Bst6I CTCTTC 1 cut(s) 831
BstAUI TGTACA 1 cut(s) 751
BstC8I GCNNGC 2 cut(s) 588, 715
BstDEI CTNAG 4 cut(s) 108, 147, 183, 939
BstF5I GGATG 2 cut(s) 129, 312
BstFNI CGCG 1 cut(s) 1339
BstHHI GCGC 1 cut(s) 1341
BstKTI GATC 6 cut(s) 52, 342, 1018, 1237, 1252, 1258
BstMAI GTCTC 5 cut(s) 131, 528, 1071, 1090, 1261
BstMBI GATC 6 cut(s) 49, 339, 1015, 1234, 1249, 1255
BstMWI GCNNNNNNNGC 2 cut(s) 212, 934
BstNI CCWGG 1 cut(s) 545
BstNSI RCATGY 1 cut(s) 881
BstSCI CCNGG 2 cut(s) 543, 1242
BstUI CGCG 1 cut(s) 1339
BstV1I GCAGC 5 cut(s) 155, 536, 625, 926, 1151
BstV2I GAAGAC 1 cut(s) 1314
BtsCI GGATG 2 cut(s) 129, 312
BtsI GCAGTG 1 cut(s) 311
BtsIMutI CAGTG 1 cut(s) 311
Cac8I GCNNGC 2 cut(s) 588, 715
CaiI CAGNNNCTG 3 cut(s) 146, 1060, 1169
CciI TCATGA 2 cut(s) 52, 1252
CfoI GCGC 1 cut(s) 1341
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 2 cut(s) 609, 1131
Csp6I GTAC 2 cut(s) 351, 752
CviAII CATG 9 cut(s) 53, 203, 764, 851, 878, 893, 962, 1253, 1277
CviQI GTAC 2 cut(s) 351, 752
DdeI CTNAG 4 cut(s) 108, 147, 183, 939
DpnI GATC 6 cut(s) 51, 341, 1017, 1236, 1251, 1257
DpnII GATC 6 cut(s) 49, 339, 1015, 1234, 1249, 1255
DraI TTTAAA 1 cut(s) 559
Eam1104I CTCTTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 831
EciI GGCGGA 1 cut(s) 596
Eco130I CCWWGG 3 cut(s) 486, 872, 1010
Eco31I GGTCTC 1 cut(s) 1261
Eco47I GGWCC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 1082
Eco88I CYCGRG 1 cut(s) 134
EcoO109I RGGNCCY 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 543
EcoT14I CCWWGG 3 cut(s) 486, 872, 1010
ErhI CCWWGG 3 cut(s) 486, 872, 1010
FaeI CATG 9 cut(s) 56, 206, 767, 854, 881, 896, 965, 1256, 1280
FalI AAGNNNNNCTT 2 cut(s) 109, 141
FaqI GGGAC 1 cut(s) 788
FatI CATG 9 cut(s) 52, 202, 763, 850, 877, 892, 961, 1252, 1276
FauNDI CATATG 3 cut(s) 400, 931, 1030
FblI GTMKAC 2 cut(s) 724, 1120
Fnu4HI GCNGC 7 cut(s) 144, 525, 639, 915, 918, 1140, 1173
FokI GGATG 2 cut(s) 116, 319
Fsp4HI GCNGC 7 cut(s) 144, 525, 639, 915, 918, 1140, 1173
FspBI CTAG 5 cut(s) 156, 198, 747, 911, 1011
GlaI GCGC 1 cut(s) 1340
GluI GCNGC 7 cut(s) 144, 525, 639, 915, 918, 1140, 1173
HapII CCGG 2 cut(s) 1243, 1329
HgaI GACGC 2 cut(s) 609, 1131
HhaI GCGC 1 cut(s) 1341
Hin1II CATG 9 cut(s) 56, 206, 767, 854, 881, 896, 965, 1256, 1280
Hin6I GCGC 1 cut(s) 1339
HinP1I GCGC 1 cut(s) 1339
HincII GTYRAC 1 cut(s) 1121
HindII GTYRAC 1 cut(s) 1121
HinfI GANTC 4 cut(s) 122, 292, 899, 1166
HpaII CCGG 2 cut(s) 1243, 1329
HphI GGTGA 1 cut(s) 638
Hpy166II GTNNAC 5 cut(s) 353, 497, 725, 752, 1121
Hpy188I TCNGA 5 cut(s) 291, 415, 1096, 1165, 1239
Hpy188III TCNNGA 5 cut(s) 53, 506, 1232, 1253, 1262
Hpy8I GTNNAC 5 cut(s) 353, 497, 725, 752, 1121
HpyAV CCTTC 3 cut(s) 689, 969, 1057
HpyCH4III ACNGT 6 cut(s) 83, 816, 1056, 1125, 1149, 1209
HpyCH4V TGCA 5 cut(s) 464, 787, 896, 968, 1139
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 934
HpyF3I CTNAG 4 cut(s) 108, 147, 183, 939
Hsp92II CATG 9 cut(s) 56, 206, 767, 854, 881, 896, 965, 1256, 1280
HspAI GCGC 1 cut(s) 1339
Kzo9I GATC 6 cut(s) 49, 339, 1015, 1234, 1249, 1255
LmnI GCTCC 4 cut(s) 390, 419, 433, 1331
Lsp1109I GCAGC 5 cut(s) 155, 536, 625, 926, 1151
LweI GCATC 4 cut(s) 297, 898, 955, 1184
MaeI CTAG 5 cut(s) 156, 198, 747, 911, 1011
MaeIII GTNAC 7 cut(s) 178, 985, 1050, 1143, 1155, 1188, 1291
MalI GATC 6 cut(s) 51, 341, 1017, 1236, 1251, 1257
MboI GATC 6 cut(s) 49, 339, 1015, 1234, 1249, 1255
MfeI CAATTG 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 592
MluCI AATT 4 cut(s) 438, 455, 538, 624
MmeI TCCRAC 1 cut(s) 731
MnlI CCTC 6 cut(s) 8, 152, 397, 870, 967, 1269
MroXI GAANNNNTTC 1 cut(s) 947
MseI TTAA 3 cut(s) 26, 477, 558
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 2 cut(s) 1243, 1329
MspR9I CCNGG 2 cut(s) 545, 1244
MssI GTTTAAAC 1 cut(s) 559
MunI CAATTG 1 cut(s) 438
Mva1269I GAATGC 1 cut(s) 991
MvaI CCWGG 1 cut(s) 545
MvnI CGCG 1 cut(s) 1339
MwoI GCNNNNNNNGC 2 cut(s) 212, 934
NciI CCSGG 1 cut(s) 1244
NdeI CATATG 3 cut(s) 400, 931, 1030
NdeII GATC 6 cut(s) 49, 339, 1015, 1234, 1249, 1255
NlaIII CATG 9 cut(s) 56, 206, 767, 854, 881, 896, 965, 1256, 1280
NlaIV GGNNCC 3 cut(s) 38, 435, 1008
NmuCI GTSAC 6 cut(s) 178, 985, 1050, 1143, 1155, 1291
NspI RCATGY 1 cut(s) 881
PagI TCATGA 2 cut(s) 52, 1252
PctI GAATGC 1 cut(s) 991
PdmI GAANNNNTTC 1 cut(s) 947
PfeI GAWTC 4 cut(s) 122, 292, 899, 1166
PflFI GACNNNGTC 1 cut(s) 1082
PflMI CCANNNNNTGG 2 cut(s) 655, 1277
PkrI GCNGC 7 cut(s) 145, 526, 640, 916, 919, 1141, 1174
PmeI GTTTAAAC 1 cut(s) 559
PpuMI RGGWCCY 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 543
PspGI CCWGG 1 cut(s) 543
PspN4I GGNNCC 3 cut(s) 38, 435, 1008
PspPI GGNCC 1 cut(s) 37
PspPPI RGGWCCY 1 cut(s) 37
PstNI CAGNNNCTG 3 cut(s) 146, 1060, 1169
PsyI GACNNNGTC 1 cut(s) 1082
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 2 cut(s) 352, 753
RsaNI GTAC 2 cut(s) 351, 752
RseI CAYNNNNRTG 1 cut(s) 651
SalI GTCGAC 1 cut(s) 1119
SaqAI TTAA 3 cut(s) 26, 477, 558
SatI GCNGC 7 cut(s) 144, 525, 639, 915, 918, 1140, 1173
Sau3AI GATC 6 cut(s) 49, 339, 1015, 1234, 1249, 1255
Sau96I GGNCC 1 cut(s) 37
ScrFI CCNGG 2 cut(s) 545, 1244
SduI GDGCHC 1 cut(s) 592
SfaNI GCATC 4 cut(s) 297, 898, 955, 1184
SinI GGWCC 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 651
SmlI CTYRAG 1 cut(s) 1262
SmoI CTYRAG 1 cut(s) 1262
Sse9I AATT 4 cut(s) 438, 455, 538, 624
SsiI CCGC 7 cut(s) 500, 607, 833, 918, 1106, 1172, 1193
SspMI CTAG 5 cut(s) 156, 198, 747, 911, 1011
StyD4I CCNGG 2 cut(s) 543, 1242
StyI CCWWGG 3 cut(s) 486, 872, 1010
TaaI ACNGT 6 cut(s) 83, 816, 1056, 1125, 1149, 1209
TaqI TCGA 3 cut(s) 13, 211, 1120
TasI AATT 4 cut(s) 438, 455, 538, 624
TatI WGTACW 1 cut(s) 751
TauI GCSGC 2 cut(s) 920, 1175
TfiI GAWTC 4 cut(s) 122, 292, 899, 1166
Tru1I TTAA 3 cut(s) 26, 477, 558
Tru9I TTAA 3 cut(s) 26, 477, 558
TscAI CASTG 1 cut(s) 318
TseFI GTSAC 6 cut(s) 178, 985, 1050, 1143, 1155, 1291
TseI GCWGC 5 cut(s) 143, 524, 638, 914, 1139
Tsp45I GTSAC 6 cut(s) 178, 985, 1050, 1143, 1155, 1291
TspDTI ATGAA 2 cut(s) 843, 1017
TspRI CASTG 1 cut(s) 318
Tth111I GACNNNGTC 1 cut(s) 1082
Van91I CCANNNNNTGG 2 cut(s) 655, 1277
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 455
XceI RCATGY 1 cut(s) 881
XmaJI CCTAGG 1 cut(s) 1010
XmiI GTMKAC 2 cut(s) 724, 1120
XmnI GAANNNNTTC 1 cut(s) 947
XspI CTAG 5 cut(s) 156, 198, 747, 911, 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.